Sulfitobacter sp. JL08
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4063 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345RBX5|A0A345RBX5_9RHOB ABC transporter permease OS=Sulfitobacter sp. JL08 OX=2070369 GN=C1J05_11680 PE=3 SV=1
MM1 pKa = 7.55 AAILTVAFAAPTTAQVATDD20 pKa = 4.27 CPNPDD25 pKa = 4.92 LYY27 pKa = 11.57 SGDD30 pKa = 3.76 YY31 pKa = 10.83 FLTCNEE37 pKa = 3.94 FQGSVKK43 pKa = 9.49 EE44 pKa = 4.32 TTQAEE49 pKa = 4.26 NVTYY53 pKa = 10.99 LEE55 pKa = 4.43 GTDD58 pKa = 4.04 TITGLDD64 pKa = 3.36 NSQPGFTISNSYY76 pKa = 10.27 FLYY79 pKa = 10.85 DD80 pKa = 3.36 LTEE83 pKa = 4.54 FGGSLIDD90 pKa = 3.52 EE91 pKa = 4.53 NLNIEE96 pKa = 4.26 VTFFATGMLDD106 pKa = 3.09 SSTGGPSAYY115 pKa = 9.75 EE116 pKa = 3.68 DD117 pKa = 3.45 LAFYY121 pKa = 10.84 DD122 pKa = 3.94 VTQASIDD129 pKa = 3.88 FLLGASSTTTLTQVFSDD146 pKa = 4.86 LEE148 pKa = 4.31 TMGAGGSSFGTKK160 pKa = 10.06 RR161 pKa = 11.84 INDD164 pKa = 3.43 SDD166 pKa = 4.12 LQGEE170 pKa = 5.02 AIPTFSIVLSDD181 pKa = 4.86 AALTDD186 pKa = 4.02 LNLAIGNPEE195 pKa = 3.99 LDD197 pKa = 3.41 YY198 pKa = 10.96 WGLGAHH204 pKa = 6.17 LTSTNGRR211 pKa = 11.84 SVQISSLSITDD222 pKa = 3.73 APTSGLRR229 pKa = 11.84 AVPEE233 pKa = 4.26 VSATGALAAFGSLLAMMAFLWEE255 pKa = 4.14 RR256 pKa = 11.84 RR257 pKa = 11.84 RR258 pKa = 11.84 VTAAAVV264 pKa = 3.12
Molecular weight: 27.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A345R7K1|A0A345R7K1_9RHOB TVP38/TMEM64 family membrane protein OS=Sulfitobacter sp. JL08 OX=2070369 GN=C1J05_02590 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 SGRR28 pKa = 11.84 QILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.31
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.047
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4063
0
4063
1260259
40
2132
310.2
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.699 ± 0.046
0.957 ± 0.013
6.305 ± 0.03
5.468 ± 0.033
3.888 ± 0.025
8.364 ± 0.036
2.11 ± 0.019
5.451 ± 0.027
3.478 ± 0.03
9.804 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.856 ± 0.02
2.886 ± 0.019
4.943 ± 0.026
3.415 ± 0.021
6.309 ± 0.033
5.422 ± 0.026
5.581 ± 0.024
7.262 ± 0.032
1.422 ± 0.016
2.382 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here