Leptospirillum sp. Group IV UBA BS
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1878 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0Z678|T0Z678_9BACT Uncharacterized protein OS=Leptospirillum sp. Group IV 'UBA BS' OX=1260983 GN=D084_Lepto4C00422G0005 PE=4 SV=1
MM1 pKa = 7.89 PSLLSWFLWRR11 pKa = 11.84 NILDD15 pKa = 3.55 ILAVWFIFYY24 pKa = 9.17 QIPSPHH30 pKa = 6.47 PTDD33 pKa = 4.88 PGLPDD38 pKa = 4.35 CLWVPVSFSSLLSDD52 pKa = 4.12 GG53 pKa = 4.06
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 4.19
IPC_protein 3.897
Toseland 3.706
ProMoST 4.177
Dawson 3.961
Bjellqvist 4.139
Wikipedia 4.024
Rodwell 3.757
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.139
DTASelect 4.418
Thurlkill 3.846
EMBOSS 4.024
Sillero 4.05
Patrickios 1.99
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|T0XND4|T0XND4_9BACT Hcy-binding domain-containing protein (Fragment) OS=Leptospirillum sp. Group IV 'UBA BS' OX=1260983 GN=D084_Lepto4C00467G0001 PE=4 SV=1
MM1 pKa = 7.6 SLTFNPSNLRR11 pKa = 11.84 RR12 pKa = 11.84 TRR14 pKa = 11.84 THH16 pKa = 6.36 GFRR19 pKa = 11.84 KK20 pKa = 10.05 RR21 pKa = 11.84 MATTAGKK28 pKa = 10.14 KK29 pKa = 8.16 VLKK32 pKa = 10.07 KK33 pKa = 10.03 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.05 GRR39 pKa = 11.84 YY40 pKa = 8.53 RR41 pKa = 11.84 LTVV44 pKa = 2.95
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1878
0
1878
417274
21
1614
222.2
24.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.093 ± 0.061
0.846 ± 0.021
4.906 ± 0.04
6.584 ± 0.063
4.228 ± 0.039
8.759 ± 0.071
2.13 ± 0.026
5.247 ± 0.05
4.074 ± 0.059
11.081 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.261 ± 0.026
2.645 ± 0.04
6.072 ± 0.057
2.65 ± 0.03
7.887 ± 0.082
7.061 ± 0.056
5.057 ± 0.047
6.909 ± 0.052
1.225 ± 0.024
2.276 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here