Changjiang tombus-like virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFX6|A0A1L3KFX6_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 20 OX=1922814 PE=4 SV=1
MM1 pKa = 8.14RR2 pKa = 11.84YY3 pKa = 9.31HH4 pKa = 7.3GNYY7 pKa = 9.68CGPSWSDD14 pKa = 3.0GKK16 pKa = 10.27QQLSVAAGDD25 pKa = 3.7QPAIDD30 pKa = 5.58DD31 pKa = 3.89FDD33 pKa = 4.05KK34 pKa = 11.01TCEE37 pKa = 3.94IHH39 pKa = 7.51DD40 pKa = 4.17KK41 pKa = 11.17NYY43 pKa = 11.12ALNTNLYY50 pKa = 9.11EE51 pKa = 4.81ADD53 pKa = 3.17NSFYY57 pKa = 10.16MSNYY61 pKa = 9.78GMGTKK66 pKa = 10.22RR67 pKa = 11.84SLAALAVKK75 pKa = 10.29ANSVLRR81 pKa = 11.84EE82 pKa = 4.01YY83 pKa = 11.11VPNNLPSQVTGPRR96 pKa = 11.84SFNMVKK102 pKa = 10.27NAKK105 pKa = 9.7QPTKK109 pKa = 10.83LRR111 pKa = 11.84GAKK114 pKa = 8.99VAPTKK119 pKa = 10.29RR120 pKa = 11.84TVTKK124 pKa = 10.66NSQQLSTVPASYY136 pKa = 10.73GFSLRR141 pKa = 11.84MQEE144 pKa = 4.13PRR146 pKa = 11.84VQRR149 pKa = 11.84TGKK152 pKa = 7.98NTVIVGSDD160 pKa = 3.53YY161 pKa = 10.22ATSVATSISTGTYY174 pKa = 9.54APSGSILLNPSYY186 pKa = 10.13FASAMLGNVARR197 pKa = 11.84TYY199 pKa = 11.07EE200 pKa = 4.03KK201 pKa = 10.76FRR203 pKa = 11.84FTKK206 pKa = 10.57AVIQYY211 pKa = 9.04IPQVPTTTAGQIVMCSTRR229 pKa = 11.84TVKK232 pKa = 10.54EE233 pKa = 3.44PFINGNSPTFLSRR246 pKa = 11.84ALSQGNAVASPLWKK260 pKa = 9.12EE261 pKa = 3.84TTLTVNCSPEE271 pKa = 3.48WSIVDD276 pKa = 4.14GYY278 pKa = 10.79IDD280 pKa = 4.02TDD282 pKa = 4.07LDD284 pKa = 3.81DD285 pKa = 5.64SIQEE289 pKa = 3.99EE290 pKa = 4.45VQIYY294 pKa = 5.46TTCDD298 pKa = 2.97SVLTTGIIVLHH309 pKa = 4.95YY310 pKa = 9.3TIEE313 pKa = 4.5FKK315 pKa = 11.06DD316 pKa = 3.5PLYY319 pKa = 10.75SYY321 pKa = 10.7HH322 pKa = 7.22SNNIPNPIGNGVFDD336 pKa = 4.79AFSDD340 pKa = 3.71NTPINAVGDD349 pKa = 3.95ALILSNSSTTTGTPSGTIFRR369 pKa = 11.84MVFRR373 pKa = 11.84QQRR376 pKa = 11.84STLPTGVPQWNALANTVTQASSSLATFTNQITSIAMNPGTVLYY419 pKa = 10.33GLQATGGVALFATLEE434 pKa = 4.16SAKK437 pKa = 10.44AGASGYY443 pKa = 7.97ITYY446 pKa = 10.87ASATTVGGVYY456 pKa = 9.53QFIISIVQVGPSIAITTQQ474 pKa = 2.68

Molecular weight:
51.0 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFX6|A0A1L3KFX6_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 20 OX=1922814 PE=4 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84ASQVKK8 pKa = 8.84TSRR11 pKa = 11.84QPGNRR16 pKa = 11.84ASPVTQKK23 pKa = 8.88NTNRR27 pKa = 11.84DD28 pKa = 3.45SCSSSASTTDD38 pKa = 3.24TVSDD42 pKa = 4.25LVTDD46 pKa = 4.31ARR48 pKa = 11.84PGNALGEE55 pKa = 4.41APSNLSKK62 pKa = 10.72LGKK65 pKa = 10.23DD66 pKa = 3.12GGTAGSAAEE75 pKa = 4.11APLVGEE81 pKa = 4.87LEE83 pKa = 4.42HH84 pKa = 7.14KK85 pKa = 10.5RR86 pKa = 11.84GPKK89 pKa = 9.45SPKK92 pKa = 8.73EE93 pKa = 3.83KK94 pKa = 9.64PGNGVRR100 pKa = 11.84VKK102 pKa = 10.77SKK104 pKa = 9.18TLSPQDD110 pKa = 3.39IKK112 pKa = 11.53NKK114 pKa = 10.25SNSTKK119 pKa = 9.49EE120 pKa = 4.26TVLSGARR127 pKa = 11.84RR128 pKa = 11.84KK129 pKa = 9.12AAPGVRR135 pKa = 11.84LLTQNVMVSRR145 pKa = 11.84NIKK148 pKa = 8.13NTHH151 pKa = 5.73APIAGGQDD159 pKa = 3.07AMHH162 pKa = 6.26QGIGHH167 pKa = 6.66EE168 pKa = 4.32GRR170 pKa = 11.84IKK172 pKa = 9.9FTRR175 pKa = 11.84PWVISPEE182 pKa = 3.9LQIILKK188 pKa = 8.75TRR190 pKa = 11.84FPNRR194 pKa = 11.84LVTTEE199 pKa = 3.55GRR201 pKa = 11.84KK202 pKa = 9.6LYY204 pKa = 9.82NHH206 pKa = 6.9PHH208 pKa = 6.6PLSAIEE214 pKa = 3.68RR215 pKa = 11.84AIIEE219 pKa = 4.11EE220 pKa = 4.43EE221 pKa = 3.83IYY223 pKa = 9.04EE224 pKa = 4.23TLKK227 pKa = 11.38NNDD230 pKa = 3.42CNLIKK235 pKa = 10.66DD236 pKa = 3.47IGGNASRR243 pKa = 11.84HH244 pKa = 3.77KK245 pKa = 8.74TARR248 pKa = 11.84RR249 pKa = 11.84TDD251 pKa = 3.26VHH253 pKa = 6.43SCCPILSPKK262 pKa = 10.49DD263 pKa = 3.29VLRR266 pKa = 11.84NNRR269 pKa = 11.84YY270 pKa = 8.5NATMNYY276 pKa = 6.87CTSKK280 pKa = 11.36AEE282 pKa = 4.26DD283 pKa = 4.07CNVVPDD289 pKa = 4.68GYY291 pKa = 10.23MAIHH295 pKa = 6.32SLYY298 pKa = 9.24YY299 pKa = 10.43LEE301 pKa = 5.03PATVLHH307 pKa = 6.69LVHH310 pKa = 6.65QSRR313 pKa = 11.84KK314 pKa = 9.43KK315 pKa = 9.77ILIASLHH322 pKa = 6.65DD323 pKa = 3.93FSQGYY328 pKa = 10.51GMMHH332 pKa = 6.17YY333 pKa = 10.65NGIEE337 pKa = 3.84HH338 pKa = 6.15EE339 pKa = 4.42TRR341 pKa = 11.84FQIVDD346 pKa = 3.51RR347 pKa = 11.84NTVLMTSSGNDD358 pKa = 3.36GGPYY362 pKa = 8.19THH364 pKa = 6.98SPCFWLRR371 pKa = 11.84KK372 pKa = 9.18CYY374 pKa = 10.66YY375 pKa = 10.06SDD377 pKa = 3.58GKK379 pKa = 10.64RR380 pKa = 11.84AIAWGYY386 pKa = 9.82RR387 pKa = 11.84VVGDD391 pKa = 3.29TYY393 pKa = 10.93IYY395 pKa = 10.55TFTTAPLGLDD405 pKa = 3.46TVKK408 pKa = 10.52EE409 pKa = 3.85RR410 pKa = 11.84DD411 pKa = 3.41MDD413 pKa = 4.06LVSTIRR419 pKa = 11.84NTSHH423 pKa = 6.87HH424 pKa = 6.45GEE426 pKa = 3.94IGATNYY432 pKa = 10.36APLMNYY438 pKa = 9.66LHH440 pKa = 7.46LDD442 pKa = 3.55TVVFRR447 pKa = 11.84SYY449 pKa = 11.62GPLVWAQKK457 pKa = 10.02NSRR460 pKa = 11.84QIFIPKK466 pKa = 10.26GLIQRR471 pKa = 11.84VAFNLVGKK479 pKa = 9.17PRR481 pKa = 11.84NRR483 pKa = 11.84DD484 pKa = 3.35TLIMCVRR491 pKa = 11.84DD492 pKa = 3.75TKK494 pKa = 11.15QEE496 pKa = 3.99LNKK499 pKa = 10.48RR500 pKa = 11.84NLEE503 pKa = 4.16LPDD506 pKa = 3.54SVRR509 pKa = 11.84AEE511 pKa = 3.82LAVFVPPLAFLLHH524 pKa = 7.12LEE526 pKa = 5.13DD527 pKa = 4.49EE528 pKa = 4.7VQSFNRR534 pKa = 11.84LVMPTNQKK542 pKa = 10.37LYY544 pKa = 10.38KK545 pKa = 9.63QLNDD549 pKa = 4.5AINLVKK555 pKa = 10.38PYY557 pKa = 10.87GCLFKK562 pKa = 11.17CFGKK566 pKa = 10.57LKK568 pKa = 9.1HH569 pKa = 5.92QEE571 pKa = 3.84SSVVVDD577 pKa = 4.91EE578 pKa = 4.59YY579 pKa = 11.63NSTRR583 pKa = 11.84TSLDD587 pKa = 3.19TVKK590 pKa = 10.91EE591 pKa = 4.13KK592 pKa = 9.77ITTGFAFLSTTAKK605 pKa = 10.74NAMKK609 pKa = 9.95KK610 pKa = 9.34QRR612 pKa = 11.84SGTYY616 pKa = 8.43FKK618 pKa = 11.12VAIEE622 pKa = 4.13EE623 pKa = 4.14PRR625 pKa = 11.84EE626 pKa = 3.8DD627 pKa = 3.87KK628 pKa = 11.36YY629 pKa = 11.95VMKK632 pKa = 10.61QVATTFSGHH641 pKa = 5.95IPIVPTKK648 pKa = 8.54TQSNTEE654 pKa = 3.58LALRR658 pKa = 11.84NRR660 pKa = 11.84VLAEE664 pKa = 3.66VTPPLEE670 pKa = 4.15GHH672 pKa = 6.86WDD674 pKa = 3.32NMADD678 pKa = 4.01RR679 pKa = 11.84FFNKK683 pKa = 9.49QFLDD687 pKa = 4.13FTQFDD692 pKa = 4.72YY693 pKa = 9.9EE694 pKa = 4.15TDD696 pKa = 3.33EE697 pKa = 5.33DD698 pKa = 4.19KK699 pKa = 11.66AFVKK703 pKa = 9.8WNSGFQPGRR712 pKa = 11.84QKK714 pKa = 10.79DD715 pKa = 3.56HH716 pKa = 5.59EE717 pKa = 4.16RR718 pKa = 11.84ARR720 pKa = 11.84INLKK724 pKa = 10.64NEE726 pKa = 4.37GIKK729 pKa = 10.48NKK731 pKa = 10.02HH732 pKa = 5.42YY733 pKa = 10.12YY734 pKa = 9.99RR735 pKa = 11.84KK736 pKa = 8.15TFMKK740 pKa = 10.43VEE742 pKa = 4.14KK743 pKa = 9.79LDD745 pKa = 3.53KK746 pKa = 10.98SSADD750 pKa = 3.22GVEE753 pKa = 4.25DD754 pKa = 4.27FNPRR758 pKa = 11.84AISGTSHH765 pKa = 6.78EE766 pKa = 5.24SNVVMGPFMTQYY778 pKa = 10.97SKK780 pKa = 11.15QLAKK784 pKa = 10.09QWNGEE789 pKa = 3.77GTFYY793 pKa = 10.07YY794 pKa = 10.44TSGATGEE801 pKa = 4.48STGKK805 pKa = 9.37WMYY808 pKa = 11.64DD809 pKa = 3.01NFTDD813 pKa = 3.52GDD815 pKa = 4.63LIVEE819 pKa = 4.4VDD821 pKa = 4.48FSAYY825 pKa = 10.55DD826 pKa = 3.48STQSRR831 pKa = 11.84DD832 pKa = 2.91SYY834 pKa = 11.15LFEE837 pKa = 5.28KK838 pKa = 10.02RR839 pKa = 11.84VLMAAGMDD847 pKa = 3.3KK848 pKa = 10.99HH849 pKa = 6.28EE850 pKa = 4.63AVCEE854 pKa = 4.26VFKK857 pKa = 10.79HH858 pKa = 4.63QSNMRR863 pKa = 11.84GFTGDD868 pKa = 3.34GIEE871 pKa = 4.03YY872 pKa = 9.92KK873 pKa = 10.66LPYY876 pKa = 9.31GRR878 pKa = 11.84NSGDD882 pKa = 3.62PNTSCGNSLLTAATTEE898 pKa = 4.16YY899 pKa = 11.28VLTKK903 pKa = 10.58VFGPGDD909 pKa = 3.67YY910 pKa = 9.98KK911 pKa = 10.77IKK913 pKa = 11.05VMGDD917 pKa = 3.4DD918 pKa = 3.33NTSIVKK924 pKa = 8.5MANANGVEE932 pKa = 3.89MDD934 pKa = 3.88EE935 pKa = 4.85VIEE938 pKa = 3.96LVKK941 pKa = 10.84KK942 pKa = 10.45EE943 pKa = 3.75FLKK946 pKa = 11.0FGFVAKK952 pKa = 10.43VKK954 pKa = 9.79IHH956 pKa = 6.45YY957 pKa = 9.52DD958 pKa = 3.16VASAEE963 pKa = 4.51FCSSAYY969 pKa = 9.11WPATINNHH977 pKa = 4.65HH978 pKa = 6.93TYY980 pKa = 11.25VMGPKK985 pKa = 9.81PGRR988 pKa = 11.84LLPKK992 pKa = 8.88MGYY995 pKa = 9.18VIKK998 pKa = 10.37DD999 pKa = 3.56LSPGEE1004 pKa = 4.06VKK1006 pKa = 10.72GMFEE1010 pKa = 5.33GYY1012 pKa = 10.89ASTCSHH1018 pKa = 6.35IPVLKK1023 pKa = 10.51QYY1025 pKa = 11.09VNHH1028 pKa = 6.92CLKK1031 pKa = 10.72QMEE1034 pKa = 4.63TIKK1037 pKa = 10.22TKK1039 pKa = 10.87NYY1041 pKa = 7.43TDD1043 pKa = 4.11KK1044 pKa = 10.5EE1045 pKa = 4.24AKK1047 pKa = 10.2YY1048 pKa = 10.75KK1049 pKa = 9.44LTNTGGAVDD1058 pKa = 4.22NDD1060 pKa = 3.18QTAAFFLQRR1069 pKa = 11.84YY1070 pKa = 7.87GLSLDD1075 pKa = 3.49MTIDD1079 pKa = 3.4SLKK1082 pKa = 9.75RR1083 pKa = 11.84TLDD1086 pKa = 3.28GSGPDD1091 pKa = 5.75DD1092 pKa = 3.58MVHH1095 pKa = 5.87WPLLEE1100 pKa = 4.32VIRR1103 pKa = 11.84HH1104 pKa = 4.71VDD1106 pKa = 2.88AA1107 pKa = 5.92

Molecular weight:
124.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1581

474

1107

790.5

87.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.958 ± 1.001

1.328 ± 0.162

5.187 ± 0.822

4.491 ± 1.159

3.732 ± 0.086

7.084 ± 0.053

2.404 ± 0.923

4.87 ± 0.738

6.641 ± 1.683

7.147 ± 0.484

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.374

5.946 ± 0.227

4.934 ± 0.326

3.732 ± 0.788

4.681 ± 0.772

7.653 ± 1.339

8.539 ± 1.564

7.021 ± 0.215

0.886 ± 0.025

4.238 ± 0.239

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski