Salmonella phage SE2
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H2EQG9|H2EQG9_9CAUD Uncharacterized protein OS=Salmonella phage SE2 OX=1115478 PE=4 SV=1
MM1 pKa = 8.57 VEE3 pKa = 3.9 EE4 pKa = 5.34 HH5 pKa = 7.17 YY6 pKa = 9.79 DD7 pKa = 3.14 TAYY10 pKa = 8.92 ITDD13 pKa = 4.16 TSPHH17 pKa = 5.31 MRR19 pKa = 11.84 AFLYY23 pKa = 10.2 TGLSFPIQSLIDD35 pKa = 3.59 AQGVSDD41 pKa = 4.62 ALL43 pKa = 3.76
Molecular weight: 4.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.987
IPC2_protein 4.291
IPC_protein 4.05
Toseland 3.872
ProMoST 4.253
Dawson 4.062
Bjellqvist 4.228
Wikipedia 4.037
Rodwell 3.897
Grimsley 3.795
Solomon 4.037
Lehninger 3.986
Nozaki 4.215
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.037
Sillero 4.19
Patrickios 0.604
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.08
Protein with the highest isoelectric point:
>tr|H2EQJ0|H2EQJ0_9CAUD DNA methylase N-4/N-6 domain protein OS=Salmonella phage SE2 OX=1115478 PE=4 SV=1
MM1 pKa = 8.07 LYY3 pKa = 10.22 PEE5 pKa = 3.74 IRR7 pKa = 11.84 INVFSLSKK15 pKa = 10.73 RR16 pKa = 11.84 NTLLGLPPFEE26 pKa = 5.04 GKK28 pKa = 8.24 TFSSKK33 pKa = 10.39 LRR35 pKa = 11.84 EE36 pKa = 4.02 SSCAIFNISRR46 pKa = 11.84 RR47 pKa = 11.84 TLSCSFPVNSAISRR61 pKa = 11.84 LDD63 pKa = 3.4 MVCASLTFINSFMVSSPLVGVDD85 pKa = 2.62 IFNFEE90 pKa = 3.33 HH91 pKa = 7.17 SYY93 pKa = 10.84 RR94 pKa = 11.84 SNQILYY100 pKa = 10.21 RR101 pKa = 11.84 DD102 pKa = 4.15 DD103 pKa = 3.36 KK104 pKa = 11.06 KK105 pKa = 11.0 KK106 pKa = 10.62 RR107 pKa = 11.84 PRR109 pKa = 11.84 KK110 pKa = 10.01 GPFSHH115 pKa = 7.17 SSILLRR121 pKa = 11.84 YY122 pKa = 8.38 FRR124 pKa = 11.84 NFSRR128 pKa = 11.84 LSIASAKK135 pKa = 9.74 SVPVSLCPRR144 pKa = 11.84 KK145 pKa = 9.48 PIHH148 pKa = 6.52 NSFFNACCSAGSRR161 pKa = 11.84 ISNALRR167 pKa = 11.84 TSYY170 pKa = 10.25 TSQVMLSPSVFVIVRR185 pKa = 11.84 LYY187 pKa = 10.93 LFVIFMPYY195 pKa = 10.13 LL196 pKa = 3.7
Molecular weight: 22.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.409
Toseland 10.438
ProMoST 10.16
Dawson 10.599
Bjellqvist 10.335
Wikipedia 10.804
Rodwell 10.877
Grimsley 10.672
Solomon 10.672
Lehninger 10.628
Nozaki 10.482
DTASelect 10.306
Thurlkill 10.482
EMBOSS 10.847
Sillero 10.54
Patrickios 10.555
IPC_peptide 10.672
IPC2_peptide 9.531
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
12602
43
852
206.6
22.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.522 ± 0.604
1.008 ± 0.14
5.92 ± 0.211
6.681 ± 0.448
3.841 ± 0.206
7.665 ± 0.35
1.651 ± 0.19
4.92 ± 0.229
5.959 ± 0.347
7.673 ± 0.312
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.674 ± 0.142
4.483 ± 0.25
3.991 ± 0.227
3.777 ± 0.234
5.666 ± 0.209
6.007 ± 0.312
6.499 ± 0.282
7.237 ± 0.3
1.381 ± 0.14
3.444 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here