[Eubacterium] cellulosolvens 6
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2716 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I5AQ40|I5AQ40_EUBCE Uncharacterized protein OS=[Eubacterium] cellulosolvens 6 OX=633697 GN=EubceDRAFT1_0042 PE=4 SV=1
MM1 pKa = 7.67 GLTGCGSYY9 pKa = 10.8 VSPGKK14 pKa = 10.25 NSEE17 pKa = 4.21 YY18 pKa = 10.62 LDD20 pKa = 4.02 SSDD23 pKa = 5.56 SFISTCAIMDD33 pKa = 4.15 PGMIHH38 pKa = 7.09 VIDD41 pKa = 5.43 IIQNDD46 pKa = 3.82 GMDD49 pKa = 3.76 SLIKK53 pKa = 10.65 FGTDD57 pKa = 2.68 EE58 pKa = 5.28 DD59 pKa = 4.6 GNEE62 pKa = 4.06 LSAQAIMTKK71 pKa = 9.54 IYY73 pKa = 10.57 AKK75 pKa = 10.2 QSEE78 pKa = 4.66 LEE80 pKa = 3.98 QPVIDD85 pKa = 4.31 DD86 pKa = 4.92 CEE88 pKa = 4.58 ALLYY92 pKa = 10.36 GSPDD96 pKa = 2.84 HH97 pKa = 6.98 FMDD100 pKa = 5.68 VITKK104 pKa = 10.43 NDD106 pKa = 3.47 GKK108 pKa = 11.0 LDD110 pKa = 3.93 NDD112 pKa = 4.08 DD113 pKa = 4.18 VEE115 pKa = 5.13 HH116 pKa = 6.91 LLEE119 pKa = 5.54 AIGEE123 pKa = 4.12 IQAAGNMVGVQDD135 pKa = 5.61 IPTQIQNAAINFDD148 pKa = 4.01 NNGPDD153 pKa = 3.33 ANN155 pKa = 4.09
Molecular weight: 16.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.694
IPC_protein 3.694
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|I5AQD0|I5AQD0_EUBCE Metal-dependent hydrolase beta-lactamase superfamily I OS=[Eubacterium] cellulosolvens 6 OX=633697 GN=EubceDRAFT1_0141 PE=4 SV=1
MM1 pKa = 7.69 AEE3 pKa = 4.13 VEE5 pKa = 4.26 NTTAATAEE13 pKa = 4.19 NNEE16 pKa = 4.17 RR17 pKa = 11.84 PAKK20 pKa = 9.94 ADD22 pKa = 3.57 RR23 pKa = 11.84 AAAPARR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 VTRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.14 VCVFCGKK45 pKa = 10.29 DD46 pKa = 3.19 NEE48 pKa = 4.48 IDD50 pKa = 3.7 YY51 pKa = 11.3 KK52 pKa = 11.11 DD53 pKa = 3.32 AAKK56 pKa = 10.17 LRR58 pKa = 11.84 RR59 pKa = 11.84 YY60 pKa = 9.43 ISEE63 pKa = 3.95 RR64 pKa = 11.84 GKK66 pKa = 9.88 ILPRR70 pKa = 11.84 RR71 pKa = 11.84 VTGNCAKK78 pKa = 9.83 HH79 pKa = 5.26 QRR81 pKa = 11.84 AITLAVKK88 pKa = 10.39 RR89 pKa = 11.84 ARR91 pKa = 11.84 HH92 pKa = 5.33 LAILPYY98 pKa = 10.59 VEE100 pKa = 5.17 DD101 pKa = 3.58
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.619
IPC_protein 10.409
Toseland 10.745
ProMoST 10.438
Dawson 10.818
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.023
Grimsley 10.862
Solomon 10.965
Lehninger 10.921
Nozaki 10.745
DTASelect 10.526
Thurlkill 10.73
EMBOSS 11.155
Sillero 10.76
Patrickios 10.76
IPC_peptide 10.965
IPC2_peptide 9.633
IPC2.peptide.svr19 8.654
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2716
0
2716
943410
30
4372
347.4
38.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.783 ± 0.043
1.395 ± 0.019
6.218 ± 0.046
7.615 ± 0.055
3.939 ± 0.03
7.313 ± 0.047
1.763 ± 0.02
6.761 ± 0.046
6.526 ± 0.039
8.534 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.981 ± 0.027
4.268 ± 0.031
3.375 ± 0.028
3.017 ± 0.024
5.022 ± 0.046
5.986 ± 0.036
5.589 ± 0.054
6.924 ± 0.04
0.924 ± 0.017
4.067 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here