Anoxybacillus sp. B7M1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus; unclassified Anoxybacillus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160FDR0|A0A160FDR0_9BACI Putative siderophore transport system ATP-binding protein YusV OS=Anoxybacillus sp. B7M1 OX=1490057 GN=GFC29_1176 PE=4 SV=1
MM1 pKa = 7.59SLPNIPNITPIISLEE16 pKa = 3.92RR17 pKa = 11.84CEE19 pKa = 4.92TIDD22 pKa = 5.24LLLSSIALEE31 pKa = 4.48EE32 pKa = 4.32IGLSNILNAEE42 pKa = 4.01AEE44 pKa = 4.19KK45 pKa = 10.91LQAFLNTDD53 pKa = 3.67PNNLNDD59 pKa = 3.61YY60 pKa = 10.91LKK62 pKa = 10.6INEE65 pKa = 4.64SINRR69 pKa = 11.84TLRR72 pKa = 11.84AVVKK76 pKa = 10.32SQILLQFKK84 pKa = 10.96LEE86 pKa = 4.85DD87 pKa = 4.46IILLDD92 pKa = 5.35RR93 pKa = 11.84DD94 pKa = 4.32TCCEE98 pKa = 3.98KK99 pKa = 10.95CPSQDD104 pKa = 3.18PDD106 pKa = 3.53EE107 pKa = 5.79CDD109 pKa = 3.82EE110 pKa = 4.82EE111 pKa = 4.98PPCPDD116 pKa = 3.79CLDD119 pKa = 3.5RR120 pKa = 11.84YY121 pKa = 9.39YY122 pKa = 10.42PCGCDD127 pKa = 3.24EE128 pKa = 4.2EE129 pKa = 4.83

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167UEJ5|A0A167UEJ5_9BACI Staygreen family protein OS=Anoxybacillus sp. B7M1 OX=1490057 GN=GFC29_194 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3736

0

3736

1044184

29

2387

279.5

31.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.763 ± 0.044

0.814 ± 0.013

4.63 ± 0.032

7.411 ± 0.052

4.414 ± 0.034

6.742 ± 0.043

2.421 ± 0.022

7.467 ± 0.042

6.558 ± 0.044

9.887 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.022

3.707 ± 0.037

3.806 ± 0.024

4.243 ± 0.036

4.712 ± 0.029

5.498 ± 0.032

5.183 ± 0.035

7.077 ± 0.033

1.157 ± 0.017

3.622 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski