Changjiang tombus-like virus 20
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFX6|A0A1L3KFX6_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 20 OX=1922814 PE=4 SV=1
MM1 pKa = 8.14 RR2 pKa = 11.84 YY3 pKa = 9.31 HH4 pKa = 7.3 GNYY7 pKa = 9.68 CGPSWSDD14 pKa = 3.0 GKK16 pKa = 10.27 QQLSVAAGDD25 pKa = 3.7 QPAIDD30 pKa = 5.58 DD31 pKa = 3.89 FDD33 pKa = 4.05 KK34 pKa = 11.01 TCEE37 pKa = 3.94 IHH39 pKa = 7.51 DD40 pKa = 4.17 KK41 pKa = 11.17 NYY43 pKa = 11.12 ALNTNLYY50 pKa = 9.11 EE51 pKa = 4.81 ADD53 pKa = 3.17 NSFYY57 pKa = 10.16 MSNYY61 pKa = 9.78 GMGTKK66 pKa = 10.22 RR67 pKa = 11.84 SLAALAVKK75 pKa = 10.29 ANSVLRR81 pKa = 11.84 EE82 pKa = 4.01 YY83 pKa = 11.11 VPNNLPSQVTGPRR96 pKa = 11.84 SFNMVKK102 pKa = 10.27 NAKK105 pKa = 9.7 QPTKK109 pKa = 10.83 LRR111 pKa = 11.84 GAKK114 pKa = 8.99 VAPTKK119 pKa = 10.29 RR120 pKa = 11.84 TVTKK124 pKa = 10.66 NSQQLSTVPASYY136 pKa = 10.73 GFSLRR141 pKa = 11.84 MQEE144 pKa = 4.13 PRR146 pKa = 11.84 VQRR149 pKa = 11.84 TGKK152 pKa = 7.98 NTVIVGSDD160 pKa = 3.53 YY161 pKa = 10.22 ATSVATSISTGTYY174 pKa = 9.54 APSGSILLNPSYY186 pKa = 10.13 FASAMLGNVARR197 pKa = 11.84 TYY199 pKa = 11.07 EE200 pKa = 4.03 KK201 pKa = 10.76 FRR203 pKa = 11.84 FTKK206 pKa = 10.57 AVIQYY211 pKa = 9.04 IPQVPTTTAGQIVMCSTRR229 pKa = 11.84 TVKK232 pKa = 10.54 EE233 pKa = 3.44 PFINGNSPTFLSRR246 pKa = 11.84 ALSQGNAVASPLWKK260 pKa = 9.12 EE261 pKa = 3.84 TTLTVNCSPEE271 pKa = 3.48 WSIVDD276 pKa = 4.14 GYY278 pKa = 10.79 IDD280 pKa = 4.02 TDD282 pKa = 4.07 LDD284 pKa = 3.81 DD285 pKa = 5.64 SIQEE289 pKa = 3.99 EE290 pKa = 4.45 VQIYY294 pKa = 5.46 TTCDD298 pKa = 2.97 SVLTTGIIVLHH309 pKa = 4.95 YY310 pKa = 9.3 TIEE313 pKa = 4.5 FKK315 pKa = 11.06 DD316 pKa = 3.5 PLYY319 pKa = 10.75 SYY321 pKa = 10.7 HH322 pKa = 7.22 SNNIPNPIGNGVFDD336 pKa = 4.79 AFSDD340 pKa = 3.71 NTPINAVGDD349 pKa = 3.95 ALILSNSSTTTGTPSGTIFRR369 pKa = 11.84 MVFRR373 pKa = 11.84 QQRR376 pKa = 11.84 STLPTGVPQWNALANTVTQASSSLATFTNQITSIAMNPGTVLYY419 pKa = 10.33 GLQATGGVALFATLEE434 pKa = 4.16 SAKK437 pKa = 10.44 AGASGYY443 pKa = 7.97 ITYY446 pKa = 10.87 ASATTVGGVYY456 pKa = 9.53 QFIISIVQVGPSIAITTQQ474 pKa = 2.68
Molecular weight: 51.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.606
IPC2_protein 7.834
IPC_protein 7.761
Toseland 7.483
ProMoST 8.17
Dawson 8.404
Bjellqvist 8.609
Wikipedia 8.39
Rodwell 8.419
Grimsley 7.454
Solomon 8.536
Lehninger 8.521
Nozaki 8.639
DTASelect 8.419
Thurlkill 8.463
EMBOSS 8.595
Sillero 8.726
Patrickios 3.745
IPC_peptide 8.521
IPC2_peptide 7.541
IPC2.peptide.svr19 7.826
Protein with the highest isoelectric point:
>tr|A0A1L3KFX6|A0A1L3KFX6_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 20 OX=1922814 PE=4 SV=1
MM1 pKa = 7.48 PRR3 pKa = 11.84 ASQVKK8 pKa = 8.84 TSRR11 pKa = 11.84 QPGNRR16 pKa = 11.84 ASPVTQKK23 pKa = 8.88 NTNRR27 pKa = 11.84 DD28 pKa = 3.45 SCSSSASTTDD38 pKa = 3.24 TVSDD42 pKa = 4.25 LVTDD46 pKa = 4.31 ARR48 pKa = 11.84 PGNALGEE55 pKa = 4.41 APSNLSKK62 pKa = 10.72 LGKK65 pKa = 10.23 DD66 pKa = 3.12 GGTAGSAAEE75 pKa = 4.11 APLVGEE81 pKa = 4.87 LEE83 pKa = 4.42 HH84 pKa = 7.14 KK85 pKa = 10.5 RR86 pKa = 11.84 GPKK89 pKa = 9.45 SPKK92 pKa = 8.73 EE93 pKa = 3.83 KK94 pKa = 9.64 PGNGVRR100 pKa = 11.84 VKK102 pKa = 10.77 SKK104 pKa = 9.18 TLSPQDD110 pKa = 3.39 IKK112 pKa = 11.53 NKK114 pKa = 10.25 SNSTKK119 pKa = 9.49 EE120 pKa = 4.26 TVLSGARR127 pKa = 11.84 RR128 pKa = 11.84 KK129 pKa = 9.12 AAPGVRR135 pKa = 11.84 LLTQNVMVSRR145 pKa = 11.84 NIKK148 pKa = 8.13 NTHH151 pKa = 5.73 APIAGGQDD159 pKa = 3.07 AMHH162 pKa = 6.26 QGIGHH167 pKa = 6.66 EE168 pKa = 4.32 GRR170 pKa = 11.84 IKK172 pKa = 9.9 FTRR175 pKa = 11.84 PWVISPEE182 pKa = 3.9 LQIILKK188 pKa = 8.75 TRR190 pKa = 11.84 FPNRR194 pKa = 11.84 LVTTEE199 pKa = 3.55 GRR201 pKa = 11.84 KK202 pKa = 9.6 LYY204 pKa = 9.82 NHH206 pKa = 6.9 PHH208 pKa = 6.6 PLSAIEE214 pKa = 3.68 RR215 pKa = 11.84 AIIEE219 pKa = 4.11 EE220 pKa = 4.43 EE221 pKa = 3.83 IYY223 pKa = 9.04 EE224 pKa = 4.23 TLKK227 pKa = 11.38 NNDD230 pKa = 3.42 CNLIKK235 pKa = 10.66 DD236 pKa = 3.47 IGGNASRR243 pKa = 11.84 HH244 pKa = 3.77 KK245 pKa = 8.74 TARR248 pKa = 11.84 RR249 pKa = 11.84 TDD251 pKa = 3.26 VHH253 pKa = 6.43 SCCPILSPKK262 pKa = 10.49 DD263 pKa = 3.29 VLRR266 pKa = 11.84 NNRR269 pKa = 11.84 YY270 pKa = 8.5 NATMNYY276 pKa = 6.87 CTSKK280 pKa = 11.36 AEE282 pKa = 4.26 DD283 pKa = 4.07 CNVVPDD289 pKa = 4.68 GYY291 pKa = 10.23 MAIHH295 pKa = 6.32 SLYY298 pKa = 9.24 YY299 pKa = 10.43 LEE301 pKa = 5.03 PATVLHH307 pKa = 6.69 LVHH310 pKa = 6.65 QSRR313 pKa = 11.84 KK314 pKa = 9.43 KK315 pKa = 9.77 ILIASLHH322 pKa = 6.65 DD323 pKa = 3.93 FSQGYY328 pKa = 10.51 GMMHH332 pKa = 6.17 YY333 pKa = 10.65 NGIEE337 pKa = 3.84 HH338 pKa = 6.15 EE339 pKa = 4.42 TRR341 pKa = 11.84 FQIVDD346 pKa = 3.51 RR347 pKa = 11.84 NTVLMTSSGNDD358 pKa = 3.36 GGPYY362 pKa = 8.19 THH364 pKa = 6.98 SPCFWLRR371 pKa = 11.84 KK372 pKa = 9.18 CYY374 pKa = 10.66 YY375 pKa = 10.06 SDD377 pKa = 3.58 GKK379 pKa = 10.64 RR380 pKa = 11.84 AIAWGYY386 pKa = 9.82 RR387 pKa = 11.84 VVGDD391 pKa = 3.29 TYY393 pKa = 10.93 IYY395 pKa = 10.55 TFTTAPLGLDD405 pKa = 3.46 TVKK408 pKa = 10.52 EE409 pKa = 3.85 RR410 pKa = 11.84 DD411 pKa = 3.41 MDD413 pKa = 4.06 LVSTIRR419 pKa = 11.84 NTSHH423 pKa = 6.87 HH424 pKa = 6.45 GEE426 pKa = 3.94 IGATNYY432 pKa = 10.36 APLMNYY438 pKa = 9.66 LHH440 pKa = 7.46 LDD442 pKa = 3.55 TVVFRR447 pKa = 11.84 SYY449 pKa = 11.62 GPLVWAQKK457 pKa = 10.02 NSRR460 pKa = 11.84 QIFIPKK466 pKa = 10.26 GLIQRR471 pKa = 11.84 VAFNLVGKK479 pKa = 9.17 PRR481 pKa = 11.84 NRR483 pKa = 11.84 DD484 pKa = 3.35 TLIMCVRR491 pKa = 11.84 DD492 pKa = 3.75 TKK494 pKa = 11.15 QEE496 pKa = 3.99 LNKK499 pKa = 10.48 RR500 pKa = 11.84 NLEE503 pKa = 4.16 LPDD506 pKa = 3.54 SVRR509 pKa = 11.84 AEE511 pKa = 3.82 LAVFVPPLAFLLHH524 pKa = 7.12 LEE526 pKa = 5.13 DD527 pKa = 4.49 EE528 pKa = 4.7 VQSFNRR534 pKa = 11.84 LVMPTNQKK542 pKa = 10.37 LYY544 pKa = 10.38 KK545 pKa = 9.63 QLNDD549 pKa = 4.5 AINLVKK555 pKa = 10.38 PYY557 pKa = 10.87 GCLFKK562 pKa = 11.17 CFGKK566 pKa = 10.57 LKK568 pKa = 9.1 HH569 pKa = 5.92 QEE571 pKa = 3.84 SSVVVDD577 pKa = 4.91 EE578 pKa = 4.59 YY579 pKa = 11.63 NSTRR583 pKa = 11.84 TSLDD587 pKa = 3.19 TVKK590 pKa = 10.91 EE591 pKa = 4.13 KK592 pKa = 9.77 ITTGFAFLSTTAKK605 pKa = 10.74 NAMKK609 pKa = 9.95 KK610 pKa = 9.34 QRR612 pKa = 11.84 SGTYY616 pKa = 8.43 FKK618 pKa = 11.12 VAIEE622 pKa = 4.13 EE623 pKa = 4.14 PRR625 pKa = 11.84 EE626 pKa = 3.8 DD627 pKa = 3.87 KK628 pKa = 11.36 YY629 pKa = 11.95 VMKK632 pKa = 10.61 QVATTFSGHH641 pKa = 5.95 IPIVPTKK648 pKa = 8.54 TQSNTEE654 pKa = 3.58 LALRR658 pKa = 11.84 NRR660 pKa = 11.84 VLAEE664 pKa = 3.66 VTPPLEE670 pKa = 4.15 GHH672 pKa = 6.86 WDD674 pKa = 3.32 NMADD678 pKa = 4.01 RR679 pKa = 11.84 FFNKK683 pKa = 9.49 QFLDD687 pKa = 4.13 FTQFDD692 pKa = 4.72 YY693 pKa = 9.9 EE694 pKa = 4.15 TDD696 pKa = 3.33 EE697 pKa = 5.33 DD698 pKa = 4.19 KK699 pKa = 11.66 AFVKK703 pKa = 9.8 WNSGFQPGRR712 pKa = 11.84 QKK714 pKa = 10.79 DD715 pKa = 3.56 HH716 pKa = 5.59 EE717 pKa = 4.16 RR718 pKa = 11.84 ARR720 pKa = 11.84 INLKK724 pKa = 10.64 NEE726 pKa = 4.37 GIKK729 pKa = 10.48 NKK731 pKa = 10.02 HH732 pKa = 5.42 YY733 pKa = 10.12 YY734 pKa = 9.99 RR735 pKa = 11.84 KK736 pKa = 8.15 TFMKK740 pKa = 10.43 VEE742 pKa = 4.14 KK743 pKa = 9.79 LDD745 pKa = 3.53 KK746 pKa = 10.98 SSADD750 pKa = 3.22 GVEE753 pKa = 4.25 DD754 pKa = 4.27 FNPRR758 pKa = 11.84 AISGTSHH765 pKa = 6.78 EE766 pKa = 5.24 SNVVMGPFMTQYY778 pKa = 10.97 SKK780 pKa = 11.15 QLAKK784 pKa = 10.09 QWNGEE789 pKa = 3.77 GTFYY793 pKa = 10.07 YY794 pKa = 10.44 TSGATGEE801 pKa = 4.48 STGKK805 pKa = 9.37 WMYY808 pKa = 11.64 DD809 pKa = 3.01 NFTDD813 pKa = 3.52 GDD815 pKa = 4.63 LIVEE819 pKa = 4.4 VDD821 pKa = 4.48 FSAYY825 pKa = 10.55 DD826 pKa = 3.48 STQSRR831 pKa = 11.84 DD832 pKa = 2.91 SYY834 pKa = 11.15 LFEE837 pKa = 5.28 KK838 pKa = 10.02 RR839 pKa = 11.84 VLMAAGMDD847 pKa = 3.3 KK848 pKa = 10.99 HH849 pKa = 6.28 EE850 pKa = 4.63 AVCEE854 pKa = 4.26 VFKK857 pKa = 10.79 HH858 pKa = 4.63 QSNMRR863 pKa = 11.84 GFTGDD868 pKa = 3.34 GIEE871 pKa = 4.03 YY872 pKa = 9.92 KK873 pKa = 10.66 LPYY876 pKa = 9.31 GRR878 pKa = 11.84 NSGDD882 pKa = 3.62 PNTSCGNSLLTAATTEE898 pKa = 4.16 YY899 pKa = 11.28 VLTKK903 pKa = 10.58 VFGPGDD909 pKa = 3.67 YY910 pKa = 9.98 KK911 pKa = 10.77 IKK913 pKa = 11.05 VMGDD917 pKa = 3.4 DD918 pKa = 3.33 NTSIVKK924 pKa = 8.5 MANANGVEE932 pKa = 3.89 MDD934 pKa = 3.88 EE935 pKa = 4.85 VIEE938 pKa = 3.96 LVKK941 pKa = 10.84 KK942 pKa = 10.45 EE943 pKa = 3.75 FLKK946 pKa = 11.0 FGFVAKK952 pKa = 10.43 VKK954 pKa = 9.79 IHH956 pKa = 6.45 YY957 pKa = 9.52 DD958 pKa = 3.16 VASAEE963 pKa = 4.51 FCSSAYY969 pKa = 9.11 WPATINNHH977 pKa = 4.65 HH978 pKa = 6.93 TYY980 pKa = 11.25 VMGPKK985 pKa = 9.81 PGRR988 pKa = 11.84 LLPKK992 pKa = 8.88 MGYY995 pKa = 9.18 VIKK998 pKa = 10.37 DD999 pKa = 3.56 LSPGEE1004 pKa = 4.06 VKK1006 pKa = 10.72 GMFEE1010 pKa = 5.33 GYY1012 pKa = 10.89 ASTCSHH1018 pKa = 6.35 IPVLKK1023 pKa = 10.51 QYY1025 pKa = 11.09 VNHH1028 pKa = 6.92 CLKK1031 pKa = 10.72 QMEE1034 pKa = 4.63 TIKK1037 pKa = 10.22 TKK1039 pKa = 10.87 NYY1041 pKa = 7.43 TDD1043 pKa = 4.11 KK1044 pKa = 10.5 EE1045 pKa = 4.24 AKK1047 pKa = 10.2 YY1048 pKa = 10.75 KK1049 pKa = 9.44 LTNTGGAVDD1058 pKa = 4.22 NDD1060 pKa = 3.18 QTAAFFLQRR1069 pKa = 11.84 YY1070 pKa = 7.87 GLSLDD1075 pKa = 3.49 MTIDD1079 pKa = 3.4 SLKK1082 pKa = 9.75 RR1083 pKa = 11.84 TLDD1086 pKa = 3.28 GSGPDD1091 pKa = 5.75 DD1092 pKa = 3.58 MVHH1095 pKa = 5.87 WPLLEE1100 pKa = 4.32 VIRR1103 pKa = 11.84 HH1104 pKa = 4.71 VDD1106 pKa = 2.88 AA1107 pKa = 5.92
Molecular weight: 124.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.303
IPC2_protein 8.302
IPC_protein 8.126
Toseland 8.653
ProMoST 8.697
Dawson 9.092
Bjellqvist 9.033
Wikipedia 9.224
Rodwell 9.253
Grimsley 9.033
Solomon 9.121
Lehninger 9.092
Nozaki 9.136
DTASelect 8.902
Thurlkill 9.019
EMBOSS 9.238
Sillero 9.194
Patrickios 4.533
IPC_peptide 9.121
IPC2_peptide 7.688
IPC2.peptide.svr19 7.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1581
474
1107
790.5
87.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.958 ± 1.001
1.328 ± 0.162
5.187 ± 0.822
4.491 ± 1.159
3.732 ± 0.086
7.084 ± 0.053
2.404 ± 0.923
4.87 ± 0.738
6.641 ± 1.683
7.147 ± 0.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.53 ± 0.374
5.946 ± 0.227
4.934 ± 0.326
3.732 ± 0.788
4.681 ± 0.772
7.653 ± 1.339
8.539 ± 1.564
7.021 ± 0.215
0.886 ± 0.025
4.238 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here