Paenibacillus spiritus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J5GAQ8|A0A5J5GAQ8_9BACL Uncharacterized protein OS=Paenibacillus spiritus OX=2496557 GN=F4V43_12480 PE=4 SV=1
MM1 pKa = 7.01YY2 pKa = 10.11VVCKK6 pKa = 10.2EE7 pKa = 3.99HH8 pKa = 7.33LEE10 pKa = 3.97LAIDD14 pKa = 3.77QFVDD18 pKa = 3.92EE19 pKa = 5.47YY20 pKa = 11.1EE21 pKa = 4.5DD22 pKa = 4.29APDD25 pKa = 4.18VVDD28 pKa = 5.74LKK30 pKa = 10.84DD31 pKa = 3.61TEE33 pKa = 4.35FSDD36 pKa = 3.33WDD38 pKa = 3.95PPAKK42 pKa = 10.2CAEE45 pKa = 4.22CEE47 pKa = 4.06KK48 pKa = 10.24NAEE51 pKa = 4.19YY52 pKa = 10.83LVVV55 pKa = 3.5

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J5GAV4|A0A5J5GAV4_9BACL YheC/YheD family protein OS=Paenibacillus spiritus OX=2496557 GN=F4V43_08605 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 8.3VHH16 pKa = 5.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3778

0

3778

1245279

26

3715

329.6

36.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.662 ± 0.059

0.772 ± 0.013

5.079 ± 0.026

7.068 ± 0.054

3.688 ± 0.028

8.319 ± 0.048

1.853 ± 0.021

5.718 ± 0.039

4.564 ± 0.043

10.46 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.022

3.335 ± 0.035

4.39 ± 0.035

3.348 ± 0.025

6.232 ± 0.049

6.301 ± 0.04

5.187 ± 0.041

6.901 ± 0.039

1.183 ± 0.016

3.372 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski