[Eubacterium] cellulosolvens 6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; [Eubacterium] cellulosolvens

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2716 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I5AQ40|I5AQ40_EUBCE Uncharacterized protein OS=[Eubacterium] cellulosolvens 6 OX=633697 GN=EubceDRAFT1_0042 PE=4 SV=1
MM1 pKa = 7.67GLTGCGSYY9 pKa = 10.8VSPGKK14 pKa = 10.25NSEE17 pKa = 4.21YY18 pKa = 10.62LDD20 pKa = 4.02SSDD23 pKa = 5.56SFISTCAIMDD33 pKa = 4.15PGMIHH38 pKa = 7.09VIDD41 pKa = 5.43IIQNDD46 pKa = 3.82GMDD49 pKa = 3.76SLIKK53 pKa = 10.65FGTDD57 pKa = 2.68EE58 pKa = 5.28DD59 pKa = 4.6GNEE62 pKa = 4.06LSAQAIMTKK71 pKa = 9.54IYY73 pKa = 10.57AKK75 pKa = 10.2QSEE78 pKa = 4.66LEE80 pKa = 3.98QPVIDD85 pKa = 4.31DD86 pKa = 4.92CEE88 pKa = 4.58ALLYY92 pKa = 10.36GSPDD96 pKa = 2.84HH97 pKa = 6.98FMDD100 pKa = 5.68VITKK104 pKa = 10.43NDD106 pKa = 3.47GKK108 pKa = 11.0LDD110 pKa = 3.93NDD112 pKa = 4.08DD113 pKa = 4.18VEE115 pKa = 5.13HH116 pKa = 6.91LLEE119 pKa = 5.54AIGEE123 pKa = 4.12IQAAGNMVGVQDD135 pKa = 5.61IPTQIQNAAINFDD148 pKa = 4.01NNGPDD153 pKa = 3.33ANN155 pKa = 4.09

Molecular weight:
16.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I5AQD0|I5AQD0_EUBCE Metal-dependent hydrolase beta-lactamase superfamily I OS=[Eubacterium] cellulosolvens 6 OX=633697 GN=EubceDRAFT1_0141 PE=4 SV=1
MM1 pKa = 7.69AEE3 pKa = 4.13VEE5 pKa = 4.26NTTAATAEE13 pKa = 4.19NNEE16 pKa = 4.17RR17 pKa = 11.84PAKK20 pKa = 9.94ADD22 pKa = 3.57RR23 pKa = 11.84AAAPARR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84VTRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.14VCVFCGKK45 pKa = 10.29DD46 pKa = 3.19NEE48 pKa = 4.48IDD50 pKa = 3.7YY51 pKa = 11.3KK52 pKa = 11.11DD53 pKa = 3.32AAKK56 pKa = 10.17LRR58 pKa = 11.84RR59 pKa = 11.84YY60 pKa = 9.43ISEE63 pKa = 3.95RR64 pKa = 11.84GKK66 pKa = 9.88ILPRR70 pKa = 11.84RR71 pKa = 11.84VTGNCAKK78 pKa = 9.83HH79 pKa = 5.26QRR81 pKa = 11.84AITLAVKK88 pKa = 10.39RR89 pKa = 11.84ARR91 pKa = 11.84HH92 pKa = 5.33LAILPYY98 pKa = 10.59VEE100 pKa = 5.17DD101 pKa = 3.58

Molecular weight:
11.52 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2716

0

2716

943410

30

4372

347.4

38.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.783 ± 0.043

1.395 ± 0.019

6.218 ± 0.046

7.615 ± 0.055

3.939 ± 0.03

7.313 ± 0.047

1.763 ± 0.02

6.761 ± 0.046

6.526 ± 0.039

8.534 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.981 ± 0.027

4.268 ± 0.031

3.375 ± 0.028

3.017 ± 0.024

5.022 ± 0.046

5.986 ± 0.036

5.589 ± 0.054

6.924 ± 0.04

0.924 ± 0.017

4.067 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski