Amino acid dipepetide frequency for Ancylobacter pratisalsi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.417AlaAla: 18.417 ± 0.165
1.064AlaCys: 1.064 ± 0.028
6.924AlaAsp: 6.924 ± 0.083
7.541AlaGlu: 7.541 ± 0.095
4.783AlaPhe: 4.783 ± 0.066
11.855AlaGly: 11.855 ± 0.111
2.44AlaHis: 2.44 ± 0.039
6.419AlaIle: 6.419 ± 0.068
3.605AlaLys: 3.605 ± 0.056
14.936AlaLeu: 14.936 ± 0.138
3.512AlaMet: 3.512 ± 0.051
2.692AlaAsn: 2.692 ± 0.051
6.682AlaPro: 6.682 ± 0.097
3.888AlaGln: 3.888 ± 0.058
10.348AlaArg: 10.348 ± 0.113
6.785AlaSer: 6.785 ± 0.073
6.354AlaThr: 6.354 ± 0.068
9.285AlaVal: 9.285 ± 0.095
1.488AlaTrp: 1.488 ± 0.032
2.638AlaTyr: 2.638 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.014CysAla: 1.014 ± 0.029
0.105CysCys: 0.105 ± 0.01
0.46CysAsp: 0.46 ± 0.018
0.396CysGlu: 0.396 ± 0.018
0.318CysPhe: 0.318 ± 0.017
0.856CysGly: 0.856 ± 0.028
0.209CysHis: 0.209 ± 0.013
0.365CysIle: 0.365 ± 0.017
0.146CysLys: 0.146 ± 0.011
0.805CysLeu: 0.805 ± 0.026
0.146CysMet: 0.146 ± 0.01
0.159CysAsn: 0.159 ± 0.011
0.412CysPro: 0.412 ± 0.022
0.184CysGln: 0.184 ± 0.012
0.602CysArg: 0.602 ± 0.021
0.383CysSer: 0.383 ± 0.017
0.388CysThr: 0.388 ± 0.02
0.617CysVal: 0.617 ± 0.021
0.106CysTrp: 0.106 ± 0.01
0.187CysTyr: 0.187 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.11AspAla: 7.11 ± 0.072
0.425AspCys: 0.425 ± 0.018
2.886AspAsp: 2.886 ± 0.049
3.484AspGlu: 3.484 ± 0.059
2.072AspPhe: 2.072 ± 0.042
5.028AspGly: 5.028 ± 0.071
1.217AspHis: 1.217 ± 0.032
3.026AspIle: 3.026 ± 0.05
1.444AspLys: 1.444 ± 0.037
5.659AspLeu: 5.659 ± 0.071
1.27AspMet: 1.27 ± 0.03
1.118AspAsn: 1.118 ± 0.032
3.597AspPro: 3.597 ± 0.054
1.396AspGln: 1.396 ± 0.034
4.033AspArg: 4.033 ± 0.055
2.03AspSer: 2.03 ± 0.038
2.624AspThr: 2.624 ± 0.048
4.312AspVal: 4.312 ± 0.065
0.919AspTrp: 0.919 ± 0.023
1.394AspTyr: 1.394 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
8.078GluAla: 8.078 ± 0.093
0.341GluCys: 0.341 ± 0.015
2.624GluAsp: 2.624 ± 0.046
3.12GluGlu: 3.12 ± 0.061
1.599GluPhe: 1.599 ± 0.039
4.759GluGly: 4.759 ± 0.061
1.197GluHis: 1.197 ± 0.033
3.3GluIle: 3.3 ± 0.048
1.88GluLys: 1.88 ± 0.048
5.431GluLeu: 5.431 ± 0.062
1.526GluMet: 1.526 ± 0.031
1.368GluAsn: 1.368 ± 0.035
2.928GluPro: 2.928 ± 0.057
1.867GluGln: 1.867 ± 0.042
5.189GluArg: 5.189 ± 0.083
2.261GluSer: 2.261 ± 0.036
3.226GluThr: 3.226 ± 0.051
4.099GluVal: 4.099 ± 0.054
0.68GluTrp: 0.68 ± 0.021
0.85GluTyr: 0.85 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.804PheAla: 4.804 ± 0.07
0.338PheCys: 0.338 ± 0.014
2.55PheAsp: 2.55 ± 0.047
2.137PheGlu: 2.137 ± 0.04
1.384PhePhe: 1.384 ± 0.037
3.882PheGly: 3.882 ± 0.063
0.761PheHis: 0.761 ± 0.026
1.738PheIle: 1.738 ± 0.034
0.868PheLys: 0.868 ± 0.029
3.401PheLeu: 3.401 ± 0.06
0.814PheMet: 0.814 ± 0.03
0.938PheAsn: 0.938 ± 0.027
1.672PhePro: 1.672 ± 0.039
0.956PheGln: 0.956 ± 0.026
2.263PheArg: 2.263 ± 0.047
2.09PheSer: 2.09 ± 0.042
2.028PheThr: 2.028 ± 0.044
2.921PheVal: 2.921 ± 0.049
0.543PheTrp: 0.543 ± 0.023
0.852PheTyr: 0.852 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
10.452GlyAla: 10.452 ± 0.097
0.812GlyCys: 0.812 ± 0.026
4.115GlyAsp: 4.115 ± 0.061
5.064GlyGlu: 5.064 ± 0.071
3.743GlyPhe: 3.743 ± 0.059
7.647GlyGly: 7.647 ± 0.112
1.986GlyHis: 1.986 ± 0.043
4.673GlyIle: 4.673 ± 0.057
2.768GlyLys: 2.768 ± 0.054
9.369GlyLeu: 9.369 ± 0.099
2.294GlyMet: 2.294 ± 0.045
1.952GlyAsn: 1.952 ± 0.045
3.94GlyPro: 3.94 ± 0.06
2.809GlyGln: 2.809 ± 0.049
6.813GlyArg: 6.813 ± 0.081
4.575GlySer: 4.575 ± 0.061
5.006GlyThr: 5.006 ± 0.07
6.709GlyVal: 6.709 ± 0.075
1.444GlyTrp: 1.444 ± 0.03
2.278GlyTyr: 2.278 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.544HisAla: 2.544 ± 0.039
0.203HisCys: 0.203 ± 0.012
1.192HisAsp: 1.192 ± 0.034
1.078HisGlu: 1.078 ± 0.031
0.828HisPhe: 0.828 ± 0.025
1.987HisGly: 1.987 ± 0.042
0.547HisHis: 0.547 ± 0.023
0.93HisIle: 0.93 ± 0.026
0.43HisLys: 0.43 ± 0.018
2.078HisLeu: 2.078 ± 0.039
0.51HisMet: 0.51 ± 0.019
0.423HisAsn: 0.423 ± 0.018
1.344HisPro: 1.344 ± 0.036
0.538HisGln: 0.538 ± 0.017
1.438HisArg: 1.438 ± 0.031
0.896HisSer: 0.896 ± 0.028
0.812HisThr: 0.812 ± 0.024
1.623HisVal: 1.623 ± 0.031
0.324HisTrp: 0.324 ± 0.017
0.496HisTyr: 0.496 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.437IleAla: 7.437 ± 0.071
0.459IleCys: 0.459 ± 0.018
3.582IleAsp: 3.582 ± 0.054
3.615IleGlu: 3.615 ± 0.053
1.595IlePhe: 1.595 ± 0.037
5.223IleGly: 5.223 ± 0.06
0.856IleHis: 0.856 ± 0.027
2.181IleIle: 2.181 ± 0.049
1.226IleLys: 1.226 ± 0.034
4.287IleLeu: 4.287 ± 0.062
0.953IleMet: 0.953 ± 0.028
1.25IleAsn: 1.25 ± 0.03
2.24IlePro: 2.24 ± 0.038
1.096IleGln: 1.096 ± 0.029
2.982IleArg: 2.982 ± 0.047
2.631IleSer: 2.631 ± 0.042
2.611IleThr: 2.611 ± 0.044
4.25IleVal: 4.25 ± 0.056
0.549IleTrp: 0.549 ± 0.021
1.116IleTyr: 1.116 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.758LysAla: 3.758 ± 0.052
0.124LysCys: 0.124 ± 0.009
1.474LysAsp: 1.474 ± 0.04
1.35LysGlu: 1.35 ± 0.034
0.687LysPhe: 0.687 ± 0.026
2.376LysGly: 2.376 ± 0.044
0.483LysHis: 0.483 ± 0.02
1.368LysIle: 1.368 ± 0.034
1.024LysLys: 1.024 ± 0.035
2.931LysLeu: 2.931 ± 0.045
0.662LysMet: 0.662 ± 0.023
0.721LysAsn: 0.721 ± 0.023
1.749LysPro: 1.749 ± 0.039
0.83LysGln: 0.83 ± 0.025
2.019LysArg: 2.019 ± 0.043
1.44LysSer: 1.44 ± 0.035
1.572LysThr: 1.572 ± 0.039
2.358LysVal: 2.358 ± 0.048
0.335LysTrp: 0.335 ± 0.017
0.486LysTyr: 0.486 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.466LeuAla: 15.466 ± 0.132
0.817LeuCys: 0.817 ± 0.026
6.418LeuAsp: 6.418 ± 0.075
5.041LeuGlu: 5.041 ± 0.071
3.74LeuPhe: 3.74 ± 0.064
8.909LeuGly: 8.909 ± 0.087
1.825LeuHis: 1.825 ± 0.033
5.146LeuIle: 5.146 ± 0.068
3.214LeuLys: 3.214 ± 0.056
9.764LeuLeu: 9.764 ± 0.116
2.493LeuMet: 2.493 ± 0.052
2.354LeuAsn: 2.354 ± 0.039
5.991LeuPro: 5.991 ± 0.08
2.426LeuGln: 2.426 ± 0.042
6.958LeuArg: 6.958 ± 0.079
6.367LeuSer: 6.367 ± 0.073
5.62LeuThr: 5.62 ± 0.067
8.363LeuVal: 8.363 ± 0.089
1.172LeuTrp: 1.172 ± 0.034
2.035LeuTyr: 2.035 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.036MetAla: 3.036 ± 0.044
0.16MetCys: 0.16 ± 0.01
1.088MetAsp: 1.088 ± 0.028
1.126MetGlu: 1.126 ± 0.028
0.744MetPhe: 0.744 ± 0.025
1.705MetGly: 1.705 ± 0.04
0.417MetHis: 0.417 ± 0.019
1.243MetIle: 1.243 ± 0.033
0.886MetLys: 0.886 ± 0.028
2.597MetLeu: 2.597 ± 0.041
0.668MetMet: 0.668 ± 0.024
0.774MetAsn: 0.774 ± 0.024
1.572MetPro: 1.572 ± 0.034
0.694MetGln: 0.694 ± 0.021
1.876MetArg: 1.876 ± 0.036
1.758MetSer: 1.758 ± 0.041
1.616MetThr: 1.616 ± 0.035
1.794MetVal: 1.794 ± 0.035
0.225MetTrp: 0.225 ± 0.013
0.308MetTyr: 0.308 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.909AsnAla: 2.909 ± 0.046
0.195AsnCys: 0.195 ± 0.012
1.209AsnAsp: 1.209 ± 0.028
1.16AsnGlu: 1.16 ± 0.028
0.847AsnPhe: 0.847 ± 0.024
2.181AsnGly: 2.181 ± 0.043
0.46AsnHis: 0.46 ± 0.018
1.194AsnIle: 1.194 ± 0.033
0.563AsnLys: 0.563 ± 0.023
2.363AsnLeu: 2.363 ± 0.042
0.539AsnMet: 0.539 ± 0.02
0.57AsnAsn: 0.57 ± 0.022
1.651AsnPro: 1.651 ± 0.038
0.582AsnGln: 0.582 ± 0.02
1.637AsnArg: 1.637 ± 0.033
1.041AsnSer: 1.041 ± 0.03
1.108AsnThr: 1.108 ± 0.033
1.934AsnVal: 1.934 ± 0.037
0.433AsnTrp: 0.433 ± 0.018
0.593AsnTyr: 0.593 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.468ProAla: 7.468 ± 0.104
0.289ProCys: 0.289 ± 0.016
3.695ProAsp: 3.695 ± 0.061
3.813ProGlu: 3.813 ± 0.058
2.103ProPhe: 2.103 ± 0.041
5.032ProGly: 5.032 ± 0.059
1.119ProHis: 1.119 ± 0.027
2.345ProIle: 2.345 ± 0.042
1.439ProLys: 1.439 ± 0.038
5.203ProLeu: 5.203 ± 0.064
1.269ProMet: 1.269 ± 0.032
1.328ProAsn: 1.328 ± 0.033
3.062ProPro: 3.062 ± 0.076
1.559ProGln: 1.559 ± 0.032
3.36ProArg: 3.36 ± 0.051
3.077ProSer: 3.077 ± 0.05
2.734ProThr: 2.734 ± 0.049
4.445ProVal: 4.445 ± 0.054
0.694ProTrp: 0.694 ± 0.021
1.187ProTyr: 1.187 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.75GlnAla: 3.75 ± 0.06
0.187GlnCys: 0.187 ± 0.013
1.284GlnAsp: 1.284 ± 0.029
1.32GlnGlu: 1.32 ± 0.029
0.968GlnPhe: 0.968 ± 0.023
2.191GlnGly: 2.191 ± 0.042
0.579GlnHis: 0.579 ± 0.02
1.611GlnIle: 1.611 ± 0.035
0.856GlnLys: 0.856 ± 0.025
2.759GlnLeu: 2.759 ± 0.047
0.8GlnMet: 0.8 ± 0.022
0.738GlnAsn: 0.738 ± 0.024
1.61GlnPro: 1.61 ± 0.037
1.057GlnGln: 1.057 ± 0.035
2.265GlnArg: 2.265 ± 0.041
1.493GlnSer: 1.493 ± 0.032
1.489GlnThr: 1.489 ± 0.039
2.11GlnVal: 2.11 ± 0.041
0.384GlnTrp: 0.384 ± 0.016
0.498GlnTyr: 0.498 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.049ArgAla: 9.049 ± 0.107
0.537ArgCys: 0.537 ± 0.021
3.88ArgAsp: 3.88 ± 0.053
4.374ArgGlu: 4.374 ± 0.065
2.994ArgPhe: 2.994 ± 0.042
5.275ArgGly: 5.275 ± 0.066
1.81ArgHis: 1.81 ± 0.042
3.888ArgIle: 3.888 ± 0.054
1.779ArgLys: 1.779 ± 0.038
8.669ArgLeu: 8.669 ± 0.088
1.816ArgMet: 1.816 ± 0.031
1.579ArgAsn: 1.579 ± 0.037
4.071ArgPro: 4.071 ± 0.073
2.32ArgGln: 2.32 ± 0.04
6.339ArgArg: 6.339 ± 0.095
3.676ArgSer: 3.676 ± 0.061
3.555ArgThr: 3.555 ± 0.053
5.324ArgVal: 5.324 ± 0.055
0.978ArgTrp: 0.978 ± 0.027
1.616ArgTyr: 1.616 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.28SerAla: 6.28 ± 0.064
0.393SerCys: 0.393 ± 0.015
2.726SerAsp: 2.726 ± 0.046
2.687SerGlu: 2.687 ± 0.046
2.294SerPhe: 2.294 ± 0.04
5.454SerGly: 5.454 ± 0.076
1.071SerHis: 1.071 ± 0.028
2.597SerIle: 2.597 ± 0.045
1.307SerLys: 1.307 ± 0.03
5.487SerLeu: 5.487 ± 0.07
1.252SerMet: 1.252 ± 0.034
1.231SerAsn: 1.231 ± 0.028
2.965SerPro: 2.965 ± 0.05
1.391SerGln: 1.391 ± 0.033
3.689SerArg: 3.689 ± 0.056
2.947SerSer: 2.947 ± 0.051
2.771SerThr: 2.771 ± 0.039
4.039SerVal: 4.039 ± 0.058
0.743SerTrp: 0.743 ± 0.023
1.262SerTyr: 1.262 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.17ThrAla: 6.17 ± 0.062
0.408ThrCys: 0.408 ± 0.017
2.654ThrAsp: 2.654 ± 0.046
2.49ThrGlu: 2.49 ± 0.042
2.029ThrPhe: 2.029 ± 0.037
5.011ThrGly: 5.011 ± 0.062
1.07ThrHis: 1.07 ± 0.026
2.699ThrIle: 2.699 ± 0.046
1.243ThrLys: 1.243 ± 0.032
6.301ThrLeu: 6.301 ± 0.071
1.126ThrMet: 1.126 ± 0.03
1.178ThrAsn: 1.178 ± 0.03
3.515ThrPro: 3.515 ± 0.057
1.371ThrGln: 1.371 ± 0.03
3.555ThrArg: 3.555 ± 0.052
2.889ThrSer: 2.889 ± 0.048
2.837ThrThr: 2.837 ± 0.052
4.285ThrVal: 4.285 ± 0.056
0.624ThrTrp: 0.624 ± 0.025
1.222ThrTyr: 1.222 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.137ValAla: 10.137 ± 0.096
0.635ValCys: 0.635 ± 0.022
4.352ValAsp: 4.352 ± 0.057
4.737ValGlu: 4.737 ± 0.061
2.832ValPhe: 2.832 ± 0.054
6.101ValGly: 6.101 ± 0.071
1.455ValHis: 1.455 ± 0.03
3.937ValIle: 3.937 ± 0.052
2.103ValLys: 2.103 ± 0.041
8.233ValLeu: 8.233 ± 0.092
1.897ValMet: 1.897 ± 0.041
1.83ValAsn: 1.83 ± 0.034
4.397ValPro: 4.397 ± 0.058
1.853ValGln: 1.853 ± 0.037
5.339ValArg: 5.339 ± 0.068
4.271ValSer: 4.271 ± 0.055
4.553ValThr: 4.553 ± 0.054
6.538ValVal: 6.538 ± 0.094
0.907ValTrp: 0.907 ± 0.028
1.508ValTyr: 1.508 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.259TrpAla: 1.259 ± 0.034
0.142TrpCys: 0.142 ± 0.011
0.573TrpAsp: 0.573 ± 0.022
0.571TrpGlu: 0.571 ± 0.019
0.522TrpPhe: 0.522 ± 0.017
0.904TrpGly: 0.904 ± 0.029
0.318TrpHis: 0.318 ± 0.015
0.673TrpIle: 0.673 ± 0.023
0.441TrpLys: 0.441 ± 0.019
1.639TrpLeu: 1.639 ± 0.039
0.327TrpMet: 0.327 ± 0.015
0.448TrpAsn: 0.448 ± 0.021
0.709TrpPro: 0.709 ± 0.023
0.509TrpGln: 0.509 ± 0.02
1.172TrpArg: 1.172 ± 0.03
0.829TrpSer: 0.829 ± 0.025
0.734TrpThr: 0.734 ± 0.022
0.832TrpVal: 0.832 ± 0.024
0.23TrpTrp: 0.23 ± 0.013
0.277TrpTyr: 0.277 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.041
0.222TyrCys: 0.222 ± 0.013
1.358TyrAsp: 1.358 ± 0.032
1.221TyrGlu: 1.221 ± 0.028
0.86TyrPhe: 0.86 ± 0.025
2.075TyrGly: 2.075 ± 0.04
0.402TyrHis: 0.402 ± 0.017
0.806TyrIle: 0.806 ± 0.025
0.526TyrLys: 0.526 ± 0.021
2.205TyrLeu: 2.205 ± 0.042
0.414TyrMet: 0.414 ± 0.017
0.517TyrAsn: 0.517 ± 0.021
1.115TyrPro: 1.115 ± 0.028
0.621TyrGln: 0.621 ± 0.024
1.653TyrArg: 1.653 ± 0.035
1.148TyrSer: 1.148 ± 0.031
1.039TyrThr: 1.039 ± 0.031
1.776TyrVal: 1.776 ± 0.037
0.33TyrTrp: 0.33 ± 0.016
0.567TyrTyr: 0.567 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4319 proteins (1392111 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski