Lactobacillus phage Lrm1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Larmunavirus; Lactobacillus virus Lrm1

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4XYT6|B4XYT6_9CAUD Uncharacterized protein OS=Lactobacillus phage Lrm1 OX=496874 PE=4 SV=1
MM1 pKa = 7.1AWVVRR6 pKa = 11.84GEE8 pKa = 3.82EE9 pKa = 3.9AGIIDD14 pKa = 4.47YY15 pKa = 11.27YY16 pKa = 11.07FDD18 pKa = 3.91NLSKK22 pKa = 10.43RR23 pKa = 11.84HH24 pKa = 5.78KK25 pKa = 10.78AVIGDD30 pKa = 3.97FAVTMGDD37 pKa = 3.15PLYY40 pKa = 10.29RR41 pKa = 11.84FKK43 pKa = 11.38SEE45 pKa = 4.69AEE47 pKa = 4.0AEE49 pKa = 4.34TIAMNVYY56 pKa = 10.64YY57 pKa = 9.82DD58 pKa = 4.41DD59 pKa = 4.29EE60 pKa = 6.31DD61 pKa = 3.73MYY63 pKa = 10.93PYY65 pKa = 10.85EE66 pKa = 4.28VTDD69 pKa = 4.82DD70 pKa = 3.73EE71 pKa = 4.65

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4XYT2|B4XYT2_9CAUD Uncharacterized protein OS=Lactobacillus phage Lrm1 OX=496874 PE=4 SV=1
MM1 pKa = 7.95GIRR4 pKa = 11.84KK5 pKa = 8.99RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 8.67GKK10 pKa = 10.32YY11 pKa = 7.63NAQPVVIDD19 pKa = 4.41GIRR22 pKa = 11.84FASKK26 pKa = 11.16AEE28 pKa = 3.68GAYY31 pKa = 11.03YY32 pKa = 9.67MLIRR36 pKa = 11.84NRR38 pKa = 11.84PQKK41 pKa = 9.68ITMQEE46 pKa = 3.73PFEE49 pKa = 4.23ILSAFKK55 pKa = 10.77INGKK59 pKa = 9.85RR60 pKa = 11.84YY61 pKa = 8.93SARR64 pKa = 11.84KK65 pKa = 8.9YY66 pKa = 10.62KK67 pKa = 10.31PDD69 pKa = 2.85FCFYY73 pKa = 10.92DD74 pKa = 3.76GEE76 pKa = 4.31KK77 pKa = 9.92LAKK80 pKa = 10.23VVDD83 pKa = 4.24VKK85 pKa = 11.28GGNATLTTDD94 pKa = 2.67ARR96 pKa = 11.84LRR98 pKa = 11.84MLLFMIRR105 pKa = 11.84YY106 pKa = 7.51KK107 pKa = 10.61IPVTIARR114 pKa = 11.84YY115 pKa = 9.52DD116 pKa = 3.47YY117 pKa = 9.38HH118 pKa = 6.72TGLFTEE124 pKa = 4.6EE125 pKa = 3.98QLL127 pKa = 4.41

Molecular weight: 14.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12218

48

1620

226.3

25.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.651 ± 0.654

0.417 ± 0.112

7.104 ± 0.436

5.672 ± 0.443

3.519 ± 0.211

6.826 ± 0.413

1.727 ± 0.184

5.852 ± 0.201

7.489 ± 0.41

7.906 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.177

5.197 ± 0.225

3.421 ± 0.257

4.289 ± 0.215

4.698 ± 0.386

6.556 ± 0.338

6.638 ± 0.42

6.016 ± 0.241

1.449 ± 0.136

3.929 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski