Amino acid dipepetide frequency for Streptococcus pyogenes serotype M1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.113AlaAla: 6.113 ± 0.164
0.582AlaCys: 0.582 ± 0.037
4.376AlaAsp: 4.376 ± 0.1
4.627AlaGlu: 4.627 ± 0.108
3.662AlaPhe: 3.662 ± 0.101
5.125AlaGly: 5.125 ± 0.11
1.294AlaHis: 1.294 ± 0.053
6.777AlaIle: 6.777 ± 0.126
6.072AlaLys: 6.072 ± 0.15
7.953AlaLeu: 7.953 ± 0.147
2.092AlaMet: 2.092 ± 0.067
3.447AlaAsn: 3.447 ± 0.082
2.082AlaPro: 2.082 ± 0.072
3.344AlaGln: 3.344 ± 0.079
2.857AlaArg: 2.857 ± 0.081
4.743AlaSer: 4.743 ± 0.108
4.324AlaThr: 4.324 ± 0.091
5.147AlaVal: 5.147 ± 0.117
0.59AlaTrp: 0.59 ± 0.034
3.01AlaTyr: 3.01 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
0.33CysAla: 0.33 ± 0.024
0.08CysCys: 0.08 ± 0.015
0.392CysAsp: 0.392 ± 0.03
0.299CysGlu: 0.299 ± 0.023
0.406CysPhe: 0.406 ± 0.032
0.51CysGly: 0.51 ± 0.029
0.213CysHis: 0.213 ± 0.022
0.363CysIle: 0.363 ± 0.026
0.239CysLys: 0.239 ± 0.024
0.881CysLeu: 0.881 ± 0.047
0.132CysMet: 0.132 ± 0.016
0.223CysAsn: 0.223 ± 0.022
0.264CysPro: 0.264 ± 0.023
0.474CysGln: 0.474 ± 0.033
0.258CysArg: 0.258 ± 0.025
0.322CysSer: 0.322 ± 0.026
0.233CysThr: 0.233 ± 0.02
0.369CysVal: 0.369 ± 0.027
0.056CysTrp: 0.056 ± 0.009
0.283CysTyr: 0.283 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.914AspAla: 3.914 ± 0.109
0.392AspCys: 0.392 ± 0.029
3.158AspAsp: 3.158 ± 0.094
3.429AspGlu: 3.429 ± 0.089
2.987AspPhe: 2.987 ± 0.078
3.606AspGly: 3.606 ± 0.106
1.248AspHis: 1.248 ± 0.051
4.803AspIle: 4.803 ± 0.104
4.535AspLys: 4.535 ± 0.125
6.053AspLeu: 6.053 ± 0.107
1.609AspMet: 1.609 ± 0.048
2.61AspAsn: 2.61 ± 0.078
1.756AspPro: 1.756 ± 0.063
2.304AspGln: 2.304 ± 0.067
2.113AspArg: 2.113 ± 0.062
3.233AspSer: 3.233 ± 0.081
2.917AspThr: 2.917 ± 0.09
3.623AspVal: 3.623 ± 0.096
0.644AspTrp: 0.644 ± 0.041
2.857AspTyr: 2.857 ± 0.084
0.0AspXaa: 0.0 ± 0.0
Glu
5.576GluAla: 5.576 ± 0.136
0.312GluCys: 0.312 ± 0.028
3.557GluAsp: 3.557 ± 0.087
4.982GluGlu: 4.982 ± 0.138
2.154GluPhe: 2.154 ± 0.072
3.524GluGly: 3.524 ± 0.09
1.355GluHis: 1.355 ± 0.053
4.524GluIle: 4.524 ± 0.113
5.222GluLys: 5.222 ± 0.115
6.373GluLeu: 6.373 ± 0.124
1.739GluMet: 1.739 ± 0.067
3.198GluAsn: 3.198 ± 0.072
1.615GluPro: 1.615 ± 0.058
2.812GluGln: 2.812 ± 0.082
3.018GluArg: 3.018 ± 0.088
3.123GluSer: 3.123 ± 0.081
3.823GluThr: 3.823 ± 0.104
4.636GluVal: 4.636 ± 0.097
0.522GluTrp: 0.522 ± 0.034
1.768GluTyr: 1.768 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
3.28PheAla: 3.28 ± 0.082
0.307PheCys: 0.307 ± 0.027
3.033PheAsp: 3.033 ± 0.078
2.874PheGlu: 2.874 ± 0.082
1.995PhePhe: 1.995 ± 0.074
3.13PheGly: 3.13 ± 0.089
0.805PheHis: 0.805 ± 0.039
3.224PheIle: 3.224 ± 0.092
2.583PheLys: 2.583 ± 0.07
4.38PheLeu: 4.38 ± 0.124
1.149PheMet: 1.149 ± 0.048
1.935PheAsn: 1.935 ± 0.064
1.578PhePro: 1.578 ± 0.053
1.504PheGln: 1.504 ± 0.051
1.494PheArg: 1.494 ± 0.056
3.286PheSer: 3.286 ± 0.093
2.377PheThr: 2.377 ± 0.076
2.973PheVal: 2.973 ± 0.087
0.441PheTrp: 0.441 ± 0.032
1.686PheTyr: 1.686 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
4.658GlyAla: 4.658 ± 0.125
0.442GlyCys: 0.442 ± 0.03
3.507GlyAsp: 3.507 ± 0.09
3.408GlyGlu: 3.408 ± 0.088
3.227GlyPhe: 3.227 ± 0.087
4.235GlyGly: 4.235 ± 0.121
1.424GlyHis: 1.424 ± 0.055
5.432GlyIle: 5.432 ± 0.124
4.84GlyLys: 4.84 ± 0.113
6.761GlyLeu: 6.761 ± 0.131
1.733GlyMet: 1.733 ± 0.059
2.754GlyAsn: 2.754 ± 0.077
1.529GlyPro: 1.529 ± 0.064
3.037GlyGln: 3.037 ± 0.085
2.591GlyArg: 2.591 ± 0.082
3.598GlySer: 3.598 ± 0.094
3.621GlyThr: 3.621 ± 0.095
4.533GlyVal: 4.533 ± 0.115
0.629GlyTrp: 0.629 ± 0.044
2.678GlyTyr: 2.678 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
1.207HisAla: 1.207 ± 0.057
0.167HisCys: 0.167 ± 0.02
1.056HisAsp: 1.056 ± 0.043
1.025HisGlu: 1.025 ± 0.047
1.324HisPhe: 1.324 ± 0.047
1.223HisGly: 1.223 ± 0.05
0.695HisHis: 0.695 ± 0.039
1.556HisIle: 1.556 ± 0.055
1.106HisLys: 1.106 ± 0.046
2.482HisLeu: 2.482 ± 0.081
0.508HisMet: 0.508 ± 0.034
0.802HisAsn: 0.802 ± 0.038
0.92HisPro: 0.92 ± 0.04
1.176HisGln: 1.176 ± 0.047
0.827HisArg: 0.827 ± 0.042
1.1HisSer: 1.1 ± 0.047
0.924HisThr: 0.924 ± 0.041
1.182HisVal: 1.182 ± 0.05
0.173HisTrp: 0.173 ± 0.018
1.108HisTyr: 1.108 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.398IleAla: 6.398 ± 0.114
0.545IleCys: 0.545 ± 0.035
4.79IleAsp: 4.79 ± 0.1
4.815IleGlu: 4.815 ± 0.122
3.148IlePhe: 3.148 ± 0.096
5.069IleGly: 5.069 ± 0.116
1.287IleHis: 1.287 ± 0.056
5.372IleIle: 5.372 ± 0.131
5.081IleLys: 5.081 ± 0.109
6.87IleLeu: 6.87 ± 0.141
1.784IleMet: 1.784 ± 0.069
3.359IleAsn: 3.359 ± 0.079
2.878IlePro: 2.878 ± 0.075
2.467IleGln: 2.467 ± 0.07
2.863IleArg: 2.863 ± 0.086
5.147IleSer: 5.147 ± 0.119
4.398IleThr: 4.398 ± 0.106
4.867IleVal: 4.867 ± 0.107
0.543IleTrp: 0.543 ± 0.033
2.416IleTyr: 2.416 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
6.214LysAla: 6.214 ± 0.175
0.264LysCys: 0.264 ± 0.017
4.32LysAsp: 4.32 ± 0.116
6.127LysGlu: 6.127 ± 0.14
1.747LysPhe: 1.747 ± 0.066
4.328LysGly: 4.328 ± 0.103
1.444LysHis: 1.444 ± 0.051
4.339LysIle: 4.339 ± 0.113
5.574LysLys: 5.574 ± 0.133
5.785LysLeu: 5.785 ± 0.109
1.945LysMet: 1.945 ± 0.066
3.175LysAsn: 3.175 ± 0.083
2.304LysPro: 2.304 ± 0.074
3.214LysGln: 3.214 ± 0.082
3.396LysArg: 3.396 ± 0.1
4.006LysSer: 4.006 ± 0.095
4.617LysThr: 4.617 ± 0.101
4.776LysVal: 4.776 ± 0.106
0.625LysTrp: 0.625 ± 0.036
2.146LysTyr: 2.146 ± 0.067
0.0LysXaa: 0.0 ± 0.0
Leu
9.358LeuAla: 9.358 ± 0.161
0.607LeuCys: 0.607 ± 0.034
5.931LeuAsp: 5.931 ± 0.12
6.639LeuGlu: 6.639 ± 0.124
4.332LeuPhe: 4.332 ± 0.119
6.587LeuGly: 6.587 ± 0.144
1.584LeuHis: 1.584 ± 0.059
7.118LeuIle: 7.118 ± 0.149
6.825LeuLys: 6.825 ± 0.118
10.313LeuLeu: 10.313 ± 0.222
2.548LeuMet: 2.548 ± 0.078
4.176LeuAsn: 4.176 ± 0.095
3.907LeuPro: 3.907 ± 0.094
3.396LeuGln: 3.396 ± 0.098
3.612LeuArg: 3.612 ± 0.087
7.425LeuSer: 7.425 ± 0.147
6.812LeuThr: 6.812 ± 0.122
6.899LeuVal: 6.899 ± 0.121
0.704LeuTrp: 0.704 ± 0.038
2.981LeuTyr: 2.981 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
2.315MetAla: 2.315 ± 0.068
0.138MetCys: 0.138 ± 0.016
1.325MetAsp: 1.325 ± 0.056
1.36MetGlu: 1.36 ± 0.055
0.811MetPhe: 0.811 ± 0.046
1.675MetGly: 1.675 ± 0.069
0.332MetHis: 0.332 ± 0.023
2.117MetIle: 2.117 ± 0.071
1.71MetLys: 1.71 ± 0.063
2.364MetLeu: 2.364 ± 0.065
0.751MetMet: 0.751 ± 0.042
1.087MetAsn: 1.087 ± 0.046
0.943MetPro: 0.943 ± 0.044
0.935MetGln: 0.935 ± 0.046
1.019MetArg: 1.019 ± 0.042
1.83MetSer: 1.83 ± 0.06
2.42MetThr: 2.42 ± 0.066
1.776MetVal: 1.776 ± 0.057
0.14MetTrp: 0.14 ± 0.019
0.561MetTyr: 0.561 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.095AsnAla: 3.095 ± 0.08
0.295AsnCys: 0.295 ± 0.025
2.379AsnAsp: 2.379 ± 0.072
2.36AsnGlu: 2.36 ± 0.069
1.69AsnPhe: 1.69 ± 0.067
3.222AsnGly: 3.222 ± 0.088
1.271AsnHis: 1.271 ± 0.047
3.181AsnIle: 3.181 ± 0.085
2.886AsnLys: 2.886 ± 0.086
4.25AsnLeu: 4.25 ± 0.095
1.194AsnMet: 1.194 ± 0.048
1.906AsnAsn: 1.906 ± 0.074
2.129AsnPro: 2.129 ± 0.068
2.302AsnGln: 2.302 ± 0.076
2.049AsnArg: 2.049 ± 0.082
2.352AsnSer: 2.352 ± 0.083
2.228AsnThr: 2.228 ± 0.069
2.635AsnVal: 2.635 ± 0.075
0.446AsnTrp: 0.446 ± 0.03
1.805AsnTyr: 1.805 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
2.317ProAla: 2.317 ± 0.086
0.179ProCys: 0.179 ± 0.018
2.045ProAsp: 2.045 ± 0.062
2.637ProGlu: 2.637 ± 0.07
1.603ProPhe: 1.603 ± 0.06
1.78ProGly: 1.78 ± 0.068
0.687ProHis: 0.687 ± 0.039
2.616ProIle: 2.616 ± 0.074
2.54ProLys: 2.54 ± 0.079
3.103ProLeu: 3.103 ± 0.081
0.813ProMet: 0.813 ± 0.048
1.708ProAsn: 1.708 ± 0.06
0.567ProPro: 0.567 ± 0.036
1.419ProGln: 1.419 ± 0.055
1.009ProArg: 1.009 ± 0.04
2.232ProSer: 2.232 ± 0.075
2.034ProThr: 2.034 ± 0.055
2.54ProVal: 2.54 ± 0.079
0.274ProTrp: 0.274 ± 0.023
1.426ProTyr: 1.426 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
3.825GlnAla: 3.825 ± 0.1
0.184GlnCys: 0.184 ± 0.02
2.075GlnAsp: 2.075 ± 0.078
3.274GlnGlu: 3.274 ± 0.091
1.844GlnPhe: 1.844 ± 0.064
2.486GlnGly: 2.486 ± 0.085
0.809GlnHis: 0.809 ± 0.034
2.781GlnIle: 2.781 ± 0.072
3.392GlnLys: 3.392 ± 0.097
5.234GlnLeu: 5.234 ± 0.135
1.093GlnMet: 1.093 ± 0.055
1.679GlnAsn: 1.679 ± 0.062
1.302GlnPro: 1.302 ± 0.054
1.805GlnGln: 1.805 ± 0.068
1.562GlnArg: 1.562 ± 0.057
2.488GlnSer: 2.488 ± 0.071
2.451GlnThr: 2.451 ± 0.079
3.107GlnVal: 3.107 ± 0.086
0.363GlnTrp: 0.363 ± 0.029
1.302GlnTyr: 1.302 ± 0.052
0.0GlnXaa: 0.0 ± 0.0
Arg
2.498ArgAla: 2.498 ± 0.086
0.235ArgCys: 0.235 ± 0.024
2.278ArgAsp: 2.278 ± 0.067
2.614ArgGlu: 2.614 ± 0.082
1.902ArgPhe: 1.902 ± 0.064
2.346ArgGly: 2.346 ± 0.075
0.966ArgHis: 0.966 ± 0.04
2.721ArgIle: 2.721 ± 0.082
2.769ArgLys: 2.769 ± 0.084
4.386ArgLeu: 4.386 ± 0.112
1.075ArgMet: 1.075 ± 0.046
1.683ArgAsn: 1.683 ± 0.049
1.291ArgPro: 1.291 ± 0.058
2.358ArgGln: 2.358 ± 0.069
1.976ArgArg: 1.976 ± 0.076
2.022ArgSer: 2.022 ± 0.066
1.886ArgThr: 1.886 ± 0.06
2.705ArgVal: 2.705 ± 0.081
0.276ArgTrp: 0.276 ± 0.021
1.667ArgTyr: 1.667 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
3.998SerAla: 3.998 ± 0.092
0.363SerCys: 0.363 ± 0.029
3.532SerAsp: 3.532 ± 0.091
3.402SerGlu: 3.402 ± 0.086
3.119SerPhe: 3.119 ± 0.086
4.495SerGly: 4.495 ± 0.105
1.492SerHis: 1.492 ± 0.049
4.45SerIle: 4.45 ± 0.097
4.097SerLys: 4.097 ± 0.094
6.983SerLeu: 6.983 ± 0.128
1.471SerMet: 1.471 ± 0.055
2.521SerAsn: 2.521 ± 0.066
2.022SerPro: 2.022 ± 0.058
3.264SerGln: 3.264 ± 0.093
2.568SerArg: 2.568 ± 0.082
3.922SerSer: 3.922 ± 0.092
3.158SerThr: 3.158 ± 0.08
3.949SerVal: 3.949 ± 0.088
0.567SerTrp: 0.567 ± 0.029
2.474SerTyr: 2.474 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
4.392ThrAla: 4.392 ± 0.117
0.382ThrCys: 0.382 ± 0.028
3.305ThrAsp: 3.305 ± 0.084
3.256ThrGlu: 3.256 ± 0.097
2.831ThrPhe: 2.831 ± 0.093
4.103ThrGly: 4.103 ± 0.096
1.18ThrHis: 1.18 ± 0.044
4.819ThrIle: 4.819 ± 0.087
3.928ThrLys: 3.928 ± 0.097
5.853ThrLeu: 5.853 ± 0.114
1.263ThrMet: 1.263 ± 0.051
2.492ThrAsn: 2.492 ± 0.073
2.482ThrPro: 2.482 ± 0.075
2.183ThrGln: 2.183 ± 0.066
1.935ThrArg: 1.935 ± 0.058
3.829ThrSer: 3.829 ± 0.086
3.425ThrThr: 3.425 ± 0.102
4.258ThrVal: 4.258 ± 0.092
0.491ThrTrp: 0.491 ± 0.034
2.35ThrTyr: 2.35 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
5.671ValAla: 5.671 ± 0.126
0.505ValCys: 0.505 ± 0.036
3.936ValAsp: 3.936 ± 0.107
4.238ValGlu: 4.238 ± 0.116
2.983ValPhe: 2.983 ± 0.086
4.237ValGly: 4.237 ± 0.092
1.124ValHis: 1.124 ± 0.058
5.222ValIle: 5.222 ± 0.107
4.407ValLys: 4.407 ± 0.113
6.381ValLeu: 6.381 ± 0.129
1.778ValMet: 1.778 ± 0.057
3.018ValAsn: 3.018 ± 0.08
2.37ValPro: 2.37 ± 0.074
2.123ValGln: 2.123 ± 0.074
2.476ValArg: 2.476 ± 0.081
4.594ValSer: 4.594 ± 0.098
4.844ValThr: 4.844 ± 0.098
4.533ValVal: 4.533 ± 0.109
0.487ValTrp: 0.487 ± 0.033
2.191ValTyr: 2.191 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.549TrpAla: 0.549 ± 0.028
0.043TrpCys: 0.043 ± 0.009
0.477TrpAsp: 0.477 ± 0.031
0.466TrpGlu: 0.466 ± 0.03
0.417TrpPhe: 0.417 ± 0.03
0.569TrpGly: 0.569 ± 0.038
0.221TrpHis: 0.221 ± 0.021
0.547TrpIle: 0.547 ± 0.032
0.514TrpLys: 0.514 ± 0.032
0.998TrpLeu: 0.998 ± 0.057
0.229TrpMet: 0.229 ± 0.021
0.396TrpAsn: 0.396 ± 0.028
0.175TrpPro: 0.175 ± 0.02
0.518TrpGln: 0.518 ± 0.032
0.388TrpArg: 0.388 ± 0.03
0.545TrpSer: 0.545 ± 0.037
0.487TrpThr: 0.487 ± 0.032
0.474TrpVal: 0.474 ± 0.027
0.111TrpTrp: 0.111 ± 0.016
0.305TrpTyr: 0.305 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.509TyrAla: 2.509 ± 0.075
0.305TyrCys: 0.305 ± 0.024
2.319TyrAsp: 2.319 ± 0.072
2.001TyrGlu: 2.001 ± 0.066
1.853TyrPhe: 1.853 ± 0.059
2.391TyrGly: 2.391 ± 0.068
1.128TyrHis: 1.128 ± 0.049
2.133TyrIle: 2.133 ± 0.065
2.022TyrLys: 2.022 ± 0.074
4.351TyrLeu: 4.351 ± 0.113
0.703TyrMet: 0.703 ± 0.034
1.51TyrAsn: 1.51 ± 0.057
1.417TyrPro: 1.417 ± 0.055
2.422TyrGln: 2.422 ± 0.088
1.644TyrArg: 1.644 ± 0.062
2.049TyrSer: 2.049 ± 0.07
1.78TyrThr: 1.78 ± 0.066
2.133TyrVal: 2.133 ± 0.071
0.347TyrTrp: 0.347 ± 0.026
1.71TyrTyr: 1.71 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1690 proteins (515279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski