Cyphellophora europaea CBS 101466

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Cyphellophoraceae; Cyphellophora; Cyphellophora europaea

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11090 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2RLC9|W2RLC9_9EURO Uncharacterized protein OS=Cyphellophora europaea CBS 101466 OX=1220924 GN=HMPREF1541_08812 PE=4 SV=1
MM1 pKa = 7.62APGIPTAVSADD12 pKa = 3.6HH13 pKa = 6.58QPTKK17 pKa = 8.74TVRR20 pKa = 11.84IADD23 pKa = 4.15DD24 pKa = 5.01GEE26 pKa = 4.39DD27 pKa = 3.59TMQDD31 pKa = 3.2AQVSPLQSAYY41 pKa = 8.79EE42 pKa = 4.08RR43 pKa = 11.84KK44 pKa = 9.9ADD46 pKa = 3.63AVKK49 pKa = 9.26EE50 pKa = 4.11TVIRR54 pKa = 11.84GGRR57 pKa = 11.84PGVVDD62 pKa = 4.25NGSSDD67 pKa = 3.14IPSTYY72 pKa = 10.55KK73 pKa = 10.62PVDD76 pKa = 3.5EE77 pKa = 6.23DD78 pKa = 4.51DD79 pKa = 5.79DD80 pKa = 4.76SEE82 pKa = 4.32QVAPYY87 pKa = 10.38FPDD90 pKa = 3.07QDD92 pKa = 3.9ANKK95 pKa = 7.34PTTWGFHH102 pKa = 6.24ILDD105 pKa = 3.8NNQPGFAVDD114 pKa = 3.55NAVTEE119 pKa = 4.09QAEE122 pKa = 4.44DD123 pKa = 4.17GDD125 pKa = 5.17DD126 pKa = 4.68DD127 pKa = 5.13SQATPNNSLGDD138 pKa = 3.86DD139 pKa = 4.01TDD141 pKa = 3.65THH143 pKa = 6.06HH144 pKa = 7.59TDD146 pKa = 3.03AQVEE150 pKa = 4.33GGVDD154 pKa = 3.54GGDD157 pKa = 3.67GQNGDD162 pKa = 3.99QEE164 pKa = 5.52AGDD167 pKa = 4.69DD168 pKa = 4.14GQAVTDD174 pKa = 4.1SVAADD179 pKa = 3.55VAAQAAAADD188 pKa = 4.14ADD190 pKa = 4.26STQNWLVAQDD200 pKa = 3.0SWLNQLAAEE209 pKa = 4.28NPEE212 pKa = 3.78EE213 pKa = 4.07FARR216 pKa = 11.84LVEE219 pKa = 4.41SGVVDD224 pKa = 3.73PAFIPHH230 pKa = 7.15AAAQEE235 pKa = 4.37NIDD238 pKa = 3.97GDD240 pKa = 4.01GGADD244 pKa = 3.51GNTTATGAEE253 pKa = 4.16TEE255 pKa = 4.31INSDD259 pKa = 3.51EE260 pKa = 4.17AVAAGPDD267 pKa = 3.82GANDD271 pKa = 3.51SADD274 pKa = 4.18AEE276 pKa = 4.68DD277 pKa = 5.99EE278 pKa = 4.56PDD280 pKa = 3.8TDD282 pKa = 5.94DD283 pKa = 6.05NDD285 pKa = 4.17PADD288 pKa = 4.4NGNATATAPNTDD300 pKa = 3.03HH301 pKa = 7.06FVWLANRR308 pKa = 11.84NDD310 pKa = 3.59AQEE313 pKa = 4.52EE314 pKa = 4.37NTSCPACDD322 pKa = 3.34TAFSASDD329 pKa = 3.27VVIYY333 pKa = 8.65LNCGHH338 pKa = 6.31SWCSEE343 pKa = 3.94CVNLNYY349 pKa = 10.33RR350 pKa = 11.84SALRR354 pKa = 11.84SRR356 pKa = 11.84SSWPPQCCRR365 pKa = 11.84IDD367 pKa = 3.53IDD369 pKa = 3.92HH370 pKa = 7.01GSIAHH375 pKa = 6.73LLEE378 pKa = 5.17EE379 pKa = 4.91DD380 pKa = 3.63LQMDD384 pKa = 4.41LVVKK388 pKa = 10.58IEE390 pKa = 4.05EE391 pKa = 4.24FADD394 pKa = 3.63NNPVYY399 pKa = 10.61CQTPKK404 pKa = 10.63CAGGYY409 pKa = 9.35IPVEE413 pKa = 3.82RR414 pKa = 11.84RR415 pKa = 11.84EE416 pKa = 4.27GQWAGCQTCRR426 pKa = 11.84LTTCVEE432 pKa = 4.44CKK434 pKa = 10.25APAMAHH440 pKa = 6.3PMPALHH446 pKa = 7.08PNMIEE451 pKa = 4.14QVDD454 pKa = 3.77KK455 pKa = 11.17EE456 pKa = 4.13LAEE459 pKa = 4.3GQGWKK464 pKa = 9.37QCPGCRR470 pKa = 11.84NLVEE474 pKa = 4.54RR475 pKa = 11.84SGGCNYY481 pKa = 9.16MSCEE485 pKa = 4.37CGHH488 pKa = 6.45HH489 pKa = 6.52FCYY492 pKa = 10.28QCGGTLEE499 pKa = 4.96NNMPCDD505 pKa = 4.93CEE507 pKa = 4.91GQQPWVAQMNQEE519 pKa = 4.08AAGGADD525 pKa = 3.13QDD527 pKa = 4.05GDD529 pKa = 4.09SEE531 pKa = 4.78DD532 pKa = 4.13EE533 pKa = 4.09EE534 pKa = 6.58GEE536 pKa = 4.21DD537 pKa = 4.65GEE539 pKa = 5.08RR540 pKa = 11.84DD541 pKa = 3.56EE542 pKa = 6.14NEE544 pKa = 4.92DD545 pKa = 3.43GTEE548 pKa = 4.71DD549 pKa = 3.98EE550 pKa = 6.16AGDD553 pKa = 3.66QDD555 pKa = 4.88GDD557 pKa = 3.94EE558 pKa = 4.26QVQPAVQDD566 pKa = 3.62GGATGGWW573 pKa = 3.38

Molecular weight: 60.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2RJT9|W2RJT9_9EURO Uncharacterized protein OS=Cyphellophora europaea CBS 101466 OX=1220924 GN=HMPREF1541_09030 PE=4 SV=1
MM1 pKa = 7.41FGTRR5 pKa = 11.84RR6 pKa = 11.84SPRR9 pKa = 11.84THH11 pKa = 6.84AAPRR15 pKa = 11.84STRR18 pKa = 11.84HH19 pKa = 4.5TTTTTKK25 pKa = 10.14PGIMSRR31 pKa = 11.84LTGGGRR37 pKa = 11.84TRR39 pKa = 11.84KK40 pKa = 7.3THH42 pKa = 4.68TTTTTTAPRR51 pKa = 11.84TQRR54 pKa = 11.84KK55 pKa = 9.24SGWGTRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84APRR66 pKa = 11.84AAPVATHH73 pKa = 6.27HH74 pKa = 6.29RR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.93PSMGDD82 pKa = 3.11KK83 pKa = 10.62VAGALKK89 pKa = 10.14KK90 pKa = 10.61VQGSLTRR97 pKa = 11.84NPAKK101 pKa = 10.27KK102 pKa = 10.0AAGTRR107 pKa = 11.84RR108 pKa = 11.84MHH110 pKa = 5.68GTDD113 pKa = 2.87GRR115 pKa = 11.84GSRR118 pKa = 11.84RR119 pKa = 11.84VYY121 pKa = 10.87

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11090

0

11090

5402066

30

6474

487.1

53.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.093 ± 0.02

1.153 ± 0.008

5.775 ± 0.017

6.064 ± 0.018

3.616 ± 0.012

7.07 ± 0.019

2.402 ± 0.01

4.641 ± 0.014

4.677 ± 0.02

8.863 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.007

3.544 ± 0.012

6.041 ± 0.02

4.164 ± 0.017

6.18 ± 0.018

8.073 ± 0.023

6.002 ± 0.016

6.205 ± 0.016

1.497 ± 0.009

2.73 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski