Amino acid dipepetide frequency for Alkalibacterium sp. 20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.772AlaAla: 4.772 ± 0.123
0.423AlaCys: 0.423 ± 0.022
3.619AlaAsp: 3.619 ± 0.085
4.928AlaGlu: 4.928 ± 0.111
3.203AlaPhe: 3.203 ± 0.077
4.654AlaGly: 4.654 ± 0.096
1.198AlaHis: 1.198 ± 0.04
5.849AlaIle: 5.849 ± 0.111
4.174AlaLys: 4.174 ± 0.093
7.228AlaLeu: 7.228 ± 0.102
1.88AlaMet: 1.88 ± 0.054
2.669AlaAsn: 2.669 ± 0.067
1.733AlaPro: 1.733 ± 0.047
2.176AlaGln: 2.176 ± 0.056
2.57AlaArg: 2.57 ± 0.069
4.046AlaSer: 4.046 ± 0.085
3.654AlaThr: 3.654 ± 0.064
4.916AlaVal: 4.916 ± 0.092
0.506AlaTrp: 0.506 ± 0.03
2.406AlaTyr: 2.406 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.285CysAla: 0.285 ± 0.021
0.051CysCys: 0.051 ± 0.011
0.31CysAsp: 0.31 ± 0.021
0.323CysGlu: 0.323 ± 0.021
0.22CysPhe: 0.22 ± 0.02
0.466CysGly: 0.466 ± 0.027
0.134CysHis: 0.134 ± 0.014
0.291CysIle: 0.291 ± 0.021
0.223CysLys: 0.223 ± 0.019
0.444CysLeu: 0.444 ± 0.026
0.083CysMet: 0.083 ± 0.01
0.188CysAsn: 0.188 ± 0.017
0.244CysPro: 0.244 ± 0.022
0.172CysGln: 0.172 ± 0.015
0.221CysArg: 0.221 ± 0.017
0.351CysSer: 0.351 ± 0.021
0.256CysThr: 0.256 ± 0.018
0.338CysVal: 0.338 ± 0.023
0.045CysTrp: 0.045 ± 0.007
0.17CysTyr: 0.17 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.701AspAla: 3.701 ± 0.086
0.298AspCys: 0.298 ± 0.022
3.285AspAsp: 3.285 ± 0.08
5.214AspGlu: 5.214 ± 0.111
2.678AspPhe: 2.678 ± 0.06
3.57AspGly: 3.57 ± 0.088
1.17AspHis: 1.17 ± 0.04
4.671AspIle: 4.671 ± 0.084
3.944AspLys: 3.944 ± 0.073
5.549AspLeu: 5.549 ± 0.088
1.663AspMet: 1.663 ± 0.056
2.5AspAsn: 2.5 ± 0.06
2.107AspPro: 2.107 ± 0.065
1.959AspGln: 1.959 ± 0.055
2.33AspArg: 2.33 ± 0.062
3.394AspSer: 3.394 ± 0.061
3.142AspThr: 3.142 ± 0.069
4.274AspVal: 4.274 ± 0.086
0.649AspTrp: 0.649 ± 0.03
2.788AspTyr: 2.788 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.834GluAla: 5.834 ± 0.108
0.306GluCys: 0.306 ± 0.022
4.802GluAsp: 4.802 ± 0.111
8.139GluGlu: 8.139 ± 0.156
2.561GluPhe: 2.561 ± 0.066
4.488GluGly: 4.488 ± 0.088
1.371GluHis: 1.371 ± 0.046
5.75GluIle: 5.75 ± 0.097
6.806GluLys: 6.806 ± 0.134
7.425GluLeu: 7.425 ± 0.121
2.553GluMet: 2.553 ± 0.069
4.194GluAsn: 4.194 ± 0.07
2.176GluPro: 2.176 ± 0.081
3.056GluGln: 3.056 ± 0.067
3.481GluArg: 3.481 ± 0.073
4.353GluSer: 4.353 ± 0.086
4.951GluThr: 4.951 ± 0.091
5.226GluVal: 5.226 ± 0.109
0.875GluTrp: 0.875 ± 0.039
2.419GluTyr: 2.419 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
2.652PheAla: 2.652 ± 0.069
0.188PheCys: 0.188 ± 0.014
2.855PheAsp: 2.855 ± 0.062
3.058PheGlu: 3.058 ± 0.063
2.256PhePhe: 2.256 ± 0.067
3.26PheGly: 3.26 ± 0.078
0.768PheHis: 0.768 ± 0.03
3.647PheIle: 3.647 ± 0.084
2.649PheLys: 2.649 ± 0.072
4.213PheLeu: 4.213 ± 0.086
1.112PheMet: 1.112 ± 0.048
2.179PheAsn: 2.179 ± 0.053
1.486PhePro: 1.486 ± 0.043
1.428PheGln: 1.428 ± 0.047
1.47PheArg: 1.47 ± 0.045
3.515PheSer: 3.515 ± 0.076
2.457PheThr: 2.457 ± 0.064
3.113PheVal: 3.113 ± 0.077
0.416PheTrp: 0.416 ± 0.024
1.775PheTyr: 1.775 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.35GlyAla: 4.35 ± 0.094
0.394GlyCys: 0.394 ± 0.024
3.248GlyAsp: 3.248 ± 0.074
4.644GlyGlu: 4.644 ± 0.122
3.283GlyPhe: 3.283 ± 0.079
4.354GlyGly: 4.354 ± 0.092
1.353GlyHis: 1.353 ± 0.048
5.661GlyIle: 5.661 ± 0.091
4.471GlyLys: 4.471 ± 0.087
6.534GlyLeu: 6.534 ± 0.116
1.95GlyMet: 1.95 ± 0.069
2.66GlyAsn: 2.66 ± 0.067
1.608GlyPro: 1.608 ± 0.052
2.119GlyGln: 2.119 ± 0.054
2.468GlyArg: 2.468 ± 0.065
3.801GlySer: 3.801 ± 0.086
4.006GlyThr: 4.006 ± 0.084
4.737GlyVal: 4.737 ± 0.089
0.697GlyTrp: 0.697 ± 0.036
2.835GlyTyr: 2.835 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.253HisAla: 1.253 ± 0.046
0.102HisCys: 0.102 ± 0.011
1.07HisAsp: 1.07 ± 0.037
1.243HisGlu: 1.243 ± 0.047
1.016HisPhe: 1.016 ± 0.039
1.268HisGly: 1.268 ± 0.042
0.562HisHis: 0.562 ± 0.027
1.316HisIle: 1.316 ± 0.046
1.029HisLys: 1.029 ± 0.039
2.036HisLeu: 2.036 ± 0.061
0.52HisMet: 0.52 ± 0.029
0.728HisAsn: 0.728 ± 0.031
1.042HisPro: 1.042 ± 0.041
0.754HisGln: 0.754 ± 0.03
0.758HisArg: 0.758 ± 0.035
1.224HisSer: 1.224 ± 0.041
1.091HisThr: 1.091 ± 0.041
1.294HisVal: 1.294 ± 0.043
0.148HisTrp: 0.148 ± 0.014
0.921HisTyr: 0.921 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.772IleAla: 5.772 ± 0.108
0.4IleCys: 0.4 ± 0.025
5.04IleAsp: 5.04 ± 0.086
6.453IleGlu: 6.453 ± 0.093
3.13IlePhe: 3.13 ± 0.087
5.665IleGly: 5.665 ± 0.111
1.47IleHis: 1.47 ± 0.048
6.101IleIle: 6.101 ± 0.121
4.964IleLys: 4.964 ± 0.106
6.978IleLeu: 6.978 ± 0.128
1.775IleMet: 1.775 ± 0.047
3.576IleAsn: 3.576 ± 0.077
3.055IlePro: 3.055 ± 0.073
2.574IleGln: 2.574 ± 0.058
2.966IleArg: 2.966 ± 0.075
4.875IleSer: 4.875 ± 0.087
4.306IleThr: 4.306 ± 0.089
5.568IleVal: 5.568 ± 0.1
0.563IleTrp: 0.563 ± 0.031
2.5IleTyr: 2.5 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
4.692LysAla: 4.692 ± 0.093
0.253LysCys: 0.253 ± 0.02
4.244LysAsp: 4.244 ± 0.083
7.445LysGlu: 7.445 ± 0.114
1.771LysPhe: 1.771 ± 0.055
4.073LysGly: 4.073 ± 0.082
1.288LysHis: 1.288 ± 0.043
4.651LysIle: 4.651 ± 0.091
6.249LysLys: 6.249 ± 0.108
5.414LysLeu: 5.414 ± 0.096
2.052LysMet: 2.052 ± 0.062
3.692LysAsn: 3.692 ± 0.081
1.957LysPro: 1.957 ± 0.065
2.877LysGln: 2.877 ± 0.068
3.317LysArg: 3.317 ± 0.075
3.645LysSer: 3.645 ± 0.073
3.749LysThr: 3.749 ± 0.083
4.412LysVal: 4.412 ± 0.078
0.72LysTrp: 0.72 ± 0.032
2.139LysTyr: 2.139 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
6.622LeuAla: 6.622 ± 0.113
0.435LeuCys: 0.435 ± 0.028
5.639LeuAsp: 5.639 ± 0.097
6.996LeuGlu: 6.996 ± 0.094
4.816LeuPhe: 4.816 ± 0.104
6.025LeuGly: 6.025 ± 0.109
1.669LeuHis: 1.669 ± 0.045
7.565LeuIle: 7.565 ± 0.136
6.638LeuLys: 6.638 ± 0.103
9.552LeuLeu: 9.552 ± 0.148
2.611LeuMet: 2.611 ± 0.071
5.153LeuAsn: 5.153 ± 0.083
3.529LeuPro: 3.529 ± 0.075
2.638LeuGln: 2.638 ± 0.062
3.373LeuArg: 3.373 ± 0.07
7.367LeuSer: 7.367 ± 0.104
6.025LeuThr: 6.025 ± 0.102
6.105LeuVal: 6.105 ± 0.096
0.704LeuTrp: 0.704 ± 0.035
3.295LeuTyr: 3.295 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
1.899MetAla: 1.899 ± 0.056
0.112MetCys: 0.112 ± 0.013
1.719MetAsp: 1.719 ± 0.046
1.937MetGlu: 1.937 ± 0.051
0.969MetPhe: 0.969 ± 0.042
1.818MetGly: 1.818 ± 0.054
0.517MetHis: 0.517 ± 0.03
2.225MetIle: 2.225 ± 0.067
2.215MetLys: 2.215 ± 0.056
2.289MetLeu: 2.289 ± 0.057
0.809MetMet: 0.809 ± 0.039
1.61MetAsn: 1.61 ± 0.048
0.969MetPro: 0.969 ± 0.037
0.959MetGln: 0.959 ± 0.034
1.182MetArg: 1.182 ± 0.044
1.841MetSer: 1.841 ± 0.055
1.707MetThr: 1.707 ± 0.046
1.735MetVal: 1.735 ± 0.049
0.17MetTrp: 0.17 ± 0.016
0.747MetTyr: 0.747 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.804AsnAla: 2.804 ± 0.079
0.226AsnCys: 0.226 ± 0.018
2.815AsnAsp: 2.815 ± 0.065
4.057AsnGlu: 4.057 ± 0.078
1.761AsnPhe: 1.761 ± 0.045
3.117AsnGly: 3.117 ± 0.072
1.097AsnHis: 1.097 ± 0.043
3.519AsnIle: 3.519 ± 0.073
3.459AsnLys: 3.459 ± 0.093
3.967AsnLeu: 3.967 ± 0.083
1.24AsnMet: 1.24 ± 0.038
2.347AsnAsn: 2.347 ± 0.063
2.048AsnPro: 2.048 ± 0.049
2.229AsnGln: 2.229 ± 0.058
2.128AsnArg: 2.128 ± 0.057
2.28AsnSer: 2.28 ± 0.057
2.438AsnThr: 2.438 ± 0.068
3.062AsnVal: 3.062 ± 0.067
0.549AsnTrp: 0.549 ± 0.026
1.826AsnTyr: 1.826 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
2.027ProAla: 2.027 ± 0.049
0.153ProCys: 0.153 ± 0.015
2.213ProAsp: 2.213 ± 0.056
3.123ProGlu: 3.123 ± 0.074
1.775ProPhe: 1.775 ± 0.043
2.021ProGly: 2.021 ± 0.075
0.671ProHis: 0.671 ± 0.032
2.547ProIle: 2.547 ± 0.061
2.093ProLys: 2.093 ± 0.054
3.263ProLeu: 3.263 ± 0.075
0.796ProMet: 0.796 ± 0.035
1.55ProAsn: 1.55 ± 0.046
0.621ProPro: 0.621 ± 0.031
1.01ProGln: 1.01 ± 0.039
1.036ProArg: 1.036 ± 0.039
2.091ProSer: 2.091 ± 0.054
1.92ProThr: 1.92 ± 0.056
2.598ProVal: 2.598 ± 0.067
0.3ProTrp: 0.3 ± 0.021
1.401ProTyr: 1.401 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.685GlnAla: 2.685 ± 0.07
0.125GlnCys: 0.125 ± 0.015
1.741GlnAsp: 1.741 ± 0.046
2.637GlnGlu: 2.637 ± 0.064
1.496GlnPhe: 1.496 ± 0.054
1.968GlnGly: 1.968 ± 0.055
0.667GlnHis: 0.667 ± 0.032
2.356GlnIle: 2.356 ± 0.065
2.427GlnLys: 2.427 ± 0.063
3.951GlnLeu: 3.951 ± 0.079
1.089GlnMet: 1.089 ± 0.04
1.563GlnAsn: 1.563 ± 0.047
1.112GlnPro: 1.112 ± 0.04
1.346GlnGln: 1.346 ± 0.051
1.364GlnArg: 1.364 ± 0.051
2.157GlnSer: 2.157 ± 0.062
2.278GlnThr: 2.278 ± 0.07
2.245GlnVal: 2.245 ± 0.059
0.357GlnTrp: 0.357 ± 0.022
1.157GlnTyr: 1.157 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.392ArgAla: 2.392 ± 0.059
0.176ArgCys: 0.176 ± 0.017
2.165ArgAsp: 2.165 ± 0.055
3.334ArgGlu: 3.334 ± 0.077
1.882ArgPhe: 1.882 ± 0.058
2.179ArgGly: 2.179 ± 0.059
0.751ArgHis: 0.751 ± 0.036
3.023ArgIle: 3.023 ± 0.071
2.98ArgLys: 2.98 ± 0.07
4.009ArgLeu: 4.009 ± 0.083
1.278ArgMet: 1.278 ± 0.048
1.789ArgAsn: 1.789 ± 0.05
1.238ArgPro: 1.238 ± 0.039
1.544ArgGln: 1.544 ± 0.051
1.746ArgArg: 1.746 ± 0.057
2.213ArgSer: 2.213 ± 0.066
2.045ArgThr: 2.045 ± 0.05
2.666ArgVal: 2.666 ± 0.067
0.327ArgTrp: 0.327 ± 0.024
1.598ArgTyr: 1.598 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
3.701SerAla: 3.701 ± 0.079
0.274SerCys: 0.274 ± 0.023
3.701SerAsp: 3.701 ± 0.08
4.669SerGlu: 4.669 ± 0.09
3.314SerPhe: 3.314 ± 0.073
4.532SerGly: 4.532 ± 0.074
1.244SerHis: 1.244 ± 0.043
5.12SerIle: 5.12 ± 0.108
3.832SerLys: 3.832 ± 0.071
6.512SerLeu: 6.512 ± 0.105
1.595SerMet: 1.595 ± 0.048
2.752SerAsn: 2.752 ± 0.067
1.944SerPro: 1.944 ± 0.055
2.248SerGln: 2.248 ± 0.06
2.443SerArg: 2.443 ± 0.059
4.021SerSer: 4.021 ± 0.092
3.305SerThr: 3.305 ± 0.073
4.565SerVal: 4.565 ± 0.076
0.531SerTrp: 0.531 ± 0.029
2.307SerTyr: 2.307 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
3.724ThrAla: 3.724 ± 0.085
0.252ThrCys: 0.252 ± 0.019
3.531ThrAsp: 3.531 ± 0.076
4.196ThrGlu: 4.196 ± 0.096
2.769ThrPhe: 2.769 ± 0.063
4.219ThrGly: 4.219 ± 0.084
1.225ThrHis: 1.225 ± 0.044
4.655ThrIle: 4.655 ± 0.086
3.302ThrLys: 3.302 ± 0.061
5.884ThrLeu: 5.884 ± 0.097
1.343ThrMet: 1.343 ± 0.047
2.542ThrAsn: 2.542 ± 0.067
2.141ThrPro: 2.141 ± 0.062
1.835ThrGln: 1.835 ± 0.064
2.024ThrArg: 2.024 ± 0.052
3.442ThrSer: 3.442 ± 0.074
3.187ThrThr: 3.187 ± 0.074
4.6ThrVal: 4.6 ± 0.086
0.476ThrTrp: 0.476 ± 0.029
2.141ThrTyr: 2.141 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.667ValAla: 4.667 ± 0.09
0.378ValCys: 0.378 ± 0.026
4.07ValAsp: 4.07 ± 0.088
5.272ValGlu: 5.272 ± 0.095
3.301ValPhe: 3.301 ± 0.072
4.545ValGly: 4.545 ± 0.092
1.215ValHis: 1.215 ± 0.039
5.438ValIle: 5.438 ± 0.095
4.369ValLys: 4.369 ± 0.068
6.87ValLeu: 6.87 ± 0.102
1.952ValMet: 1.952 ± 0.054
3.149ValAsn: 3.149 ± 0.066
2.468ValPro: 2.468 ± 0.06
1.978ValGln: 1.978 ± 0.053
2.486ValArg: 2.486 ± 0.063
4.901ValSer: 4.901 ± 0.092
4.292ValThr: 4.292 ± 0.074
4.923ValVal: 4.923 ± 0.09
0.578ValTrp: 0.578 ± 0.032
2.416ValTyr: 2.416 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.46TrpAla: 0.46 ± 0.029
0.047TrpCys: 0.047 ± 0.008
0.537TrpAsp: 0.537 ± 0.032
0.605TrpGlu: 0.605 ± 0.033
0.457TrpPhe: 0.457 ± 0.025
0.518TrpGly: 0.518 ± 0.029
0.188TrpHis: 0.188 ± 0.016
0.739TrpIle: 0.739 ± 0.038
0.604TrpLys: 0.604 ± 0.032
0.985TrpLeu: 0.985 ± 0.046
0.313TrpMet: 0.313 ± 0.022
0.49TrpAsn: 0.49 ± 0.03
0.237TrpPro: 0.237 ± 0.019
0.359TrpGln: 0.359 ± 0.026
0.371TrpArg: 0.371 ± 0.028
0.578TrpSer: 0.578 ± 0.029
0.528TrpThr: 0.528 ± 0.025
0.61TrpVal: 0.61 ± 0.032
0.108TrpTrp: 0.108 ± 0.013
0.317TrpTyr: 0.317 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.312TyrAla: 2.312 ± 0.061
0.221TyrCys: 0.221 ± 0.018
2.283TyrAsp: 2.283 ± 0.065
2.493TyrGlu: 2.493 ± 0.065
1.837TyrPhe: 1.837 ± 0.062
2.436TyrGly: 2.436 ± 0.068
0.815TyrHis: 0.815 ± 0.035
2.608TyrIle: 2.608 ± 0.062
2.215TyrLys: 2.215 ± 0.059
3.651TyrLeu: 3.651 ± 0.076
0.814TyrMet: 0.814 ± 0.033
1.639TyrAsn: 1.639 ± 0.049
1.493TyrPro: 1.493 ± 0.054
1.458TyrGln: 1.458 ± 0.052
1.602TyrArg: 1.602 ± 0.056
2.515TyrSer: 2.515 ± 0.066
2.152TyrThr: 2.152 ± 0.05
2.282TyrVal: 2.282 ± 0.059
0.332TyrTrp: 0.332 ± 0.023
1.525TyrTyr: 1.525 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2261 proteins (687154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski