Streptococcus phage JX01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7KDL2|J7KDL2_9CAUD Uncharacterized protein OS=Streptococcus phage JX01 OX=1225806 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.41VYY4 pKa = 10.32ILEE7 pKa = 4.01QWTYY11 pKa = 11.29DD12 pKa = 3.56YY13 pKa = 11.21GVSHH17 pKa = 7.11SDD19 pKa = 2.83ILGVYY24 pKa = 10.23SSLEE28 pKa = 3.79KK29 pKa = 10.7ARR31 pKa = 11.84EE32 pKa = 4.23FIDD35 pKa = 3.28NAGYY39 pKa = 10.13HH40 pKa = 5.63YY41 pKa = 10.75EE42 pKa = 4.56DD43 pKa = 5.87GIFSDD48 pKa = 4.91YY49 pKa = 11.45LEE51 pKa = 4.83TYY53 pKa = 10.01CKK55 pKa = 10.28EE56 pKa = 3.43EE57 pKa = 3.82SGIYY61 pKa = 8.44KK62 pKa = 9.33TISIIEE68 pKa = 4.26RR69 pKa = 11.84EE70 pKa = 4.05LDD72 pKa = 3.31SDD74 pKa = 3.64LGEE77 pKa = 4.2WEE79 pKa = 4.36FEE81 pKa = 4.34AVGTEE86 pKa = 3.87

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7KK69|J7KK69_9CAUD Uncharacterized protein OS=Streptococcus phage JX01 OX=1225806 PE=4 SV=1
MM1 pKa = 7.64NEE3 pKa = 4.13QNNKK7 pKa = 7.55LHH9 pKa = 6.23KK10 pKa = 8.86QSIVIVVLTVAVSVLLTTNIALYY33 pKa = 10.41NYY35 pKa = 7.02YY36 pKa = 10.15QPQITGLQQQLIRR49 pKa = 11.84TQYY52 pKa = 9.42RR53 pKa = 11.84LKK55 pKa = 10.67EE56 pKa = 4.09SSEE59 pKa = 3.77QNQRR63 pKa = 11.84QTKK66 pKa = 10.05RR67 pKa = 11.84IAEE70 pKa = 4.08LTGNGGG76 pKa = 3.26

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12831

37

1313

183.3

20.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.36 ± 0.56

0.616 ± 0.11

6.336 ± 0.259

6.874 ± 0.341

4.419 ± 0.297

6.406 ± 0.358

1.294 ± 0.154

7.014 ± 0.334

8.822 ± 0.449

8.363 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.19

6.134 ± 0.203

2.728 ± 0.178

3.694 ± 0.259

3.819 ± 0.236

6.469 ± 0.36

6.344 ± 0.323

6.321 ± 0.252

1.309 ± 0.095

4.294 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski