Hubei sobemo-like virus 26

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE52|A0A1L3KE52_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 26 OX=1923212 PE=4 SV=1
MM1 pKa = 7.27RR2 pKa = 11.84QAVLDD7 pKa = 4.47ASCQAYY13 pKa = 8.97EE14 pKa = 3.94AARR17 pKa = 11.84WRR19 pKa = 11.84IPSDD23 pKa = 3.44FLQRR27 pKa = 11.84SHH29 pKa = 7.52FDD31 pKa = 2.86RR32 pKa = 11.84VVQNLDD38 pKa = 3.03WTSSPGYY45 pKa = 9.33PFLLRR50 pKa = 11.84APNNKK55 pKa = 9.07VLFGTDD61 pKa = 3.05DD62 pKa = 3.4QGCVDD67 pKa = 4.01EE68 pKa = 5.6AKK70 pKa = 10.81ASIIWQIVQDD80 pKa = 4.4RR81 pKa = 11.84ISGKK85 pKa = 10.04EE86 pKa = 3.35KK87 pKa = 10.31AGYY90 pKa = 9.05IRR92 pKa = 11.84LFIKK96 pKa = 10.71PEE98 pKa = 3.27AHH100 pKa = 5.77TAKK103 pKa = 10.56KK104 pKa = 10.32LADD107 pKa = 3.31EE108 pKa = 4.97RR109 pKa = 11.84YY110 pKa = 9.91RR111 pKa = 11.84LISSVNVVDD120 pKa = 4.94QIVDD124 pKa = 3.21HH125 pKa = 6.27MLFGDD130 pKa = 3.79MNGTCIANWPFIPSKK145 pKa = 10.08PGWSIAKK152 pKa = 9.8GGWRR156 pKa = 11.84FMPSEE161 pKa = 3.71VWMATDD167 pKa = 4.0ASAWDD172 pKa = 3.38WTVRR176 pKa = 11.84PWLLEE181 pKa = 3.69MALEE185 pKa = 4.9LRR187 pKa = 11.84MLLCDD192 pKa = 3.86NLSEE196 pKa = 4.22QWSTLATRR204 pKa = 11.84RR205 pKa = 11.84YY206 pKa = 9.94RR207 pKa = 11.84EE208 pKa = 4.73LFTQPEE214 pKa = 4.57FVTSGGLVLKK224 pKa = 10.57QKK226 pKa = 9.61TPGVMKK232 pKa = 10.13SGCVNTISDD241 pKa = 3.81NSIMQFILHH250 pKa = 6.36ARR252 pKa = 11.84VCLEE256 pKa = 4.13LGLPVTPMFSMGDD269 pKa = 3.39DD270 pKa = 3.2RR271 pKa = 11.84LQVPVRR277 pKa = 11.84DD278 pKa = 3.59QKK280 pKa = 11.68SYY282 pKa = 11.66VGLTSQFCILKK293 pKa = 10.23SVQLKK298 pKa = 10.71NEE300 pKa = 3.7FAGFEE305 pKa = 4.14FRR307 pKa = 11.84GSVVDD312 pKa = 4.13PVHH315 pKa = 6.61KK316 pKa = 10.42GKK318 pKa = 9.62HH319 pKa = 5.07AFNILHH325 pKa = 6.57MDD327 pKa = 3.87DD328 pKa = 4.3EE329 pKa = 4.97VAEE332 pKa = 4.46SMANSYY338 pKa = 9.32VLNYY342 pKa = 9.48HH343 pKa = 7.0RR344 pKa = 11.84SPWRR348 pKa = 11.84NWMEE352 pKa = 3.72RR353 pKa = 11.84LFNGMGIEE361 pKa = 4.7LIPRR365 pKa = 11.84TARR368 pKa = 11.84DD369 pKa = 3.57VIFDD373 pKa = 3.92GFF375 pKa = 3.56

Molecular weight: 42.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE52|A0A1L3KE52_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 26 OX=1923212 PE=4 SV=1
MM1 pKa = 7.0NTTANTNNSEE11 pKa = 4.15GFQGEE16 pKa = 4.18PGGMFRR22 pKa = 11.84VRR24 pKa = 11.84LLGEE28 pKa = 4.12EE29 pKa = 4.03PVEE32 pKa = 3.79PVYY35 pKa = 10.79RR36 pKa = 11.84PTSEE40 pKa = 4.15EE41 pKa = 3.8VGEE44 pKa = 4.51SILTKK49 pKa = 10.49ALRR52 pKa = 11.84KK53 pKa = 9.26LAFWTGLMVLAALSIVSLLVIVYY76 pKa = 9.43RR77 pKa = 11.84WLAHH81 pKa = 5.79GAACVRR87 pKa = 11.84RR88 pKa = 11.84AVFSQFSYY96 pKa = 9.58MYY98 pKa = 9.43RR99 pKa = 11.84WLDD102 pKa = 3.49EE103 pKa = 4.3FSPSQTEE110 pKa = 3.79WEE112 pKa = 4.17RR113 pKa = 11.84AEE115 pKa = 4.03KK116 pKa = 10.51EE117 pKa = 3.67RR118 pKa = 11.84TLRR121 pKa = 11.84EE122 pKa = 3.7RR123 pKa = 11.84FGEE126 pKa = 4.13MWSQAVGFYY135 pKa = 10.52SVPLFLSGMDD145 pKa = 3.44WIDD148 pKa = 3.82WILLLAVVILTLIGLRR164 pKa = 11.84LAARR168 pKa = 11.84GCFSSGARR176 pKa = 11.84IVRR179 pKa = 11.84SWRR182 pKa = 11.84GVHH185 pKa = 5.36YY186 pKa = 10.24EE187 pKa = 3.59AMRR190 pKa = 11.84PGSFFVKK197 pKa = 10.36GDD199 pKa = 3.54VPDD202 pKa = 4.35CQVSVMLPGLLSDD215 pKa = 3.53SHH217 pKa = 7.34QGYY220 pKa = 9.42GIRR223 pKa = 11.84YY224 pKa = 8.21GEE226 pKa = 3.99YY227 pKa = 10.0LIVPRR232 pKa = 11.84HH233 pKa = 4.99VVAGHH238 pKa = 5.52QEE240 pKa = 4.45LILSSKK246 pKa = 8.64RR247 pKa = 11.84AKK249 pKa = 10.11VAVNVTFAQSRR260 pKa = 11.84LNEE263 pKa = 4.42DD264 pKa = 3.22LAYY267 pKa = 10.02MYY269 pKa = 10.55VGQNAFSQLGAVNAKK284 pKa = 9.72FPKK287 pKa = 10.38KK288 pKa = 10.43FMSTYY293 pKa = 8.69ATCTGLPGAVSARR306 pKa = 11.84VAKK309 pKa = 10.54SIIRR313 pKa = 11.84GKK315 pKa = 10.7LVFDD319 pKa = 4.83GSTLPGMSGAAYY331 pKa = 7.69MAQGLVLGIHH341 pKa = 5.94QGQSGMINVGLSADD355 pKa = 4.18LFRR358 pKa = 11.84AEE360 pKa = 3.49IKK362 pKa = 10.66YY363 pKa = 10.29LVRR366 pKa = 11.84QEE368 pKa = 4.59SVTGHH373 pKa = 6.1YY374 pKa = 10.68DD375 pKa = 3.05RR376 pKa = 11.84TDD378 pKa = 3.3DD379 pKa = 4.52DD380 pKa = 4.53RR381 pKa = 11.84PSKK384 pKa = 10.52FQANWNYY391 pKa = 10.8EE392 pKa = 4.36EE393 pKa = 4.18IEE395 pKa = 4.21KK396 pKa = 10.29MSEE399 pKa = 3.47LRR401 pKa = 11.84YY402 pKa = 10.44SQDD405 pKa = 2.46DD406 pKa = 3.67WAFDD410 pKa = 3.48AAIDD414 pKa = 4.22YY415 pKa = 9.97EE416 pKa = 4.25EE417 pKa = 4.2QLDD420 pKa = 3.99FGEE423 pKa = 4.54SMVRR427 pKa = 11.84KK428 pKa = 8.61PGPRR432 pKa = 11.84VRR434 pKa = 11.84LVPDD438 pKa = 3.05VEE440 pKa = 4.14GALRR444 pKa = 11.84VEE446 pKa = 4.53VAGQNTDD453 pKa = 3.08GKK455 pKa = 10.42SVSFDD460 pKa = 3.02AVQSHH465 pKa = 6.6HH466 pKa = 7.25LDD468 pKa = 3.28FLDD471 pKa = 3.47TLRR474 pKa = 11.84AANVLEE480 pKa = 4.63RR481 pKa = 11.84IEE483 pKa = 4.37ALEE486 pKa = 3.88SRR488 pKa = 11.84IKK490 pKa = 10.55PKK492 pKa = 10.53EE493 pKa = 3.52QVAPKK498 pKa = 9.94EE499 pKa = 4.18SAPPAFPCSEE509 pKa = 3.85CRR511 pKa = 11.84ARR513 pKa = 11.84CHH515 pKa = 6.1TEE517 pKa = 3.61EE518 pKa = 4.15KK519 pKa = 10.24LANHH523 pKa = 5.73MMVHH527 pKa = 5.72KK528 pKa = 10.25QYY530 pKa = 10.72PCEE533 pKa = 4.25SCDD536 pKa = 3.83KK537 pKa = 10.9VFTGEE542 pKa = 3.85AKK544 pKa = 10.63LARR547 pKa = 11.84HH548 pKa = 6.2LRR550 pKa = 11.84SAHH553 pKa = 5.61PVQPEE558 pKa = 4.05SAVPEE563 pKa = 4.15DD564 pKa = 3.44TGKK567 pKa = 10.45SGRR570 pKa = 11.84QVKK573 pKa = 8.35MEE575 pKa = 4.17KK576 pKa = 10.56VPFLGKK582 pKa = 10.09KK583 pKa = 10.15ASNQNRR589 pKa = 11.84KK590 pKa = 9.67KK591 pKa = 10.68SLSLNSSASAGKK603 pKa = 10.01KK604 pKa = 9.68GSPSVEE610 pKa = 3.52ASQCLISEE618 pKa = 4.54FQKK621 pKa = 11.14SMRR624 pKa = 11.84VLLKK628 pKa = 10.7EE629 pKa = 4.02FAEE632 pKa = 4.44TLAGQNSATMRR643 pKa = 11.84NN644 pKa = 3.63

Molecular weight: 71.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1019

375

644

509.5

57.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.243 ± 0.831

1.668 ± 0.105

4.809 ± 0.839

6.379 ± 0.832

4.711 ± 0.328

6.968 ± 0.44

2.159 ± 0.014

4.024 ± 0.69

5.005 ± 0.249

8.93 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.435 ± 0.298

3.337 ± 0.35

4.514 ± 0.151

4.22 ± 0.025

6.771 ± 0.055

8.243 ± 0.55

3.729 ± 0.143

7.949 ± 0.254

2.257 ± 0.637

2.65 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski