Pedobacter ginsengisoli

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1U7K3|A0A2D1U7K3_9SPHI Alpha-L-rhamnosidase OS=Pedobacter ginsengisoli OX=363852 GN=CPT03_14400 PE=4 SV=1
MM1 pKa = 7.59IAFEE5 pKa = 5.25KK6 pKa = 10.86DD7 pKa = 3.07LSDD10 pKa = 3.69LKK12 pKa = 11.43GLIEE16 pKa = 5.13PDD18 pKa = 4.1SISPDD23 pKa = 3.58DD24 pKa = 4.95FEE26 pKa = 6.5LLDD29 pKa = 4.18SVDD32 pKa = 4.09DD33 pKa = 4.08DD34 pKa = 4.09VVRR37 pKa = 11.84ILLSSVDD44 pKa = 3.91KK45 pKa = 11.11VIKK48 pKa = 10.19CSKK51 pKa = 9.81TYY53 pKa = 11.28LLINNLDD60 pKa = 3.75EE61 pKa = 5.02LEE63 pKa = 4.17VMEE66 pKa = 4.4NEE68 pKa = 4.62EE69 pKa = 4.34YY70 pKa = 11.2NLLASDD76 pKa = 4.22NYY78 pKa = 9.72YY79 pKa = 11.11AYY81 pKa = 9.88IIDD84 pKa = 4.28WEE86 pKa = 4.54SKK88 pKa = 9.71SYY90 pKa = 11.26KK91 pKa = 10.28DD92 pKa = 4.89LLYY95 pKa = 11.07NLNAVYY101 pKa = 9.68FSISQLLYY109 pKa = 9.35HH110 pKa = 6.57TSCQIKK116 pKa = 10.2LNEE119 pKa = 3.99VEE121 pKa = 5.1IPEE124 pKa = 4.02EE125 pKa = 4.16MYY127 pKa = 11.23DD128 pKa = 3.71EE129 pKa = 5.31FLDD132 pKa = 4.35KK133 pKa = 11.42YY134 pKa = 11.31SDD136 pKa = 3.87LLRR139 pKa = 11.84GRR141 pKa = 11.84LNPADD146 pKa = 4.11KK147 pKa = 10.79NISLLYY153 pKa = 10.59DD154 pKa = 4.31LIIDD158 pKa = 4.49LNVDD162 pKa = 3.74LSDD165 pKa = 3.22IDD167 pKa = 4.06SFIEE171 pKa = 3.93

Molecular weight: 19.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1U8N5|A0A2D1U8N5_9SPHI Lipase OS=Pedobacter ginsengisoli OX=363852 GN=CPT03_16465 PE=4 SV=1
MM1 pKa = 7.36LRR3 pKa = 11.84HH4 pKa = 6.56LGTKK8 pKa = 8.36RR9 pKa = 11.84TYY11 pKa = 9.56RR12 pKa = 11.84HH13 pKa = 5.08NVKK16 pKa = 10.13LAALLCVTAGFVNAAGFLGFSVLTTNVTGHH46 pKa = 6.89AALFAEE52 pKa = 5.67RR53 pKa = 11.84IAMQDD58 pKa = 2.73WQTARR63 pKa = 11.84VVALWMFLFLAGAFISSLTVSLIGRR88 pKa = 11.84NQQFSYY94 pKa = 10.57VIPILMEE101 pKa = 4.3LLVLLIVGLAGYY113 pKa = 9.72RR114 pKa = 11.84YY115 pKa = 10.17DD116 pKa = 3.52GSLVARR122 pKa = 11.84EE123 pKa = 4.33LFAGSLLFVMGLQNALVSMISGSVVRR149 pKa = 11.84TTHH152 pKa = 5.27LTGTFTDD159 pKa = 4.44LGIEE163 pKa = 4.31LAQIIQKK170 pKa = 9.98GNRR173 pKa = 11.84KK174 pKa = 8.78NAVLRR179 pKa = 11.84SRR181 pKa = 11.84IKK183 pKa = 9.5MRR185 pKa = 11.84AAIISFFLFGALLGAYY201 pKa = 9.01LFNVFQFHH209 pKa = 7.21AFLFPVAVLLYY220 pKa = 9.77TLFYY224 pKa = 10.68DD225 pKa = 4.57IFRR228 pKa = 11.84ISIKK232 pKa = 10.2RR233 pKa = 11.84FYY235 pKa = 11.08RR236 pKa = 11.84NLQLPWKK243 pKa = 9.6NHH245 pKa = 4.17

Molecular weight: 27.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4393

0

4393

1605044

36

3666

365.4

41.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.073 ± 0.041

0.753 ± 0.01

5.36 ± 0.023

5.729 ± 0.036

4.95 ± 0.03

6.825 ± 0.037

1.725 ± 0.017

7.519 ± 0.035

7.611 ± 0.035

9.489 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.017

5.978 ± 0.03

3.717 ± 0.019

3.54 ± 0.021

3.685 ± 0.022

6.562 ± 0.026

5.705 ± 0.052

6.234 ± 0.027

1.204 ± 0.015

4.127 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski