Plantactinospora sp. CNZ320
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7098 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N3S8S3|A0A2N3S8S3_9ACTN Dihydrofolate reductase OS=Plantactinospora sp. CNZ320 OX=2035246 GN=CLT71_3021 PE=4 SV=1
MM1 pKa = 7.12 TPIRR5 pKa = 11.84 LVLDD9 pKa = 3.79 ATAITAYY16 pKa = 9.82 AATSIAVGEE25 pKa = 4.57 VISEE29 pKa = 4.16 VAEE32 pKa = 3.84 EE33 pKa = 3.85 NAYY36 pKa = 10.69 VGLPLGCLVTAYY48 pKa = 10.06 RR49 pKa = 11.84 QASEE53 pKa = 4.5 DD54 pKa = 3.58 QLPQIGVLVDD64 pKa = 3.54 HH65 pKa = 7.1 PSVVIVGTDD74 pKa = 3.36 PADD77 pKa = 3.21 WLAFAGLARR86 pKa = 11.84 DD87 pKa = 4.31 LGRR90 pKa = 11.84 DD91 pKa = 3.25 AAEE94 pKa = 4.21 ALLLAVDD101 pKa = 3.43 SDD103 pKa = 4.84 GYY105 pKa = 11.71 VLTSDD110 pKa = 3.65 PGSYY114 pKa = 10.84 GNPDD118 pKa = 4.69 DD119 pKa = 5.98 LPVIPII125 pKa = 4.07
Molecular weight: 12.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.656
IPC_protein 3.617
Toseland 3.401
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.745
Patrickios 0.769
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A2N3SD18|A0A2N3SD18_9ACTN RNA polymerase ECF family sigma subunit OS=Plantactinospora sp. CNZ320 OX=2035246 GN=CLT71_4542 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIIAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.67 GRR40 pKa = 11.84 TRR42 pKa = 11.84 LSAA45 pKa = 3.71
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7098
0
7098
2359260
29
5479
332.4
35.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.552 ± 0.039
0.752 ± 0.008
5.93 ± 0.024
5.214 ± 0.027
2.619 ± 0.016
9.591 ± 0.03
2.019 ± 0.014
3.253 ± 0.02
1.483 ± 0.018
10.541 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.582 ± 0.01
1.85 ± 0.018
6.542 ± 0.027
2.763 ± 0.016
8.717 ± 0.033
4.888 ± 0.021
6.058 ± 0.027
8.911 ± 0.027
1.602 ± 0.012
2.132 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here