Aedes alboannulatus toti-like virus 1
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTD3|A0A1Z2RTD3_9VIRU RNA-directed RNA polymerase OS=Aedes alboannulatus toti-like virus 1 OX=2010265 PE=4 SV=1
MM1 pKa = 8.07 DD2 pKa = 5.02 FNSEE6 pKa = 4.33 SVKK9 pKa = 10.75 KK10 pKa = 10.27 EE11 pKa = 3.77 EE12 pKa = 4.24 EE13 pKa = 4.06 KK14 pKa = 10.46 TGVSCEE20 pKa = 4.25 GQPHH24 pKa = 6.71 PDD26 pKa = 3.09 KK27 pKa = 11.47 VEE29 pKa = 3.8 VMTLTSSIDD38 pKa = 3.75 LVSLEE43 pKa = 4.56 PVCPQATEE51 pKa = 4.08 LKK53 pKa = 10.1 EE54 pKa = 3.97 KK55 pKa = 10.37 EE56 pKa = 4.18 KK57 pKa = 10.94 KK58 pKa = 10.07 EE59 pKa = 4.04 KK60 pKa = 10.56 EE61 pKa = 4.15 KK62 pKa = 9.75 EE63 pKa = 3.99 TTMVVVQSPPVIEE76 pKa = 4.46 TRR78 pKa = 11.84 SSTTEE83 pKa = 3.9 PVPSGPPAPHH93 pKa = 6.35 YY94 pKa = 10.04 GEE96 pKa = 5.1 HH97 pKa = 6.77 SIIPQDD103 pKa = 3.77 VQPVEE108 pKa = 4.2 PAVGLPVGQDD118 pKa = 3.52 VIRR121 pKa = 11.84 KK122 pKa = 7.04 MSSSLMTVARR132 pKa = 11.84 RR133 pKa = 11.84 LVWGGNDD140 pKa = 3.12 YY141 pKa = 11.2 AVRR144 pKa = 11.84 VKK146 pKa = 10.76 DD147 pKa = 3.57 YY148 pKa = 8.82 LTWPRR153 pKa = 11.84 IIGPPLGASVEE164 pKa = 4.28 STITIMHH171 pKa = 6.93 GEE173 pKa = 4.03 HH174 pKa = 7.63 DD175 pKa = 4.54 NIQRR179 pKa = 11.84 VAIKK183 pKa = 8.7 EE184 pKa = 3.83 TAPRR188 pKa = 11.84 IKK190 pKa = 10.73 AEE192 pKa = 3.87 WSVQDD197 pKa = 4.28 EE198 pKa = 4.23 PSILIGSTPDD208 pKa = 3.08 VNKK211 pKa = 10.19 IANKK215 pKa = 10.06 LRR217 pKa = 11.84 GRR219 pKa = 11.84 CDD221 pKa = 2.81 ISKK224 pKa = 10.94 ALAEE228 pKa = 4.27 KK229 pKa = 10.01 LACRR233 pKa = 11.84 INKK236 pKa = 8.9 NLQIHH241 pKa = 6.53 DD242 pKa = 3.9 LSGFFTSGFVLAAHH256 pKa = 5.83 VQLFGCYY263 pKa = 9.9 DD264 pKa = 3.34 IAQNVVPLAADD275 pKa = 3.84 LVITANISVHH285 pKa = 6.8 AEE287 pKa = 3.79 AEE289 pKa = 4.43 VAQTLTSCLKK299 pKa = 10.65 ALNEE303 pKa = 3.96 QALSFDD309 pKa = 3.68 ASTLSEE315 pKa = 3.87 TDD317 pKa = 2.84 IQVIMAIAAGGPSGVITMEE336 pKa = 4.01 GARR339 pKa = 11.84 LYY341 pKa = 10.95 AATSFLDD348 pKa = 3.25 WPTRR352 pKa = 11.84 CYY354 pKa = 10.4 FIYY357 pKa = 10.07 SAKK360 pKa = 10.23 PFALPARR367 pKa = 11.84 RR368 pKa = 11.84 ALTCGEE374 pKa = 4.04 IRR376 pKa = 11.84 ASLYY380 pKa = 11.15 GIATRR385 pKa = 11.84 LNRR388 pKa = 11.84 FDD390 pKa = 5.38 DD391 pKa = 3.68 MAAGFVRR398 pKa = 11.84 SSTFLVGYY406 pKa = 8.63 VRR408 pKa = 11.84 SWSRR412 pKa = 11.84 AAPLAEE418 pKa = 4.31 GASPARR424 pKa = 11.84 SDD426 pKa = 4.43 ARR428 pKa = 11.84 RR429 pKa = 11.84 QHH431 pKa = 5.44 YY432 pKa = 9.86 RR433 pKa = 11.84 GHH435 pKa = 6.8 FYY437 pKa = 11.13 ALHH440 pKa = 5.26 EE441 pKa = 4.66 TNVILVPKK449 pKa = 9.43 VRR451 pKa = 11.84 GYY453 pKa = 11.39 NFLWDD458 pKa = 4.44 LMGTSQPRR466 pKa = 11.84 TAVDD470 pKa = 3.82 DD471 pKa = 4.36 LLVPDD476 pKa = 5.28 GKK478 pKa = 10.69 ALCGLNDD485 pKa = 3.9 EE486 pKa = 4.62 EE487 pKa = 5.58 VMRR490 pKa = 11.84 TTLGIGALLSCSASSVLNYY509 pKa = 10.26 FNLTARR515 pKa = 11.84 EE516 pKa = 4.18 LNTWAAGVGVASTTHH531 pKa = 6.15 LKK533 pKa = 11.19 AMFEE537 pKa = 4.41 SSTPGLPAPYY547 pKa = 9.66 YY548 pKa = 10.3 LATIGFAQQFAAVNIHH564 pKa = 5.65 PHH566 pKa = 6.3 CFSGMYY572 pKa = 10.03 NFSGLGAGMLHH583 pKa = 6.15 VRR585 pKa = 11.84 DD586 pKa = 4.88 DD587 pKa = 3.89 ATWWWGHH594 pKa = 5.69 FGIAVPYY601 pKa = 9.79 LVQPLSMAWIWKK613 pKa = 9.42 SLADD617 pKa = 3.03 IWGCYY622 pKa = 8.51 GPNPVVDD629 pKa = 4.57 FGRR632 pKa = 11.84 DD633 pKa = 3.14 IVIGGAVDD641 pKa = 3.51 NEE643 pKa = 4.21 YY644 pKa = 10.05 MCFNRR649 pKa = 11.84 DD650 pKa = 2.91 EE651 pKa = 4.29 PLYY654 pKa = 10.81 LRR656 pKa = 11.84 LASAPAPYY664 pKa = 10.46 VYY666 pKa = 9.93 IPYY669 pKa = 10.06 GQFAINIIRR678 pKa = 11.84 QYY680 pKa = 11.61 GRR682 pKa = 11.84 ITNVRR687 pKa = 11.84 WVIDD691 pKa = 3.41 MRR693 pKa = 11.84 EE694 pKa = 4.1 VARR697 pKa = 11.84 VAQGAGDD704 pKa = 4.73 LGPPIINQHH713 pKa = 5.44 QPTFAPGLGFIQTGTLLTFDD733 pKa = 4.64 WDD735 pKa = 4.02 LQCILVPGLSKK746 pKa = 10.73 EE747 pKa = 4.52 VVGEE751 pKa = 3.88 ALFMDD756 pKa = 4.74 VCRR759 pKa = 11.84 MGKK762 pKa = 9.54 RR763 pKa = 11.84 ALSDD767 pKa = 3.27 VGVLRR772 pKa = 11.84 DD773 pKa = 4.13 RR774 pKa = 11.84 MDD776 pKa = 3.68 TAPFAPIDD784 pKa = 3.71 TSTMVGLIKK793 pKa = 10.65 GMSAGMSLTEE803 pKa = 4.47 KK804 pKa = 10.35 VDD806 pKa = 3.31 KK807 pKa = 11.18 AGNN810 pKa = 3.42
Molecular weight: 88.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.624
IPC2_protein 5.614
IPC_protein 5.652
Toseland 5.982
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.919
Wikipedia 5.881
Rodwell 5.868
Grimsley 6.097
Solomon 5.893
Lehninger 5.881
Nozaki 6.148
DTASelect 6.326
Thurlkill 6.313
EMBOSS 6.275
Sillero 6.237
Patrickios 4.037
IPC_peptide 5.906
IPC2_peptide 6.237
IPC2.peptide.svr19 6.201
Protein with the highest isoelectric point:
>tr|A0A1Z2RTD3|A0A1Z2RTD3_9VIRU RNA-directed RNA polymerase OS=Aedes alboannulatus toti-like virus 1 OX=2010265 PE=4 SV=1
MM1 pKa = 7.21 SHH3 pKa = 6.85 PHH5 pKa = 5.26 VCLLSTLRR13 pKa = 11.84 IVEE16 pKa = 4.27 EE17 pKa = 4.66 WYY19 pKa = 10.96 HH20 pKa = 5.45 MLWLHH25 pKa = 6.59 PATYY29 pKa = 10.11 NKK31 pKa = 8.41 WMQMNGGDD39 pKa = 3.93 YY40 pKa = 10.66 QLGNSPSCMSLWDD53 pKa = 4.05 EE54 pKa = 4.11 VDD56 pKa = 3.66 SEE58 pKa = 4.58 YY59 pKa = 11.63 KK60 pKa = 10.84 LIVKK64 pKa = 7.8 TRR66 pKa = 11.84 NSTSSIDD73 pKa = 3.38 FAAYY77 pKa = 9.81 RR78 pKa = 11.84 EE79 pKa = 4.4 LVSGSSCIEE88 pKa = 3.73 LVKK91 pKa = 10.61 CRR93 pKa = 11.84 HH94 pKa = 6.21 CNNGASVNIEE104 pKa = 3.62 WSNIKK109 pKa = 10.4 AKK111 pKa = 10.7 SDD113 pKa = 4.0 LNCLDD118 pKa = 4.9 LIWRR122 pKa = 11.84 YY123 pKa = 10.6 SSDD126 pKa = 3.34 ADD128 pKa = 3.5 EE129 pKa = 4.81 SKK131 pKa = 10.24 TNISASQIIWHH142 pKa = 5.53 CRR144 pKa = 11.84 KK145 pKa = 9.85 RR146 pKa = 11.84 KK147 pKa = 8.86 EE148 pKa = 3.98 FQRR151 pKa = 11.84 MFGMFIPMLGVTASNVTATAIAIHH175 pKa = 5.94 SLGNPSAEE183 pKa = 3.91 WIARR187 pKa = 11.84 LLIKK191 pKa = 10.42 KK192 pKa = 8.89 RR193 pKa = 11.84 ICCLDD198 pKa = 3.18 PKK200 pKa = 10.73 GYY202 pKa = 10.71 SKK204 pKa = 10.89 VFKK207 pKa = 10.19 SISIAIRR214 pKa = 11.84 RR215 pKa = 11.84 SSHH218 pKa = 5.29 WPDD221 pKa = 2.8 GRR223 pKa = 11.84 KK224 pKa = 8.22 ATLEE228 pKa = 3.98 EE229 pKa = 4.45 VAQSSGWEE237 pKa = 3.86 LAIGRR242 pKa = 11.84 SSNTSDD248 pKa = 3.05 WIEE251 pKa = 3.9 EE252 pKa = 3.75 RR253 pKa = 11.84 RR254 pKa = 11.84 KK255 pKa = 10.32 RR256 pKa = 11.84 ISCKK260 pKa = 7.78 MHH262 pKa = 6.85 LRR264 pKa = 11.84 MPQNPNFDD272 pKa = 3.7 EE273 pKa = 4.58 EE274 pKa = 4.97 SNEE277 pKa = 4.2 EE278 pKa = 4.08 YY279 pKa = 10.71 CKK281 pKa = 10.61 LLRR284 pKa = 11.84 VQLSALVRR292 pKa = 11.84 LLLIDD297 pKa = 4.34 RR298 pKa = 11.84 EE299 pKa = 4.43 FTTSYY304 pKa = 11.42 EE305 pKa = 4.04 EE306 pKa = 4.09 FVKK309 pKa = 10.58 NRR311 pKa = 11.84 QSWVSSGSTGGHH323 pKa = 6.19 KK324 pKa = 10.06 IIVEE328 pKa = 4.08 GRR330 pKa = 11.84 LEE332 pKa = 3.94 RR333 pKa = 11.84 VNKK336 pKa = 10.04 HH337 pKa = 5.19 VLFEE341 pKa = 4.14 SLRR344 pKa = 11.84 TEE346 pKa = 4.84 EE347 pKa = 3.96 MVAWLDD353 pKa = 3.75 DD354 pKa = 4.01 EE355 pKa = 4.8 PRR357 pKa = 11.84 TEE359 pKa = 4.08 AVASEE364 pKa = 4.18 KK365 pKa = 11.04 FEE367 pKa = 4.06 MGKK370 pKa = 10.11 ARR372 pKa = 11.84 AIYY375 pKa = 7.6 GTKK378 pKa = 10.08 PIDD381 pKa = 3.67 YY382 pKa = 8.2 TISSYY387 pKa = 11.13 VLNDD391 pKa = 3.1 IEE393 pKa = 4.79 PRR395 pKa = 11.84 LSVIPGIEE403 pKa = 3.98 SGLNGMDD410 pKa = 4.35 IIASVANRR418 pKa = 11.84 KK419 pKa = 7.51 ATAMQPRR426 pKa = 11.84 TEE428 pKa = 4.4 CSMIDD433 pKa = 3.54 YY434 pKa = 11.14 ADD436 pKa = 4.05 FNYY439 pKa = 10.22 QHH441 pKa = 6.66 TLQAQALVFDD451 pKa = 4.75 ALADD455 pKa = 3.54 EE456 pKa = 4.97 FEE458 pKa = 5.5 RR459 pKa = 11.84 IGAHH463 pKa = 5.99 PDD465 pKa = 2.77 KK466 pKa = 11.01 VRR468 pKa = 11.84 ASRR471 pKa = 11.84 WTRR474 pKa = 11.84 DD475 pKa = 2.92 ALLNQWCRR483 pKa = 11.84 FPLRR487 pKa = 11.84 SKK489 pKa = 10.17 PDD491 pKa = 3.82 KK492 pKa = 9.39 ITQGMFSGCRR502 pKa = 11.84 GTNFLNTLLNEE513 pKa = 4.36 AYY515 pKa = 10.45 FLVAMNWVKK524 pKa = 10.04 TYY526 pKa = 11.43 LNLSPLYY533 pKa = 10.3 LFNIHH538 pKa = 6.39 QGDD541 pKa = 4.17 DD542 pKa = 3.17 VWISNEE548 pKa = 3.66 SRR550 pKa = 11.84 LWAVGLFQSMQQSGFVFQASKK571 pKa = 11.24 QMFDD575 pKa = 2.93 VCRR578 pKa = 11.84 AEE580 pKa = 4.05 FLRR583 pKa = 11.84 VLYY586 pKa = 10.6 SDD588 pKa = 4.48 EE589 pKa = 4.46 GCVGYY594 pKa = 8.12 MARR597 pKa = 11.84 AIAALIIKK605 pKa = 7.83 PIQSSDD611 pKa = 3.27 VTGPAEE617 pKa = 4.19 RR618 pKa = 11.84 AISLHH623 pKa = 5.03 GQICIISRR631 pKa = 11.84 RR632 pKa = 11.84 GFSPDD637 pKa = 3.0 SVGLLWRR644 pKa = 11.84 AMVPYY649 pKa = 9.66 GARR652 pKa = 11.84 VNLPRR657 pKa = 11.84 GGLTIPMSILYY668 pKa = 7.23 LHH670 pKa = 7.32 PSRR673 pKa = 11.84 GGLGLVPPGEE683 pKa = 4.31 MSDD686 pKa = 3.19 SKK688 pKa = 11.31 AAVKK692 pKa = 10.11 AVPTFEE698 pKa = 4.9 ASSKK702 pKa = 10.47 ALEE705 pKa = 4.19 EE706 pKa = 4.03 AVPCNMSRR714 pKa = 11.84 DD715 pKa = 3.55 WVVLVSEE722 pKa = 4.98 RR723 pKa = 11.84 VQKK726 pKa = 10.89 EE727 pKa = 3.85 FDD729 pKa = 3.69 SKK731 pKa = 11.65 ALLDD735 pKa = 3.88 MVHH738 pKa = 6.92 ASNVTDD744 pKa = 3.52 SLRR747 pKa = 11.84 PIDD750 pKa = 4.33 KK751 pKa = 10.54 LWGLRR756 pKa = 11.84 ALEE759 pKa = 4.39 DD760 pKa = 4.2 NLRR763 pKa = 11.84 KK764 pKa = 9.31 WKK766 pKa = 10.61 AGLVLPRR773 pKa = 11.84 VVCDD777 pKa = 3.92 KK778 pKa = 11.32 SIMDD782 pKa = 4.22 AFFSEE787 pKa = 4.26 AGFYY791 pKa = 10.16 PDD793 pKa = 4.52 LEE795 pKa = 4.21 RR796 pKa = 11.84 AIEE799 pKa = 4.29 DD800 pKa = 3.85 LLSGKK805 pKa = 7.44 MTKK808 pKa = 10.09 KK809 pKa = 8.6 SQRR812 pKa = 11.84 RR813 pKa = 11.84 RR814 pKa = 11.84 GPASYY819 pKa = 10.85 INLAVSMSPFKK830 pKa = 10.69 SLPAAAVSMKK840 pKa = 10.47 GDD842 pKa = 3.03 WEE844 pKa = 4.67 TIVRR848 pKa = 11.84 ACISMCINDD857 pKa = 4.58 YY858 pKa = 10.38 IRR860 pKa = 11.84 EE861 pKa = 4.1 VASYY865 pKa = 10.72 SVEE868 pKa = 4.04 EE869 pKa = 4.03 LLKK872 pKa = 9.49 TVGVGVTRR880 pKa = 11.84 CLVEE884 pKa = 3.97 GQNVGLSNFEE894 pKa = 4.52 VFWHH898 pKa = 6.53 PIILSWVHH906 pKa = 6.16 DD907 pKa = 3.84 MAMEE911 pKa = 4.7 RR912 pKa = 11.84 ATLGLIVAGTQDD924 pKa = 3.27 VPQAKK929 pKa = 10.01 LYY931 pKa = 9.45 VQSEE935 pKa = 4.3 FDD937 pKa = 3.18 NAIRR941 pKa = 11.84 VLNKK945 pKa = 9.98 FKK947 pKa = 11.17 DD948 pKa = 3.44 MRR950 pKa = 11.84 EE951 pKa = 3.84 LSKK954 pKa = 11.21 YY955 pKa = 10.14
Molecular weight: 107.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.951
IPC2_protein 7.337
IPC_protein 7.132
Toseland 6.839
ProMoST 7.819
Dawson 7.849
Bjellqvist 8.302
Wikipedia 7.746
Rodwell 7.863
Grimsley 6.898
Solomon 7.878
Lehninger 7.892
Nozaki 8.551
DTASelect 8.009
Thurlkill 8.053
EMBOSS 8.068
Sillero 8.404
Patrickios 4.38
IPC_peptide 7.863
IPC2_peptide 7.38
IPC2.peptide.svr19 7.317
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1765
810
955
882.5
98.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.669 ± 0.817
2.096 ± 0.165
5.099 ± 0.058
5.722 ± 0.447
3.569 ± 0.007
6.572 ± 0.815
2.04 ± 0.04
6.176 ± 0.085
4.533 ± 0.56
8.952 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.173 ± 0.225
3.739 ± 0.358
4.929 ± 0.841
3.059 ± 0.102
6.006 ± 0.555
8.329 ± 1.04
4.703 ± 0.827
7.479 ± 0.453
2.266 ± 0.28
2.89 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here