Aeoliella mucimassa

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales;

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518AQP7|A0A518AQP7_9BACT Uncharacterized protein OS=Aeoliella mucimassa OX=2527972 GN=Pan181_32620 PE=4 SV=1
MM1 pKa = 7.69HH2 pKa = 7.21NRR4 pKa = 11.84PLYY7 pKa = 9.37WALLLLSATALNHH20 pKa = 4.61VWLRR24 pKa = 11.84SCTAAPAAFWFSTDD38 pKa = 2.7ATLPGTPGVPEE49 pKa = 3.92FTSAVGIPRR58 pKa = 11.84TLHH61 pKa = 5.84IWAQPNTLTSGDD73 pKa = 3.65WNASTNPFLKK83 pKa = 10.21FQNVSLDD90 pKa = 3.3IVSPEE95 pKa = 3.88STFDD99 pKa = 3.41IDD101 pKa = 3.39PDD103 pKa = 4.27SIVVYY108 pKa = 10.49NPTYY112 pKa = 11.0ASGEE116 pKa = 4.03DD117 pKa = 3.25RR118 pKa = 11.84FSNVGDD124 pKa = 3.64SSTGLTEE131 pKa = 4.23SDD133 pKa = 3.36GSALGSYY140 pKa = 10.83DD141 pKa = 4.03DD142 pKa = 5.16LPIGFSKK149 pKa = 10.94GLLDD153 pKa = 3.75LQGFSMPANYY163 pKa = 10.25AQGFGVTCDD172 pKa = 4.33ASDD175 pKa = 4.17SYY177 pKa = 11.73CGTTDD182 pKa = 2.91SGSPAWLFASFAVTPTSDD200 pKa = 3.11TGSVEE205 pKa = 3.74FWMQIGRR212 pKa = 11.84NGINGVDD219 pKa = 3.82ASDD222 pKa = 3.96ALLGSGTIEE231 pKa = 3.91VQFGIDD237 pKa = 3.42TEE239 pKa = 4.71GFAPFDD245 pKa = 3.65YY246 pKa = 10.77DD247 pKa = 3.94AEE249 pKa = 4.3YY250 pKa = 11.04DD251 pKa = 4.09RR252 pKa = 11.84KK253 pKa = 9.59LTLANDD259 pKa = 4.07DD260 pKa = 3.91SDD262 pKa = 4.17VTLLLAGPGDD272 pKa = 4.16YY273 pKa = 10.94NADD276 pKa = 3.69GAVDD280 pKa = 3.76EE281 pKa = 5.31LDD283 pKa = 3.54YY284 pKa = 11.5AVWKK288 pKa = 10.55KK289 pKa = 11.02DD290 pKa = 3.28FGTNSYY296 pKa = 10.86DD297 pKa = 3.83ADD299 pKa = 4.06GNGDD303 pKa = 4.28GIVSLADD310 pKa = 3.23YY311 pKa = 7.58TIWRR315 pKa = 11.84DD316 pKa = 3.59NLDD319 pKa = 3.63ATSTGALASLNVSQVPEE336 pKa = 4.07PSTFAMLAGFTTLAWNLSSSSRR358 pKa = 11.84ARR360 pKa = 11.84HH361 pKa = 5.64SSNCEE366 pKa = 3.4

Molecular weight: 38.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518AKX6|A0A518AKX6_9BACT Dihydroorotase OS=Aeoliella mucimassa OX=2527972 GN=pyrC PE=3 SV=1
MM1 pKa = 7.44MKK3 pKa = 10.19RR4 pKa = 11.84AFDD7 pKa = 3.44IVGALVLLAVLSPLFLLVAVWMKK30 pKa = 10.57YY31 pKa = 6.85EE32 pKa = 3.87SRR34 pKa = 11.84GPVLCRR40 pKa = 11.84EE41 pKa = 4.0RR42 pKa = 11.84RR43 pKa = 11.84AGRR46 pKa = 11.84GGLPFRR52 pKa = 11.84TFKK55 pKa = 10.77FRR57 pKa = 11.84VLHH60 pKa = 6.18ASSAALCGTSTPRR73 pKa = 11.84NDD75 pKa = 3.8PQLSASGRR83 pKa = 11.84LMRR86 pKa = 11.84RR87 pKa = 11.84FRR89 pKa = 11.84IHH91 pKa = 7.0EE92 pKa = 4.4LPQLLNVLRR101 pKa = 11.84GEE103 pKa = 4.2MSLVGPGPEE112 pKa = 3.79LVDD115 pKa = 4.24RR116 pKa = 11.84VDD118 pKa = 3.86SLSGSDD124 pKa = 4.44KK125 pKa = 10.86RR126 pKa = 11.84LLNLRR131 pKa = 11.84PGMTDD136 pKa = 3.17WASLWNSDD144 pKa = 3.98EE145 pKa = 4.33ASSLAGAPDD154 pKa = 3.44RR155 pKa = 11.84EE156 pKa = 4.07LAYY159 pKa = 10.78DD160 pKa = 3.75RR161 pKa = 11.84FIRR164 pKa = 11.84PRR166 pKa = 11.84KK167 pKa = 9.49LRR169 pKa = 11.84LQKK172 pKa = 10.79YY173 pKa = 7.69YY174 pKa = 11.12LEE176 pKa = 4.25NRR178 pKa = 11.84SLGMDD183 pKa = 3.98LKK185 pKa = 10.79ILTFTVLRR193 pKa = 11.84VINRR197 pKa = 11.84QFLPRR202 pKa = 11.84EE203 pKa = 3.87LHH205 pKa = 5.74NFPTFGEE212 pKa = 4.0LRR214 pKa = 11.84AEE216 pKa = 4.31VLCLEE221 pKa = 4.32SSAMIRR227 pKa = 11.84KK228 pKa = 8.49HH229 pKa = 5.67AAA231 pKa = 2.83

Molecular weight: 26.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5127

0

5127

1840367

29

4569

359.0

39.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.891 ± 0.038

1.114 ± 0.014

6.137 ± 0.03

6.469 ± 0.045

3.488 ± 0.021

7.84 ± 0.047

2.158 ± 0.018

4.63 ± 0.021

3.312 ± 0.03

9.913 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.016

3.328 ± 0.027

5.101 ± 0.025

4.115 ± 0.026

5.986 ± 0.04

6.527 ± 0.033

5.932 ± 0.038

7.404 ± 0.031

1.608 ± 0.016

2.774 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski