Metallibacterium scheffleri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Metallibacterium

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S3KE79|A0A4S3KE79_9GAMM 2-C-methyl-D-erythritol 2 4-cyclodiphosphate synthase OS=Metallibacterium scheffleri OX=993689 GN=ispF PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84NLKK5 pKa = 10.54RR6 pKa = 11.84NLLTLAILAGAGSIAPAAFAQEE28 pKa = 4.67GPSCGGTCTVNTTVNYY44 pKa = 9.58TLDD47 pKa = 3.42QQDD50 pKa = 3.78WIRR53 pKa = 11.84NYY55 pKa = 10.84LLNASAYY62 pKa = 9.54IYY64 pKa = 11.11GDD66 pKa = 3.14VQYY69 pKa = 11.27YY70 pKa = 10.4EE71 pKa = 4.57NVDD74 pKa = 3.35INTDD78 pKa = 3.05TSIRR82 pKa = 11.84RR83 pKa = 11.84VRR85 pKa = 11.84MNKK88 pKa = 9.83DD89 pKa = 3.04LSLLTDD95 pKa = 3.38VSITGNPTVSGAINTDD111 pKa = 2.93SAAIAVVDD119 pKa = 3.82NRR121 pKa = 11.84QHH123 pKa = 6.27NVGNNGEE130 pKa = 4.25NYY132 pKa = 10.55LLANDD137 pKa = 4.88ASIADD142 pKa = 4.13DD143 pKa = 3.83AAQNAQGNIGLNVAAGDD160 pKa = 4.0NNQQHH165 pKa = 5.96NAAALAAADD174 pKa = 3.69ASFAFTMADD183 pKa = 2.99SEE185 pKa = 4.94VFVEE189 pKa = 4.78QVNAANTTGNFGQTNMANLGGNALQNATGNIGVNIAAGNNNQQQNNLVASTGTNSMATASVNSKK253 pKa = 10.19QDD255 pKa = 2.95SGGNYY260 pKa = 8.22TLNEE264 pKa = 4.77GYY266 pKa = 7.94TQQFVDD272 pKa = 5.94LITINLTGTVGGSGEE287 pKa = 4.36GGQVMGTSIGTSDD300 pKa = 3.02QRR302 pKa = 11.84YY303 pKa = 10.12NMFPDD308 pKa = 3.01NWGVAQGEE316 pKa = 4.93TPTSAHH322 pKa = 6.24PTNGPWLGHH331 pKa = 5.57TDD333 pKa = 3.44WDD335 pKa = 4.16TSTQGTDD342 pKa = 2.76INGNPIVRR350 pKa = 11.84TPGNDD355 pKa = 3.13PMDD358 pKa = 4.69PSNDD362 pKa = 3.42GGSLVMNNNDD372 pKa = 3.89EE373 pKa = 4.24ITLEE377 pKa = 4.01EE378 pKa = 4.08MSAALSGQIVDD389 pKa = 3.61YY390 pKa = 10.95SYY392 pKa = 11.38VAVMSTNTASLSGSALQNAQGNIGVNIASGTGNQQANSLALAVTQASSGGGSSGGGAEE450 pKa = 3.97

Molecular weight: 46.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S3KS08|A0A4S3KS08_9GAMM Uncharacterized protein OS=Metallibacterium scheffleri OX=993689 GN=B1806_01590 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.06LKK11 pKa = 10.38RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATADD26 pKa = 3.26GRR28 pKa = 11.84KK29 pKa = 9.02ILSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.96GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LIPP44 pKa = 4.02

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3178

0

3178

1048861

32

2883

330.0

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.144 ± 0.071

0.93 ± 0.014

5.256 ± 0.031

4.635 ± 0.038

3.221 ± 0.025

8.15 ± 0.042

2.628 ± 0.023

4.216 ± 0.029

2.383 ± 0.033

11.725 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.021

2.358 ± 0.028

5.402 ± 0.029

4.02 ± 0.028

7.883 ± 0.046

5.013 ± 0.034

4.832 ± 0.037

7.099 ± 0.032

1.578 ± 0.019

2.333 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski