Megavirus lba

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; Mimivirus; unclassified Mimivirus; Megavirus chiliensis

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7Y376|L7Y376_9VIRU Uncharacterized protein OS=Megavirus lba OX=1235314 GN=LBA_00244 PE=4 SV=1
MM1 pKa = 7.38FLHH4 pKa = 7.08PSYY7 pKa = 11.25NDD9 pKa = 3.14NLFTTKK15 pKa = 10.47CGMCDD20 pKa = 3.05SDD22 pKa = 5.05AKK24 pKa = 10.79AKK26 pKa = 10.03YY27 pKa = 9.73RR28 pKa = 11.84SGTVTYY34 pKa = 10.23YY35 pKa = 10.67RR36 pKa = 11.84NKK38 pKa = 10.25NSSIHH43 pKa = 5.53NFCSKK48 pKa = 10.68EE49 pKa = 4.17CMDD52 pKa = 4.19KK53 pKa = 11.07YY54 pKa = 11.43NRR56 pKa = 11.84TEE58 pKa = 3.87KK59 pKa = 10.66CWFCSYY65 pKa = 11.05YY66 pKa = 11.07GDD68 pKa = 5.25LVPTDD73 pKa = 3.88SGFMVCTSTEE83 pKa = 3.92YY84 pKa = 10.45WDD86 pKa = 5.07FSCLDD91 pKa = 3.41KK92 pKa = 11.78YY93 pKa = 11.13NIRR96 pKa = 11.84KK97 pKa = 8.01NHH99 pKa = 5.5NLILEE104 pKa = 4.37WNPFCDD110 pKa = 4.42DD111 pKa = 4.35DD112 pKa = 5.57YY113 pKa = 11.71EE114 pKa = 4.44ILSKK118 pKa = 10.97SDD120 pKa = 4.11IIPDD124 pKa = 3.51EE125 pKa = 4.18YY126 pKa = 11.36KK127 pKa = 10.65EE128 pKa = 3.94YY129 pKa = 10.97LVNNNNDD136 pKa = 3.38NDD138 pKa = 4.05NNDD141 pKa = 4.41DD142 pKa = 4.21NNDD145 pKa = 3.7NNDD148 pKa = 4.93DD149 pKa = 4.28NNDD152 pKa = 4.38DD153 pKa = 4.9NNDD156 pKa = 4.13DD157 pKa = 4.6NNDD160 pKa = 4.01DD161 pKa = 4.54NDD163 pKa = 5.58DD164 pKa = 4.27NNDD167 pKa = 3.95DD168 pKa = 4.73NNDD171 pKa = 3.53NNDD174 pKa = 4.67DD175 pKa = 4.66DD176 pKa = 6.37NIDD179 pKa = 4.27NNDD182 pKa = 3.53DD183 pKa = 4.54DD184 pKa = 3.9II185 pKa = 6.85

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7Y2Y9|L7Y2Y9_9VIRU Putative ankyrin repeat protein OS=Megavirus lba OX=1235314 GN=LBA_00169 PE=4 SV=1
MM1 pKa = 7.25TPNIIIIVDD10 pKa = 3.34NKK12 pKa = 10.34MEE14 pKa = 4.18NTNALEE20 pKa = 3.83INAAITLVTLRR31 pKa = 11.84RR32 pKa = 11.84NSVNFDD38 pKa = 3.36TRR40 pKa = 11.84KK41 pKa = 8.63KK42 pKa = 8.77TPQNNGNKK50 pKa = 9.33IFRR53 pKa = 11.84SRR55 pKa = 11.84QPNKK59 pKa = 9.96YY60 pKa = 9.63QDD62 pKa = 3.78VLTFIYY68 pKa = 9.69QQHH71 pKa = 7.0KK72 pKa = 10.15YY73 pKa = 8.94PDD75 pKa = 3.19PHH77 pKa = 6.54MYY79 pKa = 10.38QNLSIILGLSIRR91 pKa = 11.84QIQIWFQNRR100 pKa = 11.84RR101 pKa = 11.84ARR103 pKa = 11.84DD104 pKa = 3.48SKK106 pKa = 11.21KK107 pKa = 10.32NRR109 pKa = 11.84LSNKK113 pKa = 9.35LGKK116 pKa = 10.07

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1176

0

1176

365887

30

2907

311.1

36.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.062 ± 0.053

1.941 ± 0.051

6.629 ± 0.064

5.062 ± 0.065

4.478 ± 0.06

4.525 ± 0.201

2.203 ± 0.044

11.042 ± 0.09

8.792 ± 0.092

8.03 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.035

10.024 ± 0.115

3.137 ± 0.065

3.173 ± 0.049

2.907 ± 0.056

6.759 ± 0.075

5.021 ± 0.053

4.325 ± 0.056

0.829 ± 0.026

5.786 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski