Mycoplasma meleagridis ATCC 25294

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis meleagridis

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5H077|A0A0F5H077_9MOLU Uncharacterized protein OS=Mycoplasma meleagridis ATCC 25294 OX=1264554 GN=MMELEA_01080 PE=4 SV=1
MM1 pKa = 7.52FSFLSSSTALSLEE14 pKa = 4.01LWLPPVVGFAAFSAFLISLIIEE36 pKa = 4.98DD37 pKa = 4.35ISASFCSYY45 pKa = 10.62SVYY48 pKa = 10.85EE49 pKa = 4.07LTLLVWKK56 pKa = 10.63LDD58 pKa = 4.1FILFNSANLLLPVSDD73 pKa = 3.41ITGSVYY79 pKa = 10.66VEE81 pKa = 4.39LVTKK85 pKa = 9.97VFVYY89 pKa = 10.18DD90 pKa = 2.95VWFFSASAIKK100 pKa = 10.42AFNWLFQSSVGDD112 pKa = 3.47VVLSVTISFFAFSNVSVALLLVFKK136 pKa = 10.98NPLSEE141 pKa = 4.03

Molecular weight: 15.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5H0Q4|A0A0F5H0Q4_9MOLU Uncharacterized protein OS=Mycoplasma meleagridis ATCC 25294 OX=1264554 GN=MMELEA_00920 PE=4 SV=1
MM1 pKa = 7.67SLRR4 pKa = 11.84TYY6 pKa = 10.45QPNKK10 pKa = 7.6RR11 pKa = 11.84QHH13 pKa = 5.54VKK15 pKa = 7.7THH17 pKa = 5.28GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTSNGRR29 pKa = 11.84KK30 pKa = 8.72ILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.81GRR40 pKa = 11.84KK41 pKa = 8.92RR42 pKa = 11.84LTVSSKK48 pKa = 10.99

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

505

0

505

193178

37

3604

382.5

43.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.219 ± 0.1

0.427 ± 0.026

5.464 ± 0.112

6.823 ± 0.106

5.274 ± 0.104

4.325 ± 0.115

1.255 ± 0.039

9.953 ± 0.154

10.552 ± 0.121

9.81 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.557 ± 0.047

9.52 ± 0.207

2.463 ± 0.065

2.924 ± 0.056

2.744 ± 0.076

6.633 ± 0.096

4.81 ± 0.069

5.169 ± 0.092

0.784 ± 0.036

4.295 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski