CRESS virus sp. ct6pe1
Average proteome isoelectric point is 8.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WBB5|A0A5Q2WBB5_9VIRU Rep protein OS=CRESS virus sp. ct6pe1 OX=2656676 PE=4 SV=1
MM1 pKa = 7.7 PAAQARR7 pKa = 11.84 RR8 pKa = 11.84 FCFTLNNYY16 pKa = 7.93 TPEE19 pKa = 4.29 DD20 pKa = 3.67 VAHH23 pKa = 6.9 IEE25 pKa = 4.17 SLYY28 pKa = 11.06 LNADD32 pKa = 3.01 NHH34 pKa = 5.06 ITYY37 pKa = 10.37 LVIGRR42 pKa = 11.84 EE43 pKa = 4.33 VGDD46 pKa = 3.43 SGTPHH51 pKa = 6.38 LQGFICFSRR60 pKa = 11.84 RR61 pKa = 11.84 KK62 pKa = 7.52 TFNVVKK68 pKa = 10.57 ALINEE73 pKa = 4.98 RR74 pKa = 11.84 IHH76 pKa = 8.47 IEE78 pKa = 4.06 GARR81 pKa = 11.84 GTSDD85 pKa = 3.86 QAATYY90 pKa = 9.19 CKK92 pKa = 10.12 KK93 pKa = 10.65 DD94 pKa = 3.08 GAYY97 pKa = 9.27 TEE99 pKa = 4.8 FGTVPVSGKK108 pKa = 8.36 TNYY111 pKa = 10.7 LEE113 pKa = 6.47 DD114 pKa = 3.86 FFKK117 pKa = 10.56 WADD120 pKa = 3.48 QFHH123 pKa = 7.33 EE124 pKa = 4.73 DD125 pKa = 3.63 NGRR128 pKa = 11.84 IPTARR133 pKa = 11.84 DD134 pKa = 2.93 VALEE138 pKa = 4.04 HH139 pKa = 6.43 PVVLTRR145 pKa = 11.84 HH146 pKa = 6.03 RR147 pKa = 11.84 NVLDD151 pKa = 3.54 VLEE154 pKa = 4.87 SRR156 pKa = 11.84 APPPSLVTNPQPRR169 pKa = 11.84 PWQLEE174 pKa = 4.29 LEE176 pKa = 4.28 EE177 pKa = 5.29 HH178 pKa = 6.16 LTDD181 pKa = 4.54 DD182 pKa = 4.05 TPDD185 pKa = 3.4 DD186 pKa = 3.59 RR187 pKa = 11.84 TVEE190 pKa = 4.05 FFVDD194 pKa = 3.56 EE195 pKa = 4.36 EE196 pKa = 4.52 GGKK199 pKa = 10.11 GKK201 pKa = 10.14 SWFQRR206 pKa = 11.84 HH207 pKa = 5.99 MLTKK211 pKa = 10.5 YY212 pKa = 9.53 PDD214 pKa = 3.87 RR215 pKa = 11.84 VQMLAPGKK223 pKa = 10.08 RR224 pKa = 11.84 DD225 pKa = 3.9 DD226 pKa = 4.31 LAHH229 pKa = 6.75 TIDD232 pKa = 3.36 IQKK235 pKa = 10.71 SIFLMNVPKK244 pKa = 9.55 TQMEE248 pKa = 4.36 FLQYY252 pKa = 10.87 SILEE256 pKa = 4.06 QLKK259 pKa = 10.27 DD260 pKa = 3.4 RR261 pKa = 11.84 TVFSPKK267 pKa = 8.73 YY268 pKa = 7.05 TSKK271 pKa = 10.38 MKK273 pKa = 10.61 VLSAPAHH280 pKa = 5.4 VIVFSNEE287 pKa = 3.72 HH288 pKa = 6.41 PDD290 pKa = 3.72 MNKK293 pKa = 9.18 MSADD297 pKa = 3.78 RR298 pKa = 11.84 YY299 pKa = 10.49 HH300 pKa = 6.34 ITILDD305 pKa = 3.51
Molecular weight: 34.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.89
IPC2_protein 5.753
IPC_protein 5.83
Toseland 6.338
ProMoST 6.097
Dawson 6.122
Bjellqvist 6.109
Wikipedia 6.148
Rodwell 6.109
Grimsley 6.532
Solomon 6.122
Lehninger 6.122
Nozaki 6.389
DTASelect 6.59
Thurlkill 6.605
EMBOSS 6.576
Sillero 6.504
Patrickios 4.304
IPC_peptide 6.135
IPC2_peptide 6.491
IPC2.peptide.svr19 6.437
Protein with the highest isoelectric point:
>tr|A0A5Q2WBB5|A0A5Q2WBB5_9VIRU Rep protein OS=CRESS virus sp. ct6pe1 OX=2656676 PE=4 SV=1
MM1 pKa = 7.0 KK2 pKa = 10.38 HH3 pKa = 5.13 NWTRR7 pKa = 11.84 PIVKK11 pKa = 10.0 FIEE14 pKa = 5.0 DD15 pKa = 4.05 DD16 pKa = 3.9 LVASGIGGATGPARR30 pKa = 11.84 FFAARR35 pKa = 11.84 GAGLVGDD42 pKa = 4.99 GVNKK46 pKa = 9.18 TWGSGDD52 pKa = 3.27 TRR54 pKa = 11.84 TWSAWDD60 pKa = 3.26 NMFVQKK66 pKa = 10.44 SGQYY70 pKa = 7.36 GQKK73 pKa = 9.64 RR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 FTPTRR81 pKa = 11.84 GKK83 pKa = 10.41 RR84 pKa = 11.84 GVRR87 pKa = 11.84 LYY89 pKa = 9.71 RR90 pKa = 11.84 RR91 pKa = 11.84 KK92 pKa = 10.18 YY93 pKa = 7.88 IRR95 pKa = 11.84 RR96 pKa = 11.84 RR97 pKa = 11.84 PRR99 pKa = 11.84 MMRR102 pKa = 11.84 RR103 pKa = 11.84 SRR105 pKa = 11.84 ASWGTRR111 pKa = 11.84 VRR113 pKa = 11.84 RR114 pKa = 11.84 AALRR118 pKa = 11.84 SNEE121 pKa = 4.35 SKK123 pKa = 10.22 RR124 pKa = 11.84 HH125 pKa = 4.09 QVTTITEE132 pKa = 4.73 AIDD135 pKa = 4.05 DD136 pKa = 4.07 QVLDD140 pKa = 3.75 TTAIARR146 pKa = 11.84 VPDD149 pKa = 4.18 LDD151 pKa = 3.57 QGAGGIEE158 pKa = 4.17 GMSSKK163 pKa = 10.3 ISRR166 pKa = 11.84 NGATIITTGIASWIHH181 pKa = 5.52 IRR183 pKa = 11.84 NSSTVQAMRR192 pKa = 11.84 VDD194 pKa = 4.28 LVWFYY199 pKa = 11.66 KK200 pKa = 10.49 KK201 pKa = 10.14 RR202 pKa = 11.84 DD203 pKa = 3.58 VGAVQAIFKK212 pKa = 10.21 DD213 pKa = 3.8 NQFEE217 pKa = 4.88 AINSPSDD224 pKa = 3.31 PTSYY228 pKa = 9.44 FARR231 pKa = 11.84 MIMPYY236 pKa = 10.88 GNLNTVILKK245 pKa = 10.58 KK246 pKa = 10.1 LTIDD250 pKa = 3.36 LAEE253 pKa = 4.71 AGEE256 pKa = 4.42 TAGTEE261 pKa = 3.96 SRR263 pKa = 11.84 MLKK266 pKa = 9.65 VWLPFKK272 pKa = 10.8 KK273 pKa = 10.45 VMKK276 pKa = 10.49 FNYY279 pKa = 8.95 TSEE282 pKa = 3.99 GTTNQDD288 pKa = 2.52 YY289 pKa = 10.73 DD290 pKa = 3.26 IHH292 pKa = 8.03 FGIRR296 pKa = 11.84 CHH298 pKa = 6.14 NKK300 pKa = 8.99 EE301 pKa = 3.79 GQAFSTTTNVLIAQHH316 pKa = 5.85 RR317 pKa = 11.84 HH318 pKa = 3.41 VLYY321 pKa = 10.79 FRR323 pKa = 11.84 DD324 pKa = 3.69 PP325 pKa = 3.42
Molecular weight: 36.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.736
IPC_protein 10.54
Toseland 10.76
ProMoST 10.452
Dawson 10.847
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 11.082
Grimsley 10.891
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.774
Patrickios 10.789
IPC_peptide 10.979
IPC2_peptide 9.355
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
691
61
325
230.3
26.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.946 ± 0.656
0.724 ± 0.263
6.078 ± 0.874
4.342 ± 1.029
4.631 ± 0.341
6.512 ± 1.3
3.039 ± 0.672
5.933 ± 0.576
5.355 ± 0.576
6.223 ± 1.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.039 ± 0.306
4.197 ± 0.141
4.631 ± 0.988
3.473 ± 0.524
8.828 ± 1.416
6.368 ± 1.291
8.104 ± 0.489
6.657 ± 0.341
1.592 ± 0.539
3.329 ± 0.527
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here