Desulfocarbo indianensis
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4217 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K9ICU1|A0A0K9ICU1_9DELT Uncharacterized protein OS=Desulfocarbo indianensis OX=1348163 GN=AAU61_06505 PE=4 SV=1
MM1 pKa = 7.69 GNPFCHH7 pKa = 7.64 LEE9 pKa = 4.07 LTTNSLPGAKK19 pKa = 9.56 AFYY22 pKa = 10.31 GALFDD27 pKa = 4.12 WQLSEE32 pKa = 4.45 YY33 pKa = 9.26 PSPDD37 pKa = 2.93 MPYY40 pKa = 9.96 TLINTGTEE48 pKa = 3.94 PGGGMMDD55 pKa = 4.04 LPDD58 pKa = 4.11 PQVPVGWTMYY68 pKa = 11.09 VLVDD72 pKa = 4.54 DD73 pKa = 5.1 VAAACAKK80 pKa = 10.14 ARR82 pKa = 11.84 EE83 pKa = 4.35 LGGQVFKK90 pKa = 11.06 GPQDD94 pKa = 3.27 IPGVGTFAIIGDD106 pKa = 3.71 PQGAVFGVWATLGDD120 pKa = 3.7
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.617
ProMoST 3.923
Dawson 3.834
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|A0A0K9IBU0|A0A0K9IBU0_9DELT C4-dicarboxylate ABC transporter permease OS=Desulfocarbo indianensis OX=1348163 GN=AAU61_04595 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNTKK11 pKa = 9.74 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 THH16 pKa = 5.75 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.53 GRR39 pKa = 11.84 QRR41 pKa = 11.84 LGVV44 pKa = 3.44
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4217
0
4217
1279174
31
1843
303.3
33.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.356 ± 0.051
1.239 ± 0.018
5.028 ± 0.029
6.401 ± 0.038
3.584 ± 0.028
8.653 ± 0.038
1.969 ± 0.017
4.457 ± 0.034
4.325 ± 0.035
11.466 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.021
2.664 ± 0.023
5.205 ± 0.034
3.925 ± 0.022
6.653 ± 0.038
5.119 ± 0.025
4.224 ± 0.03
7.175 ± 0.035
1.368 ± 0.019
2.588 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here