Streptococcus pyogenes serotype M1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus pyogenes

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9A0M1|Q9A0M1_STRP1 Putative DNase OS=Streptococcus pyogenes serotype M1 OX=301447 GN=mf2 PE=1 SV=1
MM1 pKa = 7.62ALAKK5 pKa = 9.78IVYY8 pKa = 10.47ASMTGNTEE16 pKa = 3.89EE17 pKa = 4.77IADD20 pKa = 3.55IVANKK25 pKa = 9.75LQEE28 pKa = 4.5LGHH31 pKa = 6.81DD32 pKa = 3.77VDD34 pKa = 4.83IDD36 pKa = 3.56EE37 pKa = 5.08CTTVDD42 pKa = 3.52ASEE45 pKa = 4.72FEE47 pKa = 4.22NADD50 pKa = 3.27IAVVATYY57 pKa = 9.21TYY59 pKa = 11.46GDD61 pKa = 3.61GDD63 pKa = 4.31LPDD66 pKa = 5.1EE67 pKa = 4.45IVDD70 pKa = 4.62FYY72 pKa = 11.83EE73 pKa = 5.1DD74 pKa = 4.17LQDD77 pKa = 5.1LDD79 pKa = 5.07LEE81 pKa = 4.47GKK83 pKa = 9.9IYY85 pKa = 10.62GVVGSGDD92 pKa = 3.05TFYY95 pKa = 11.14DD96 pKa = 3.99YY97 pKa = 11.07FCKK100 pKa = 10.66SVDD103 pKa = 3.68DD104 pKa = 4.51FSEE107 pKa = 4.2QFALTGAIKK116 pKa = 10.27GAEE119 pKa = 4.0PVKK122 pKa = 10.8VDD124 pKa = 5.02LAAEE128 pKa = 4.52DD129 pKa = 4.04EE130 pKa = 4.97DD131 pKa = 4.26IDD133 pKa = 4.05RR134 pKa = 11.84LEE136 pKa = 4.46AFAEE140 pKa = 4.11QLSQAVNNLL149 pKa = 3.23

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P66583|RS5_STRP1 30S ribosomal protein S5 OS=Streptococcus pyogenes serotype M1 OX=301447 GN=rpsE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.67IRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.52HH16 pKa = 5.2GFRR19 pKa = 11.84HH20 pKa = 6.39RR21 pKa = 11.84MSTKK25 pKa = 9.02NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1690

0

1690

515279

27

2045

304.9

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.822 ± 0.081

0.633 ± 0.017

5.779 ± 0.05

6.383 ± 0.061

4.403 ± 0.043

6.464 ± 0.065

2.056 ± 0.026

7.41 ± 0.062

7.034 ± 0.071

10.175 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.028

4.368 ± 0.034

3.353 ± 0.03

4.306 ± 0.044

3.965 ± 0.04

6.185 ± 0.058

5.876 ± 0.046

6.712 ± 0.051

0.843 ± 0.019

3.673 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski