Amino acid dipepetide frequency for Salmonella phage SE2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.19AlaAla: 11.19 ± 1.632
1.27AlaCys: 1.27 ± 0.297
5.873AlaAsp: 5.873 ± 0.778
6.111AlaGlu: 6.111 ± 0.801
3.73AlaPhe: 3.73 ± 0.49
7.618AlaGly: 7.618 ± 0.772
2.063AlaHis: 2.063 ± 0.469
4.127AlaIle: 4.127 ± 0.676
5.634AlaLys: 5.634 ± 0.846
7.936AlaLeu: 7.936 ± 1.1
2.698AlaMet: 2.698 ± 0.481
3.412AlaAsn: 3.412 ± 0.428
3.333AlaPro: 3.333 ± 0.407
3.333AlaGln: 3.333 ± 0.587
4.206AlaArg: 4.206 ± 0.645
5.873AlaSer: 5.873 ± 0.92
5.634AlaThr: 5.634 ± 0.643
6.269AlaVal: 6.269 ± 0.737
1.19AlaTrp: 1.19 ± 0.275
3.651AlaTyr: 3.651 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.794CysAla: 0.794 ± 0.294
0.159CysCys: 0.159 ± 0.111
0.714CysAsp: 0.714 ± 0.225
1.27CysGlu: 1.27 ± 0.347
0.397CysPhe: 0.397 ± 0.165
0.794CysGly: 0.794 ± 0.258
0.159CysHis: 0.159 ± 0.113
0.238CysIle: 0.238 ± 0.118
0.794CysLys: 0.794 ± 0.251
0.873CysLeu: 0.873 ± 0.29
0.238CysMet: 0.238 ± 0.158
0.317CysAsn: 0.317 ± 0.185
0.317CysPro: 0.317 ± 0.155
0.159CysGln: 0.159 ± 0.12
0.635CysArg: 0.635 ± 0.249
0.476CysSer: 0.476 ± 0.223
0.556CysThr: 0.556 ± 0.186
0.714CysVal: 0.714 ± 0.241
0.238CysTrp: 0.238 ± 0.139
0.238CysTyr: 0.238 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
6.269AspAla: 6.269 ± 0.808
0.794AspCys: 0.794 ± 0.251
3.571AspAsp: 3.571 ± 0.507
3.809AspGlu: 3.809 ± 0.611
2.698AspPhe: 2.698 ± 0.461
6.031AspGly: 6.031 ± 0.827
0.794AspHis: 0.794 ± 0.262
3.174AspIle: 3.174 ± 0.369
3.095AspLys: 3.095 ± 0.351
4.92AspLeu: 4.92 ± 0.578
1.508AspMet: 1.508 ± 0.254
2.936AspAsn: 2.936 ± 0.568
1.825AspPro: 1.825 ± 0.368
0.714AspGln: 0.714 ± 0.219
2.698AspArg: 2.698 ± 0.446
3.571AspSer: 3.571 ± 0.528
4.841AspThr: 4.841 ± 0.565
3.492AspVal: 3.492 ± 0.394
0.794AspTrp: 0.794 ± 0.222
1.667AspTyr: 1.667 ± 0.352
0.0AspXaa: 0.0 ± 0.0
Glu
5.952GluAla: 5.952 ± 0.969
0.397GluCys: 0.397 ± 0.17
3.651GluAsp: 3.651 ± 0.528
5.158GluGlu: 5.158 ± 1.028
3.571GluPhe: 3.571 ± 0.768
4.682GluGly: 4.682 ± 0.792
1.032GluHis: 1.032 ± 0.277
3.651GluIle: 3.651 ± 0.49
3.73GluLys: 3.73 ± 0.637
5.476GluLeu: 5.476 ± 0.676
2.778GluMet: 2.778 ± 0.479
2.381GluAsn: 2.381 ± 0.416
1.825GluPro: 1.825 ± 0.483
3.095GluGln: 3.095 ± 0.6
3.809GluArg: 3.809 ± 0.608
3.809GluSer: 3.809 ± 0.519
4.047GluThr: 4.047 ± 0.519
4.841GluVal: 4.841 ± 0.634
0.952GluTrp: 0.952 ± 0.284
1.984GluTyr: 1.984 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
3.016PheAla: 3.016 ± 0.594
0.317PheCys: 0.317 ± 0.155
3.016PheAsp: 3.016 ± 0.487
3.174PheGlu: 3.174 ± 0.491
0.794PhePhe: 0.794 ± 0.242
2.857PheGly: 2.857 ± 0.405
0.556PheHis: 0.556 ± 0.208
2.46PheIle: 2.46 ± 0.403
1.667PheLys: 1.667 ± 0.417
2.301PheLeu: 2.301 ± 0.427
0.794PheMet: 0.794 ± 0.256
1.746PheAsn: 1.746 ± 0.387
1.746PhePro: 1.746 ± 0.456
1.428PheGln: 1.428 ± 0.334
2.222PheArg: 2.222 ± 0.328
2.619PheSer: 2.619 ± 0.56
3.412PheThr: 3.412 ± 0.562
2.381PheVal: 2.381 ± 0.471
0.794PheTrp: 0.794 ± 0.303
1.111PheTyr: 1.111 ± 0.332
0.0PheXaa: 0.0 ± 0.0
Gly
7.063GlyAla: 7.063 ± 0.85
0.794GlyCys: 0.794 ± 0.294
3.889GlyAsp: 3.889 ± 0.598
5.793GlyGlu: 5.793 ± 0.995
2.778GlyPhe: 2.778 ± 0.608
6.745GlyGly: 6.745 ± 0.818
1.27GlyHis: 1.27 ± 0.476
3.492GlyIle: 3.492 ± 0.534
5.396GlyLys: 5.396 ± 0.602
5.396GlyLeu: 5.396 ± 0.58
2.301GlyMet: 2.301 ± 0.625
4.047GlyAsn: 4.047 ± 0.629
1.825GlyPro: 1.825 ± 0.394
3.016GlyGln: 3.016 ± 0.499
4.682GlyArg: 4.682 ± 0.599
5.0GlySer: 5.0 ± 0.821
3.73GlyThr: 3.73 ± 0.648
5.873GlyVal: 5.873 ± 0.567
0.873GlyTrp: 0.873 ± 0.233
2.698GlyTyr: 2.698 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
1.19HisAla: 1.19 ± 0.331
0.397HisCys: 0.397 ± 0.169
0.794HisAsp: 0.794 ± 0.179
0.952HisGlu: 0.952 ± 0.282
0.714HisPhe: 0.714 ± 0.298
0.635HisGly: 0.635 ± 0.265
0.794HisHis: 0.794 ± 0.297
0.873HisIle: 0.873 ± 0.252
1.428HisLys: 1.428 ± 0.3
1.428HisLeu: 1.428 ± 0.393
0.556HisMet: 0.556 ± 0.213
0.556HisAsn: 0.556 ± 0.25
1.032HisPro: 1.032 ± 0.265
0.873HisGln: 0.873 ± 0.212
0.873HisArg: 0.873 ± 0.265
0.873HisSer: 0.873 ± 0.257
0.794HisThr: 0.794 ± 0.346
0.714HisVal: 0.714 ± 0.199
0.079HisTrp: 0.079 ± 0.091
0.952HisTyr: 0.952 ± 0.374
0.0HisXaa: 0.0 ± 0.0
Ile
4.603IleAla: 4.603 ± 0.709
0.556IleCys: 0.556 ± 0.234
3.889IleAsp: 3.889 ± 0.659
2.936IleGlu: 2.936 ± 0.503
1.508IlePhe: 1.508 ± 0.408
3.254IleGly: 3.254 ± 0.476
0.635IleHis: 0.635 ± 0.204
2.301IleIle: 2.301 ± 0.488
2.857IleLys: 2.857 ± 0.494
3.492IleLeu: 3.492 ± 0.499
1.19IleMet: 1.19 ± 0.334
2.619IleAsn: 2.619 ± 0.505
2.857IlePro: 2.857 ± 0.478
1.746IleGln: 1.746 ± 0.435
2.381IleArg: 2.381 ± 0.331
2.936IleSer: 2.936 ± 0.528
4.365IleThr: 4.365 ± 0.597
3.254IleVal: 3.254 ± 0.4
0.635IleTrp: 0.635 ± 0.206
1.19IleTyr: 1.19 ± 0.368
0.0IleXaa: 0.0 ± 0.0
Lys
5.793LysAla: 5.793 ± 0.776
0.476LysCys: 0.476 ± 0.171
3.412LysAsp: 3.412 ± 0.542
4.047LysGlu: 4.047 ± 0.57
1.984LysPhe: 1.984 ± 0.295
3.889LysGly: 3.889 ± 0.44
0.952LysHis: 0.952 ± 0.253
1.746LysIle: 1.746 ± 0.432
3.333LysLys: 3.333 ± 0.669
5.158LysLeu: 5.158 ± 0.564
2.698LysMet: 2.698 ± 0.533
2.698LysAsn: 2.698 ± 0.48
2.778LysPro: 2.778 ± 0.546
2.698LysGln: 2.698 ± 0.512
3.809LysArg: 3.809 ± 0.583
2.778LysSer: 2.778 ± 0.457
4.285LysThr: 4.285 ± 0.529
3.73LysVal: 3.73 ± 0.572
0.714LysTrp: 0.714 ± 0.257
2.619LysTyr: 2.619 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
7.142LeuAla: 7.142 ± 0.669
0.714LeuCys: 0.714 ± 0.26
3.968LeuAsp: 3.968 ± 0.541
4.682LeuGlu: 4.682 ± 0.963
1.587LeuPhe: 1.587 ± 0.372
4.841LeuGly: 4.841 ± 0.612
1.428LeuHis: 1.428 ± 0.405
4.285LeuIle: 4.285 ± 0.462
5.555LeuLys: 5.555 ± 0.727
5.714LeuLeu: 5.714 ± 0.718
1.984LeuMet: 1.984 ± 0.326
4.444LeuAsn: 4.444 ± 0.594
3.333LeuPro: 3.333 ± 0.575
2.619LeuGln: 2.619 ± 0.445
5.238LeuArg: 5.238 ± 0.671
4.523LeuSer: 4.523 ± 0.491
5.317LeuThr: 5.317 ± 0.472
5.396LeuVal: 5.396 ± 0.46
1.27LeuTrp: 1.27 ± 0.334
2.698LeuTyr: 2.698 ± 0.438
0.0LeuXaa: 0.0 ± 0.0
Met
2.539MetAla: 2.539 ± 0.393
0.238MetCys: 0.238 ± 0.162
1.27MetAsp: 1.27 ± 0.388
1.349MetGlu: 1.349 ± 0.343
1.111MetPhe: 1.111 ± 0.351
2.063MetGly: 2.063 ± 0.441
0.397MetHis: 0.397 ± 0.144
1.27MetIle: 1.27 ± 0.31
1.349MetLys: 1.349 ± 0.311
2.063MetLeu: 2.063 ± 0.397
0.873MetMet: 0.873 ± 0.299
1.111MetAsn: 1.111 ± 0.315
1.19MetPro: 1.19 ± 0.396
0.952MetGln: 0.952 ± 0.239
1.587MetArg: 1.587 ± 0.329
1.984MetSer: 1.984 ± 0.317
1.905MetThr: 1.905 ± 0.325
2.143MetVal: 2.143 ± 0.377
0.476MetTrp: 0.476 ± 0.177
0.873MetTyr: 0.873 ± 0.256
0.0MetXaa: 0.0 ± 0.0
Asn
4.127AsnAla: 4.127 ± 0.495
0.476AsnCys: 0.476 ± 0.174
2.857AsnAsp: 2.857 ± 0.43
2.222AsnGlu: 2.222 ± 0.378
2.063AsnPhe: 2.063 ± 0.379
3.889AsnGly: 3.889 ± 0.546
0.317AsnHis: 0.317 ± 0.13
2.857AsnIle: 2.857 ± 0.399
2.222AsnLys: 2.222 ± 0.516
3.73AsnLeu: 3.73 ± 0.447
0.556AsnMet: 0.556 ± 0.218
2.301AsnAsn: 2.301 ± 0.439
1.905AsnPro: 1.905 ± 0.487
1.508AsnGln: 1.508 ± 0.343
2.857AsnArg: 2.857 ± 0.437
2.936AsnSer: 2.936 ± 0.426
2.063AsnThr: 2.063 ± 0.41
3.73AsnVal: 3.73 ± 0.452
0.794AsnTrp: 0.794 ± 0.218
1.428AsnTyr: 1.428 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
2.778ProAla: 2.778 ± 0.549
0.317ProCys: 0.317 ± 0.169
2.698ProAsp: 2.698 ± 0.512
3.492ProGlu: 3.492 ± 0.479
1.746ProPhe: 1.746 ± 0.359
3.095ProGly: 3.095 ± 0.5
0.635ProHis: 0.635 ± 0.171
1.746ProIle: 1.746 ± 0.376
2.778ProLys: 2.778 ± 0.527
3.492ProLeu: 3.492 ± 0.522
1.111ProMet: 1.111 ± 0.311
1.587ProAsn: 1.587 ± 0.471
1.428ProPro: 1.428 ± 0.408
1.19ProGln: 1.19 ± 0.277
2.301ProArg: 2.301 ± 0.478
1.825ProSer: 1.825 ± 0.322
1.825ProThr: 1.825 ± 0.343
3.968ProVal: 3.968 ± 0.563
0.317ProTrp: 0.317 ± 0.199
1.587ProTyr: 1.587 ± 0.408
0.0ProXaa: 0.0 ± 0.0
Gln
4.444GlnAla: 4.444 ± 0.684
0.317GlnCys: 0.317 ± 0.142
1.508GlnAsp: 1.508 ± 0.329
2.301GlnGlu: 2.301 ± 0.52
1.428GlnPhe: 1.428 ± 0.451
2.063GlnGly: 2.063 ± 0.482
0.476GlnHis: 0.476 ± 0.203
1.984GlnIle: 1.984 ± 0.418
2.063GlnLys: 2.063 ± 0.471
2.936GlnLeu: 2.936 ± 0.567
1.27GlnMet: 1.27 ± 0.358
1.984GlnAsn: 1.984 ± 0.391
2.143GlnPro: 2.143 ± 0.328
2.301GlnGln: 2.301 ± 0.511
1.667GlnArg: 1.667 ± 0.317
2.063GlnSer: 2.063 ± 0.309
1.984GlnThr: 1.984 ± 0.336
2.778GlnVal: 2.778 ± 0.438
0.714GlnTrp: 0.714 ± 0.227
1.349GlnTyr: 1.349 ± 0.301
0.0GlnXaa: 0.0 ± 0.0
Arg
4.682ArgAla: 4.682 ± 0.493
0.397ArgCys: 0.397 ± 0.123
3.651ArgAsp: 3.651 ± 0.526
3.651ArgGlu: 3.651 ± 0.524
1.746ArgPhe: 1.746 ± 0.395
3.809ArgGly: 3.809 ± 0.585
1.19ArgHis: 1.19 ± 0.349
3.333ArgIle: 3.333 ± 0.54
3.809ArgLys: 3.809 ± 0.678
4.047ArgLeu: 4.047 ± 0.52
1.746ArgMet: 1.746 ± 0.313
3.254ArgAsn: 3.254 ± 0.545
2.143ArgPro: 2.143 ± 0.366
3.174ArgGln: 3.174 ± 0.508
4.285ArgArg: 4.285 ± 0.685
1.905ArgSer: 1.905 ± 0.292
2.857ArgThr: 2.857 ± 0.414
4.285ArgVal: 4.285 ± 0.527
1.032ArgTrp: 1.032 ± 0.307
1.667ArgTyr: 1.667 ± 0.431
0.0ArgXaa: 0.0 ± 0.0
Ser
6.825SerAla: 6.825 ± 1.063
0.397SerCys: 0.397 ± 0.21
3.016SerAsp: 3.016 ± 0.472
3.095SerGlu: 3.095 ± 0.595
2.698SerPhe: 2.698 ± 0.42
6.745SerGly: 6.745 ± 0.802
0.873SerHis: 0.873 ± 0.247
2.539SerIle: 2.539 ± 0.397
2.381SerLys: 2.381 ± 0.39
4.841SerLeu: 4.841 ± 0.603
1.032SerMet: 1.032 ± 0.318
2.539SerAsn: 2.539 ± 0.341
1.984SerPro: 1.984 ± 0.447
2.222SerGln: 2.222 ± 0.337
3.412SerArg: 3.412 ± 0.522
3.492SerSer: 3.492 ± 0.547
3.968SerThr: 3.968 ± 0.604
5.396SerVal: 5.396 ± 0.831
0.873SerTrp: 0.873 ± 0.208
1.746SerTyr: 1.746 ± 0.378
0.0SerXaa: 0.0 ± 0.0
Thr
6.428ThrAla: 6.428 ± 0.763
0.238ThrCys: 0.238 ± 0.139
4.365ThrAsp: 4.365 ± 0.555
3.412ThrGlu: 3.412 ± 0.489
3.095ThrPhe: 3.095 ± 0.49
5.952ThrGly: 5.952 ± 0.782
0.714ThrHis: 0.714 ± 0.286
2.857ThrIle: 2.857 ± 0.482
3.095ThrLys: 3.095 ± 0.44
5.238ThrLeu: 5.238 ± 0.794
0.952ThrMet: 0.952 ± 0.37
1.905ThrAsn: 1.905 ± 0.38
3.889ThrPro: 3.889 ± 0.53
2.063ThrGln: 2.063 ± 0.338
3.254ThrArg: 3.254 ± 0.449
5.238ThrSer: 5.238 ± 0.655
3.809ThrThr: 3.809 ± 0.584
4.762ThrVal: 4.762 ± 0.784
1.032ThrTrp: 1.032 ± 0.266
2.698ThrTyr: 2.698 ± 0.506
0.0ThrXaa: 0.0 ± 0.0
Val
6.428ValAla: 6.428 ± 0.826
1.032ValCys: 1.032 ± 0.33
4.047ValAsp: 4.047 ± 0.499
6.587ValGlu: 6.587 ± 0.69
2.301ValPhe: 2.301 ± 0.525
3.73ValGly: 3.73 ± 0.53
1.111ValHis: 1.111 ± 0.264
4.206ValIle: 4.206 ± 0.628
5.079ValLys: 5.079 ± 0.644
4.206ValLeu: 4.206 ± 0.629
1.111ValMet: 1.111 ± 0.223
3.016ValAsn: 3.016 ± 0.604
2.539ValPro: 2.539 ± 0.647
2.301ValGln: 2.301 ± 0.364
3.412ValArg: 3.412 ± 0.473
6.031ValSer: 6.031 ± 0.789
6.428ValThr: 6.428 ± 0.772
5.317ValVal: 5.317 ± 0.788
0.794ValTrp: 0.794 ± 0.263
2.698ValTyr: 2.698 ± 0.495
0.0ValXaa: 0.0 ± 0.0
Trp
1.032TrpAla: 1.032 ± 0.266
0.159TrpCys: 0.159 ± 0.112
0.952TrpAsp: 0.952 ± 0.284
0.397TrpGlu: 0.397 ± 0.165
1.032TrpPhe: 1.032 ± 0.338
0.952TrpGly: 0.952 ± 0.263
0.317TrpHis: 0.317 ± 0.207
0.476TrpIle: 0.476 ± 0.192
0.714TrpLys: 0.714 ± 0.286
1.428TrpLeu: 1.428 ± 0.29
0.476TrpMet: 0.476 ± 0.202
0.714TrpAsn: 0.714 ± 0.197
0.476TrpPro: 0.476 ± 0.202
0.635TrpGln: 0.635 ± 0.216
1.032TrpArg: 1.032 ± 0.404
0.476TrpSer: 0.476 ± 0.178
0.794TrpThr: 0.794 ± 0.247
1.27TrpVal: 1.27 ± 0.267
0.238TrpTrp: 0.238 ± 0.152
0.238TrpTyr: 0.238 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.936TyrAla: 2.936 ± 0.5
0.635TyrCys: 0.635 ± 0.295
2.063TyrAsp: 2.063 ± 0.53
2.381TyrGlu: 2.381 ± 0.531
1.428TyrPhe: 1.428 ± 0.374
3.016TyrGly: 3.016 ± 0.424
0.794TyrHis: 0.794 ± 0.236
1.587TyrIle: 1.587 ± 0.391
2.619TyrLys: 2.619 ± 0.533
2.063TyrLeu: 2.063 ± 0.386
0.794TyrMet: 0.794 ± 0.198
1.27TyrAsn: 1.27 ± 0.317
1.349TyrPro: 1.349 ± 0.422
1.746TyrGln: 1.746 ± 0.313
2.301TyrArg: 2.301 ± 0.461
1.667TyrSer: 1.667 ± 0.436
2.381TyrThr: 2.381 ± 0.408
2.063TyrVal: 2.063 ± 0.393
0.0TyrTrp: 0.0 ± 0.0
1.349TyrTyr: 1.349 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (12602 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski