Oleispira antarctica RB-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Oleispira; Oleispira antarctica

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3895 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4YPX3|R4YPX3_OLEAN Ethanolamine utilisation protein EutH OS=Oleispira antarctica RB-8 OX=698738 GN=OLEAN_C11370 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.49SSTPSMKK9 pKa = 10.48LGLASAIAVAALGMTGCGGDD29 pKa = 4.21DD30 pKa = 3.37SSSSSKK36 pKa = 9.55STSNLIDD43 pKa = 3.53PCLNADD49 pKa = 3.78CTNSGFILPSNAIYY63 pKa = 10.78VSADD67 pKa = 3.19AADD70 pKa = 4.07GTDD73 pKa = 4.27LKK75 pKa = 11.15NALITALTDD84 pKa = 3.19IPDD87 pKa = 3.42NSVIVLPKK95 pKa = 10.21GSFIVSSSITVTDD108 pKa = 3.36ATGITITGHH117 pKa = 6.08GMTEE121 pKa = 4.05TKK123 pKa = 10.54LDD125 pKa = 3.99FSSAPDD131 pKa = 3.59DD132 pKa = 5.17DD133 pKa = 4.18GFKK136 pKa = 10.62FAGGTDD142 pKa = 3.01ITIRR146 pKa = 11.84DD147 pKa = 3.87LGVYY151 pKa = 7.59EE152 pKa = 4.33APKK155 pKa = 10.54NAIKK159 pKa = 10.67ADD161 pKa = 3.81GVNGIHH167 pKa = 6.11ITYY170 pKa = 9.47TSAVWEE176 pKa = 4.25TDD178 pKa = 3.3LEE180 pKa = 4.41EE181 pKa = 5.9GGGNNGAYY189 pKa = 10.13GLYY192 pKa = 9.57PVSSQNVLMEE202 pKa = 4.33NNYY205 pKa = 10.86SKK207 pKa = 11.29GSADD211 pKa = 3.0AGIYY215 pKa = 9.7VGQSNNIVVRR225 pKa = 11.84NNIAEE230 pKa = 4.3HH231 pKa = 5.59NVAGIEE237 pKa = 4.06IEE239 pKa = 4.19NSNKK243 pKa = 9.85ADD245 pKa = 3.39VYY247 pKa = 11.61NNVAFDD253 pKa = 3.64NSAGILSFDD262 pKa = 5.3LPNLPQGYY270 pKa = 9.41GGGVRR275 pKa = 11.84IFNNNTFDD283 pKa = 4.24NNTTNVGAGAVSLAPSGTGILIFATSDD310 pKa = 3.25VEE312 pKa = 4.52IYY314 pKa = 11.3NNTIADD320 pKa = 3.75NDD322 pKa = 4.09TAGIEE327 pKa = 3.91IASYY331 pKa = 10.39FLADD335 pKa = 4.83DD336 pKa = 4.64DD337 pKa = 4.27VANYY341 pKa = 6.68GTNYY345 pKa = 9.1GATMANGWSPLIKK358 pKa = 10.46NINIHH363 pKa = 7.48DD364 pKa = 3.98NTFKK368 pKa = 11.11DD369 pKa = 3.54NSLASPPKK377 pKa = 9.69TGLLEE382 pKa = 5.15DD383 pKa = 5.06IIAGYY388 pKa = 10.28QSAFNSTGSAQTAPAIIYY406 pKa = 9.96GGIGEE411 pKa = 4.74LLSNAGQLTGFDD423 pKa = 3.69ALVGAEE429 pKa = 3.97AKK431 pKa = 10.62ADD433 pKa = 3.68GVDD436 pKa = 3.38YY437 pKa = 10.87DD438 pKa = 4.67AYY440 pKa = 10.81AAGDD444 pKa = 4.54LICSHH449 pKa = 6.88SNINNNNKK457 pKa = 8.19NTLNSNDD464 pKa = 3.75LNVGLVYY471 pKa = 9.6GTDD474 pKa = 3.45ATDD477 pKa = 3.47TNNWNAAGTAPEE489 pKa = 3.81ATLRR493 pKa = 11.84IDD495 pKa = 5.15LIEE498 pKa = 4.61NNTLLDD504 pKa = 3.89CTLEE508 pKa = 4.12RR509 pKa = 11.84LPPAVVTIKK518 pKa = 9.46GTKK521 pKa = 8.95YY522 pKa = 10.7GCTADD527 pKa = 4.35DD528 pKa = 4.99LNLAACSLL536 pKa = 4.0

Molecular weight: 55.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4YSX9|R4YSX9_OLEAN Uncharacterized protein OS=Oleispira antarctica RB-8 OX=698738 GN=OLEAN_C11680 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.12SGRR28 pKa = 11.84QIISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.59GRR39 pKa = 11.84TKK41 pKa = 10.86LSAA44 pKa = 3.61

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3895

0

3895

1295253

38

4838

332.5

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.508 ± 0.047

0.978 ± 0.013

5.783 ± 0.039

6.326 ± 0.035

4.144 ± 0.028

6.678 ± 0.043

2.189 ± 0.02

6.86 ± 0.033

5.563 ± 0.036

10.4 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.021

4.578 ± 0.037

3.736 ± 0.029

4.571 ± 0.033

4.395 ± 0.031

6.882 ± 0.04

5.251 ± 0.038

6.387 ± 0.035

1.243 ± 0.016

2.985 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski