Salmonella phage SE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; Salmonella virus SE2

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H2EQG9|H2EQG9_9CAUD Uncharacterized protein OS=Salmonella phage SE2 OX=1115478 PE=4 SV=1
MM1 pKa = 8.57VEE3 pKa = 3.9EE4 pKa = 5.34HH5 pKa = 7.17YY6 pKa = 9.79DD7 pKa = 3.14TAYY10 pKa = 8.92ITDD13 pKa = 4.16TSPHH17 pKa = 5.31MRR19 pKa = 11.84AFLYY23 pKa = 10.2TGLSFPIQSLIDD35 pKa = 3.59AQGVSDD41 pKa = 4.62ALL43 pKa = 3.76

Molecular weight:
4.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H2EQJ0|H2EQJ0_9CAUD DNA methylase N-4/N-6 domain protein OS=Salmonella phage SE2 OX=1115478 PE=4 SV=1
MM1 pKa = 8.07LYY3 pKa = 10.22PEE5 pKa = 3.74IRR7 pKa = 11.84INVFSLSKK15 pKa = 10.73RR16 pKa = 11.84NTLLGLPPFEE26 pKa = 5.04GKK28 pKa = 8.24TFSSKK33 pKa = 10.39LRR35 pKa = 11.84EE36 pKa = 4.02SSCAIFNISRR46 pKa = 11.84RR47 pKa = 11.84TLSCSFPVNSAISRR61 pKa = 11.84LDD63 pKa = 3.4MVCASLTFINSFMVSSPLVGVDD85 pKa = 2.62IFNFEE90 pKa = 3.33HH91 pKa = 7.17SYY93 pKa = 10.84RR94 pKa = 11.84SNQILYY100 pKa = 10.21RR101 pKa = 11.84DD102 pKa = 4.15DD103 pKa = 3.36KK104 pKa = 11.06KK105 pKa = 11.0KK106 pKa = 10.62RR107 pKa = 11.84PRR109 pKa = 11.84KK110 pKa = 10.01GPFSHH115 pKa = 7.17SSILLRR121 pKa = 11.84YY122 pKa = 8.38FRR124 pKa = 11.84NFSRR128 pKa = 11.84LSIASAKK135 pKa = 9.74SVPVSLCPRR144 pKa = 11.84KK145 pKa = 9.48PIHH148 pKa = 6.52NSFFNACCSAGSRR161 pKa = 11.84ISNALRR167 pKa = 11.84TSYY170 pKa = 10.25TSQVMLSPSVFVIVRR185 pKa = 11.84LYY187 pKa = 10.93LFVIFMPYY195 pKa = 10.13LL196 pKa = 3.7

Molecular weight:
22.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12602

43

852

206.6

22.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.522 ± 0.604

1.008 ± 0.14

5.92 ± 0.211

6.681 ± 0.448

3.841 ± 0.206

7.665 ± 0.35

1.651 ± 0.19

4.92 ± 0.229

5.959 ± 0.347

7.673 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.142

4.483 ± 0.25

3.991 ± 0.227

3.777 ± 0.234

5.666 ± 0.209

6.007 ± 0.312

6.499 ± 0.282

7.237 ± 0.3

1.381 ± 0.14

3.444 ± 0.163

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski