Thrips-associated genomovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus echiam1

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YT70|A0A1P8YT70_9VIRU RepA OS=Thrips-associated genomovirus 1 OX=1941233 PE=3 SV=1
MM1 pKa = 7.97PPFTYY6 pKa = 9.91NARR9 pKa = 11.84YY10 pKa = 9.11FLVTYY15 pKa = 7.74AQCGDD20 pKa = 3.98LDD22 pKa = 3.6PWAVNDD28 pKa = 4.06HH29 pKa = 6.05FASLGAEE36 pKa = 3.95CIVARR41 pKa = 11.84EE42 pKa = 4.04SHH44 pKa = 6.5ADD46 pKa = 3.41EE47 pKa = 4.79GTHH50 pKa = 6.18LHH52 pKa = 6.43AFVDD56 pKa = 4.82FGRR59 pKa = 11.84KK60 pKa = 8.17FRR62 pKa = 11.84SRR64 pKa = 11.84NARR67 pKa = 11.84KK68 pKa = 9.06FDD70 pKa = 3.3VDD72 pKa = 3.22GRR74 pKa = 11.84HH75 pKa = 6.35ANIAPSKK82 pKa = 9.24GKK84 pKa = 9.72PEE86 pKa = 3.91GGYY89 pKa = 10.69DD90 pKa = 3.55YY91 pKa = 10.74AIKK94 pKa = 10.94DD95 pKa = 3.59GDD97 pKa = 4.02VVAGGLARR105 pKa = 11.84PSVNGAVSNGDD116 pKa = 2.92KK117 pKa = 10.14WSEE120 pKa = 3.78IVGAEE125 pKa = 3.99SEE127 pKa = 4.41QLFWEE132 pKa = 4.43AVEE135 pKa = 4.52RR136 pKa = 11.84LDD138 pKa = 5.48PKK140 pKa = 10.91ALCTNYY146 pKa = 10.69GNLRR150 pKa = 11.84KK151 pKa = 9.28FADD154 pKa = 2.71WRR156 pKa = 11.84YY157 pKa = 8.5RR158 pKa = 11.84VEE160 pKa = 4.02PEE162 pKa = 4.24RR163 pKa = 11.84YY164 pKa = 8.04EE165 pKa = 4.33HH166 pKa = 6.82PAGLNFEE173 pKa = 4.91LGMVPDD179 pKa = 4.23LAAWGGFGSGDD190 pKa = 3.45DD191 pKa = 3.82TVGGRR196 pKa = 11.84VRR198 pKa = 11.84SLCLYY203 pKa = 10.7GPTKK207 pKa = 10.49VGKK210 pKa = 5.85TTWARR215 pKa = 11.84SLGNHH220 pKa = 5.79VYY222 pKa = 10.79FMGLMSGEE230 pKa = 4.06VALRR234 pKa = 11.84DD235 pKa = 3.82MPDD238 pKa = 2.72AEE240 pKa = 4.32YY241 pKa = 11.01AVFDD245 pKa = 4.67DD246 pKa = 3.76MRR248 pKa = 11.84GGIKK252 pKa = 10.28FFQAWKK258 pKa = 9.36EE259 pKa = 3.87WFGAQLVVTVKK270 pKa = 10.52KK271 pKa = 10.68LYY273 pKa = 10.05RR274 pKa = 11.84DD275 pKa = 3.54PVQVKK280 pKa = 8.69WGKK283 pKa = 9.01PCIWLCNEE291 pKa = 4.51DD292 pKa = 3.92PRR294 pKa = 11.84TSLEE298 pKa = 3.72PAEE301 pKa = 4.57IDD303 pKa = 3.53WIEE306 pKa = 4.14GNCDD310 pKa = 3.96FIDD313 pKa = 3.57VHH315 pKa = 8.16SSIISHH321 pKa = 6.95ANTT324 pKa = 3.53

Molecular weight: 36.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8YT68|A0A1P8YT68_9VIRU Replication-associated protein OS=Thrips-associated genomovirus 1 OX=1941233 PE=3 SV=1
MM1 pKa = 7.6AYY3 pKa = 10.17ARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84YY8 pKa = 9.77GSYY11 pKa = 9.84RR12 pKa = 11.84AKK14 pKa = 9.83KK15 pKa = 4.35TTKK18 pKa = 9.67RR19 pKa = 11.84SGRR22 pKa = 11.84RR23 pKa = 11.84SPYY26 pKa = 6.97TAKK29 pKa = 9.5TRR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 9.75KK34 pKa = 10.55KK35 pKa = 8.02RR36 pKa = 11.84TYY38 pKa = 10.45RR39 pKa = 11.84KK40 pKa = 7.96RR41 pKa = 11.84TMSTKK46 pKa = 10.53SILNKK51 pKa = 10.02SSRR54 pKa = 11.84KK55 pKa = 9.13KK56 pKa = 10.28RR57 pKa = 11.84NGMLTFSNSTSSGALATVAQNPLTIAGSPSGDD89 pKa = 3.5TIGLVHH95 pKa = 6.83FRR97 pKa = 11.84PTAMDD102 pKa = 4.17LDD104 pKa = 4.69DD105 pKa = 5.5NVTNPNSIVATPSRR119 pKa = 11.84TATVCFMRR127 pKa = 11.84GFLEE131 pKa = 4.38NIRR134 pKa = 11.84VEE136 pKa = 4.41TSSGNPWFHH145 pKa = 6.83RR146 pKa = 11.84RR147 pKa = 11.84VCICSRR153 pKa = 11.84DD154 pKa = 3.64PRR156 pKa = 11.84LLLLSSSDD164 pKa = 3.25TTGVDD169 pKa = 2.2RR170 pKa = 11.84GYY172 pKa = 10.78AASGSIEE179 pKa = 4.24TSNGWQRR186 pKa = 11.84LASNVLTDD194 pKa = 3.41TMTNTTTNWLGLLFKK209 pKa = 10.55GAQGVDD215 pKa = 2.56WDD217 pKa = 4.99DD218 pKa = 4.9YY219 pKa = 10.03ITAPVDD225 pKa = 3.35TTRR228 pKa = 11.84VDD230 pKa = 3.78LKK232 pKa = 10.99FDD234 pKa = 3.36KK235 pKa = 10.49TWIYY239 pKa = 11.12KK240 pKa = 10.19SGNQQGILKK249 pKa = 7.55EE250 pKa = 4.35TKK252 pKa = 9.08LWHH255 pKa = 5.79PMNRR259 pKa = 11.84NLYY262 pKa = 10.35YY263 pKa = 10.76DD264 pKa = 4.47DD265 pKa = 5.48DD266 pKa = 4.72EE267 pKa = 6.32NGAGEE272 pKa = 4.23TTRR275 pKa = 11.84NFSVQDD281 pKa = 3.29KK282 pKa = 10.75RR283 pKa = 11.84GMGDD287 pKa = 2.91YY288 pKa = 10.63HH289 pKa = 8.66IFDD292 pKa = 5.17LFSQGSSGSTADD304 pKa = 3.48RR305 pKa = 11.84LKK307 pKa = 10.67IRR309 pKa = 11.84FTSTMYY315 pKa = 9.45WHH317 pKa = 7.08EE318 pKa = 4.17KK319 pKa = 9.5

Molecular weight: 36.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

853

210

324

284.3

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.441 ± 1.237

1.524 ± 0.326

7.268 ± 0.307

4.807 ± 1.137

4.572 ± 0.496

9.613 ± 0.928

2.462 ± 0.391

3.165 ± 0.377

5.158 ± 0.506

6.682 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.381

4.689 ± 0.435

4.455 ± 0.575

1.641 ± 0.254

7.62 ± 0.85

6.8 ± 1.418

6.213 ± 2.344

6.213 ± 0.973

2.814 ± 0.316

3.869 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski