Leptospirillum sp. Group IV UBA BS

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Leptospirillum; Leptospirillum sp. Group IV

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|T0Z678|T0Z678_9BACT Uncharacterized protein OS=Leptospirillum sp. Group IV 'UBA BS' OX=1260983 GN=D084_Lepto4C00422G0005 PE=4 SV=1
MM1 pKa = 7.89PSLLSWFLWRR11 pKa = 11.84NILDD15 pKa = 3.55ILAVWFIFYY24 pKa = 9.17QIPSPHH30 pKa = 6.47PTDD33 pKa = 4.88PGLPDD38 pKa = 4.35CLWVPVSFSSLLSDD52 pKa = 4.12GG53 pKa = 4.06

Molecular weight:
6.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|T0XND4|T0XND4_9BACT Hcy-binding domain-containing protein (Fragment) OS=Leptospirillum sp. Group IV 'UBA BS' OX=1260983 GN=D084_Lepto4C00467G0001 PE=4 SV=1
MM1 pKa = 7.6SLTFNPSNLRR11 pKa = 11.84RR12 pKa = 11.84TRR14 pKa = 11.84THH16 pKa = 6.36GFRR19 pKa = 11.84KK20 pKa = 10.05RR21 pKa = 11.84MATTAGKK28 pKa = 10.14KK29 pKa = 8.16VLKK32 pKa = 10.07KK33 pKa = 10.03RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84YY40 pKa = 8.53RR41 pKa = 11.84LTVV44 pKa = 2.95

Molecular weight:
5.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1878

0

1878

417274

21

1614

222.2

24.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.093 ± 0.061

0.846 ± 0.021

4.906 ± 0.04

6.584 ± 0.063

4.228 ± 0.039

8.759 ± 0.071

2.13 ± 0.026

5.247 ± 0.05

4.074 ± 0.059

11.081 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.026

2.645 ± 0.04

6.072 ± 0.057

2.65 ± 0.03

7.887 ± 0.082

7.061 ± 0.056

5.057 ± 0.047

6.909 ± 0.052

1.225 ± 0.024

2.276 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski