Alkalibacterium sp. 20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Alkalibacterium; unclassified Alkalibacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L8PA90|A0A1L8PA90_9LACT Fe-S cluster assembly ATPase SufC OS=Alkalibacterium sp. 20 OX=1798803 GN=AX762_02805 PE=3 SV=1
MM1 pKa = 7.53SKK3 pKa = 9.46MKK5 pKa = 10.56SVLKK9 pKa = 10.81YY10 pKa = 8.37GTIVFASSLFLSACGNGDD28 pKa = 4.06VPDD31 pKa = 4.57LDD33 pKa = 4.41EE34 pKa = 5.25DD35 pKa = 4.12ASSEE39 pKa = 4.05EE40 pKa = 4.25GAEE43 pKa = 4.21EE44 pKa = 4.13SAEE47 pKa = 4.07SLPEE51 pKa = 3.62VDD53 pKa = 5.51AGDD56 pKa = 4.24GSTLMVGLTNAPDD69 pKa = 3.66SFNPFYY75 pKa = 10.86RR76 pKa = 11.84PGVAGTWIQRR86 pKa = 11.84FFYY89 pKa = 10.69DD90 pKa = 3.73SLLVMPTADD99 pKa = 3.21SFEE102 pKa = 4.84PGLASFEE109 pKa = 4.46TEE111 pKa = 3.92DD112 pKa = 3.55NQVFTVNIDD121 pKa = 3.43PDD123 pKa = 4.46ANWSDD128 pKa = 4.19GEE130 pKa = 4.33PVSADD135 pKa = 2.97DD136 pKa = 5.14VAFTLNTIADD146 pKa = 4.08PQVEE150 pKa = 4.69TTLGTSVAMIEE161 pKa = 4.49GTDD164 pKa = 3.38SSGVRR169 pKa = 11.84EE170 pKa = 4.3EE171 pKa = 4.36GTEE174 pKa = 3.83EE175 pKa = 3.84LAGVEE180 pKa = 4.27VVDD183 pKa = 4.86EE184 pKa = 4.28KK185 pKa = 10.91TLTITTKK192 pKa = 10.8SPVDD196 pKa = 3.45LAYY199 pKa = 10.79LSEE202 pKa = 3.99FLGFNVLIAPKK213 pKa = 10.17HH214 pKa = 4.8VFEE217 pKa = 6.34DD218 pKa = 3.73IEE220 pKa = 4.45IVDD223 pKa = 3.84IPNSQEE229 pKa = 3.28ATMPSVFSGAYY240 pKa = 9.31QFVEE244 pKa = 4.27YY245 pKa = 11.07DD246 pKa = 3.0NDD248 pKa = 3.85NYY250 pKa = 10.86VHH252 pKa = 7.05LEE254 pKa = 3.8ANPDD258 pKa = 3.69YY259 pKa = 10.89FRR261 pKa = 11.84GAPAIEE267 pKa = 3.91TVYY270 pKa = 10.92ARR272 pKa = 11.84VMNGTSLITEE282 pKa = 4.49FQAGNLHH289 pKa = 5.38MTAGGGIGMVPVQDD303 pKa = 3.42IGLLEE308 pKa = 5.15DD309 pKa = 3.42IEE311 pKa = 4.6GLVVEE316 pKa = 5.03EE317 pKa = 4.64HH318 pKa = 7.04PSFNGQYY325 pKa = 9.62MVINNEE331 pKa = 3.79KK332 pKa = 10.16FDD334 pKa = 4.0NLEE337 pKa = 3.61VRR339 pKa = 11.84QAFAYY344 pKa = 10.12ALNRR348 pKa = 11.84EE349 pKa = 4.32LTVEE353 pKa = 3.95NLLGGRR359 pKa = 11.84GEE361 pKa = 4.44VLASTYY367 pKa = 11.11SSASPYY373 pKa = 10.39KK374 pKa = 10.32DD375 pKa = 4.47DD376 pKa = 3.61SLEE379 pKa = 4.02PLEE382 pKa = 4.76YY383 pKa = 10.66DD384 pKa = 3.4PEE386 pKa = 4.3LAKK389 pKa = 10.93EE390 pKa = 4.05MLEE393 pKa = 4.02EE394 pKa = 4.94AGFDD398 pKa = 3.4FDD400 pKa = 4.11TPLEE404 pKa = 4.24FVVPTGNAVRR414 pKa = 11.84EE415 pKa = 4.2QNGNLIEE422 pKa = 4.1QWFSEE427 pKa = 3.8IGVTVNLNSYY437 pKa = 10.2DD438 pKa = 3.69FPTWLSMAQDD448 pKa = 3.96LDD450 pKa = 3.9YY451 pKa = 11.21DD452 pKa = 3.74IGLMGWGHH460 pKa = 5.48TVDD463 pKa = 4.61PNIASYY469 pKa = 10.3IQTGGASNNMAVSDD483 pKa = 4.39PVVDD487 pKa = 4.96DD488 pKa = 5.7LIEE491 pKa = 4.02QGMAGTDD498 pKa = 3.49FDD500 pKa = 4.04EE501 pKa = 5.48RR502 pKa = 11.84YY503 pKa = 9.52PIYY506 pKa = 10.98AEE508 pKa = 4.44LQQHH512 pKa = 5.74MQDD515 pKa = 3.48EE516 pKa = 4.6MQIVPLYY523 pKa = 10.45SDD525 pKa = 3.29SQFSVQVDD533 pKa = 3.6NLNGGIKK540 pKa = 9.76EE541 pKa = 3.97YY542 pKa = 9.94WAGSLHH548 pKa = 7.38DD549 pKa = 3.87LHH551 pKa = 7.01EE552 pKa = 4.38WTLDD556 pKa = 3.47TEE558 pKa = 4.57EE559 pKa = 4.16

Molecular weight: 61.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L8PPM8|A0A1L8PPM8_9LACT Formate acetyltransferase OS=Alkalibacterium sp. 20 OX=1798803 GN=AX762_00280 PE=3 SV=1
MM1 pKa = 7.3AQGLTHH7 pKa = 5.92QPKK10 pKa = 8.82KK11 pKa = 10.23RR12 pKa = 11.84KK13 pKa = 7.68RR14 pKa = 11.84QRR16 pKa = 11.84VHH18 pKa = 6.34GFRR21 pKa = 11.84KK22 pKa = 10.03RR23 pKa = 11.84MSTKK27 pKa = 9.73SGRR30 pKa = 11.84NILRR34 pKa = 11.84NRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 8.93GRR41 pKa = 11.84KK42 pKa = 9.14NISAA46 pKa = 4.08

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2261

0

2261

687154

37

2011

303.9

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.66 ± 0.051

0.481 ± 0.011

5.889 ± 0.049

8.077 ± 0.072

4.449 ± 0.043

6.517 ± 0.048

1.937 ± 0.021

7.773 ± 0.05

6.864 ± 0.058

9.676 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.022

4.697 ± 0.034

3.321 ± 0.029

3.472 ± 0.032

3.934 ± 0.032

6.261 ± 0.035

5.749 ± 0.029

6.927 ± 0.039

0.905 ± 0.019

3.668 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski