Streptococcus satellite phage Javan754

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZVV8|A0A4D5ZVV8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan754 OX=2558849 GN=JavanS754_0017 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.85NKK4 pKa = 10.68LKK6 pKa = 10.38EE7 pKa = 3.86LRR9 pKa = 11.84LNKK12 pKa = 9.77EE13 pKa = 4.12FNGKK17 pKa = 9.08IGMTQQEE24 pKa = 4.29IADD27 pKa = 4.39EE28 pKa = 4.27IGVTKK33 pKa = 9.86RR34 pKa = 11.84TYY36 pKa = 9.94IYY38 pKa = 8.97WEE40 pKa = 3.89KK41 pKa = 11.12GEE43 pKa = 4.19RR44 pKa = 11.84QIKK47 pKa = 8.83PEE49 pKa = 4.04KK50 pKa = 9.89AQQLADD56 pKa = 3.26IFGVSVGQLLGYY68 pKa = 10.15EE69 pKa = 4.32DD70 pKa = 4.9YY71 pKa = 9.92KK72 pKa = 11.07TIQNDD77 pKa = 3.13AFDD80 pKa = 4.38SYY82 pKa = 11.93RR83 pKa = 11.84NMAKK87 pKa = 10.67LLRR90 pKa = 11.84TNPDD94 pKa = 2.94FKK96 pKa = 11.36NIISEE101 pKa = 4.03YY102 pKa = 11.04DD103 pKa = 2.99EE104 pKa = 4.35TNRR107 pKa = 11.84KK108 pKa = 8.63NGKK111 pKa = 9.27LDD113 pKa = 3.46LSFFVQAEE121 pKa = 4.17SLPIIEE127 pKa = 4.46QDD129 pKa = 2.91IKK131 pKa = 11.35EE132 pKa = 4.68LILEE136 pKa = 4.38EE137 pKa = 4.26WEE139 pKa = 4.17KK140 pKa = 9.36TQPEE144 pKa = 4.14DD145 pKa = 4.24DD146 pKa = 3.94YY147 pKa = 11.94EE148 pKa = 4.65EE149 pKa = 4.93IGGTLSDD156 pKa = 4.33NISRR160 pKa = 11.84IYY162 pKa = 9.82IALGQLPILFKK173 pKa = 11.28DD174 pKa = 3.98FFGSFLTLSTSDD186 pKa = 3.69KK187 pKa = 10.33KK188 pKa = 11.02IVMQLVNSLYY198 pKa = 10.69EE199 pKa = 3.91KK200 pKa = 10.84NKK202 pKa = 10.55DD203 pKa = 2.82IGIIKK208 pKa = 10.22EE209 pKa = 4.2YY210 pKa = 9.7TDD212 pKa = 3.36KK213 pKa = 11.23KK214 pKa = 10.87

Molecular weight:
24.89 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZWP8|A0A4D5ZWP8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan754 OX=2558849 GN=JavanS754_0018 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITEE5 pKa = 4.15YY6 pKa = 10.16TKK8 pKa = 10.79KK9 pKa = 10.58DD10 pKa = 2.93GSTVYY15 pKa = 10.37RR16 pKa = 11.84SSVYY20 pKa = 10.68LGIDD24 pKa = 3.15TVTGKK29 pKa = 10.24KK30 pKa = 10.27VKK32 pKa = 8.07TTISGRR38 pKa = 11.84TKK40 pKa = 10.61RR41 pKa = 11.84EE42 pKa = 3.76LKK44 pKa = 10.7AKK46 pKa = 10.16ALQAQIDD53 pKa = 4.0FEE55 pKa = 5.43KK56 pKa = 11.01DD57 pKa = 2.64GSTVYY62 pKa = 10.61KK63 pKa = 10.4AVEE66 pKa = 3.74IKK68 pKa = 9.8TYY70 pKa = 11.23AEE72 pKa = 4.37LVEE75 pKa = 4.62NWLEE79 pKa = 4.57TYY81 pKa = 10.53CHH83 pKa = 5.45TVKK86 pKa = 10.81KK87 pKa = 9.8ATLTIIKK94 pKa = 10.16SRR96 pKa = 11.84LNNYY100 pKa = 8.42LLPAFGNYY108 pKa = 9.89RR109 pKa = 11.84LDD111 pKa = 3.53KK112 pKa = 9.67LTPPVIQKK120 pKa = 9.14QVNQWAKK127 pKa = 10.6EE128 pKa = 3.84YY129 pKa = 9.72NQLGKK134 pKa = 10.69GYY136 pKa = 9.89QEE138 pKa = 3.99YY139 pKa = 9.14PQLNSLNKK147 pKa = 10.15RR148 pKa = 11.84ILKK151 pKa = 9.76YY152 pKa = 10.38AVSLQVIPFNPARR165 pKa = 11.84DD166 pKa = 3.53IIVPRR171 pKa = 11.84RR172 pKa = 11.84KK173 pKa = 10.05AKK175 pKa = 10.24EE176 pKa = 4.02GQKK179 pKa = 10.55LKK181 pKa = 11.17YY182 pKa = 10.43LDD184 pKa = 4.8DD185 pKa = 5.74DD186 pKa = 4.04NLKK189 pKa = 10.76KK190 pKa = 10.49FLNYY194 pKa = 10.31LEE196 pKa = 4.5QLPNTYY202 pKa = 10.74KK203 pKa = 10.78NFYY206 pKa = 9.48DD207 pKa = 3.51TVLYY211 pKa = 8.15KK212 pKa = 10.45TLLATGLRR220 pKa = 11.84IRR222 pKa = 11.84EE223 pKa = 4.08CLALKK228 pKa = 10.05WSDD231 pKa = 3.08IDD233 pKa = 4.02LQNGTLDD240 pKa = 3.42VNKK243 pKa = 9.41TLNCEE248 pKa = 4.2KK249 pKa = 10.44EE250 pKa = 4.29VTSPKK255 pKa = 8.61TKK257 pKa = 10.49SSIRR261 pKa = 11.84VIDD264 pKa = 4.28LDD266 pKa = 3.81NKK268 pKa = 8.09TVLMLRR274 pKa = 11.84LYY276 pKa = 10.7KK277 pKa = 10.34NRR279 pKa = 11.84QAQVGRR285 pKa = 11.84EE286 pKa = 3.53IGLTYY291 pKa = 10.68EE292 pKa = 4.41KK293 pKa = 10.78VFSNSFDD300 pKa = 3.44EE301 pKa = 4.33YY302 pKa = 10.78RR303 pKa = 11.84DD304 pKa = 3.44ARR306 pKa = 11.84ALRR309 pKa = 11.84SRR311 pKa = 11.84LEE313 pKa = 3.85KK314 pKa = 10.32HH315 pKa = 6.43LKK317 pKa = 10.66LSDD320 pKa = 3.61CPRR323 pKa = 11.84LTFHH327 pKa = 7.8AFRR330 pKa = 11.84HH331 pKa = 4.54THH333 pKa = 6.71ASILLNAGLPYY344 pKa = 10.43KK345 pKa = 10.2EE346 pKa = 3.68IQTRR350 pKa = 11.84LGHH353 pKa = 6.71AKK355 pKa = 10.54LSMTMDD361 pKa = 3.71IYY363 pKa = 11.79SHH365 pKa = 7.09LSKK368 pKa = 10.88DD369 pKa = 3.45NKK371 pKa = 10.49KK372 pKa = 10.17NATSFYY378 pKa = 9.85EE379 pKa = 3.99KK380 pKa = 10.33AIEE383 pKa = 4.04KK384 pKa = 10.35LKK386 pKa = 11.16SSS388 pKa = 3.82

Molecular weight:
44.92 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3441

49

388

163.9

18.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.26 ± 0.523

0.785 ± 0.094

6.219 ± 0.342

8.079 ± 0.625

4.33 ± 0.361

4.417 ± 0.412

1.511 ± 0.254

7.498 ± 0.448

10.375 ± 0.681

10.317 ± 0.513

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.234

6.074 ± 0.496

2.441 ± 0.195

3.72 ± 0.436

4.563 ± 0.337

6.277 ± 0.43

6.248 ± 0.39

4.679 ± 0.375

0.727 ± 0.133

4.534 ± 0.35

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski