Amino acid dipepetide frequency for Anoxybacillus sp. B7M1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.226AlaAla: 7.226 ± 0.104
0.732AlaCys: 0.732 ± 0.029
3.526AlaAsp: 3.526 ± 0.052
5.225AlaGlu: 5.225 ± 0.083
3.529AlaPhe: 3.529 ± 0.064
5.329AlaGly: 5.329 ± 0.075
1.666AlaHis: 1.666 ± 0.041
6.139AlaIle: 6.139 ± 0.084
5.456AlaLys: 5.456 ± 0.081
8.143AlaLeu: 8.143 ± 0.084
2.207AlaMet: 2.207 ± 0.048
2.887AlaAsn: 2.887 ± 0.058
2.341AlaPro: 2.341 ± 0.057
2.78AlaGln: 2.78 ± 0.054
3.227AlaArg: 3.227 ± 0.06
4.152AlaSer: 4.152 ± 0.066
3.685AlaThr: 3.685 ± 0.068
6.045AlaVal: 6.045 ± 0.099
0.715AlaTrp: 0.715 ± 0.027
2.625AlaTyr: 2.625 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.523CysAla: 0.523 ± 0.023
0.132CysCys: 0.132 ± 0.013
0.409CysAsp: 0.409 ± 0.02
0.604CysGlu: 0.604 ± 0.026
0.401CysPhe: 0.401 ± 0.021
0.728CysGly: 0.728 ± 0.031
0.233CysHis: 0.233 ± 0.017
0.544CysIle: 0.544 ± 0.021
0.333CysLys: 0.333 ± 0.019
0.785CysLeu: 0.785 ± 0.03
0.2CysMet: 0.2 ± 0.014
0.253CysAsn: 0.253 ± 0.019
0.419CysPro: 0.419 ± 0.02
0.293CysGln: 0.293 ± 0.017
0.397CysArg: 0.397 ± 0.02
0.539CysSer: 0.539 ± 0.02
0.426CysThr: 0.426 ± 0.02
0.501CysVal: 0.501 ± 0.022
0.087CysTrp: 0.087 ± 0.011
0.328CysTyr: 0.328 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.137AspAla: 3.137 ± 0.059
0.386AspCys: 0.386 ± 0.02
2.24AspAsp: 2.24 ± 0.047
4.191AspGlu: 4.191 ± 0.066
2.106AspPhe: 2.106 ± 0.042
3.249AspGly: 3.249 ± 0.068
1.271AspHis: 1.271 ± 0.039
3.694AspIle: 3.694 ± 0.06
2.227AspLys: 2.227 ± 0.049
4.482AspLeu: 4.482 ± 0.068
1.235AspMet: 1.235 ± 0.033
1.264AspAsn: 1.264 ± 0.044
2.14AspPro: 2.14 ± 0.048
1.824AspGln: 1.824 ± 0.043
2.444AspArg: 2.444 ± 0.053
2.035AspSer: 2.035 ± 0.048
2.035AspThr: 2.035 ± 0.045
3.732AspVal: 3.732 ± 0.069
0.634AspTrp: 0.634 ± 0.025
1.972AspTyr: 1.972 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.279GluAla: 6.279 ± 0.092
0.441GluCys: 0.441 ± 0.026
2.934GluAsp: 2.934 ± 0.06
6.693GluGlu: 6.693 ± 0.091
2.379GluPhe: 2.379 ± 0.051
4.027GluGly: 4.027 ± 0.071
1.727GluHis: 1.727 ± 0.048
5.323GluIle: 5.323 ± 0.078
6.566GluLys: 6.566 ± 0.088
6.947GluLeu: 6.947 ± 0.087
2.457GluMet: 2.457 ± 0.049
2.983GluAsn: 2.983 ± 0.059
2.082GluPro: 2.082 ± 0.039
4.363GluGln: 4.363 ± 0.077
4.332GluArg: 4.332 ± 0.075
2.829GluSer: 2.829 ± 0.048
3.807GluThr: 3.807 ± 0.057
4.762GluVal: 4.762 ± 0.07
0.983GluTrp: 0.983 ± 0.032
2.201GluTyr: 2.201 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
3.452PheAla: 3.452 ± 0.065
0.398PheCys: 0.398 ± 0.021
2.156PheAsp: 2.156 ± 0.052
2.422PheGlu: 2.422 ± 0.055
2.402PhePhe: 2.402 ± 0.072
3.157PheGly: 3.157 ± 0.06
1.217PheHis: 1.217 ± 0.037
3.442PheIle: 3.442 ± 0.071
1.846PheLys: 1.846 ± 0.048
4.66PheLeu: 4.66 ± 0.088
1.223PheMet: 1.223 ± 0.036
1.365PheAsn: 1.365 ± 0.043
1.743PhePro: 1.743 ± 0.042
1.815PheGln: 1.815 ± 0.044
1.842PheArg: 1.842 ± 0.048
3.142PheSer: 3.142 ± 0.057
2.365PheThr: 2.365 ± 0.044
3.27PheVal: 3.27 ± 0.063
0.528PheTrp: 0.528 ± 0.026
1.692PheTyr: 1.692 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.182GlyAla: 5.182 ± 0.089
0.687GlyCys: 0.687 ± 0.024
2.972GlyAsp: 2.972 ± 0.052
4.464GlyGlu: 4.464 ± 0.07
3.145GlyPhe: 3.145 ± 0.052
4.626GlyGly: 4.626 ± 0.074
1.441GlyHis: 1.441 ± 0.038
5.625GlyIle: 5.625 ± 0.083
4.91GlyLys: 4.91 ± 0.068
6.281GlyLeu: 6.281 ± 0.086
2.265GlyMet: 2.265 ± 0.05
2.496GlyAsn: 2.496 ± 0.057
1.754GlyPro: 1.754 ± 0.056
2.352GlyGln: 2.352 ± 0.046
2.981GlyArg: 2.981 ± 0.051
3.42GlySer: 3.42 ± 0.055
3.995GlyThr: 3.995 ± 0.066
5.0GlyVal: 5.0 ± 0.076
0.917GlyTrp: 0.917 ± 0.033
2.908GlyTyr: 2.908 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.593HisAla: 1.593 ± 0.044
0.244HisCys: 0.244 ± 0.014
1.147HisAsp: 1.147 ± 0.032
1.668HisGlu: 1.668 ± 0.042
1.148HisPhe: 1.148 ± 0.037
1.567HisGly: 1.567 ± 0.041
0.971HisHis: 0.971 ± 0.041
1.791HisIle: 1.791 ± 0.042
1.023HisLys: 1.023 ± 0.031
2.567HisLeu: 2.567 ± 0.05
0.669HisMet: 0.669 ± 0.024
0.668HisAsn: 0.668 ± 0.026
1.416HisPro: 1.416 ± 0.037
1.052HisGln: 1.052 ± 0.029
1.148HisArg: 1.148 ± 0.034
1.363HisSer: 1.363 ± 0.038
1.06HisThr: 1.06 ± 0.031
1.801HisVal: 1.801 ± 0.042
0.29HisTrp: 0.29 ± 0.017
1.027HisTyr: 1.027 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.529IleAla: 6.529 ± 0.082
0.713IleCys: 0.713 ± 0.024
4.067IleAsp: 4.067 ± 0.06
5.717IleGlu: 5.717 ± 0.083
2.988IlePhe: 2.988 ± 0.07
5.952IleGly: 5.952 ± 0.088
1.961IleHis: 1.961 ± 0.045
5.079IleIle: 5.079 ± 0.085
3.814IleLys: 3.814 ± 0.071
6.325IleLeu: 6.325 ± 0.099
1.696IleMet: 1.696 ± 0.038
2.421IleAsn: 2.421 ± 0.053
3.339IlePro: 3.339 ± 0.055
3.216IleGln: 3.216 ± 0.065
3.659IleArg: 3.659 ± 0.065
4.495IleSer: 4.495 ± 0.065
3.761IleThr: 3.761 ± 0.064
5.72IleVal: 5.72 ± 0.073
0.732IleTrp: 0.732 ± 0.027
2.487IleTyr: 2.487 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.9LysAla: 4.9 ± 0.077
0.327LysCys: 0.327 ± 0.019
2.904LysAsp: 2.904 ± 0.062
6.522LysGlu: 6.522 ± 0.083
1.626LysPhe: 1.626 ± 0.039
4.453LysGly: 4.453 ± 0.072
1.38LysHis: 1.38 ± 0.039
4.269LysIle: 4.269 ± 0.068
5.629LysLys: 5.629 ± 0.093
5.402LysLeu: 5.402 ± 0.081
2.18LysMet: 2.18 ± 0.044
2.787LysAsn: 2.787 ± 0.061
2.162LysPro: 2.162 ± 0.052
3.764LysGln: 3.764 ± 0.07
3.887LysArg: 3.887 ± 0.054
2.754LysSer: 2.754 ± 0.057
3.515LysThr: 3.515 ± 0.064
4.154LysVal: 4.154 ± 0.065
0.983LysTrp: 0.983 ± 0.031
1.981LysTyr: 1.981 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
8.252LeuAla: 8.252 ± 0.089
0.807LeuCys: 0.807 ± 0.03
4.416LeuAsp: 4.416 ± 0.066
6.561LeuGlu: 6.561 ± 0.085
4.858LeuPhe: 4.858 ± 0.089
6.254LeuGly: 6.254 ± 0.095
2.406LeuHis: 2.406 ± 0.052
6.515LeuIle: 6.515 ± 0.107
6.257LeuLys: 6.257 ± 0.088
11.001LeuLeu: 11.001 ± 0.168
2.497LeuMet: 2.497 ± 0.052
3.567LeuAsn: 3.567 ± 0.064
4.247LeuPro: 4.247 ± 0.066
4.284LeuGln: 4.284 ± 0.079
4.358LeuArg: 4.358 ± 0.076
6.636LeuSer: 6.636 ± 0.083
5.27LeuThr: 5.27 ± 0.078
6.395LeuVal: 6.395 ± 0.09
0.912LeuTrp: 0.912 ± 0.033
3.374LeuTyr: 3.374 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.308MetAla: 2.308 ± 0.052
0.176MetCys: 0.176 ± 0.013
1.371MetAsp: 1.371 ± 0.034
2.159MetGlu: 2.159 ± 0.043
1.061MetPhe: 1.061 ± 0.034
1.696MetGly: 1.696 ± 0.04
0.467MetHis: 0.467 ± 0.021
2.41MetIle: 2.41 ± 0.048
2.656MetLys: 2.656 ± 0.05
2.705MetLeu: 2.705 ± 0.054
1.075MetMet: 1.075 ± 0.035
1.534MetAsn: 1.534 ± 0.037
0.963MetPro: 0.963 ± 0.034
0.967MetGln: 0.967 ± 0.031
1.297MetArg: 1.297 ± 0.035
1.565MetSer: 1.565 ± 0.036
1.645MetThr: 1.645 ± 0.041
1.847MetVal: 1.847 ± 0.04
0.209MetTrp: 0.209 ± 0.014
0.755MetTyr: 0.755 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.565AsnAla: 2.565 ± 0.053
0.255AsnCys: 0.255 ± 0.015
1.854AsnAsp: 1.854 ± 0.042
3.259AsnGlu: 3.259 ± 0.057
1.256AsnPhe: 1.256 ± 0.04
3.335AsnGly: 3.335 ± 0.066
0.958AsnHis: 0.958 ± 0.033
2.784AsnIle: 2.784 ± 0.063
2.197AsnLys: 2.197 ± 0.049
2.897AsnLeu: 2.897 ± 0.056
1.033AsnMet: 1.033 ± 0.033
1.414AsnAsn: 1.414 ± 0.054
1.903AsnPro: 1.903 ± 0.043
1.654AsnGln: 1.654 ± 0.049
1.974AsnArg: 1.974 ± 0.048
1.686AsnSer: 1.686 ± 0.049
1.677AsnThr: 1.677 ± 0.047
2.714AsnVal: 2.714 ± 0.057
0.453AsnTrp: 0.453 ± 0.022
1.205AsnTyr: 1.205 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.518ProAla: 2.518 ± 0.054
0.25ProCys: 0.25 ± 0.015
1.967ProAsp: 1.967 ± 0.049
2.811ProGlu: 2.811 ± 0.058
2.212ProPhe: 2.212 ± 0.048
2.208ProGly: 2.208 ± 0.044
1.033ProHis: 1.033 ± 0.035
2.939ProIle: 2.939 ± 0.056
2.361ProLys: 2.361 ± 0.051
4.037ProLeu: 4.037 ± 0.064
0.874ProMet: 0.874 ± 0.03
1.717ProAsn: 1.717 ± 0.044
1.223ProPro: 1.223 ± 0.036
1.215ProGln: 1.215 ± 0.035
1.334ProArg: 1.334 ± 0.039
2.539ProSer: 2.539 ± 0.056
2.048ProThr: 2.048 ± 0.049
2.827ProVal: 2.827 ± 0.048
0.397ProTrp: 0.397 ± 0.019
1.551ProTyr: 1.551 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.457GlnAla: 3.457 ± 0.061
0.25GlnCys: 0.25 ± 0.016
1.504GlnAsp: 1.504 ± 0.034
3.217GlnGlu: 3.217 ± 0.06
1.705GlnPhe: 1.705 ± 0.037
2.266GlnGly: 2.266 ± 0.04
1.04GlnHis: 1.04 ± 0.033
2.784GlnIle: 2.784 ± 0.051
3.153GlnLys: 3.153 ± 0.053
4.826GlnLeu: 4.826 ± 0.078
1.416GlnMet: 1.416 ± 0.045
1.477GlnAsn: 1.477 ± 0.039
1.599GlnPro: 1.599 ± 0.045
2.479GlnGln: 2.479 ± 0.066
2.061GlnArg: 2.061 ± 0.044
2.151GlnSer: 2.151 ± 0.045
2.47GlnThr: 2.47 ± 0.052
2.433GlnVal: 2.433 ± 0.053
0.587GlnTrp: 0.587 ± 0.026
1.556GlnTyr: 1.556 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
3.012ArgAla: 3.012 ± 0.056
0.4ArgCys: 0.4 ± 0.023
2.125ArgAsp: 2.125 ± 0.045
3.608ArgGlu: 3.608 ± 0.061
2.297ArgPhe: 2.297 ± 0.045
2.593ArgGly: 2.593 ± 0.051
1.111ArgHis: 1.111 ± 0.031
3.476ArgIle: 3.476 ± 0.056
3.442ArgLys: 3.442 ± 0.059
5.064ArgLeu: 5.064 ± 0.078
1.604ArgMet: 1.604 ± 0.04
1.781ArgAsn: 1.781 ± 0.045
1.652ArgPro: 1.652 ± 0.043
2.188ArgGln: 2.188 ± 0.05
2.362ArgArg: 2.362 ± 0.054
2.478ArgSer: 2.478 ± 0.055
2.428ArgThr: 2.428 ± 0.045
2.949ArgVal: 2.949 ± 0.046
0.57ArgTrp: 0.57 ± 0.024
1.977ArgTyr: 1.977 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
3.834SerAla: 3.834 ± 0.072
0.458SerCys: 0.458 ± 0.023
2.456SerAsp: 2.456 ± 0.056
3.441SerGlu: 3.441 ± 0.059
3.209SerPhe: 3.209 ± 0.061
4.135SerGly: 4.135 ± 0.059
1.334SerHis: 1.334 ± 0.036
4.387SerIle: 4.387 ± 0.065
2.972SerLys: 2.972 ± 0.058
6.11SerLeu: 6.11 ± 0.084
1.573SerMet: 1.573 ± 0.042
1.783SerAsn: 1.783 ± 0.046
2.179SerPro: 2.179 ± 0.046
1.947SerGln: 1.947 ± 0.043
2.323SerArg: 2.323 ± 0.052
3.554SerSer: 3.554 ± 0.06
2.514SerThr: 2.514 ± 0.053
3.926SerVal: 3.926 ± 0.06
0.668SerTrp: 0.668 ± 0.029
2.181SerTyr: 2.181 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
4.132ThrAla: 4.132 ± 0.063
0.359ThrCys: 0.359 ± 0.021
2.383ThrAsp: 2.383 ± 0.052
3.261ThrGlu: 3.261 ± 0.054
2.478ThrPhe: 2.478 ± 0.053
3.995ThrGly: 3.995 ± 0.095
1.107ThrHis: 1.107 ± 0.034
4.407ThrIle: 4.407 ± 0.064
3.438ThrLys: 3.438 ± 0.067
4.961ThrLeu: 4.961 ± 0.068
1.366ThrMet: 1.366 ± 0.033
2.213ThrAsn: 2.213 ± 0.05
2.175ThrPro: 2.175 ± 0.045
1.389ThrGln: 1.389 ± 0.037
1.85ThrArg: 1.85 ± 0.046
2.844ThrSer: 2.844 ± 0.056
2.708ThrThr: 2.708 ± 0.064
4.219ThrVal: 4.219 ± 0.071
0.557ThrTrp: 0.557 ± 0.023
1.984ThrTyr: 1.984 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
5.666ValAla: 5.666 ± 0.089
0.671ValCys: 0.671 ± 0.023
3.343ValAsp: 3.343 ± 0.055
4.627ValGlu: 4.627 ± 0.074
3.037ValPhe: 3.037 ± 0.055
4.542ValGly: 4.542 ± 0.066
1.66ValHis: 1.66 ± 0.042
5.575ValIle: 5.575 ± 0.069
4.554ValLys: 4.554 ± 0.08
6.91ValLeu: 6.91 ± 0.097
1.895ValMet: 1.895 ± 0.047
2.668ValAsn: 2.668 ± 0.057
2.917ValPro: 2.917 ± 0.056
2.783ValGln: 2.783 ± 0.055
3.081ValArg: 3.081 ± 0.053
4.281ValSer: 4.281 ± 0.072
3.979ValThr: 3.979 ± 0.072
5.336ValVal: 5.336 ± 0.082
0.728ValTrp: 0.728 ± 0.03
2.52ValTyr: 2.52 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.72TrpAla: 0.72 ± 0.03
0.092TrpCys: 0.092 ± 0.01
0.484TrpAsp: 0.484 ± 0.022
0.756TrpGlu: 0.756 ± 0.029
0.536TrpPhe: 0.536 ± 0.027
0.753TrpGly: 0.753 ± 0.028
0.266TrpHis: 0.266 ± 0.02
0.955TrpIle: 0.955 ± 0.035
0.859TrpLys: 0.859 ± 0.026
1.413TrpLeu: 1.413 ± 0.038
0.454TrpMet: 0.454 ± 0.02
0.574TrpAsn: 0.574 ± 0.023
0.332TrpPro: 0.332 ± 0.02
0.463TrpGln: 0.463 ± 0.021
0.548TrpArg: 0.548 ± 0.025
0.58TrpSer: 0.58 ± 0.028
0.607TrpThr: 0.607 ± 0.029
0.629TrpVal: 0.629 ± 0.028
0.18TrpTrp: 0.18 ± 0.015
0.37TrpTyr: 0.37 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.351TyrAla: 2.351 ± 0.048
0.358TyrCys: 0.358 ± 0.02
2.042TyrAsp: 2.042 ± 0.068
2.908TyrGlu: 2.908 ± 0.057
1.763TyrPhe: 1.763 ± 0.043
2.556TyrGly: 2.556 ± 0.049
0.964TyrHis: 0.964 ± 0.029
2.535TyrIle: 2.535 ± 0.053
1.889TyrLys: 1.889 ± 0.051
3.352TyrLeu: 3.352 ± 0.063
0.946TyrMet: 0.946 ± 0.032
1.218TyrAsn: 1.218 ± 0.041
1.475TyrPro: 1.475 ± 0.041
1.603TyrGln: 1.603 ± 0.04
2.011TyrArg: 2.011 ± 0.045
1.939TyrSer: 1.939 ± 0.051
1.831TyrThr: 1.831 ± 0.045
2.51TyrVal: 2.51 ± 0.047
0.441TyrTrp: 0.441 ± 0.022
1.528TyrTyr: 1.528 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3736 proteins (1044184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski