Simian adenovirus 6
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9TER0|H9TER0_9ADEN E1B protein small T-antigen OS=Simian adenovirus 6 OX=413259 PE=3 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 NFLLSPGLPATVAAEE17 pKa = 3.97 LLEE20 pKa = 5.42 DD21 pKa = 3.79 IVTGALGDD29 pKa = 3.93 DD30 pKa = 3.97 PQVISHH36 pKa = 6.59 FCEE39 pKa = 4.58 DD40 pKa = 4.2 FSLHH44 pKa = 6.83 DD45 pKa = 5.55 LYY47 pKa = 11.28 DD48 pKa = 4.02 IDD50 pKa = 5.19 PGVEE54 pKa = 4.03 GQEE57 pKa = 4.33 DD58 pKa = 3.75 EE59 pKa = 4.35 WLEE62 pKa = 4.13 SVDD65 pKa = 5.06 GFFPDD70 pKa = 4.93 AMLLEE75 pKa = 5.16 ADD77 pKa = 5.18 LPPPHH82 pKa = 6.87 NSHH85 pKa = 6.76 TEE87 pKa = 4.0 PEE89 pKa = 4.32 SAAIPEE95 pKa = 4.35 LSSGEE100 pKa = 4.27 LDD102 pKa = 3.61 LACYY106 pKa = 7.85 EE107 pKa = 4.29 TMPPEE112 pKa = 4.3 SDD114 pKa = 4.0 EE115 pKa = 4.24 EE116 pKa = 4.34 DD117 pKa = 3.58 SGISDD122 pKa = 3.51 PTAFMVSKK130 pKa = 10.74 AIAILKK136 pKa = 10.1 EE137 pKa = 4.5 DD138 pKa = 4.84 DD139 pKa = 4.9 DD140 pKa = 6.75 GDD142 pKa = 4.07 DD143 pKa = 4.37 GFRR146 pKa = 11.84 LDD148 pKa = 4.08 APAVPGRR155 pKa = 11.84 DD156 pKa = 3.41 CKK158 pKa = 10.43 SCEE161 pKa = 3.74 YY162 pKa = 10.74 HH163 pKa = 7.38 RR164 pKa = 11.84 DD165 pKa = 3.52 RR166 pKa = 11.84 TGDD169 pKa = 3.47 PSMLCSLCYY178 pKa = 10.37 LRR180 pKa = 11.84 LNAAFVYY187 pKa = 10.67 SPVSDD192 pKa = 3.8 VEE194 pKa = 4.59 EE195 pKa = 4.72 PDD197 pKa = 3.39 STTGNEE203 pKa = 4.11 EE204 pKa = 4.27 EE205 pKa = 5.01 KK206 pKa = 10.6 PSPPKK211 pKa = 9.52 LTQRR215 pKa = 11.84 CRR217 pKa = 11.84 PNILRR222 pKa = 11.84 PSAQRR227 pKa = 11.84 VSSRR231 pKa = 11.84 KK232 pKa = 8.93 RR233 pKa = 11.84 AAVNCIEE240 pKa = 5.07 DD241 pKa = 4.13 LLEE244 pKa = 4.59 EE245 pKa = 4.21 PTEE248 pKa = 4.11 PLDD251 pKa = 4.98 LSLKK255 pKa = 10.28 RR256 pKa = 11.84 PRR258 pKa = 11.84 PQQ260 pKa = 3.17
Molecular weight: 28.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.212
IPC2_protein 4.101
IPC_protein 4.088
Toseland 3.884
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.948
Rodwell 3.91
Grimsley 3.795
Solomon 4.05
Lehninger 3.999
Nozaki 4.151
DTASelect 4.355
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.19
Patrickios 3.478
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.117
Protein with the highest isoelectric point:
>tr|H9TES3|H9TES3_9ADEN Hexon protein OS=Simian adenovirus 6 OX=413259 GN=L3 PE=2 SV=1
MM1 pKa = 7.65 GKK3 pKa = 8.39 LTCRR7 pKa = 11.84 LRR9 pKa = 11.84 MAVPGYY15 pKa = 9.66 RR16 pKa = 11.84 GRR18 pKa = 11.84 SRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 TLGGSGLRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 AVHH37 pKa = 6.26 RR38 pKa = 11.84 RR39 pKa = 11.84 LKK41 pKa = 10.98 GGILPALIPIIAAAIGAIPGIASVAVQASQRR72 pKa = 11.84 HH73 pKa = 4.3
Molecular weight: 7.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.397
IPC2_protein 10.994
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.164
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
5
32
10956
73
1169
342.4
38.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.041 ± 0.39
1.972 ± 0.305
4.792 ± 0.281
5.969 ± 0.498
4.116 ± 0.374
5.942 ± 0.405
2.391 ± 0.253
4.08 ± 0.28
3.432 ± 0.317
10.013 ± 0.473
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.191 ± 0.226
4.409 ± 0.553
6.982 ± 0.339
4.253 ± 0.212
7.448 ± 0.67
6.8 ± 0.335
5.814 ± 0.305
6.553 ± 0.395
1.342 ± 0.108
3.459 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here