Cosavirus A (isolate Human/Pakistan/0553/-) (HCoSV-A)
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|B8XTP8|POLG_COSAA Genome polyprotein OS=Cosavirus A (isolate Human/Pakistan/0553/-) OX=1554483 PE=3 SV=1
MM1 pKa = 7.44 GANNSKK7 pKa = 10.69 EE8 pKa = 4.34 SVSSNGNEE16 pKa = 3.66 GTIVNNFYY24 pKa = 11.14 SNQYY28 pKa = 8.31 YY29 pKa = 11.04 ASIDD33 pKa = 3.3 ASAQGVGTSTTPEE46 pKa = 3.87 NGNVSGFLGLAGSAFNALSLLASPRR71 pKa = 11.84 TEE73 pKa = 3.63 TGMMMEE79 pKa = 4.75 DD80 pKa = 2.88 RR81 pKa = 11.84 VLSRR85 pKa = 11.84 TAGNTSVNSQAAEE98 pKa = 4.24 GVLQAYY104 pKa = 7.31 GTEE107 pKa = 4.25 TDD109 pKa = 3.84 SNSPTSCGDD118 pKa = 3.57 DD119 pKa = 3.6 PSKK122 pKa = 10.45 GTHH125 pKa = 4.9 ATDD128 pKa = 3.13 RR129 pKa = 11.84 AFVIQLLPWKK139 pKa = 8.73 QTTNSYY145 pKa = 8.9 FAQWVRR151 pKa = 11.84 LTQKK155 pKa = 10.66 LSNNLHH161 pKa = 5.91 GNVMAKK167 pKa = 10.11 NIKK170 pKa = 9.11 SHH172 pKa = 6.68 AFAKK176 pKa = 10.1 MGFEE180 pKa = 5.1 VMLQANTSPFHH191 pKa = 6.57 NGILGLFLVPEE202 pKa = 4.83 FVRR205 pKa = 11.84 KK206 pKa = 10.36 GEE208 pKa = 4.24 ITDD211 pKa = 3.23 EE212 pKa = 4.61 WIDD215 pKa = 3.78 LTPTSSLVSNTEE227 pKa = 3.76 LYY229 pKa = 10.78 NPQTYY234 pKa = 11.29 ANFPFDD240 pKa = 3.86 AKK242 pKa = 10.88 HH243 pKa = 6.23 SFDD246 pKa = 4.11 YY247 pKa = 11.37 SDD249 pKa = 3.24 ITPEE253 pKa = 3.49 QFMIFPHH260 pKa = 5.9 QLINPKK266 pKa = 8.59 DD267 pKa = 3.78 TNVATVRR274 pKa = 11.84 VPYY277 pKa = 10.58 INIAPTNDD285 pKa = 3.49 TTVHH289 pKa = 5.1 TVWTAVVMVLVPLNFSSGASPTVSLTLTITPINSVFNGLHH329 pKa = 5.86 HH330 pKa = 6.79 TAQGPIPVRR339 pKa = 11.84 PFHH342 pKa = 6.67 NFQQFSTTVPLRR354 pKa = 11.84 TEE356 pKa = 3.73 PCYY359 pKa = 11.16 GMTVTPPVDD368 pKa = 3.94 YY369 pKa = 9.95 MPLPITDD376 pKa = 4.12 LVEE379 pKa = 4.06 LAKK382 pKa = 10.79 VPSFVTVANSDD393 pKa = 3.71 TTSEE397 pKa = 3.82 RR398 pKa = 11.84 SFPYY402 pKa = 9.94 FSVSNTEE409 pKa = 3.54 QGRR412 pKa = 11.84 NLFKK416 pKa = 11.01 SSVVLSDD423 pKa = 3.46 LHH425 pKa = 5.74 YY426 pKa = 10.95 QHH428 pKa = 6.8 TLVANLARR436 pKa = 11.84 YY437 pKa = 7.6 FCNYY441 pKa = 9.54 RR442 pKa = 11.84 GSLQFDD448 pKa = 4.67 FIAATTAMTRR458 pKa = 11.84 GKK460 pKa = 10.5 LLISYY465 pKa = 7.5 TPPGAGEE472 pKa = 4.02 PQSIDD477 pKa = 3.18 QAMMGTYY484 pKa = 10.25 AIWDD488 pKa = 4.09 LGLQSTFNFVVPFISASDD506 pKa = 3.61 FRR508 pKa = 11.84 FNTSSVSNALNSDD521 pKa = 2.97 GWITVWLMNPLTYY534 pKa = 10.18 PPSTPPTQQILMLMSAGSDD553 pKa = 3.22 FSYY556 pKa = 10.79 RR557 pKa = 11.84 LPISPGFAEE566 pKa = 5.23 GEE568 pKa = 4.38 TSEE571 pKa = 5.48 HH572 pKa = 6.27 PMDD575 pKa = 3.93 NAEE578 pKa = 4.83 CGKK581 pKa = 10.05 IDD583 pKa = 5.15 DD584 pKa = 4.9 KK585 pKa = 11.5 DD586 pKa = 3.69 AGMFSGHH593 pKa = 5.75 SVGLPTPHH601 pKa = 6.9 TSTSFFYY608 pKa = 10.74 DD609 pKa = 3.36 RR610 pKa = 11.84 YY611 pKa = 9.76 RR612 pKa = 11.84 FVGIVKK618 pKa = 10.1 SVVNNTPKK626 pKa = 10.36 PVNIYY631 pKa = 11.04 DD632 pKa = 3.67 DD633 pKa = 3.59 TGKK636 pKa = 10.62 VKK638 pKa = 10.49 NLQQVFPTSDD648 pKa = 2.89 TLLPHH653 pKa = 6.77 SLMSLSPCASVCGQPISSFLFAQRR677 pKa = 11.84 ANPKK681 pKa = 8.58 KK682 pKa = 8.68 TLKK685 pKa = 10.38 LRR687 pKa = 11.84 SGDD690 pKa = 3.4 EE691 pKa = 3.79 FLYY694 pKa = 10.28 RR695 pKa = 11.84 CCPFSYY701 pKa = 10.0 IKK703 pKa = 10.45 CDD705 pKa = 3.46 LEE707 pKa = 4.38 FTVVPPANSTRR718 pKa = 11.84 DD719 pKa = 4.15 YY720 pKa = 10.49 IVHH723 pKa = 7.25 WYY725 pKa = 9.12 PPGATLDD732 pKa = 3.7 AGEE735 pKa = 4.29 VAVGNTSGSNGFDD748 pKa = 3.7 DD749 pKa = 4.75 NGMNAGSSLFSYY761 pKa = 10.83 NPTFHH766 pKa = 6.96 ARR768 pKa = 11.84 APSKK772 pKa = 10.63 VSAVIPFCLPVSLLPLYY789 pKa = 10.78 FDD791 pKa = 4.83 GFPDD795 pKa = 3.66 YY796 pKa = 10.84 STTKK800 pKa = 10.49 GMYY803 pKa = 8.88 GCSPSFSFGTIYY815 pKa = 10.27 IEE817 pKa = 4.68 SGLQEE822 pKa = 4.33 TYY824 pKa = 10.68 SVYY827 pKa = 10.31 IRR829 pKa = 11.84 YY830 pKa = 9.56 KK831 pKa = 10.37 DD832 pKa = 3.69 FKK834 pKa = 10.79 GYY836 pKa = 10.87 APRR839 pKa = 11.84 PLIRR843 pKa = 11.84 TPHH846 pKa = 5.5 IRR848 pKa = 11.84 LSEE851 pKa = 3.9 RR852 pKa = 11.84 ARR854 pKa = 11.84 YY855 pKa = 9.48 IMADD859 pKa = 3.2 SVLPRR864 pKa = 11.84 PLTRR868 pKa = 11.84 AEE870 pKa = 3.73 RR871 pKa = 11.84 DD872 pKa = 3.47 VARR875 pKa = 11.84 DD876 pKa = 3.43 LLLIAGDD883 pKa = 3.76 IEE885 pKa = 5.27 SNPGPAFNPEE895 pKa = 4.01 YY896 pKa = 9.13 TAHH899 pKa = 6.57 GPVTEE904 pKa = 5.45 LIQLARR910 pKa = 11.84 KK911 pKa = 9.31 PEE913 pKa = 4.09 TVDD916 pKa = 3.04 NVNRR920 pKa = 11.84 LLTTLNTLMAKK931 pKa = 9.25 WNNLKK936 pKa = 9.28 DD937 pKa = 3.81 TVTDD941 pKa = 3.8 AVFLRR946 pKa = 11.84 DD947 pKa = 4.01 MVCLLVKK954 pKa = 9.69 LTSLMYY960 pKa = 10.23 LVHH963 pKa = 6.11 GQGPGAYY970 pKa = 8.82 FAAASILLADD980 pKa = 5.8 GITFFDD986 pKa = 3.76 WYY988 pKa = 10.08 EE989 pKa = 3.97 KK990 pKa = 10.59 IKK992 pKa = 10.86 IFMARR997 pKa = 11.84 KK998 pKa = 9.43 LRR1000 pKa = 11.84 VSPPFFPAAQGPDD1013 pKa = 3.01 LRR1015 pKa = 11.84 DD1016 pKa = 3.1 FVTFFNAARR1025 pKa = 11.84 GAQWMIDD1032 pKa = 3.6 SLKK1035 pKa = 11.18 SLITCIKK1042 pKa = 9.21 QWLEE1046 pKa = 3.8 LEE1048 pKa = 4.4 EE1049 pKa = 4.2 EE1050 pKa = 4.33 NEE1052 pKa = 4.05 AVQLEE1057 pKa = 4.41 KK1058 pKa = 10.56 MLIDD1062 pKa = 4.15 SPRR1065 pKa = 11.84 HH1066 pKa = 5.01 CKK1068 pKa = 10.32 AINDD1072 pKa = 3.9 YY1073 pKa = 11.24 NRR1075 pKa = 11.84 GDD1077 pKa = 3.56 SFQRR1081 pKa = 11.84 PTNSFEE1087 pKa = 4.03 FMDD1090 pKa = 4.42 RR1091 pKa = 11.84 LVEE1094 pKa = 4.14 CATKK1098 pKa = 10.46 LGKK1101 pKa = 9.82 VQIATYY1107 pKa = 9.9 FRR1109 pKa = 11.84 NFTTADD1115 pKa = 3.42 SDD1117 pKa = 3.76 TSRR1120 pKa = 11.84 PEE1122 pKa = 3.56 PVVVVLRR1129 pKa = 11.84 GKK1131 pKa = 9.88 PGVGKK1136 pKa = 10.22 SAAATVMAAAVSKK1149 pKa = 10.64 LLVGSQSVYY1158 pKa = 10.15 TLSPDD1163 pKa = 3.6 TEE1165 pKa = 4.51 HH1166 pKa = 6.78 MDD1168 pKa = 3.77 GYY1170 pKa = 10.59 HH1171 pKa = 5.7 GQFVTLMDD1179 pKa = 5.37 DD1180 pKa = 4.56 LGQNPDD1186 pKa = 3.91 GEE1188 pKa = 4.55 DD1189 pKa = 3.66 FRR1191 pKa = 11.84 CFCQMVSCAQYY1202 pKa = 10.05 RR1203 pKa = 11.84 PAMADD1208 pKa = 3.39 LKK1210 pKa = 11.27 DD1211 pKa = 3.54 KK1212 pKa = 11.42 GILFTSRR1219 pKa = 11.84 LLIATTNLPDD1229 pKa = 4.43 FNPVTISDD1237 pKa = 3.62 PRR1239 pKa = 11.84 ALDD1242 pKa = 3.27 RR1243 pKa = 11.84 RR1244 pKa = 11.84 ITFDD1248 pKa = 3.01 ILVTPGSAATKK1259 pKa = 10.28 NGKK1262 pKa = 9.44 LDD1264 pKa = 3.43 LAAALKK1270 pKa = 10.15 PDD1272 pKa = 3.77 GPGEE1276 pKa = 4.0 HH1277 pKa = 7.72 PYY1279 pKa = 10.74 TSDD1282 pKa = 4.19 CPILHH1287 pKa = 5.53 TTGLLLKK1294 pKa = 10.27 NLRR1297 pKa = 11.84 NNQTMNLKK1305 pKa = 10.45 DD1306 pKa = 4.09 LVDD1309 pKa = 4.5 MIVKK1313 pKa = 10.32 RR1314 pKa = 11.84 IKK1316 pKa = 10.17 HH1317 pKa = 5.4 KK1318 pKa = 11.1 KK1319 pKa = 8.58 EE1320 pKa = 3.62 VGNMLDD1326 pKa = 3.38 SLVAQGPTMIVGYY1339 pKa = 8.73 TKK1341 pKa = 10.88 DD1342 pKa = 3.7 DD1343 pKa = 3.99 DD1344 pKa = 5.14 GIAIVDD1350 pKa = 5.04 CLEE1353 pKa = 3.81 EE1354 pKa = 4.0 WNKK1357 pKa = 10.37 IKK1359 pKa = 10.58 DD1360 pKa = 3.68 KK1361 pKa = 10.93 KK1362 pKa = 10.54 KK1363 pKa = 10.12 KK1364 pKa = 9.72 QLALEE1369 pKa = 4.3 MVAQEE1374 pKa = 4.87 LKK1376 pKa = 10.73 DD1377 pKa = 3.14 KK1378 pKa = 11.17 HH1379 pKa = 6.42 EE1380 pKa = 4.12 EE1381 pKa = 3.92 HH1382 pKa = 6.98 KK1383 pKa = 10.77 GTIKK1387 pKa = 10.3 LLKK1390 pKa = 9.96 MFVTGLGVVAAVAGAYY1406 pKa = 8.54 ATMKK1410 pKa = 10.53 YY1411 pKa = 7.49 FTKK1414 pKa = 10.49 DD1415 pKa = 2.9 KK1416 pKa = 10.42 PKK1418 pKa = 10.67 EE1419 pKa = 4.01 EE1420 pKa = 4.18 EE1421 pKa = 4.11 EE1422 pKa = 4.06 EE1423 pKa = 4.27 PEE1425 pKa = 3.95 EE1426 pKa = 4.18 KK1427 pKa = 10.23 KK1428 pKa = 10.62 EE1429 pKa = 4.15 KK1430 pKa = 9.4 KK1431 pKa = 9.08 TEE1433 pKa = 3.98 EE1434 pKa = 4.23 SKK1436 pKa = 10.93 EE1437 pKa = 3.66 AAGPYY1442 pKa = 9.7 NGPTKK1447 pKa = 10.73 KK1448 pKa = 10.18 EE1449 pKa = 3.75 IKK1451 pKa = 8.92 TLKK1454 pKa = 10.52 LKK1456 pKa = 10.54 AQSPLMDD1463 pKa = 3.75 MEE1465 pKa = 5.07 KK1466 pKa = 10.51 KK1467 pKa = 9.74 IAQNVMPFQIFYY1479 pKa = 10.15 NGKK1482 pKa = 10.16 RR1483 pKa = 11.84 YY1484 pKa = 7.02 TQSCLAIGKK1493 pKa = 9.4 RR1494 pKa = 11.84 VILVNKK1500 pKa = 9.56 HH1501 pKa = 5.21 AFEE1504 pKa = 4.28 SVEE1507 pKa = 4.01 HH1508 pKa = 6.54 KK1509 pKa = 10.72 FVVDD1513 pKa = 3.56 QKK1515 pKa = 11.3 EE1516 pKa = 4.1 YY1517 pKa = 10.4 TLDD1520 pKa = 3.27 QVTAISLDD1528 pKa = 3.8 CGSGVTDD1535 pKa = 3.4 VCAVCLPPGPDD1546 pKa = 3.05 FKK1548 pKa = 11.19 SIKK1551 pKa = 10.01 KK1552 pKa = 9.85 HH1553 pKa = 5.15 FLPFNTTMFPGTRR1566 pKa = 11.84 LTILSNDD1573 pKa = 3.97 HH1574 pKa = 6.4 YY1575 pKa = 10.84 PMSRR1579 pKa = 11.84 EE1580 pKa = 3.68 GSFLRR1585 pKa = 11.84 FEE1587 pKa = 5.19 DD1588 pKa = 4.27 EE1589 pKa = 4.2 VPTNVGNMPFVMLYY1603 pKa = 10.63 KK1604 pKa = 8.86 STSYY1608 pKa = 10.94 FGMCGSVVCSRR1619 pKa = 11.84 FVDD1622 pKa = 3.63 GGGIIGMHH1630 pKa = 6.46 CAGGGGVSVGTRR1642 pKa = 11.84 LTARR1646 pKa = 11.84 MIEE1649 pKa = 4.23 SVFDD1653 pKa = 3.36 YY1654 pKa = 9.25 FYY1656 pKa = 10.86 PPVAQGIIEE1665 pKa = 4.1 NTEE1668 pKa = 3.62 TGPRR1672 pKa = 11.84 VHH1674 pKa = 6.75 VPRR1677 pKa = 11.84 TSKK1680 pKa = 10.76 LKK1682 pKa = 9.56 RR1683 pKa = 11.84 TNATYY1688 pKa = 8.91 PATEE1692 pKa = 4.33 KK1693 pKa = 10.78 YY1694 pKa = 10.62 GPAALSRR1701 pKa = 11.84 YY1702 pKa = 9.53 DD1703 pKa = 3.27 PRR1705 pKa = 11.84 LNEE1708 pKa = 3.99 GVNLDD1713 pKa = 3.47 EE1714 pKa = 5.67 VIFSKK1719 pKa = 8.98 HH1720 pKa = 4.04 TQNTLVEE1727 pKa = 4.39 KK1728 pKa = 10.9 GSTFRR1733 pKa = 11.84 SALDD1737 pKa = 3.46 MAAEE1741 pKa = 4.29 IYY1743 pKa = 10.66 GEE1745 pKa = 4.14 KK1746 pKa = 10.52 FRR1748 pKa = 11.84 GNDD1751 pKa = 3.59 FSPLSVEE1758 pKa = 4.35 DD1759 pKa = 5.12 AILGIPGLDD1768 pKa = 2.74 RR1769 pKa = 11.84 LDD1771 pKa = 4.44 PNTASGLPYY1780 pKa = 10.17 TKK1782 pKa = 9.69 TRR1784 pKa = 11.84 RR1785 pKa = 11.84 QMIDD1789 pKa = 3.19 FNTGQILDD1797 pKa = 4.04 DD1798 pKa = 3.77 TLKK1801 pKa = 10.81 CRR1803 pKa = 11.84 LGQWLAGRR1811 pKa = 11.84 PPQEE1815 pKa = 3.37 VHH1817 pKa = 5.59 YY1818 pKa = 9.24 QTFLKK1823 pKa = 10.93 DD1824 pKa = 3.64 EE1825 pKa = 4.03 IRR1827 pKa = 11.84 PIEE1830 pKa = 4.16 KK1831 pKa = 10.27 VKK1833 pKa = 10.79 AGKK1836 pKa = 7.9 TRR1838 pKa = 11.84 IIDD1841 pKa = 3.9 VPPLDD1846 pKa = 3.69 HH1847 pKa = 7.18 VIAFRR1852 pKa = 11.84 MLFGRR1857 pKa = 11.84 FIAHH1861 pKa = 5.05 YY1862 pKa = 9.5 HH1863 pKa = 5.98 LNFGFKK1869 pKa = 9.07 TGSAIGCDD1877 pKa = 3.86 PDD1879 pKa = 3.85 VAWASFGFEE1888 pKa = 3.95 LSGFPYY1894 pKa = 10.39 LYY1896 pKa = 11.01 DD1897 pKa = 3.56 FDD1899 pKa = 4.97 YY1900 pKa = 11.67 SNFDD1904 pKa = 3.55 ASHH1907 pKa = 5.52 STSIFEE1913 pKa = 4.13 ILEE1916 pKa = 3.91 QKK1918 pKa = 10.39 FFSPEE1923 pKa = 3.61 LGFDD1927 pKa = 3.78 PRR1929 pKa = 11.84 CSLLLKK1935 pKa = 10.33 SLAVSTHH1942 pKa = 5.95 CYY1944 pKa = 8.41 EE1945 pKa = 4.26 NKK1947 pKa = 9.91 RR1948 pKa = 11.84 LQIAGGLPSGTAGTSVLNTVINNIIFHH1975 pKa = 6.35 GALYY1979 pKa = 8.48 HH1980 pKa = 6.61 TYY1982 pKa = 10.79 TNFEE1986 pKa = 4.04 RR1987 pKa = 11.84 DD1988 pKa = 4.55 DD1989 pKa = 4.13 ISMLAYY1995 pKa = 10.42 GDD1997 pKa = 4.92 DD1998 pKa = 3.15 IVVASKK2004 pKa = 11.22 FEE2006 pKa = 4.49 LDD2008 pKa = 3.44 LVMVKK2013 pKa = 10.9 AFMNRR2018 pKa = 11.84 IGYY2021 pKa = 9.68 KK2022 pKa = 8.63 ITPADD2027 pKa = 3.51 KK2028 pKa = 10.75 SDD2030 pKa = 3.46 EE2031 pKa = 4.03 FRR2033 pKa = 11.84 PKK2035 pKa = 10.81 CMDD2038 pKa = 5.12 DD2039 pKa = 2.85 ICFLKK2044 pKa = 10.63 RR2045 pKa = 11.84 RR2046 pKa = 11.84 FVKK2049 pKa = 10.62 VAGVWAPVMEE2059 pKa = 4.42 TEE2061 pKa = 4.36 NLEE2064 pKa = 5.53 AMLSWYY2070 pKa = 10.36 KK2071 pKa = 10.66 PGTLNEE2077 pKa = 4.2 KK2078 pKa = 9.44 LQSVSRR2084 pKa = 11.84 LAHH2087 pKa = 5.46 FSGRR2091 pKa = 11.84 DD2092 pKa = 3.57 VYY2094 pKa = 11.78 DD2095 pKa = 3.5 HH2096 pKa = 6.89 LFKK2099 pKa = 10.98 PFIRR2103 pKa = 11.84 DD2104 pKa = 3.53 GFDD2107 pKa = 3.03 VTPWKK2112 pKa = 10.04 QLHH2115 pKa = 6.23 LEE2117 pKa = 4.12 WLNKK2121 pKa = 10.04 LSAA2124 pKa = 4.12
Molecular weight: 235.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.953
IPC2_protein 5.944
IPC_protein 6.02
Toseland 6.351
ProMoST 6.415
Dawson 6.326
Bjellqvist 6.338
Wikipedia 6.351
Rodwell 6.313
Grimsley 6.491
Solomon 6.326
Lehninger 6.326
Nozaki 6.62
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.707
Patrickios 4.113
IPC_peptide 6.351
IPC2_peptide 6.62
IPC2.peptide.svr19 6.645
Protein with the highest isoelectric point:
>sp|B8XTP8|POLG_COSAA Genome polyprotein OS=Cosavirus A (isolate Human/Pakistan/0553/-) OX=1554483 PE=3 SV=1
MM1 pKa = 7.44 GANNSKK7 pKa = 10.69 EE8 pKa = 4.34 SVSSNGNEE16 pKa = 3.66 GTIVNNFYY24 pKa = 11.14 SNQYY28 pKa = 8.31 YY29 pKa = 11.04 ASIDD33 pKa = 3.3 ASAQGVGTSTTPEE46 pKa = 3.87 NGNVSGFLGLAGSAFNALSLLASPRR71 pKa = 11.84 TEE73 pKa = 3.63 TGMMMEE79 pKa = 4.75 DD80 pKa = 2.88 RR81 pKa = 11.84 VLSRR85 pKa = 11.84 TAGNTSVNSQAAEE98 pKa = 4.24 GVLQAYY104 pKa = 7.31 GTEE107 pKa = 4.25 TDD109 pKa = 3.84 SNSPTSCGDD118 pKa = 3.57 DD119 pKa = 3.6 PSKK122 pKa = 10.45 GTHH125 pKa = 4.9 ATDD128 pKa = 3.13 RR129 pKa = 11.84 AFVIQLLPWKK139 pKa = 8.73 QTTNSYY145 pKa = 8.9 FAQWVRR151 pKa = 11.84 LTQKK155 pKa = 10.66 LSNNLHH161 pKa = 5.91 GNVMAKK167 pKa = 10.11 NIKK170 pKa = 9.11 SHH172 pKa = 6.68 AFAKK176 pKa = 10.1 MGFEE180 pKa = 5.1 VMLQANTSPFHH191 pKa = 6.57 NGILGLFLVPEE202 pKa = 4.83 FVRR205 pKa = 11.84 KK206 pKa = 10.36 GEE208 pKa = 4.24 ITDD211 pKa = 3.23 EE212 pKa = 4.61 WIDD215 pKa = 3.78 LTPTSSLVSNTEE227 pKa = 3.76 LYY229 pKa = 10.78 NPQTYY234 pKa = 11.29 ANFPFDD240 pKa = 3.86 AKK242 pKa = 10.88 HH243 pKa = 6.23 SFDD246 pKa = 4.11 YY247 pKa = 11.37 SDD249 pKa = 3.24 ITPEE253 pKa = 3.49 QFMIFPHH260 pKa = 5.9 QLINPKK266 pKa = 8.59 DD267 pKa = 3.78 TNVATVRR274 pKa = 11.84 VPYY277 pKa = 10.58 INIAPTNDD285 pKa = 3.49 TTVHH289 pKa = 5.1 TVWTAVVMVLVPLNFSSGASPTVSLTLTITPINSVFNGLHH329 pKa = 5.86 HH330 pKa = 6.79 TAQGPIPVRR339 pKa = 11.84 PFHH342 pKa = 6.67 NFQQFSTTVPLRR354 pKa = 11.84 TEE356 pKa = 3.73 PCYY359 pKa = 11.16 GMTVTPPVDD368 pKa = 3.94 YY369 pKa = 9.95 MPLPITDD376 pKa = 4.12 LVEE379 pKa = 4.06 LAKK382 pKa = 10.79 VPSFVTVANSDD393 pKa = 3.71 TTSEE397 pKa = 3.82 RR398 pKa = 11.84 SFPYY402 pKa = 9.94 FSVSNTEE409 pKa = 3.54 QGRR412 pKa = 11.84 NLFKK416 pKa = 11.01 SSVVLSDD423 pKa = 3.46 LHH425 pKa = 5.74 YY426 pKa = 10.95 QHH428 pKa = 6.8 TLVANLARR436 pKa = 11.84 YY437 pKa = 7.6 FCNYY441 pKa = 9.54 RR442 pKa = 11.84 GSLQFDD448 pKa = 4.67 FIAATTAMTRR458 pKa = 11.84 GKK460 pKa = 10.5 LLISYY465 pKa = 7.5 TPPGAGEE472 pKa = 4.02 PQSIDD477 pKa = 3.18 QAMMGTYY484 pKa = 10.25 AIWDD488 pKa = 4.09 LGLQSTFNFVVPFISASDD506 pKa = 3.61 FRR508 pKa = 11.84 FNTSSVSNALNSDD521 pKa = 2.97 GWITVWLMNPLTYY534 pKa = 10.18 PPSTPPTQQILMLMSAGSDD553 pKa = 3.22 FSYY556 pKa = 10.79 RR557 pKa = 11.84 LPISPGFAEE566 pKa = 5.23 GEE568 pKa = 4.38 TSEE571 pKa = 5.48 HH572 pKa = 6.27 PMDD575 pKa = 3.93 NAEE578 pKa = 4.83 CGKK581 pKa = 10.05 IDD583 pKa = 5.15 DD584 pKa = 4.9 KK585 pKa = 11.5 DD586 pKa = 3.69 AGMFSGHH593 pKa = 5.75 SVGLPTPHH601 pKa = 6.9 TSTSFFYY608 pKa = 10.74 DD609 pKa = 3.36 RR610 pKa = 11.84 YY611 pKa = 9.76 RR612 pKa = 11.84 FVGIVKK618 pKa = 10.1 SVVNNTPKK626 pKa = 10.36 PVNIYY631 pKa = 11.04 DD632 pKa = 3.67 DD633 pKa = 3.59 TGKK636 pKa = 10.62 VKK638 pKa = 10.49 NLQQVFPTSDD648 pKa = 2.89 TLLPHH653 pKa = 6.77 SLMSLSPCASVCGQPISSFLFAQRR677 pKa = 11.84 ANPKK681 pKa = 8.58 KK682 pKa = 8.68 TLKK685 pKa = 10.38 LRR687 pKa = 11.84 SGDD690 pKa = 3.4 EE691 pKa = 3.79 FLYY694 pKa = 10.28 RR695 pKa = 11.84 CCPFSYY701 pKa = 10.0 IKK703 pKa = 10.45 CDD705 pKa = 3.46 LEE707 pKa = 4.38 FTVVPPANSTRR718 pKa = 11.84 DD719 pKa = 4.15 YY720 pKa = 10.49 IVHH723 pKa = 7.25 WYY725 pKa = 9.12 PPGATLDD732 pKa = 3.7 AGEE735 pKa = 4.29 VAVGNTSGSNGFDD748 pKa = 3.7 DD749 pKa = 4.75 NGMNAGSSLFSYY761 pKa = 10.83 NPTFHH766 pKa = 6.96 ARR768 pKa = 11.84 APSKK772 pKa = 10.63 VSAVIPFCLPVSLLPLYY789 pKa = 10.78 FDD791 pKa = 4.83 GFPDD795 pKa = 3.66 YY796 pKa = 10.84 STTKK800 pKa = 10.49 GMYY803 pKa = 8.88 GCSPSFSFGTIYY815 pKa = 10.27 IEE817 pKa = 4.68 SGLQEE822 pKa = 4.33 TYY824 pKa = 10.68 SVYY827 pKa = 10.31 IRR829 pKa = 11.84 YY830 pKa = 9.56 KK831 pKa = 10.37 DD832 pKa = 3.69 FKK834 pKa = 10.79 GYY836 pKa = 10.87 APRR839 pKa = 11.84 PLIRR843 pKa = 11.84 TPHH846 pKa = 5.5 IRR848 pKa = 11.84 LSEE851 pKa = 3.9 RR852 pKa = 11.84 ARR854 pKa = 11.84 YY855 pKa = 9.48 IMADD859 pKa = 3.2 SVLPRR864 pKa = 11.84 PLTRR868 pKa = 11.84 AEE870 pKa = 3.73 RR871 pKa = 11.84 DD872 pKa = 3.47 VARR875 pKa = 11.84 DD876 pKa = 3.43 LLLIAGDD883 pKa = 3.76 IEE885 pKa = 5.27 SNPGPAFNPEE895 pKa = 4.01 YY896 pKa = 9.13 TAHH899 pKa = 6.57 GPVTEE904 pKa = 5.45 LIQLARR910 pKa = 11.84 KK911 pKa = 9.31 PEE913 pKa = 4.09 TVDD916 pKa = 3.04 NVNRR920 pKa = 11.84 LLTTLNTLMAKK931 pKa = 9.25 WNNLKK936 pKa = 9.28 DD937 pKa = 3.81 TVTDD941 pKa = 3.8 AVFLRR946 pKa = 11.84 DD947 pKa = 4.01 MVCLLVKK954 pKa = 9.69 LTSLMYY960 pKa = 10.23 LVHH963 pKa = 6.11 GQGPGAYY970 pKa = 8.82 FAAASILLADD980 pKa = 5.8 GITFFDD986 pKa = 3.76 WYY988 pKa = 10.08 EE989 pKa = 3.97 KK990 pKa = 10.59 IKK992 pKa = 10.86 IFMARR997 pKa = 11.84 KK998 pKa = 9.43 LRR1000 pKa = 11.84 VSPPFFPAAQGPDD1013 pKa = 3.01 LRR1015 pKa = 11.84 DD1016 pKa = 3.1 FVTFFNAARR1025 pKa = 11.84 GAQWMIDD1032 pKa = 3.6 SLKK1035 pKa = 11.18 SLITCIKK1042 pKa = 9.21 QWLEE1046 pKa = 3.8 LEE1048 pKa = 4.4 EE1049 pKa = 4.2 EE1050 pKa = 4.33 NEE1052 pKa = 4.05 AVQLEE1057 pKa = 4.41 KK1058 pKa = 10.56 MLIDD1062 pKa = 4.15 SPRR1065 pKa = 11.84 HH1066 pKa = 5.01 CKK1068 pKa = 10.32 AINDD1072 pKa = 3.9 YY1073 pKa = 11.24 NRR1075 pKa = 11.84 GDD1077 pKa = 3.56 SFQRR1081 pKa = 11.84 PTNSFEE1087 pKa = 4.03 FMDD1090 pKa = 4.42 RR1091 pKa = 11.84 LVEE1094 pKa = 4.14 CATKK1098 pKa = 10.46 LGKK1101 pKa = 9.82 VQIATYY1107 pKa = 9.9 FRR1109 pKa = 11.84 NFTTADD1115 pKa = 3.42 SDD1117 pKa = 3.76 TSRR1120 pKa = 11.84 PEE1122 pKa = 3.56 PVVVVLRR1129 pKa = 11.84 GKK1131 pKa = 9.88 PGVGKK1136 pKa = 10.22 SAAATVMAAAVSKK1149 pKa = 10.64 LLVGSQSVYY1158 pKa = 10.15 TLSPDD1163 pKa = 3.6 TEE1165 pKa = 4.51 HH1166 pKa = 6.78 MDD1168 pKa = 3.77 GYY1170 pKa = 10.59 HH1171 pKa = 5.7 GQFVTLMDD1179 pKa = 5.37 DD1180 pKa = 4.56 LGQNPDD1186 pKa = 3.91 GEE1188 pKa = 4.55 DD1189 pKa = 3.66 FRR1191 pKa = 11.84 CFCQMVSCAQYY1202 pKa = 10.05 RR1203 pKa = 11.84 PAMADD1208 pKa = 3.39 LKK1210 pKa = 11.27 DD1211 pKa = 3.54 KK1212 pKa = 11.42 GILFTSRR1219 pKa = 11.84 LLIATTNLPDD1229 pKa = 4.43 FNPVTISDD1237 pKa = 3.62 PRR1239 pKa = 11.84 ALDD1242 pKa = 3.27 RR1243 pKa = 11.84 RR1244 pKa = 11.84 ITFDD1248 pKa = 3.01 ILVTPGSAATKK1259 pKa = 10.28 NGKK1262 pKa = 9.44 LDD1264 pKa = 3.43 LAAALKK1270 pKa = 10.15 PDD1272 pKa = 3.77 GPGEE1276 pKa = 4.0 HH1277 pKa = 7.72 PYY1279 pKa = 10.74 TSDD1282 pKa = 4.19 CPILHH1287 pKa = 5.53 TTGLLLKK1294 pKa = 10.27 NLRR1297 pKa = 11.84 NNQTMNLKK1305 pKa = 10.45 DD1306 pKa = 4.09 LVDD1309 pKa = 4.5 MIVKK1313 pKa = 10.32 RR1314 pKa = 11.84 IKK1316 pKa = 10.17 HH1317 pKa = 5.4 KK1318 pKa = 11.1 KK1319 pKa = 8.58 EE1320 pKa = 3.62 VGNMLDD1326 pKa = 3.38 SLVAQGPTMIVGYY1339 pKa = 8.73 TKK1341 pKa = 10.88 DD1342 pKa = 3.7 DD1343 pKa = 3.99 DD1344 pKa = 5.14 GIAIVDD1350 pKa = 5.04 CLEE1353 pKa = 3.81 EE1354 pKa = 4.0 WNKK1357 pKa = 10.37 IKK1359 pKa = 10.58 DD1360 pKa = 3.68 KK1361 pKa = 10.93 KK1362 pKa = 10.54 KK1363 pKa = 10.12 KK1364 pKa = 9.72 QLALEE1369 pKa = 4.3 MVAQEE1374 pKa = 4.87 LKK1376 pKa = 10.73 DD1377 pKa = 3.14 KK1378 pKa = 11.17 HH1379 pKa = 6.42 EE1380 pKa = 4.12 EE1381 pKa = 3.92 HH1382 pKa = 6.98 KK1383 pKa = 10.77 GTIKK1387 pKa = 10.3 LLKK1390 pKa = 9.96 MFVTGLGVVAAVAGAYY1406 pKa = 8.54 ATMKK1410 pKa = 10.53 YY1411 pKa = 7.49 FTKK1414 pKa = 10.49 DD1415 pKa = 2.9 KK1416 pKa = 10.42 PKK1418 pKa = 10.67 EE1419 pKa = 4.01 EE1420 pKa = 4.18 EE1421 pKa = 4.11 EE1422 pKa = 4.06 EE1423 pKa = 4.27 PEE1425 pKa = 3.95 EE1426 pKa = 4.18 KK1427 pKa = 10.23 KK1428 pKa = 10.62 EE1429 pKa = 4.15 KK1430 pKa = 9.4 KK1431 pKa = 9.08 TEE1433 pKa = 3.98 EE1434 pKa = 4.23 SKK1436 pKa = 10.93 EE1437 pKa = 3.66 AAGPYY1442 pKa = 9.7 NGPTKK1447 pKa = 10.73 KK1448 pKa = 10.18 EE1449 pKa = 3.75 IKK1451 pKa = 8.92 TLKK1454 pKa = 10.52 LKK1456 pKa = 10.54 AQSPLMDD1463 pKa = 3.75 MEE1465 pKa = 5.07 KK1466 pKa = 10.51 KK1467 pKa = 9.74 IAQNVMPFQIFYY1479 pKa = 10.15 NGKK1482 pKa = 10.16 RR1483 pKa = 11.84 YY1484 pKa = 7.02 TQSCLAIGKK1493 pKa = 9.4 RR1494 pKa = 11.84 VILVNKK1500 pKa = 9.56 HH1501 pKa = 5.21 AFEE1504 pKa = 4.28 SVEE1507 pKa = 4.01 HH1508 pKa = 6.54 KK1509 pKa = 10.72 FVVDD1513 pKa = 3.56 QKK1515 pKa = 11.3 EE1516 pKa = 4.1 YY1517 pKa = 10.4 TLDD1520 pKa = 3.27 QVTAISLDD1528 pKa = 3.8 CGSGVTDD1535 pKa = 3.4 VCAVCLPPGPDD1546 pKa = 3.05 FKK1548 pKa = 11.19 SIKK1551 pKa = 10.01 KK1552 pKa = 9.85 HH1553 pKa = 5.15 FLPFNTTMFPGTRR1566 pKa = 11.84 LTILSNDD1573 pKa = 3.97 HH1574 pKa = 6.4 YY1575 pKa = 10.84 PMSRR1579 pKa = 11.84 EE1580 pKa = 3.68 GSFLRR1585 pKa = 11.84 FEE1587 pKa = 5.19 DD1588 pKa = 4.27 EE1589 pKa = 4.2 VPTNVGNMPFVMLYY1603 pKa = 10.63 KK1604 pKa = 8.86 STSYY1608 pKa = 10.94 FGMCGSVVCSRR1619 pKa = 11.84 FVDD1622 pKa = 3.63 GGGIIGMHH1630 pKa = 6.46 CAGGGGVSVGTRR1642 pKa = 11.84 LTARR1646 pKa = 11.84 MIEE1649 pKa = 4.23 SVFDD1653 pKa = 3.36 YY1654 pKa = 9.25 FYY1656 pKa = 10.86 PPVAQGIIEE1665 pKa = 4.1 NTEE1668 pKa = 3.62 TGPRR1672 pKa = 11.84 VHH1674 pKa = 6.75 VPRR1677 pKa = 11.84 TSKK1680 pKa = 10.76 LKK1682 pKa = 9.56 RR1683 pKa = 11.84 TNATYY1688 pKa = 8.91 PATEE1692 pKa = 4.33 KK1693 pKa = 10.78 YY1694 pKa = 10.62 GPAALSRR1701 pKa = 11.84 YY1702 pKa = 9.53 DD1703 pKa = 3.27 PRR1705 pKa = 11.84 LNEE1708 pKa = 3.99 GVNLDD1713 pKa = 3.47 EE1714 pKa = 5.67 VIFSKK1719 pKa = 8.98 HH1720 pKa = 4.04 TQNTLVEE1727 pKa = 4.39 KK1728 pKa = 10.9 GSTFRR1733 pKa = 11.84 SALDD1737 pKa = 3.46 MAAEE1741 pKa = 4.29 IYY1743 pKa = 10.66 GEE1745 pKa = 4.14 KK1746 pKa = 10.52 FRR1748 pKa = 11.84 GNDD1751 pKa = 3.59 FSPLSVEE1758 pKa = 4.35 DD1759 pKa = 5.12 AILGIPGLDD1768 pKa = 2.74 RR1769 pKa = 11.84 LDD1771 pKa = 4.44 PNTASGLPYY1780 pKa = 10.17 TKK1782 pKa = 9.69 TRR1784 pKa = 11.84 RR1785 pKa = 11.84 QMIDD1789 pKa = 3.19 FNTGQILDD1797 pKa = 4.04 DD1798 pKa = 3.77 TLKK1801 pKa = 10.81 CRR1803 pKa = 11.84 LGQWLAGRR1811 pKa = 11.84 PPQEE1815 pKa = 3.37 VHH1817 pKa = 5.59 YY1818 pKa = 9.24 QTFLKK1823 pKa = 10.93 DD1824 pKa = 3.64 EE1825 pKa = 4.03 IRR1827 pKa = 11.84 PIEE1830 pKa = 4.16 KK1831 pKa = 10.27 VKK1833 pKa = 10.79 AGKK1836 pKa = 7.9 TRR1838 pKa = 11.84 IIDD1841 pKa = 3.9 VPPLDD1846 pKa = 3.69 HH1847 pKa = 7.18 VIAFRR1852 pKa = 11.84 MLFGRR1857 pKa = 11.84 FIAHH1861 pKa = 5.05 YY1862 pKa = 9.5 HH1863 pKa = 5.98 LNFGFKK1869 pKa = 9.07 TGSAIGCDD1877 pKa = 3.86 PDD1879 pKa = 3.85 VAWASFGFEE1888 pKa = 3.95 LSGFPYY1894 pKa = 10.39 LYY1896 pKa = 11.01 DD1897 pKa = 3.56 FDD1899 pKa = 4.97 YY1900 pKa = 11.67 SNFDD1904 pKa = 3.55 ASHH1907 pKa = 5.52 STSIFEE1913 pKa = 4.13 ILEE1916 pKa = 3.91 QKK1918 pKa = 10.39 FFSPEE1923 pKa = 3.61 LGFDD1927 pKa = 3.78 PRR1929 pKa = 11.84 CSLLLKK1935 pKa = 10.33 SLAVSTHH1942 pKa = 5.95 CYY1944 pKa = 8.41 EE1945 pKa = 4.26 NKK1947 pKa = 9.91 RR1948 pKa = 11.84 LQIAGGLPSGTAGTSVLNTVINNIIFHH1975 pKa = 6.35 GALYY1979 pKa = 8.48 HH1980 pKa = 6.61 TYY1982 pKa = 10.79 TNFEE1986 pKa = 4.04 RR1987 pKa = 11.84 DD1988 pKa = 4.55 DD1989 pKa = 4.13 ISMLAYY1995 pKa = 10.42 GDD1997 pKa = 4.92 DD1998 pKa = 3.15 IVVASKK2004 pKa = 11.22 FEE2006 pKa = 4.49 LDD2008 pKa = 3.44 LVMVKK2013 pKa = 10.9 AFMNRR2018 pKa = 11.84 IGYY2021 pKa = 9.68 KK2022 pKa = 8.63 ITPADD2027 pKa = 3.51 KK2028 pKa = 10.75 SDD2030 pKa = 3.46 EE2031 pKa = 4.03 FRR2033 pKa = 11.84 PKK2035 pKa = 10.81 CMDD2038 pKa = 5.12 DD2039 pKa = 2.85 ICFLKK2044 pKa = 10.63 RR2045 pKa = 11.84 RR2046 pKa = 11.84 FVKK2049 pKa = 10.62 VAGVWAPVMEE2059 pKa = 4.42 TEE2061 pKa = 4.36 NLEE2064 pKa = 5.53 AMLSWYY2070 pKa = 10.36 KK2071 pKa = 10.66 PGTLNEE2077 pKa = 4.2 KK2078 pKa = 9.44 LQSVSRR2084 pKa = 11.84 LAHH2087 pKa = 5.46 FSGRR2091 pKa = 11.84 DD2092 pKa = 3.57 VYY2094 pKa = 11.78 DD2095 pKa = 3.5 HH2096 pKa = 6.89 LFKK2099 pKa = 10.98 PFIRR2103 pKa = 11.84 DD2104 pKa = 3.53 GFDD2107 pKa = 3.03 VTPWKK2112 pKa = 10.04 QLHH2115 pKa = 6.23 LEE2117 pKa = 4.12 WLNKK2121 pKa = 10.04 LSAA2124 pKa = 4.12
Molecular weight: 235.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.953
IPC2_protein 5.944
IPC_protein 6.02
Toseland 6.351
ProMoST 6.415
Dawson 6.326
Bjellqvist 6.338
Wikipedia 6.351
Rodwell 6.313
Grimsley 6.491
Solomon 6.326
Lehninger 6.326
Nozaki 6.62
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.707
Patrickios 4.113
IPC_peptide 6.351
IPC2_peptide 6.62
IPC2.peptide.svr19 6.645
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
2124
2124
2124
2124.0
235.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.874 ± 0.0
1.554 ± 0.0
5.932 ± 0.0
4.614 ± 0.0
5.791 ± 0.0
6.685 ± 0.0
2.213 ± 0.0
4.755 ± 0.0
5.603 ± 0.0
8.757 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.872 ± 0.0
4.849 ± 0.0
6.356 ± 0.0
2.919 ± 0.0
4.19 ± 0.0
7.392 ± 0.0
7.486 ± 0.0
6.733 ± 0.0
0.895 ± 0.0
3.531 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here