Wenzhou crab virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH85|A0A1L3KH85_9VIRU Chropara_Vmeth domain-containing protein OS=Wenzhou crab virus 4 OX=1923561 PE=4 SV=1
MM1 pKa = 7.52AGIEE5 pKa = 4.12KK6 pKa = 10.18RR7 pKa = 11.84VLAVVPKK14 pKa = 8.86PHH16 pKa = 7.59PDD18 pKa = 3.91LIRR21 pKa = 11.84GRR23 pKa = 11.84TEE25 pKa = 3.62LPRR28 pKa = 11.84TFHH31 pKa = 6.11TAASALCPTRR41 pKa = 11.84TPATADD47 pKa = 3.04TAAPYY52 pKa = 10.52LGIPPIYY59 pKa = 9.85LQPLQQTSRR68 pKa = 11.84EE69 pKa = 4.11WQEE72 pKa = 3.55AVLDD76 pKa = 3.41IARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.61VQDD85 pKa = 3.13PTLPWSVVPTLEE97 pKa = 3.95EE98 pKa = 4.66LGDD101 pKa = 4.03SSCCHH106 pKa = 7.32LGPIPDD112 pKa = 3.94RR113 pKa = 11.84RR114 pKa = 11.84DD115 pKa = 3.38KK116 pKa = 10.48LTHH119 pKa = 6.32PWFKK123 pKa = 11.29SNLHH127 pKa = 5.95RR128 pKa = 11.84LVSEE132 pKa = 4.69FWKK135 pKa = 10.78LPEE138 pKa = 4.25NQWLDD143 pKa = 3.46AADD146 pKa = 4.15PLPFEE151 pKa = 4.28EE152 pKa = 4.53WVLRR156 pKa = 11.84FPLARR161 pKa = 11.84RR162 pKa = 11.84PLLRR166 pKa = 11.84QARR169 pKa = 11.84EE170 pKa = 3.82EE171 pKa = 4.13VHH173 pKa = 5.82QHH175 pKa = 5.49GLTDD179 pKa = 3.68KK180 pKa = 10.24DD181 pKa = 2.92AVMAVFIKK189 pKa = 10.19TEE191 pKa = 3.96TSTTATDD198 pKa = 3.68PRR200 pKa = 11.84NISPRR205 pKa = 11.84QLKK208 pKa = 9.59FLSVLGPYY216 pKa = 8.94VAAIEE221 pKa = 4.27KK222 pKa = 9.03QAKK225 pKa = 7.95RR226 pKa = 11.84CSYY229 pKa = 9.9LVKK232 pKa = 10.68GLVPEE237 pKa = 4.11QRR239 pKa = 11.84GKK241 pKa = 9.36RR242 pKa = 11.84TAQSWRR248 pKa = 11.84AEE250 pKa = 4.16VIEE253 pKa = 4.26TDD255 pKa = 4.29FSRR258 pKa = 11.84FDD260 pKa = 3.38MTVSQDD266 pKa = 3.37FIEE269 pKa = 4.28IVEE272 pKa = 3.89RR273 pKa = 11.84SMFRR277 pKa = 11.84RR278 pKa = 11.84AYY280 pKa = 9.4PAGSHH285 pKa = 7.1PDD287 pKa = 3.46FDD289 pKa = 4.59SCLQMLSRR297 pKa = 11.84MTGASSLGVLYY308 pKa = 10.89SVAGTRR314 pKa = 11.84ASGDD318 pKa = 3.08AHH320 pKa = 5.82TSIANGVLNRR330 pKa = 11.84FVIWACLRR338 pKa = 11.84NLDD341 pKa = 4.1PSTWDD346 pKa = 3.46SFHH349 pKa = 7.08EE350 pKa = 4.64GDD352 pKa = 5.63DD353 pKa = 5.25GIIHH357 pKa = 7.13CDD359 pKa = 3.3KK360 pKa = 10.56PVKK363 pKa = 10.17QDD365 pKa = 2.56ICDD368 pKa = 3.89YY369 pKa = 11.32LNFAALLGFKK379 pKa = 10.39LKK381 pKa = 10.3VVRR384 pKa = 11.84PLSHH388 pKa = 6.14EE389 pKa = 3.84HH390 pKa = 6.84ANFCGRR396 pKa = 11.84HH397 pKa = 4.39TCSVCHH403 pKa = 7.07KK404 pKa = 8.2EE405 pKa = 4.31FCDD408 pKa = 4.99LPRR411 pKa = 11.84TLSKK415 pKa = 10.36FHH417 pKa = 5.68VTFKK421 pKa = 10.58QGEE424 pKa = 4.33PKK426 pKa = 10.52SLLLAKK432 pKa = 10.02ALSYY436 pKa = 9.96MATDD440 pKa = 2.98NHH442 pKa = 6.05TPIISVLCQALIEE455 pKa = 4.38HH456 pKa = 7.3LSPVVSKK463 pKa = 10.87RR464 pKa = 11.84LLARR468 pKa = 11.84RR469 pKa = 11.84IAALGWWDD477 pKa = 3.77RR478 pKa = 11.84EE479 pKa = 3.99RR480 pKa = 11.84VKK482 pKa = 10.9QGMVTTQLEE491 pKa = 4.2PTACCRR497 pKa = 11.84AAVCSVYY504 pKa = 10.65DD505 pKa = 3.82WEE507 pKa = 5.25PALQLAWEE515 pKa = 4.32RR516 pKa = 11.84QLATWKK522 pKa = 11.08YY523 pKa = 9.42GVTWLQPLSVDD534 pKa = 4.49DD535 pKa = 4.36YY536 pKa = 11.65LVDD539 pKa = 3.75SDD541 pKa = 5.65AVTIYY546 pKa = 10.5QGG548 pKa = 3.06

Molecular weight: 61.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH85|A0A1L3KH85_9VIRU Chropara_Vmeth domain-containing protein OS=Wenzhou crab virus 4 OX=1923561 PE=4 SV=1
MM1 pKa = 6.92FTLLSTLTALLVGAAVDD18 pKa = 3.51RR19 pKa = 11.84AVYY22 pKa = 10.03PDD24 pKa = 3.26VHH26 pKa = 6.57EE27 pKa = 4.59PSLYY31 pKa = 10.45EE32 pKa = 3.92RR33 pKa = 11.84TGLPLFRR40 pKa = 11.84TLQQYY45 pKa = 10.05HH46 pKa = 5.97VLDD49 pKa = 3.79VLDD52 pKa = 5.1GVGLLNILGIPDD64 pKa = 3.83DD65 pKa = 3.92FQYY68 pKa = 11.18QEE70 pKa = 4.82LAHH73 pKa = 6.2SCQTRR78 pKa = 11.84YY79 pKa = 11.22DD80 pKa = 3.66NDD82 pKa = 3.16ATTLYY87 pKa = 10.18LRR89 pKa = 11.84SWEE92 pKa = 4.27LPHH95 pKa = 7.2LSRR98 pKa = 11.84SPLPWVCQRR107 pKa = 11.84LMSRR111 pKa = 11.84GHH113 pKa = 4.92VSEE116 pKa = 4.86GRR118 pKa = 11.84VSPLILALTAPLRR131 pKa = 11.84LAHH134 pKa = 5.78WTPSIVAFSVATIGLISLCAVAALVCFIRR163 pKa = 11.84RR164 pKa = 11.84YY165 pKa = 9.87RR166 pKa = 11.84LVDD169 pKa = 3.47QVCDD173 pKa = 3.6RR174 pKa = 11.84PPFSHH179 pKa = 7.19RR180 pKa = 11.84EE181 pKa = 3.83VRR183 pKa = 11.84TLLQRR188 pKa = 11.84QIDD191 pKa = 3.94RR192 pKa = 11.84RR193 pKa = 11.84VKK195 pKa = 10.23VSDD198 pKa = 3.38VTTTPTGHH206 pKa = 6.32EE207 pKa = 3.91RR208 pKa = 11.84LAHH211 pKa = 5.62IRR213 pKa = 11.84RR214 pKa = 11.84EE215 pKa = 4.13VEE217 pKa = 4.24GACLSVLSSISSKK230 pKa = 10.69LRR232 pKa = 11.84DD233 pKa = 3.43VGGSLSRR240 pKa = 11.84NARR243 pKa = 11.84LGKK246 pKa = 9.65NLHH249 pKa = 6.1ICFPSVDD256 pKa = 3.87ALDD259 pKa = 4.34KK260 pKa = 10.91LRR262 pKa = 11.84KK263 pKa = 8.11ATNPTPDD270 pKa = 3.48NDD272 pKa = 3.9VGHH275 pKa = 6.92HH276 pKa = 6.38RR277 pKa = 11.84GEE279 pKa = 4.69DD280 pKa = 3.77CPHH283 pKa = 6.69SNRR286 pKa = 11.84PTIMTYY292 pKa = 10.76VDD294 pKa = 3.69FHH296 pKa = 8.17LPIEE300 pKa = 4.25ALTRR304 pKa = 11.84CIRR307 pKa = 11.84SPTFIVTHH315 pKa = 6.53DD316 pKa = 4.07FARR319 pKa = 11.84VLGEE323 pKa = 3.87EE324 pKa = 4.04SWFDD328 pKa = 3.73GEE330 pKa = 4.36ATVNRR335 pKa = 11.84IGDD338 pKa = 4.93FISMTTRR345 pKa = 11.84GGSSYY350 pKa = 10.33VHH352 pKa = 6.81GFHH355 pKa = 6.49SWEE358 pKa = 4.12AEE360 pKa = 3.9GVVATEE366 pKa = 4.58HH367 pKa = 6.92GVFSYY372 pKa = 10.78QRR374 pKa = 11.84VYY376 pKa = 11.13DD377 pKa = 3.66EE378 pKa = 5.38HH379 pKa = 5.77NTIVLWCVPLPGRR392 pKa = 11.84FNPGRR397 pKa = 11.84DD398 pKa = 3.41NMLTSSAGRR407 pKa = 11.84VTDD410 pKa = 4.63FDD412 pKa = 4.32LADD415 pKa = 4.06GSRR418 pKa = 11.84VTLDD422 pKa = 3.13GEE424 pKa = 4.39QYY426 pKa = 10.58RR427 pKa = 11.84IARR430 pKa = 11.84PGAPVRR436 pKa = 11.84HH437 pKa = 5.66VPAKK441 pKa = 9.64TILRR445 pKa = 11.84VAFQMCIMARR455 pKa = 11.84DD456 pKa = 4.14EE457 pKa = 4.01KK458 pKa = 10.47WLPNLHH464 pKa = 5.62GVLRR468 pKa = 11.84GRR470 pKa = 11.84FAADD474 pKa = 3.27KK475 pKa = 11.14VNEE478 pKa = 4.15EE479 pKa = 4.58CLLPAQQLCVEE490 pKa = 5.02LSDD493 pKa = 5.18LMAMCYY499 pKa = 9.57RR500 pKa = 11.84RR501 pKa = 11.84SAIGDD506 pKa = 3.68PDD508 pKa = 3.83SYY510 pKa = 11.81GPLARR515 pKa = 11.84PIVRR519 pKa = 11.84MYY521 pKa = 11.13LRR523 pKa = 11.84VLSCLPYY530 pKa = 9.82TAEE533 pKa = 4.02QFCTRR538 pKa = 11.84LVLNFLAMLHH548 pKa = 6.26KK549 pKa = 10.36PGPTLPTSWAWNTVKK564 pKa = 10.48VPTYY568 pKa = 9.52EE569 pKa = 4.14VHH571 pKa = 6.37WDD573 pKa = 3.54QVASKK578 pKa = 10.73LVDD581 pKa = 3.42AQNGLRR587 pKa = 11.84VKK589 pKa = 10.65QPFRR593 pKa = 11.84NSGAVAGAPAPNQPCAPPRR612 pKa = 11.84PDD614 pKa = 3.09QRR616 pKa = 11.84KK617 pKa = 9.45SNGRR621 pKa = 11.84DD622 pKa = 2.98RR623 pKa = 11.84KK624 pKa = 9.39EE625 pKa = 4.12GPGGGSKK632 pKa = 7.14TTSGPHH638 pKa = 4.63QGKK641 pKa = 9.82DD642 pKa = 2.62RR643 pKa = 11.84VAQNVSHH650 pKa = 6.81GSKK653 pKa = 10.56CPVSNTYY660 pKa = 9.69PRR662 pKa = 11.84NCRR665 pKa = 11.84HH666 pKa = 5.86CRR668 pKa = 11.84AVSRR672 pKa = 11.84DD673 pKa = 3.71TAHH676 pKa = 7.45LPATTPANQPRR687 pKa = 11.84VAGGSAGHH695 pKa = 6.86RR696 pKa = 11.84PQAQGSRR703 pKa = 11.84PNSTVVSRR711 pKa = 11.84PHH713 pKa = 5.5FRR715 pKa = 11.84GARR718 pKa = 11.84RR719 pKa = 11.84QLVLPSRR726 pKa = 11.84PNPGQEE732 pKa = 3.8RR733 pKa = 11.84QIDD736 pKa = 3.73TSLVQIKK743 pKa = 7.76PTSSRR748 pKa = 11.84LRR750 pKa = 11.84VLEE753 pKa = 4.16APRR756 pKa = 11.84KK757 pKa = 8.06PVAGRR762 pKa = 11.84RR763 pKa = 11.84GSSSFF768 pKa = 3.39

Molecular weight: 85.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1675

359

768

558.3

62.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.239 ± 0.417

2.328 ± 0.562

5.612 ± 0.23

4.06 ± 0.42

3.403 ± 0.264

6.09 ± 0.66

2.985 ± 0.842

3.642 ± 0.222

3.642 ± 0.702

9.493 ± 0.959

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.552 ± 0.152

3.104 ± 0.778

7.284 ± 0.231

4.418 ± 0.672

8.358 ± 1.01

6.985 ± 0.348

6.627 ± 0.565

8.179 ± 0.472

1.731 ± 0.395

2.269 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski