Vibrio phage VP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Vapseptimavirus; unclassified Vapseptimavirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P2THA6|A0A4P2THA6_9CAUD Uncharacterized protein OS=Vibrio phage VP-1 OX=2234088 PE=4 SV=1
MM1 pKa = 6.94SQITFSEE8 pKa = 4.27RR9 pKa = 11.84TKK11 pKa = 10.8QILSNFSKK19 pKa = 10.41ISPSIFLHH27 pKa = 6.27EE28 pKa = 4.51GNILRR33 pKa = 11.84TLDD36 pKa = 3.38STGGVMAKK44 pKa = 10.33AVIEE48 pKa = 3.79EE49 pKa = 4.91DD50 pKa = 3.3IPVNFPVGNLKK61 pKa = 10.74GLLQTINLATFKK73 pKa = 10.64NSKK76 pKa = 10.01IEE78 pKa = 3.87MDD80 pKa = 3.96EE81 pKa = 4.78DD82 pKa = 4.09KK83 pKa = 8.93MTLIAGNSTLDD94 pKa = 3.48YY95 pKa = 10.62YY96 pKa = 11.17ATDD99 pKa = 3.61EE100 pKa = 4.86DD101 pKa = 4.53SVEE104 pKa = 4.34SIEE107 pKa = 5.72DD108 pKa = 3.75DD109 pKa = 3.85VEE111 pKa = 4.13PEE113 pKa = 3.5YY114 pKa = 11.11DD115 pKa = 3.23INFTFDD121 pKa = 2.99IDD123 pKa = 4.3NEE125 pKa = 4.57TFCDD129 pKa = 4.56FKK131 pKa = 11.4SAAKK135 pKa = 10.49GMGLPYY141 pKa = 10.76VKK143 pKa = 9.82IEE145 pKa = 4.34GKK147 pKa = 10.51DD148 pKa = 3.42KK149 pKa = 10.68VTMKK153 pKa = 10.74AFNPDD158 pKa = 3.73LPTSTVFTITLADD171 pKa = 3.91TFDD174 pKa = 3.7GTPFEE179 pKa = 5.0VEE181 pKa = 3.93MKK183 pKa = 10.16LSNMQSIIDD192 pKa = 3.53GNYY195 pKa = 7.17TVKK198 pKa = 10.39VCEE201 pKa = 3.8EE202 pKa = 3.84DD203 pKa = 3.86GYY205 pKa = 11.75NIFTAIDD212 pKa = 3.6GNLEE216 pKa = 4.04YY217 pKa = 10.19IIGHH221 pKa = 5.59EE222 pKa = 4.11EE223 pKa = 3.95SLL225 pKa = 3.94

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P2TH94|A0A4P2TH94_9CAUD Portal protein OS=Vibrio phage VP-1 OX=2234088 PE=3 SV=1
MM1 pKa = 7.71AKK3 pKa = 10.2GKK5 pKa = 8.6RR6 pKa = 11.84TNKK9 pKa = 9.57KK10 pKa = 9.92RR11 pKa = 11.84FVNEE15 pKa = 2.61NRR17 pKa = 11.84MAVRR21 pKa = 11.84WAGAQKK27 pKa = 10.32ILNFLSRR34 pKa = 11.84GTKK37 pKa = 9.31GRR39 pKa = 11.84VVAGDD44 pKa = 3.72PSAPQPLFRR53 pKa = 11.84LCEE56 pKa = 4.01MYY58 pKa = 11.04NLL60 pKa = 4.19

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

33182

48

1622

319.1

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.368 ± 0.171

1.034 ± 0.101

6.434 ± 0.141

7.136 ± 0.287

4.141 ± 0.132

6.847 ± 0.204

2.188 ± 0.123

6.299 ± 0.104

7.341 ± 0.213

7.477 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.108

5.572 ± 0.167

3.638 ± 0.112

3.788 ± 0.132

4.816 ± 0.137

5.816 ± 0.144

6.142 ± 0.251

6.69 ± 0.144

1.456 ± 0.091

3.894 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski