Allium cepa amalgavirus 2
Average proteome isoelectric point is 7.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5MGZ5|A0A2H5MGZ5_9VIRU ORF1+2p OS=Allium cepa amalgavirus 2 OX=2058779 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.62 ASSSGPDD8 pKa = 3.11 PTQYY12 pKa = 11.62 LDD14 pKa = 3.09 ITLLSDD20 pKa = 3.88 PVAEE24 pKa = 4.38 SVSLQDD30 pKa = 4.33 AVQHH34 pKa = 6.0 LASIGVRR41 pKa = 11.84 VEE43 pKa = 3.59 RR44 pKa = 11.84 FTRR47 pKa = 11.84 DD48 pKa = 2.87 SIHH51 pKa = 6.77 AMRR54 pKa = 11.84 MSVPAYY60 pKa = 9.43 VKK62 pKa = 10.04 EE63 pKa = 3.64 IRR65 pKa = 11.84 ILGNISDD72 pKa = 4.59 RR73 pKa = 11.84 EE74 pKa = 4.01 LLKK77 pKa = 11.05 NVMLQGVKK85 pKa = 10.78 NNVLSLPEE93 pKa = 4.53 DD94 pKa = 3.56 VTPQMVLSFARR105 pKa = 11.84 WLKK108 pKa = 9.84 RR109 pKa = 11.84 GAGARR114 pKa = 11.84 VLADD118 pKa = 3.35 QQHH121 pKa = 6.27 LLKK124 pKa = 10.42 INKK127 pKa = 9.06 KK128 pKa = 10.14 AVGDD132 pKa = 3.92 STPDD136 pKa = 2.91 IVAFAHH142 pKa = 7.2 LLDD145 pKa = 4.1 QQVQDD150 pKa = 3.73 LTNAKK155 pKa = 9.96 KK156 pKa = 10.16 RR157 pKa = 11.84 VQHH160 pKa = 6.03 SSQVRR165 pKa = 11.84 IDD167 pKa = 3.75 EE168 pKa = 4.08 LRR170 pKa = 11.84 KK171 pKa = 9.43 QIAIEE176 pKa = 3.87 EE177 pKa = 4.37 HH178 pKa = 5.47 NLRR181 pKa = 11.84 RR182 pKa = 11.84 DD183 pKa = 3.36 LAATARR189 pKa = 11.84 QFTPANDD196 pKa = 3.48 YY197 pKa = 11.22 APPSKK202 pKa = 10.57 AALDD206 pKa = 3.83 NEE208 pKa = 4.36 CWEE211 pKa = 4.83 LYY213 pKa = 9.72 CARR216 pKa = 11.84 ARR218 pKa = 11.84 DD219 pKa = 3.9 AGRR222 pKa = 11.84 ALPPWNAILQEE233 pKa = 4.09 QATTALSNEE242 pKa = 4.7 VINKK246 pKa = 8.53 HH247 pKa = 5.72 RR248 pKa = 11.84 QDD250 pKa = 3.17 FCKK253 pKa = 10.29 IPSNQRR259 pKa = 11.84 LLQTWAASKK268 pKa = 10.55 IQDD271 pKa = 3.47 LRR273 pKa = 11.84 TNQEE277 pKa = 3.6 FRR279 pKa = 11.84 RR280 pKa = 11.84 VTEE283 pKa = 4.04 FEE285 pKa = 4.17 RR286 pKa = 11.84 FLALASGDD294 pKa = 3.28 TSMASSSAPEE304 pKa = 4.04 AEE306 pKa = 4.42 MGQHH310 pKa = 6.98 DD311 pKa = 4.1 SGGEE315 pKa = 3.92 GGEE318 pKa = 4.15 SEE320 pKa = 4.35 EE321 pKa = 4.64 EE322 pKa = 4.17 EE323 pKa = 4.49 RR324 pKa = 11.84 EE325 pKa = 4.11 HH326 pKa = 6.13 TAAEE330 pKa = 4.26 HH331 pKa = 6.25 ARR333 pKa = 11.84 GGPSEE338 pKa = 4.21 QPDD341 pKa = 3.57 AEE343 pKa = 4.54 GDD345 pKa = 3.8 QEE347 pKa = 4.02 QTPARR352 pKa = 11.84 KK353 pKa = 9.3 KK354 pKa = 10.23 RR355 pKa = 11.84 RR356 pKa = 11.84 SKK358 pKa = 10.65 RR359 pKa = 11.84 LRR361 pKa = 11.84 NFPSRR366 pKa = 11.84 HH367 pKa = 4.39 QRR369 pKa = 11.84 SHH371 pKa = 6.25 SGSKK375 pKa = 9.16 KK376 pKa = 9.54 HH377 pKa = 6.09 RR378 pKa = 11.84 KK379 pKa = 8.65 KK380 pKa = 10.46 PNRR383 pKa = 11.84 RR384 pKa = 11.84 DD385 pKa = 3.26 SDD387 pKa = 3.71 GQEE390 pKa = 3.6
Molecular weight: 43.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.532
IPC2_protein 7.775
IPC_protein 7.805
Toseland 8.083
ProMoST 8.244
Dawson 8.58
Bjellqvist 8.653
Wikipedia 8.536
Rodwell 8.639
Grimsley 7.966
Solomon 8.799
Lehninger 8.799
Nozaki 8.931
DTASelect 8.521
Thurlkill 8.653
EMBOSS 8.814
Sillero 8.873
Patrickios 5.308
IPC_peptide 8.799
IPC2_peptide 7.497
IPC2.peptide.svr19 7.468
Protein with the highest isoelectric point:
>tr|A0A2H5MGZ5|A0A2H5MGZ5_9VIRU ORF1+2p OS=Allium cepa amalgavirus 2 OX=2058779 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.62 ASSSGPDD8 pKa = 3.11 PTQYY12 pKa = 11.62 LDD14 pKa = 3.09 ITLLSDD20 pKa = 3.88 PVAEE24 pKa = 4.38 SVSLQDD30 pKa = 4.33 AVQHH34 pKa = 6.0 LASIGVRR41 pKa = 11.84 VEE43 pKa = 3.59 RR44 pKa = 11.84 FTRR47 pKa = 11.84 DD48 pKa = 2.87 SIHH51 pKa = 6.77 AMRR54 pKa = 11.84 MSVPAYY60 pKa = 9.43 VKK62 pKa = 10.04 EE63 pKa = 3.64 IRR65 pKa = 11.84 ILGNISDD72 pKa = 4.59 RR73 pKa = 11.84 EE74 pKa = 4.01 LLKK77 pKa = 11.05 NVMLQGVKK85 pKa = 10.78 NNVLSLPEE93 pKa = 4.53 DD94 pKa = 3.56 VTPQMVLSFARR105 pKa = 11.84 WLKK108 pKa = 9.84 RR109 pKa = 11.84 GAGARR114 pKa = 11.84 VLADD118 pKa = 3.35 QQHH121 pKa = 6.27 LLKK124 pKa = 10.42 INKK127 pKa = 9.06 KK128 pKa = 10.14 AVGDD132 pKa = 3.92 STPDD136 pKa = 2.91 IVAFAHH142 pKa = 7.2 LLDD145 pKa = 4.1 QQVQDD150 pKa = 3.73 LTNAKK155 pKa = 9.96 KK156 pKa = 10.16 RR157 pKa = 11.84 VQHH160 pKa = 6.03 SSQVRR165 pKa = 11.84 IDD167 pKa = 3.75 EE168 pKa = 4.08 LRR170 pKa = 11.84 KK171 pKa = 9.43 QIAIEE176 pKa = 3.87 EE177 pKa = 4.37 HH178 pKa = 5.47 NLRR181 pKa = 11.84 RR182 pKa = 11.84 DD183 pKa = 3.36 LAATARR189 pKa = 11.84 QFTPANDD196 pKa = 3.48 YY197 pKa = 11.22 APPSKK202 pKa = 10.57 AALDD206 pKa = 3.83 NEE208 pKa = 4.36 CWEE211 pKa = 4.83 LYY213 pKa = 9.72 CARR216 pKa = 11.84 ARR218 pKa = 11.84 DD219 pKa = 3.9 AGRR222 pKa = 11.84 ALPPWNAILQEE233 pKa = 4.09 QATTALSNEE242 pKa = 4.7 VINKK246 pKa = 8.53 HH247 pKa = 5.72 RR248 pKa = 11.84 QDD250 pKa = 3.17 FCKK253 pKa = 10.29 IPSNQRR259 pKa = 11.84 LLQTWAASKK268 pKa = 10.55 IQDD271 pKa = 3.47 LRR273 pKa = 11.84 TNQEE277 pKa = 3.95 FVASQSSNDD286 pKa = 3.03 SWLSQVEE293 pKa = 4.12 THH295 pKa = 6.42 LWRR298 pKa = 11.84 LPLPLRR304 pKa = 11.84 LKK306 pKa = 8.91 WANTIPVGKK315 pKa = 9.98 VVSRR319 pKa = 11.84 KK320 pKa = 9.26 RR321 pKa = 11.84 RR322 pKa = 11.84 NGNILLRR329 pKa = 11.84 SMLGADD335 pKa = 4.28 LLNNPMQRR343 pKa = 11.84 GIKK346 pKa = 9.71 NKK348 pKa = 9.96 PPLARR353 pKa = 11.84 NAEE356 pKa = 4.12 VSASVTSLLDD366 pKa = 3.04 INARR370 pKa = 11.84 ILVLRR375 pKa = 11.84 SIEE378 pKa = 3.93 RR379 pKa = 11.84 SPTVGIPTARR389 pKa = 11.84 SRR391 pKa = 11.84 FEE393 pKa = 3.61 GAVRR397 pKa = 11.84 KK398 pKa = 10.18 VIGGGEE404 pKa = 4.12 MIDD407 pKa = 3.02 WRR409 pKa = 11.84 TVSNQYY415 pKa = 10.46 RR416 pKa = 11.84 GGGCFSDD423 pKa = 5.76 AILLLADD430 pKa = 4.07 ARR432 pKa = 11.84 TDD434 pKa = 3.49 EE435 pKa = 4.78 PGKK438 pKa = 10.29 FLPDD442 pKa = 3.85 YY443 pKa = 7.85 FTLWKK448 pKa = 10.7 ARR450 pKa = 11.84 DD451 pKa = 3.7 ILRR454 pKa = 11.84 LPSDD458 pKa = 4.01 LKK460 pKa = 11.23 VPCNRR465 pKa = 11.84 QALKK469 pKa = 10.9 VSNFNNDD476 pKa = 2.37 ATAGPFFRR484 pKa = 11.84 AYY486 pKa = 10.22 GIKK489 pKa = 9.94 SKK491 pKa = 11.34 YY492 pKa = 9.28 GMRR495 pKa = 11.84 GLLEE499 pKa = 4.27 DD500 pKa = 4.93 FAWEE504 pKa = 4.76 CYY506 pKa = 10.41 SSFVDD511 pKa = 3.49 NGGDD515 pKa = 3.48 VSFLPFVASRR525 pKa = 11.84 VGFRR529 pKa = 11.84 TKK531 pKa = 10.5 LVSQEE536 pKa = 3.55 EE537 pKa = 4.13 AFIRR541 pKa = 11.84 FSKK544 pKa = 10.62 NKK546 pKa = 10.31 AIGRR550 pKa = 11.84 CVMMLDD556 pKa = 4.91 AIEE559 pKa = 4.28 QMFSSPLYY567 pKa = 10.21 NVLSKK572 pKa = 10.01 LTADD576 pKa = 4.45 LRR578 pKa = 11.84 FDD580 pKa = 3.75 PASGFRR586 pKa = 11.84 NTIVRR591 pKa = 11.84 ASSDD595 pKa = 3.05 WAKK598 pKa = 9.69 MWEE601 pKa = 4.3 EE602 pKa = 3.87 VKK604 pKa = 10.39 KK605 pKa = 10.72 AQVIVEE611 pKa = 4.28 LDD613 pKa = 2.9 WKK615 pKa = 10.85 KK616 pKa = 10.69 FDD618 pKa = 4.23 RR619 pKa = 11.84 EE620 pKa = 4.13 RR621 pKa = 11.84 PTEE624 pKa = 4.25 DD625 pKa = 3.38 LAFMIDD631 pKa = 3.86 VIISCFKK638 pKa = 10.43 PEE640 pKa = 3.94 NDD642 pKa = 3.7 RR643 pKa = 11.84 EE644 pKa = 4.18 RR645 pKa = 11.84 LFLLAYY651 pKa = 7.69 KK652 pKa = 10.61 TMMQRR657 pKa = 11.84 CLIEE661 pKa = 4.33 RR662 pKa = 11.84 FFVTDD667 pKa = 3.34 CGGVFKK673 pKa = 11.33 VEE675 pKa = 4.04 GMVPSGSLWTGWVDD689 pKa = 3.2 TALNILYY696 pKa = 10.45 LNAALLSLGFNMTEE710 pKa = 4.18 VFPKK714 pKa = 10.71 CAGDD718 pKa = 4.12 DD719 pKa = 3.7 NLTLFMRR726 pKa = 11.84 DD727 pKa = 3.04 VEE729 pKa = 4.26 NRR731 pKa = 11.84 RR732 pKa = 11.84 LLALKK737 pKa = 10.44 DD738 pKa = 3.81 RR739 pKa = 11.84 LNSWFRR745 pKa = 11.84 AGIEE749 pKa = 4.07 AEE751 pKa = 4.38 DD752 pKa = 4.26 FLIHH756 pKa = 6.84 RR757 pKa = 11.84 PPFFVTRR764 pKa = 11.84 EE765 pKa = 3.72 QAVFPRR771 pKa = 11.84 GTDD774 pKa = 3.45 LTKK777 pKa = 10.18 GTSKK781 pKa = 10.73 IIGNAVWVPIEE792 pKa = 4.08 GEE794 pKa = 4.08 MVIDD798 pKa = 3.64 QEE800 pKa = 4.62 AGRR803 pKa = 11.84 SHH805 pKa = 6.87 RR806 pKa = 11.84 WQYY809 pKa = 9.78 VFRR812 pKa = 11.84 GRR814 pKa = 11.84 PKK816 pKa = 10.24 FLSAYY821 pKa = 7.8 WLEE824 pKa = 4.52 DD825 pKa = 3.06 GRR827 pKa = 11.84 PIRR830 pKa = 11.84 PTHH833 pKa = 6.13 INSEE837 pKa = 4.08 KK838 pKa = 10.75 LLFPEE843 pKa = 6.17 GIHH846 pKa = 7.39 DD847 pKa = 5.6 SIDD850 pKa = 3.47 TYY852 pKa = 11.03 EE853 pKa = 4.18 AAVLSMVVDD862 pKa = 3.74 NPFNHH867 pKa = 6.78 HH868 pKa = 6.66 NINHH872 pKa = 5.59 CMHH875 pKa = 6.94 RR876 pKa = 11.84 FVICEE881 pKa = 3.66 QVKK884 pKa = 9.08 RR885 pKa = 11.84 QARR888 pKa = 11.84 MGLDD892 pKa = 3.97 PIDD895 pKa = 4.17 ILWLSRR901 pKa = 11.84 FRR903 pKa = 11.84 SSVGEE908 pKa = 3.91 EE909 pKa = 3.93 VPYY912 pKa = 10.93 PMIASWRR919 pKa = 11.84 RR920 pKa = 11.84 QDD922 pKa = 2.73 TWVDD926 pKa = 3.43 MEE928 pKa = 4.13 QLPFVRR934 pKa = 11.84 DD935 pKa = 3.42 YY936 pKa = 11.76 VRR938 pKa = 11.84 DD939 pKa = 3.58 FRR941 pKa = 11.84 EE942 pKa = 4.13 FVSGVTSLYY951 pKa = 10.63 SRR953 pKa = 11.84 QSTGGLDD960 pKa = 3.05 SWKK963 pKa = 8.51 FTSIIRR969 pKa = 11.84 GEE971 pKa = 4.35 TEE973 pKa = 3.53 FGEE976 pKa = 4.67 GQFGNDD982 pKa = 2.67 IDD984 pKa = 5.63 DD985 pKa = 4.76 WISWMYY991 pKa = 9.88 RR992 pKa = 11.84 NPMTKK997 pKa = 8.74 YY998 pKa = 9.55 LRR1000 pKa = 11.84 PIRR1003 pKa = 11.84 RR1004 pKa = 11.84 FRR1006 pKa = 11.84 AVGEE1010 pKa = 3.94 EE1011 pKa = 3.86 VRR1013 pKa = 11.84 PDD1015 pKa = 3.51 RR1016 pKa = 11.84 EE1017 pKa = 4.17 LSGSLQRR1024 pKa = 11.84 CIGIYY1029 pKa = 9.41 RR1030 pKa = 11.84 QIRR1033 pKa = 11.84 GTQEE1037 pKa = 3.5 NSSSEE1042 pKa = 3.78 NFAIFLSNVLRR1053 pKa = 11.84 QTRR1056 pKa = 11.84 SYY1058 pKa = 11.09 RR1059 pKa = 11.84 DD1060 pKa = 3.55 ANAHH1064 pKa = 5.57 PP1065 pKa = 4.36
Molecular weight: 121.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.387
IPC2_protein 8.302
IPC_protein 8.185
Toseland 8.639
ProMoST 8.77
Dawson 9.107
Bjellqvist 9.121
Wikipedia 9.209
Rodwell 9.238
Grimsley 8.931
Solomon 9.165
Lehninger 9.136
Nozaki 9.282
DTASelect 8.96
Thurlkill 9.063
EMBOSS 9.268
Sillero 9.282
Patrickios 4.952
IPC_peptide 9.165
IPC2_peptide 7.863
IPC2.peptide.svr19 7.714
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1455
390
1065
727.5
82.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.247 ± 1.152
1.168 ± 0.203
6.323 ± 0.044
5.979 ± 0.741
4.261 ± 0.993
5.361 ± 0.249
2.062 ± 0.647
5.223 ± 0.7
4.674 ± 0.362
9.21 ± 0.38
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.276
4.605 ± 0.255
4.742 ± 0.066
4.811 ± 1.074
9.003 ± 0.116
7.629 ± 0.423
4.192 ± 0.046
6.392 ± 0.643
1.993 ± 0.492
1.787 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here