Amino acid dipepetide frequency for Megavirus lba

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.317AlaAla: 1.317 ± 0.09
0.907AlaCys: 0.907 ± 0.066
1.82AlaAsp: 1.82 ± 0.081
1.509AlaGlu: 1.509 ± 0.076
1.358AlaPhe: 1.358 ± 0.067
1.372AlaGly: 1.372 ± 0.08
0.571AlaHis: 0.571 ± 0.04
3.269AlaIle: 3.269 ± 0.118
2.613AlaLys: 2.613 ± 0.105
2.411AlaLeu: 2.411 ± 0.092
0.76AlaMet: 0.76 ± 0.051
2.646AlaAsn: 2.646 ± 0.128
0.883AlaPro: 0.883 ± 0.068
0.978AlaGln: 0.978 ± 0.056
1.052AlaArg: 1.052 ± 0.065
2.443AlaSer: 2.443 ± 0.114
1.528AlaThr: 1.528 ± 0.059
1.558AlaVal: 1.558 ± 0.08
0.208AlaTrp: 0.208 ± 0.023
1.413AlaTyr: 1.413 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.046
0.437CysCys: 0.437 ± 0.052
1.427CysAsp: 1.427 ± 0.068
0.905CysGlu: 0.905 ± 0.059
0.793CysPhe: 0.793 ± 0.048
1.145CysGly: 1.145 ± 0.063
0.456CysHis: 0.456 ± 0.036
2.878CysIle: 2.878 ± 0.219
1.405CysLys: 1.405 ± 0.079
1.358CysLeu: 1.358 ± 0.07
0.413CysMet: 0.413 ± 0.037
1.435CysAsn: 1.435 ± 0.102
0.719CysPro: 0.719 ± 0.068
0.804CysGln: 0.804 ± 0.054
0.768CysArg: 0.768 ± 0.052
1.011CysSer: 1.011 ± 0.05
0.793CysThr: 0.793 ± 0.049
0.916CysVal: 0.916 ± 0.059
0.178CysTrp: 0.178 ± 0.026
0.894CysTyr: 0.894 ± 0.047
0.0CysXaa: 0.0 ± 0.0
Asp
1.828AspAla: 1.828 ± 0.087
1.593AspCys: 1.593 ± 0.131
4.463AspAsp: 4.463 ± 0.211
3.116AspGlu: 3.116 ± 0.106
3.272AspPhe: 3.272 ± 0.102
2.173AspGly: 2.173 ± 0.095
1.315AspHis: 1.315 ± 0.065
8.934AspIle: 8.934 ± 0.207
6.305AspLys: 6.305 ± 0.292
5.412AspLeu: 5.412 ± 0.159
1.421AspMet: 1.421 ± 0.057
6.568AspAsn: 6.568 ± 0.203
1.826AspPro: 1.826 ± 0.085
1.818AspGln: 1.818 ± 0.088
1.604AspArg: 1.604 ± 0.071
4.029AspSer: 4.029 ± 0.156
2.954AspThr: 2.954 ± 0.11
3.077AspVal: 3.077 ± 0.108
0.56AspTrp: 0.56 ± 0.046
4.026AspTyr: 4.026 ± 0.099
0.0AspXaa: 0.0 ± 0.0
Glu
1.361GluAla: 1.361 ± 0.076
1.0GluCys: 1.0 ± 0.064
2.066GluAsp: 2.066 ± 0.089
2.197GluGlu: 2.197 ± 0.117
2.678GluPhe: 2.678 ± 0.085
1.189GluGly: 1.189 ± 0.068
0.883GluHis: 0.883 ± 0.05
6.212GluIle: 6.212 ± 0.17
5.529GluLys: 5.529 ± 0.264
4.838GluLeu: 4.838 ± 0.128
1.14GluMet: 1.14 ± 0.05
4.89GluAsn: 4.89 ± 0.129
1.306GluPro: 1.306 ± 0.102
1.353GluGln: 1.353 ± 0.073
1.449GluArg: 1.449 ± 0.074
3.952GluSer: 3.952 ± 0.185
2.758GluThr: 2.758 ± 0.099
1.473GluVal: 1.473 ± 0.1
0.459GluTrp: 0.459 ± 0.037
3.884GluTyr: 3.884 ± 0.106
0.0GluXaa: 0.0 ± 0.0
Phe
1.257PheAla: 1.257 ± 0.069
0.749PheCys: 0.749 ± 0.051
3.75PheAsp: 3.75 ± 0.127
2.441PheGlu: 2.441 ± 0.088
1.815PhePhe: 1.815 ± 0.069
3.802PheGly: 3.802 ± 0.233
0.782PheHis: 0.782 ± 0.053
4.261PheIle: 4.261 ± 0.12
3.233PheLys: 3.233 ± 0.101
3.045PheLeu: 3.045 ± 0.097
1.235PheMet: 1.235 ± 0.067
5.461PheAsn: 5.461 ± 0.24
1.219PhePro: 1.219 ± 0.058
1.123PheGln: 1.123 ± 0.058
1.271PheArg: 1.271 ± 0.062
2.618PheSer: 2.618 ± 0.084
2.031PheThr: 2.031 ± 0.079
2.227PheVal: 2.227 ± 0.083
0.257PheTrp: 0.257 ± 0.024
2.2PheTyr: 2.2 ± 0.085
0.0PheXaa: 0.0 ± 0.0
Gly
2.124GlyAla: 2.124 ± 0.118
1.197GlyCys: 1.197 ± 0.081
4.07GlyAsp: 4.07 ± 0.657
2.711GlyGlu: 2.711 ± 0.384
2.066GlyPhe: 2.066 ± 0.078
2.643GlyGly: 2.643 ± 0.243
1.181GlyHis: 1.181 ± 0.068
3.671GlyIle: 3.671 ± 0.109
3.211GlyLys: 3.211 ± 0.102
3.528GlyLeu: 3.528 ± 0.193
0.872GlyMet: 0.872 ± 0.117
3.359GlyAsn: 3.359 ± 0.182
1.241GlyPro: 1.241 ± 0.159
1.317GlyGln: 1.317 ± 0.073
1.331GlyArg: 1.331 ± 0.071
3.282GlySer: 3.282 ± 0.2
2.26GlyThr: 2.26 ± 0.106
1.793GlyVal: 1.793 ± 0.089
0.607GlyTrp: 0.607 ± 0.055
2.782GlyTyr: 2.782 ± 0.121
0.0GlyXaa: 0.0 ± 0.0
His
0.623HisAla: 0.623 ± 0.041
0.454HisCys: 0.454 ± 0.038
1.405HisAsp: 1.405 ± 0.058
1.011HisGlu: 1.011 ± 0.059
1.006HisPhe: 1.006 ± 0.061
1.025HisGly: 1.025 ± 0.054
0.664HisHis: 0.664 ± 0.053
2.299HisIle: 2.299 ± 0.078
1.648HisLys: 1.648 ± 0.07
3.272HisLeu: 3.272 ± 0.221
0.489HisMet: 0.489 ± 0.037
1.951HisAsn: 1.951 ± 0.076
0.705HisPro: 0.705 ± 0.049
0.588HisGln: 0.588 ± 0.039
0.683HisArg: 0.683 ± 0.052
1.041HisSer: 1.041 ± 0.062
0.957HisThr: 0.957 ± 0.048
0.869HisVal: 0.869 ± 0.043
0.145HisTrp: 0.145 ± 0.02
1.197HisTyr: 1.197 ± 0.066
0.0HisXaa: 0.0 ± 0.0
Ile
3.102IleAla: 3.102 ± 0.106
1.774IleCys: 1.774 ± 0.077
8.024IleAsp: 8.024 ± 0.187
5.813IleGlu: 5.813 ± 0.161
4.603IlePhe: 4.603 ± 0.127
4.004IleGly: 4.004 ± 0.2
2.249IleHis: 2.249 ± 0.078
12.313IleIle: 12.313 ± 0.267
11.115IleLys: 11.115 ± 0.263
8.117IleLeu: 8.117 ± 0.185
2.687IleMet: 2.687 ± 0.098
11.597IleAsn: 11.597 ± 0.287
5.234IlePro: 5.234 ± 0.262
2.935IleGln: 2.935 ± 0.107
3.069IleArg: 3.069 ± 0.106
7.002IleSer: 7.002 ± 0.164
5.286IleThr: 5.286 ± 0.143
4.835IleVal: 4.835 ± 0.144
0.782IleTrp: 0.782 ± 0.05
5.876IleTyr: 5.876 ± 0.144
0.0IleXaa: 0.0 ± 0.0
Lys
1.722LysAla: 1.722 ± 0.092
1.705LysCys: 1.705 ± 0.08
3.785LysAsp: 3.785 ± 0.146
3.416LysGlu: 3.416 ± 0.125
4.122LysPhe: 4.122 ± 0.122
4.466LysGly: 4.466 ± 0.83
1.782LysHis: 1.782 ± 0.082
10.651LysIle: 10.651 ± 0.223
8.18LysLys: 8.18 ± 0.241
7.822LysLeu: 7.822 ± 0.192
2.11LysMet: 2.11 ± 0.078
9.64LysAsn: 9.64 ± 0.204
2.424LysPro: 2.424 ± 0.102
2.596LysGln: 2.596 ± 0.099
2.126LysArg: 2.126 ± 0.091
6.018LysSer: 6.018 ± 0.17
4.346LysThr: 4.346 ± 0.142
2.299LysVal: 2.299 ± 0.094
0.809LysTrp: 0.809 ± 0.045
7.896LysTyr: 7.896 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
2.897LeuAla: 2.897 ± 0.096
1.208LeuCys: 1.208 ± 0.059
5.688LeuAsp: 5.688 ± 0.152
5.07LeuGlu: 5.07 ± 0.17
3.438LeuPhe: 3.438 ± 0.112
3.332LeuGly: 3.332 ± 0.173
1.498LeuHis: 1.498 ± 0.072
7.839LeuIle: 7.839 ± 0.187
7.03LeuLys: 7.03 ± 0.177
7.398LeuLeu: 7.398 ± 0.218
1.878LeuMet: 1.878 ± 0.093
6.677LeuAsn: 6.677 ± 0.155
2.769LeuPro: 2.769 ± 0.097
2.405LeuGln: 2.405 ± 0.092
2.353LeuArg: 2.353 ± 0.091
6.19LeuSer: 6.19 ± 0.17
4.469LeuThr: 4.469 ± 0.164
3.96LeuVal: 3.96 ± 0.116
0.478LeuTrp: 0.478 ± 0.038
3.725LeuTyr: 3.725 ± 0.113
0.0LeuXaa: 0.0 ± 0.0
Met
0.888MetAla: 0.888 ± 0.05
0.377MetCys: 0.377 ± 0.028
1.703MetAsp: 1.703 ± 0.081
1.353MetGlu: 1.353 ± 0.07
0.91MetPhe: 0.91 ± 0.049
0.855MetGly: 0.855 ± 0.082
0.451MetHis: 0.451 ± 0.038
2.35MetIle: 2.35 ± 0.093
1.615MetLys: 1.615 ± 0.073
1.566MetLeu: 1.566 ± 0.071
0.533MetMet: 0.533 ± 0.035
2.184MetAsn: 2.184 ± 0.104
0.637MetPro: 0.637 ± 0.047
0.648MetGln: 0.648 ± 0.048
0.702MetArg: 0.702 ± 0.042
2.197MetSer: 2.197 ± 0.092
1.449MetThr: 1.449 ± 0.072
0.948MetVal: 0.948 ± 0.049
0.175MetTrp: 0.175 ± 0.021
1.233MetTyr: 1.233 ± 0.067
0.0MetXaa: 0.0 ± 0.0
Asn
2.501AsnAla: 2.501 ± 0.102
1.708AsnCys: 1.708 ± 0.096
6.275AsnAsp: 6.275 ± 0.181
4.149AsnGlu: 4.149 ± 0.136
4.04AsnPhe: 4.04 ± 0.122
4.255AsnGly: 4.255 ± 0.214
2.151AsnHis: 2.151 ± 0.091
13.406AsnIle: 13.406 ± 0.289
8.893AsnLys: 8.893 ± 0.207
7.237AsnLeu: 7.237 ± 0.176
2.449AsnMet: 2.449 ± 0.089
12.307AsnAsn: 12.307 ± 0.351
2.711AsnPro: 2.711 ± 0.128
4.264AsnGln: 4.264 ± 0.211
2.676AsnArg: 2.676 ± 0.089
6.442AsnSer: 6.442 ± 0.209
4.717AsnThr: 4.717 ± 0.137
3.799AsnVal: 3.799 ± 0.119
0.795AsnTrp: 0.795 ± 0.066
5.461AsnTyr: 5.461 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
1.03ProAla: 1.03 ± 0.066
0.634ProCys: 0.634 ± 0.097
2.151ProAsp: 2.151 ± 0.076
1.979ProGlu: 1.979 ± 0.1
1.429ProPhe: 1.429 ± 0.128
1.361ProGly: 1.361 ± 0.068
0.552ProHis: 0.552 ± 0.039
3.348ProIle: 3.348 ± 0.119
2.667ProLys: 2.667 ± 0.097
2.042ProLeu: 2.042 ± 0.114
0.629ProMet: 0.629 ± 0.064
4.261ProAsn: 4.261 ± 0.208
0.916ProPro: 0.916 ± 0.09
0.839ProGln: 0.839 ± 0.062
0.787ProArg: 0.787 ± 0.052
1.878ProSer: 1.878 ± 0.086
1.514ProThr: 1.514 ± 0.08
1.686ProVal: 1.686 ± 0.103
0.197ProTrp: 0.197 ± 0.025
1.47ProTyr: 1.47 ± 0.078
0.0ProXaa: 0.0 ± 0.0
Gln
0.916GlnAla: 0.916 ± 0.058
0.588GlnCys: 0.588 ± 0.05
2.104GlnAsp: 2.104 ± 0.089
1.768GlnGlu: 1.768 ± 0.088
1.271GlnPhe: 1.271 ± 0.061
1.091GlnGly: 1.091 ± 0.079
0.615GlnHis: 0.615 ± 0.039
3.351GlnIle: 3.351 ± 0.103
2.758GlnLys: 2.758 ± 0.108
2.651GlnLeu: 2.651 ± 0.083
0.825GlnMet: 0.825 ± 0.058
3.542GlnAsn: 3.542 ± 0.135
0.937GlnPro: 0.937 ± 0.075
1.451GlnGln: 1.451 ± 0.137
0.853GlnArg: 0.853 ± 0.06
2.17GlnSer: 2.17 ± 0.091
1.301GlnThr: 1.301 ± 0.071
1.194GlnVal: 1.194 ± 0.058
0.26GlnTrp: 0.26 ± 0.026
2.08GlnTyr: 2.08 ± 0.088
0.0GlnXaa: 0.0 ± 0.0
Arg
0.97ArgAla: 0.97 ± 0.058
0.675ArgCys: 0.675 ± 0.05
1.88ArgAsp: 1.88 ± 0.089
1.774ArgGlu: 1.774 ± 0.087
1.481ArgPhe: 1.481 ± 0.066
1.435ArgGly: 1.435 ± 0.081
0.65ArgHis: 0.65 ± 0.044
2.766ArgIle: 2.766 ± 0.079
2.424ArgLys: 2.424 ± 0.081
2.309ArgLeu: 2.309 ± 0.076
0.533ArgMet: 0.533 ± 0.044
2.602ArgAsn: 2.602 ± 0.093
0.872ArgPro: 0.872 ± 0.059
1.052ArgGln: 1.052 ± 0.061
1.104ArgArg: 1.104 ± 0.071
1.924ArgSer: 1.924 ± 0.139
1.276ArgThr: 1.276 ± 0.056
1.334ArgVal: 1.334 ± 0.063
0.208ArgTrp: 0.208 ± 0.025
1.801ArgTyr: 1.801 ± 0.075
0.0ArgXaa: 0.0 ± 0.0
Ser
2.11SerAla: 2.11 ± 0.096
1.285SerCys: 1.285 ± 0.069
5.239SerAsp: 5.239 ± 0.157
3.758SerGlu: 3.758 ± 0.155
2.443SerPhe: 2.443 ± 0.076
3.949SerGly: 3.949 ± 0.202
1.274SerHis: 1.274 ± 0.06
6.647SerIle: 6.647 ± 0.181
6.308SerLys: 6.308 ± 0.181
4.324SerLeu: 4.324 ± 0.109
1.462SerMet: 1.462 ± 0.069
6.464SerAsn: 6.464 ± 0.201
2.05SerPro: 2.05 ± 0.207
2.361SerGln: 2.361 ± 0.086
2.258SerArg: 2.258 ± 0.115
4.706SerSer: 4.706 ± 0.197
3.239SerThr: 3.239 ± 0.118
4.1SerVal: 4.1 ± 0.212
0.544SerTrp: 0.544 ± 0.038
3.069SerTyr: 3.069 ± 0.1
0.0SerXaa: 0.0 ± 0.0
Thr
1.618ThrAla: 1.618 ± 0.077
0.976ThrCys: 0.976 ± 0.055
2.976ThrAsp: 2.976 ± 0.106
2.364ThrGlu: 2.364 ± 0.097
2.643ThrPhe: 2.643 ± 0.151
2.46ThrGly: 2.46 ± 0.116
2.394ThrHis: 2.394 ± 0.212
5.433ThrIle: 5.433 ± 0.133
4.187ThrLys: 4.187 ± 0.134
3.362ThrLeu: 3.362 ± 0.097
1.047ThrMet: 1.047 ± 0.059
5.062ThrAsn: 5.062 ± 0.138
1.697ThrPro: 1.697 ± 0.101
1.462ThrGln: 1.462 ± 0.07
1.599ThrArg: 1.599 ± 0.067
3.37ThrSer: 3.37 ± 0.123
2.602ThrThr: 2.602 ± 0.138
2.072ThrVal: 2.072 ± 0.078
0.418ThrTrp: 0.418 ± 0.034
2.471ThrTyr: 2.471 ± 0.073
0.0ThrXaa: 0.0 ± 0.0
Val
1.522ValAla: 1.522 ± 0.078
0.79ValCys: 0.79 ± 0.055
2.976ValAsp: 2.976 ± 0.093
2.37ValGlu: 2.37 ± 0.102
1.804ValPhe: 1.804 ± 0.067
1.618ValGly: 1.618 ± 0.076
0.743ValHis: 0.743 ± 0.052
4.19ValIle: 4.19 ± 0.136
4.146ValLys: 4.146 ± 0.122
3.176ValLeu: 3.176 ± 0.111
0.913ValMet: 0.913 ± 0.066
3.671ValAsn: 3.671 ± 0.106
1.465ValPro: 1.465 ± 0.067
1.213ValGln: 1.213 ± 0.069
1.252ValArg: 1.252 ± 0.068
3.105ValSer: 3.105 ± 0.113
3.493ValThr: 3.493 ± 0.2
2.225ValVal: 2.225 ± 0.096
0.358ValTrp: 0.358 ± 0.031
2.219ValTyr: 2.219 ± 0.089
0.0ValXaa: 0.0 ± 0.0
Trp
0.309TrpAla: 0.309 ± 0.031
0.224TrpCys: 0.224 ± 0.025
0.38TrpAsp: 0.38 ± 0.029
0.276TrpGlu: 0.276 ± 0.029
0.443TrpPhe: 0.443 ± 0.031
0.224TrpGly: 0.224 ± 0.029
0.15TrpHis: 0.15 ± 0.017
1.028TrpIle: 1.028 ± 0.062
0.727TrpLys: 0.727 ± 0.046
0.631TrpLeu: 0.631 ± 0.058
0.148TrpMet: 0.148 ± 0.022
0.85TrpAsn: 0.85 ± 0.054
0.142TrpPro: 0.142 ± 0.024
0.219TrpGln: 0.219 ± 0.023
0.295TrpArg: 0.295 ± 0.035
0.544TrpSer: 0.544 ± 0.042
0.47TrpThr: 0.47 ± 0.038
0.292TrpVal: 0.292 ± 0.027
0.418TrpTrp: 0.418 ± 0.101
0.517TrpTyr: 0.517 ± 0.048
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.848TyrAla: 1.848 ± 0.077
1.126TyrCys: 1.126 ± 0.06
4.111TyrAsp: 4.111 ± 0.103
2.637TyrGlu: 2.637 ± 0.089
3.162TyrPhe: 3.162 ± 0.106
2.848TyrGly: 2.848 ± 0.105
1.67TyrHis: 1.67 ± 0.073
5.573TyrIle: 5.573 ± 0.131
3.919TyrLys: 3.919 ± 0.104
5.802TyrLeu: 5.802 ± 0.153
1.23TyrMet: 1.23 ± 0.055
5.073TyrAsn: 5.073 ± 0.125
1.618TyrPro: 1.618 ± 0.073
2.299TyrGln: 2.299 ± 0.088
1.839TyrArg: 1.839 ± 0.078
3.665TyrSer: 3.665 ± 0.1
2.771TyrThr: 2.771 ± 0.092
2.591TyrVal: 2.591 ± 0.086
0.432TyrTrp: 0.432 ± 0.036
3.649TyrTyr: 3.649 ± 0.108
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1176 proteins (365887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski