Geodermatophilus sp. DSM 44513
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4421 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S4ZHC8|A0A5S4ZHC8_9ACTN Osmoprotectant transport system permease protein OS=Geodermatophilus sp. DSM 44513 OX=1528104 GN=JD83_02292 PE=3 SV=1
MM1 pKa = 6.91 TRR3 pKa = 11.84 SSRR6 pKa = 11.84 RR7 pKa = 11.84 LTRR10 pKa = 11.84 LIATATAVSALAACGGSDD28 pKa = 3.62 GGGAGGGGGEE38 pKa = 4.62 NEE40 pKa = 4.53 ASDD43 pKa = 3.69 TGITADD49 pKa = 4.02 AIKK52 pKa = 10.41 IGAHH56 pKa = 5.39 YY57 pKa = 9.82 PLTGVAAPGYY67 pKa = 9.3 SEE69 pKa = 4.89 IPTGVDD75 pKa = 3.32 CYY77 pKa = 11.4 FDD79 pKa = 3.69 FVNAAGGVNGRR90 pKa = 11.84 QIDD93 pKa = 3.82 YY94 pKa = 10.62 VYY96 pKa = 10.95 RR97 pKa = 11.84 DD98 pKa = 3.69 DD99 pKa = 6.02 AYY101 pKa = 11.7 NPTNTSAVVNEE112 pKa = 4.79 LVLQDD117 pKa = 3.99 EE118 pKa = 5.2 VFMLMGGLGTPTHH131 pKa = 6.06 SAVLDD136 pKa = 3.71 FLNGEE141 pKa = 4.31 GVPDD145 pKa = 4.14 LFVASGSLLWDD156 pKa = 3.56 NPEE159 pKa = 3.96 EE160 pKa = 4.09 NPYY163 pKa = 9.44 TFGWQPDD170 pKa = 3.75 YY171 pKa = 11.01 EE172 pKa = 4.39 IEE174 pKa = 4.21 GKK176 pKa = 10.95 VIGQYY181 pKa = 9.67 IAEE184 pKa = 4.65 NFPEE188 pKa = 4.05 ARR190 pKa = 11.84 VGLFLQDD197 pKa = 4.18 DD198 pKa = 4.27 DD199 pKa = 4.79 FGEE202 pKa = 4.55 DD203 pKa = 3.12 GEE205 pKa = 4.67 AGVRR209 pKa = 11.84 QFIDD213 pKa = 3.6 DD214 pKa = 3.89 QIVAAEE220 pKa = 4.33 RR221 pKa = 11.84 YY222 pKa = 9.4 VPGNTDD228 pKa = 3.09 VGPQLAALQAAGADD242 pKa = 3.51 FVVGFNVPSYY252 pKa = 9.64 TALSQLTALRR262 pKa = 11.84 LGYY265 pKa = 10.24 DD266 pKa = 3.55 PQWFYY271 pKa = 11.99 SNVGSDD277 pKa = 3.53 PALVGSLLANFSQGQVSDD295 pKa = 4.04 ASLLDD300 pKa = 3.82 GALTTEE306 pKa = 4.29 YY307 pKa = 10.58 LAGVDD312 pKa = 4.0 TPDD315 pKa = 5.09 DD316 pKa = 3.57 PWVQLWQRR324 pKa = 11.84 VWDD327 pKa = 3.79 EE328 pKa = 4.44 CEE330 pKa = 3.93 SAEE333 pKa = 5.35 GEE335 pKa = 4.13 LTNYY339 pKa = 9.37 RR340 pKa = 11.84 VYY342 pKa = 11.49 GMAQAYY348 pKa = 6.17 TTIQALQAAGQNPTRR363 pKa = 11.84 DD364 pKa = 3.65 GLVEE368 pKa = 3.99 AVEE371 pKa = 3.91 QAGAEE376 pKa = 4.17 FEE378 pKa = 4.6 GPALAPYY385 pKa = 9.66 RR386 pKa = 11.84 YY387 pKa = 9.47 SAEE390 pKa = 3.64 RR391 pKa = 11.84 HH392 pKa = 5.55 AGVGGVKK399 pKa = 9.77 IARR402 pKa = 11.84 ITGTSAEE409 pKa = 4.58 DD410 pKa = 3.38 LTPVLVTDD418 pKa = 4.32 NGDD421 pKa = 3.48 AAIEE425 pKa = 4.05 EE426 pKa = 4.49 SDD428 pKa = 4.82 AEE430 pKa = 4.31 PTTPPEE436 pKa = 3.95 SGIPDD441 pKa = 3.72 VQPVGG446 pKa = 3.4
Molecular weight: 46.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.049
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A5S4ZIL3|A0A5S4ZIL3_9ACTN Uncharacterized protein OS=Geodermatophilus sp. DSM 44513 OX=1528104 GN=JD83_02963 PE=4 SV=1
MM1 pKa = 7.35 GPAGRR6 pKa = 11.84 GRR8 pKa = 11.84 SAGRR12 pKa = 11.84 AAGDD16 pKa = 3.32 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 GARR22 pKa = 11.84 AAGRR26 pKa = 11.84 GPAARR31 pKa = 11.84 PGRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 HH37 pKa = 5.52 HH38 pKa = 5.99 GRR40 pKa = 11.84 RR41 pKa = 11.84 PGARR45 pKa = 11.84 RR46 pKa = 11.84 GLPPRR51 pKa = 11.84 RR52 pKa = 11.84 AGPPDD57 pKa = 3.02 GRR59 pKa = 11.84 LRR61 pKa = 11.84 GQRR64 pKa = 11.84 GDD66 pKa = 3.35 PAGRR70 pKa = 11.84 RR71 pKa = 11.84 PRR73 pKa = 11.84 AAAPPGGRR81 pKa = 11.84 PAGAGAGRR89 pKa = 11.84 GRR91 pKa = 11.84 GAGRR95 pKa = 11.84 LRR97 pKa = 11.84 GAGPAHH103 pKa = 6.76 RR104 pKa = 11.84 QRR106 pKa = 11.84 GAGGGHH112 pKa = 6.42 GCGHH116 pKa = 7.42 AAGTGHH122 pKa = 6.99 RR123 pKa = 11.84 PVAGRR128 pKa = 11.84 GAGPGRR134 pKa = 11.84 GRR136 pKa = 11.84 GRR138 pKa = 11.84 PAARR142 pKa = 11.84 RR143 pKa = 11.84 PAPGARR149 pKa = 11.84 RR150 pKa = 11.84 RR151 pKa = 11.84 LRR153 pKa = 11.84 ALRR156 pKa = 11.84 RR157 pKa = 11.84 RR158 pKa = 11.84 HH159 pKa = 5.38 RR160 pKa = 11.84 RR161 pKa = 11.84 HH162 pKa = 5.77 RR163 pKa = 11.84 AAGAGLVPAAASPRR177 pKa = 11.84 LAAAAGRR184 pKa = 11.84 CRR186 pKa = 11.84 RR187 pKa = 11.84 RR188 pKa = 11.84 RR189 pKa = 11.84 RR190 pKa = 11.84 GRR192 pKa = 11.84 RR193 pKa = 11.84 PGHH196 pKa = 4.57 RR197 pKa = 11.84 TARR200 pKa = 11.84 RR201 pKa = 11.84 RR202 pKa = 11.84 ALRR205 pKa = 11.84 AGQPGVAAGEE215 pKa = 3.95 PAGRR219 pKa = 11.84 ARR221 pKa = 11.84 RR222 pKa = 11.84 GAGHH226 pKa = 6.94 RR227 pKa = 11.84 AGAARR232 pKa = 11.84 HH233 pKa = 5.86 AGRR236 pKa = 11.84 AGGTPAGPGPGVAGRR251 pKa = 11.84 LAGALAGRR259 pKa = 11.84 GGAGRR264 pKa = 11.84 GRR266 pKa = 11.84 AARR269 pKa = 11.84 RR270 pKa = 11.84 GHH272 pKa = 6.35 RR273 pKa = 11.84 LAGRR277 pKa = 11.84 GPRR280 pKa = 11.84 RR281 pKa = 11.84 GAADD285 pKa = 3.59 RR286 pKa = 11.84 AGRR289 pKa = 11.84 GGRR292 pKa = 11.84 LAALAVPPRR301 pKa = 11.84 PAAGGGRR308 pKa = 11.84 PGRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 GARR316 pKa = 11.84 LAAAAGDD323 pKa = 4.0 AGLAAAGHH331 pKa = 6.08 RR332 pKa = 11.84 GGRR335 pKa = 11.84 LRR337 pKa = 11.84 RR338 pKa = 11.84 RR339 pKa = 11.84 AGGRR343 pKa = 11.84 AGPADD348 pKa = 3.76 GAGGGAAGGRR358 pKa = 11.84 RR359 pKa = 11.84 PGGRR363 pKa = 11.84 PGRR366 pKa = 11.84 PLPGPAGHH374 pKa = 6.64 RR375 pKa = 11.84 HH376 pKa = 5.21 AAAGAAHH383 pKa = 7.14 PPRR386 pKa = 11.84 RR387 pKa = 11.84 RR388 pKa = 11.84 SRR390 pKa = 11.84 RR391 pKa = 11.84 RR392 pKa = 11.84 PGRR395 pKa = 11.84 GAVRR399 pKa = 11.84 PGRR402 pKa = 11.84 RR403 pKa = 11.84 GGRR406 pKa = 11.84 HH407 pKa = 4.7 RR408 pKa = 11.84 HH409 pKa = 5.37 PLPGRR414 pKa = 11.84 GPARR418 pKa = 11.84 PAGGRR423 pKa = 11.84 GPPGRR428 pKa = 11.84 GAAGRR433 pKa = 11.84 ARR435 pKa = 11.84 AGGAPDD441 pKa = 3.5 RR442 pKa = 11.84 GQRR445 pKa = 11.84 DD446 pKa = 3.34 GRR448 pKa = 11.84 GARR451 pKa = 11.84 PGPGDD456 pKa = 3.23 RR457 pKa = 11.84 HH458 pKa = 5.35 GRR460 pKa = 11.84 AAGQRR465 pKa = 11.84 PVAGRR470 pKa = 11.84 PRR472 pKa = 11.84 HH473 pKa = 5.08 PARR476 pKa = 11.84 RR477 pKa = 11.84 PGAVDD482 pKa = 3.35 RR483 pKa = 4.97
Molecular weight: 48.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.413
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.12
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4421
0
4421
1365029
40
2842
308.8
32.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.864 ± 0.063
0.704 ± 0.01
6.169 ± 0.028
5.296 ± 0.031
2.399 ± 0.021
9.951 ± 0.032
2.052 ± 0.015
2.278 ± 0.025
1.099 ± 0.02
10.843 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.465 ± 0.014
1.277 ± 0.016
6.46 ± 0.031
2.638 ± 0.021
8.531 ± 0.039
4.51 ± 0.025
6.077 ± 0.029
10.247 ± 0.036
1.461 ± 0.016
1.678 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here