Ophiocordyceps camponoti-leonardi (nom. inval.)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7043 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369GQ40|A0A369GQ40_9HYPO Uncharacterized protein OS=Ophiocordyceps camponoti-leonardi (nom. inval.) OX=2039875 GN=CP532_1056 PE=4 SV=1
MM1 pKa = 7.16 VSWEE5 pKa = 4.29 RR6 pKa = 11.84 DD7 pKa = 3.35 FKK9 pKa = 11.0 MEE11 pKa = 4.16 EE12 pKa = 3.35 VWYY15 pKa = 9.98 VCGVCGLEE23 pKa = 4.99 HH24 pKa = 7.96 DD25 pKa = 4.68 DD26 pKa = 3.67 AQHH29 pKa = 6.67 RR30 pKa = 11.84 STYY33 pKa = 9.78 RR34 pKa = 11.84 CYY36 pKa = 10.9 VDD38 pKa = 5.33 HH39 pKa = 7.37 FNDD42 pKa = 5.77 DD43 pKa = 4.99 DD44 pKa = 7.09 DD45 pKa = 7.56 DD46 pKa = 7.2 DD47 pKa = 7.56 DD48 pKa = 7.55 DD49 pKa = 7.23 DD50 pKa = 5.91 DD51 pKa = 4.25 VHH53 pKa = 7.18 NNHH56 pKa = 6.46 NDD58 pKa = 3.55 DD59 pKa = 5.3 GDD61 pKa = 5.17 DD62 pKa = 4.77 DD63 pKa = 5.73 DD64 pKa = 5.83 EE65 pKa = 5.33 NSNNDD70 pKa = 3.12 EE71 pKa = 4.08
Molecular weight: 8.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.439
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.808
Patrickios 0.998
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A369GHF9|A0A369GHF9_9HYPO ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Ophiocordyceps camponoti-leonardi (nom. inval.) OX=2039875 GN=CP532_5202 PE=3 SV=1
DDD2 pKa = 3.39 KK3 pKa = 10.82 KK4 pKa = 10.54 KK5 pKa = 10.54 KKK7 pKa = 9.16 KKK9 pKa = 9.83 KK10 pKa = 9.21 SLLASILIILRR21 pKa = 11.84 TSLEEE26 pKa = 3.95 GRR28 pKa = 11.84 FPAFCALLVGGSSLLHHH45 pKa = 6.45 KK46 pKa = 10.21 LTTIVTRR53 pKa = 11.84 VARR56 pKa = 11.84 PRR58 pKa = 11.84 LSNAACLSLARR69 pKa = 11.84 WLASFVASWLSLGLLQKKK87 pKa = 10.35 RR88 pKa = 11.84 PSPLRR93 pKa = 11.84 RR94 pKa = 11.84 EEE96 pKa = 4.23 EE97 pKa = 4.33 PPASSGLVPNKKK109 pKa = 10.16 DD110 pKa = 3.79 DD111 pKa = 3.27 EE112 pKa = 4.66 MAGKKK117 pKa = 8.14 LDDD120 pKa = 3.52 TLFAVTQALDDD131 pKa = 3.8 IIGEEE136 pKa = 3.97 WSRR139 pKa = 11.84 RR140 pKa = 11.84 KKK142 pKa = 9.81 RR143 pKa = 11.84 RR144 pKa = 11.84 VAARR148 pKa = 11.84 KKK150 pKa = 9.03 SDDD153 pKa = 3.17 DDD155 pKa = 4.51 DD156 pKa = 5.26 ISRR159 pKa = 11.84 MADDD163 pKa = 2.91 MSFVASCAFIMWAWFYYY180 pKa = 11.59 PDDD183 pKa = 3.94 LPRR186 pKa = 11.84 SYYY189 pKa = 11.55 KKK191 pKa = 9.85 ITSAAHHH198 pKa = 5.06 DDD200 pKa = 3.65 RR201 pKa = 11.84 LLEEE205 pKa = 3.91 LRR207 pKa = 11.84 RR208 pKa = 11.84 CRR210 pKa = 11.84 TGEEE214 pKa = 3.62 RR215 pKa = 11.84 YYY217 pKa = 10.22 VDDD220 pKa = 2.96 GQAPLLGSMCADDD233 pKa = 3.32 YY234 pKa = 11.12 LPRR237 pKa = 11.84 QWGDDD242 pKa = 3.16 AVSVPFPCDDD252 pKa = 3.16 VHHH255 pKa = 6.82 GRR257 pKa = 11.84 GPCCEEE263 pKa = 3.6 YY264 pKa = 10.47 HH265 pKa = 5.13 WRR267 pKa = 11.84 RR268 pKa = 11.84 FSQSWLWSMYYY279 pKa = 10.02 YYY281 pKa = 11.12 PLAAALQLRR290 pKa = 11.84 RR291 pKa = 11.84 PTLRR295 pKa = 11.84 SPFKKK300 pKa = 10.7 VVSAARR306 pKa = 11.84 SSAFLATFITLFYYY320 pKa = 11.04 YY321 pKa = 8.7 VCLGRR326 pKa = 11.84 TRR328 pKa = 11.84 LGPHHH333 pKa = 6.58 LGRR336 pKa = 11.84 GPSSCDDD343 pKa = 3.29 IDDD346 pKa = 4.68 GICVANGCFLCGWSILIEEE365 pKa = 4.17 TSRR368 pKa = 11.84 RR369 pKa = 11.84 KKK371 pKa = 10.68 DD372 pKa = 3.26 ALFAAPRR379 pKa = 11.84 AMATLLPRR387 pKa = 11.84 RR388 pKa = 11.84 FPTGLQWRR396 pKa = 11.84 EEE398 pKa = 3.7 LAFAASTAVVFTCVQEEE415 pKa = 3.93 PKKK418 pKa = 10.01 VRR420 pKa = 11.84 GMLGGILSLAMRR432 pKa = 11.84 Q
Molecular weight: 48.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.487
IPC_protein 10.131
Toseland 10.438
ProMoST 10.145
Dawson 10.57
Bjellqvist 10.306
Wikipedia 10.774
Rodwell 10.774
Grimsley 10.628
Solomon 10.657
Lehninger 10.628
Nozaki 10.496
DTASelect 10.277
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.511
Patrickios 10.423
IPC_peptide 10.657
IPC2_peptide 9.619
IPC2.peptide.svr19 8.498
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6963
80
7043
3884336
36
5063
551.5
60.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.399 ± 0.028
1.286 ± 0.011
6.219 ± 0.021
5.99 ± 0.03
3.452 ± 0.019
7.118 ± 0.038
2.4 ± 0.014
4.028 ± 0.018
4.473 ± 0.024
8.977 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.011
3.144 ± 0.016
6.196 ± 0.034
3.974 ± 0.022
7.049 ± 0.025
8.345 ± 0.036
5.523 ± 0.018
6.409 ± 0.02
1.373 ± 0.01
2.428 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here