Amino acid dipepetide frequency for Lactobacillus phage Lrm1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.086AlaAla: 9.086 ± 2.221
0.327AlaCys: 0.327 ± 0.182
6.548AlaAsp: 6.548 ± 1.004
5.975AlaGlu: 5.975 ± 0.72
3.274AlaPhe: 3.274 ± 0.485
5.157AlaGly: 5.157 ± 0.701
1.473AlaHis: 1.473 ± 0.331
4.584AlaIle: 4.584 ± 0.657
7.039AlaLys: 7.039 ± 0.983
7.121AlaLeu: 7.121 ± 0.766
3.192AlaMet: 3.192 ± 0.558
5.566AlaAsn: 5.566 ± 0.629
1.964AlaPro: 1.964 ± 0.364
3.029AlaGln: 3.029 ± 0.644
3.274AlaArg: 3.274 ± 0.505
4.911AlaSer: 4.911 ± 1.067
4.911AlaThr: 4.911 ± 0.611
5.402AlaVal: 5.402 ± 0.738
1.064AlaTrp: 1.064 ± 0.277
2.619AlaTyr: 2.619 ± 0.558
0.0AlaXaa: 0.0 ± 0.0
Cys
0.409CysAla: 0.409 ± 0.236
0.164CysCys: 0.164 ± 0.124
0.164CysAsp: 0.164 ± 0.127
0.327CysGlu: 0.327 ± 0.167
0.246CysPhe: 0.246 ± 0.151
0.327CysGly: 0.327 ± 0.187
0.164CysHis: 0.164 ± 0.107
0.0CysIle: 0.0 ± 0.0
0.082CysLys: 0.082 ± 0.086
0.164CysLeu: 0.164 ± 0.112
0.164CysMet: 0.164 ± 0.114
0.164CysAsn: 0.164 ± 0.125
0.164CysPro: 0.164 ± 0.117
0.409CysGln: 0.409 ± 0.188
0.246CysArg: 0.246 ± 0.152
0.164CysSer: 0.164 ± 0.126
0.491CysThr: 0.491 ± 0.226
0.164CysVal: 0.164 ± 0.132
0.0CysTrp: 0.0 ± 0.0
0.164CysTyr: 0.164 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
4.338AspAla: 4.338 ± 0.464
0.164AspCys: 0.164 ± 0.11
5.812AspAsp: 5.812 ± 1.188
3.683AspGlu: 3.683 ± 0.516
2.947AspPhe: 2.947 ± 0.446
6.794AspGly: 6.794 ± 1.304
1.801AspHis: 1.801 ± 0.439
3.929AspIle: 3.929 ± 0.587
4.42AspLys: 4.42 ± 0.641
5.484AspLeu: 5.484 ± 0.779
2.046AspMet: 2.046 ± 0.381
3.683AspAsn: 3.683 ± 0.702
3.52AspPro: 3.52 ± 0.648
2.374AspGln: 2.374 ± 0.36
2.537AspArg: 2.537 ± 0.49
4.093AspSer: 4.093 ± 0.615
4.256AspThr: 4.256 ± 0.753
4.42AspVal: 4.42 ± 0.623
1.146AspTrp: 1.146 ± 0.265
3.602AspTyr: 3.602 ± 0.496
0.0AspXaa: 0.0 ± 0.0
Glu
5.484GluAla: 5.484 ± 0.799
0.327GluCys: 0.327 ± 0.16
3.356GluAsp: 3.356 ± 0.56
3.029GluGlu: 3.029 ± 0.584
2.292GluPhe: 2.292 ± 0.394
3.602GluGly: 3.602 ± 0.472
0.982GluHis: 0.982 ± 0.261
3.11GluIle: 3.11 ± 0.49
4.829GluLys: 4.829 ± 0.738
5.812GluLeu: 5.812 ± 0.71
1.473GluMet: 1.473 ± 0.374
2.701GluAsn: 2.701 ± 0.454
0.982GluPro: 0.982 ± 0.296
3.11GluGln: 3.11 ± 0.518
2.865GluArg: 2.865 ± 0.602
2.537GluSer: 2.537 ± 0.578
3.602GluThr: 3.602 ± 0.638
3.602GluVal: 3.602 ± 0.491
0.9GluTrp: 0.9 ± 0.285
2.128GluTyr: 2.128 ± 0.503
0.0GluXaa: 0.0 ± 0.0
Phe
3.192PheAla: 3.192 ± 0.399
0.246PheCys: 0.246 ± 0.201
2.456PheAsp: 2.456 ± 0.456
2.128PheGlu: 2.128 ± 0.573
0.819PhePhe: 0.819 ± 0.269
3.029PheGly: 3.029 ± 0.51
0.573PheHis: 0.573 ± 0.194
2.21PheIle: 2.21 ± 0.614
1.637PheLys: 1.637 ± 0.278
1.964PheLeu: 1.964 ± 0.524
1.31PheMet: 1.31 ± 0.275
1.883PheAsn: 1.883 ± 0.482
1.637PhePro: 1.637 ± 0.36
1.719PheGln: 1.719 ± 0.389
1.31PheArg: 1.31 ± 0.375
2.619PheSer: 2.619 ± 0.481
3.192PheThr: 3.192 ± 0.569
1.473PheVal: 1.473 ± 0.294
0.737PheTrp: 0.737 ± 0.225
1.064PheTyr: 1.064 ± 0.338
0.0PheXaa: 0.0 ± 0.0
Gly
4.747GlyAla: 4.747 ± 1.172
0.246GlyCys: 0.246 ± 0.154
5.812GlyAsp: 5.812 ± 0.805
3.52GlyGlu: 3.52 ± 0.553
2.865GlyPhe: 2.865 ± 0.417
3.52GlyGly: 3.52 ± 0.568
1.31GlyHis: 1.31 ± 0.507
4.584GlyIle: 4.584 ± 0.596
7.039GlyLys: 7.039 ± 0.671
3.683GlyLeu: 3.683 ± 0.683
2.046GlyMet: 2.046 ± 0.483
3.52GlyAsn: 3.52 ± 0.499
1.964GlyPro: 1.964 ± 0.723
2.046GlyGln: 2.046 ± 0.327
3.11GlyArg: 3.11 ± 0.546
4.011GlySer: 4.011 ± 0.574
5.32GlyThr: 5.32 ± 1.044
4.093GlyVal: 4.093 ± 0.445
1.228GlyTrp: 1.228 ± 0.324
3.602GlyTyr: 3.602 ± 0.742
0.0GlyXaa: 0.0 ± 0.0
His
1.801HisAla: 1.801 ± 0.353
0.082HisCys: 0.082 ± 0.083
0.9HisAsp: 0.9 ± 0.205
0.409HisGlu: 0.409 ± 0.187
0.573HisPhe: 0.573 ± 0.236
1.146HisGly: 1.146 ± 0.317
0.164HisHis: 0.164 ± 0.117
1.064HisIle: 1.064 ± 0.35
1.146HisLys: 1.146 ± 0.379
1.228HisLeu: 1.228 ± 0.306
0.655HisMet: 0.655 ± 0.25
1.064HisAsn: 1.064 ± 0.337
0.655HisPro: 0.655 ± 0.187
0.573HisGln: 0.573 ± 0.187
1.146HisArg: 1.146 ± 0.438
1.064HisSer: 1.064 ± 0.242
1.555HisThr: 1.555 ± 0.347
1.228HisVal: 1.228 ± 0.336
0.246HisTrp: 0.246 ± 0.191
0.573HisTyr: 0.573 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
5.402IleAla: 5.402 ± 0.722
0.164IleCys: 0.164 ± 0.124
4.584IleAsp: 4.584 ± 0.593
4.093IleGlu: 4.093 ± 0.526
1.392IlePhe: 1.392 ± 0.39
4.093IleGly: 4.093 ± 0.63
0.737IleHis: 0.737 ± 0.207
3.11IleIle: 3.11 ± 0.591
5.075IleLys: 5.075 ± 0.638
3.602IleLeu: 3.602 ± 0.511
1.392IleMet: 1.392 ± 0.307
2.947IleAsn: 2.947 ± 0.491
2.128IlePro: 2.128 ± 0.456
2.292IleGln: 2.292 ± 0.473
2.701IleArg: 2.701 ± 0.611
4.747IleSer: 4.747 ± 0.617
4.584IleThr: 4.584 ± 0.579
3.192IleVal: 3.192 ± 0.435
0.409IleTrp: 0.409 ± 0.146
1.883IleTyr: 1.883 ± 0.489
0.0IleXaa: 0.0 ± 0.0
Lys
6.63LysAla: 6.63 ± 0.789
0.327LysCys: 0.327 ± 0.219
4.338LysAsp: 4.338 ± 0.66
4.584LysGlu: 4.584 ± 0.702
2.456LysPhe: 2.456 ± 0.425
4.993LysGly: 4.993 ± 0.759
1.31LysHis: 1.31 ± 0.262
3.929LysIle: 3.929 ± 0.605
5.239LysLys: 5.239 ± 0.791
6.548LysLeu: 6.548 ± 0.689
1.883LysMet: 1.883 ± 0.4
3.847LysAsn: 3.847 ± 0.627
2.374LysPro: 2.374 ± 0.345
4.093LysGln: 4.093 ± 0.624
5.566LysArg: 5.566 ± 0.754
4.829LysSer: 4.829 ± 0.567
4.666LysThr: 4.666 ± 0.584
3.602LysVal: 3.602 ± 0.475
1.064LysTrp: 1.064 ± 0.284
2.619LysTyr: 2.619 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
7.53LeuAla: 7.53 ± 0.705
0.246LeuCys: 0.246 ± 0.166
5.975LeuAsp: 5.975 ± 0.871
3.847LeuGlu: 3.847 ± 0.44
2.947LeuPhe: 2.947 ± 0.484
4.829LeuGly: 4.829 ± 0.746
0.819LeuHis: 0.819 ± 0.246
5.566LeuIle: 5.566 ± 0.482
6.958LeuLys: 6.958 ± 0.851
6.139LeuLeu: 6.139 ± 0.64
1.964LeuMet: 1.964 ± 0.442
3.029LeuAsn: 3.029 ± 0.571
3.029LeuPro: 3.029 ± 0.33
3.683LeuGln: 3.683 ± 0.517
3.683LeuArg: 3.683 ± 0.575
5.32LeuSer: 5.32 ± 0.692
4.993LeuThr: 4.993 ± 0.596
4.911LeuVal: 4.911 ± 0.698
1.064LeuTrp: 1.064 ± 0.338
2.537LeuTyr: 2.537 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
2.292MetAla: 2.292 ± 0.428
0.082MetCys: 0.082 ± 0.09
1.228MetAsp: 1.228 ± 0.413
0.737MetGlu: 0.737 ± 0.255
0.819MetPhe: 0.819 ± 0.221
2.292MetGly: 2.292 ± 0.389
0.409MetHis: 0.409 ± 0.163
1.228MetIle: 1.228 ± 0.338
1.719MetLys: 1.719 ± 0.381
2.128MetLeu: 2.128 ± 0.42
0.491MetMet: 0.491 ± 0.175
2.21MetAsn: 2.21 ± 0.354
0.982MetPro: 0.982 ± 0.217
1.228MetGln: 1.228 ± 0.279
1.228MetArg: 1.228 ± 0.279
2.374MetSer: 2.374 ± 0.389
2.21MetThr: 2.21 ± 0.524
1.555MetVal: 1.555 ± 0.404
0.082MetTrp: 0.082 ± 0.086
1.146MetTyr: 1.146 ± 0.416
0.0MetXaa: 0.0 ± 0.0
Asn
4.502AsnAla: 4.502 ± 0.769
0.082AsnCys: 0.082 ± 0.086
3.602AsnAsp: 3.602 ± 0.55
3.029AsnGlu: 3.029 ± 0.485
1.473AsnPhe: 1.473 ± 0.359
4.584AsnGly: 4.584 ± 0.471
1.064AsnHis: 1.064 ± 0.24
2.619AsnIle: 2.619 ± 0.359
3.11AsnLys: 3.11 ± 0.503
3.52AsnLeu: 3.52 ± 0.602
1.228AsnMet: 1.228 ± 0.292
3.029AsnAsn: 3.029 ± 0.505
2.292AsnPro: 2.292 ± 0.547
2.537AsnGln: 2.537 ± 0.447
3.52AsnArg: 3.52 ± 0.456
2.865AsnSer: 2.865 ± 0.72
2.537AsnThr: 2.537 ± 0.416
3.274AsnVal: 3.274 ± 0.521
0.655AsnTrp: 0.655 ± 0.245
2.374AsnTyr: 2.374 ± 0.405
0.0AsnXaa: 0.0 ± 0.0
Pro
2.537ProAla: 2.537 ± 0.406
0.082ProCys: 0.082 ± 0.088
3.029ProAsp: 3.029 ± 0.663
2.619ProGlu: 2.619 ± 0.507
1.064ProPhe: 1.064 ± 0.275
1.801ProGly: 1.801 ± 0.468
0.327ProHis: 0.327 ± 0.156
2.128ProIle: 2.128 ± 0.469
3.11ProLys: 3.11 ± 0.561
2.783ProLeu: 2.783 ± 0.475
0.573ProMet: 0.573 ± 0.275
2.374ProAsn: 2.374 ± 0.547
0.655ProPro: 0.655 ± 0.319
1.719ProGln: 1.719 ± 0.557
0.9ProArg: 0.9 ± 0.263
2.947ProSer: 2.947 ± 0.377
1.801ProThr: 1.801 ± 0.397
2.456ProVal: 2.456 ± 0.609
0.246ProTrp: 0.246 ± 0.131
1.064ProTyr: 1.064 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
4.584GlnAla: 4.584 ± 0.788
0.082GlnCys: 0.082 ± 0.086
1.555GlnAsp: 1.555 ± 0.309
2.865GlnGlu: 2.865 ± 0.467
1.31GlnPhe: 1.31 ± 0.256
2.537GlnGly: 2.537 ± 0.597
0.982GlnHis: 0.982 ± 0.244
2.865GlnIle: 2.865 ± 0.51
3.356GlnLys: 3.356 ± 0.542
4.502GlnLeu: 4.502 ± 0.592
1.31GlnMet: 1.31 ± 0.407
1.473GlnAsn: 1.473 ± 0.331
1.064GlnPro: 1.064 ± 0.315
2.374GlnGln: 2.374 ± 0.49
1.555GlnArg: 1.555 ± 0.314
2.456GlnSer: 2.456 ± 0.401
2.619GlnThr: 2.619 ± 0.408
2.701GlnVal: 2.701 ± 0.485
0.573GlnTrp: 0.573 ± 0.21
2.128GlnTyr: 2.128 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
3.52ArgAla: 3.52 ± 0.521
0.246ArgCys: 0.246 ± 0.145
3.683ArgAsp: 3.683 ± 0.69
3.274ArgGlu: 3.274 ± 0.611
2.21ArgPhe: 2.21 ± 0.354
2.456ArgGly: 2.456 ± 0.432
1.228ArgHis: 1.228 ± 0.334
3.192ArgIle: 3.192 ± 0.416
3.52ArgLys: 3.52 ± 0.637
3.765ArgLeu: 3.765 ± 0.597
1.228ArgMet: 1.228 ± 0.285
1.964ArgAsn: 1.964 ± 0.398
1.228ArgPro: 1.228 ± 0.313
2.046ArgGln: 2.046 ± 0.437
1.473ArgArg: 1.473 ± 0.338
2.537ArgSer: 2.537 ± 0.392
2.783ArgThr: 2.783 ± 0.47
3.356ArgVal: 3.356 ± 0.584
0.819ArgTrp: 0.819 ± 0.195
2.456ArgTyr: 2.456 ± 0.498
0.0ArgXaa: 0.0 ± 0.0
Ser
4.502SerAla: 4.502 ± 0.752
0.164SerCys: 0.164 ± 0.133
3.929SerAsp: 3.929 ± 0.85
3.438SerGlu: 3.438 ± 0.565
2.537SerPhe: 2.537 ± 0.437
5.566SerGly: 5.566 ± 0.864
0.737SerHis: 0.737 ± 0.244
3.929SerIle: 3.929 ± 0.582
5.484SerLys: 5.484 ± 0.95
5.239SerLeu: 5.239 ± 0.66
2.128SerMet: 2.128 ± 0.376
3.602SerAsn: 3.602 ± 0.543
1.719SerPro: 1.719 ± 0.462
2.537SerGln: 2.537 ± 0.433
3.356SerArg: 3.356 ± 0.427
4.584SerSer: 4.584 ± 0.912
4.666SerThr: 4.666 ± 0.554
4.011SerVal: 4.011 ± 0.477
0.819SerTrp: 0.819 ± 0.297
2.619SerTyr: 2.619 ± 0.45
0.0SerXaa: 0.0 ± 0.0
Thr
6.221ThrAla: 6.221 ± 1.503
0.327ThrCys: 0.327 ± 0.157
5.648ThrAsp: 5.648 ± 0.61
2.865ThrGlu: 2.865 ± 0.507
2.128ThrPhe: 2.128 ± 0.372
4.993ThrGly: 4.993 ± 0.507
1.228ThrHis: 1.228 ± 0.392
3.929ThrIle: 3.929 ± 0.456
3.683ThrLys: 3.683 ± 0.491
6.303ThrLeu: 6.303 ± 0.575
1.146ThrMet: 1.146 ± 0.29
2.701ThrAsn: 2.701 ± 0.482
3.356ThrPro: 3.356 ± 0.533
2.128ThrGln: 2.128 ± 0.363
2.046ThrArg: 2.046 ± 0.425
4.584ThrSer: 4.584 ± 0.665
4.829ThrThr: 4.829 ± 0.696
3.683ThrVal: 3.683 ± 0.541
1.146ThrTrp: 1.146 ± 0.27
3.438ThrTyr: 3.438 ± 0.742
0.0ThrXaa: 0.0 ± 0.0
Val
4.747ValAla: 4.747 ± 0.624
0.246ValCys: 0.246 ± 0.136
4.584ValAsp: 4.584 ± 0.829
3.029ValGlu: 3.029 ± 0.734
1.473ValPhe: 1.473 ± 0.388
3.356ValGly: 3.356 ± 0.539
1.146ValHis: 1.146 ± 0.216
3.52ValIle: 3.52 ± 0.548
4.011ValLys: 4.011 ± 0.625
5.402ValLeu: 5.402 ± 0.501
1.719ValMet: 1.719 ± 0.311
3.602ValAsn: 3.602 ± 0.573
2.374ValPro: 2.374 ± 0.467
1.801ValGln: 1.801 ± 0.322
3.192ValArg: 3.192 ± 0.527
5.075ValSer: 5.075 ± 0.565
3.683ValThr: 3.683 ± 0.556
4.502ValVal: 4.502 ± 0.63
0.982ValTrp: 0.982 ± 0.269
1.719ValTyr: 1.719 ± 0.359
0.0ValXaa: 0.0 ± 0.0
Trp
1.31TrpAla: 1.31 ± 0.406
0.327TrpCys: 0.327 ± 0.21
0.9TrpAsp: 0.9 ± 0.304
0.737TrpGlu: 0.737 ± 0.24
0.327TrpPhe: 0.327 ± 0.166
0.327TrpGly: 0.327 ± 0.16
0.327TrpHis: 0.327 ± 0.195
1.064TrpIle: 1.064 ± 0.289
0.982TrpLys: 0.982 ± 0.274
0.982TrpLeu: 0.982 ± 0.281
0.0TrpMet: 0.0 ± 0.0
0.9TrpAsn: 0.9 ± 0.527
0.164TrpPro: 0.164 ± 0.103
0.982TrpGln: 0.982 ± 0.241
1.228TrpArg: 1.228 ± 0.331
1.064TrpSer: 1.064 ± 0.297
1.146TrpThr: 1.146 ± 0.26
0.982TrpVal: 0.982 ± 0.285
0.491TrpTrp: 0.491 ± 0.24
0.246TrpTyr: 0.246 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.683TyrAla: 3.683 ± 0.606
0.246TyrCys: 0.246 ± 0.144
2.947TyrAsp: 2.947 ± 0.566
2.537TyrGlu: 2.537 ± 0.4
2.046TyrPhe: 2.046 ± 0.383
2.865TyrGly: 2.865 ± 0.677
0.491TyrHis: 0.491 ± 0.209
1.964TyrIle: 1.964 ± 0.63
2.456TyrLys: 2.456 ± 0.477
2.701TyrLeu: 2.701 ± 0.595
0.409TyrMet: 0.409 ± 0.152
1.719TyrAsn: 1.719 ± 0.419
1.964TyrPro: 1.964 ± 0.411
2.21TyrGln: 2.21 ± 0.306
2.046TyrArg: 2.046 ± 0.447
2.783TyrSer: 2.783 ± 0.484
2.537TyrThr: 2.537 ± 0.548
1.555TyrVal: 1.555 ± 0.34
0.819TyrTrp: 0.819 ± 0.266
1.31TyrTyr: 1.31 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12218 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski