Amino acid dipepetide frequency for Vibrio phage VP-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.762AlaAla: 4.762 ± 0.415
0.663AlaCys: 0.663 ± 0.163
4.249AlaAsp: 4.249 ± 0.406
4.46AlaGlu: 4.46 ± 0.418
2.652AlaPhe: 2.652 ± 0.31
4.28AlaGly: 4.28 ± 0.511
1.055AlaHis: 1.055 ± 0.212
4.249AlaIle: 4.249 ± 0.357
4.973AlaLys: 4.973 ± 0.41
4.189AlaLeu: 4.189 ± 0.353
2.17AlaMet: 2.17 ± 0.237
3.345AlaAsn: 3.345 ± 0.363
2.381AlaPro: 2.381 ± 0.271
2.773AlaGln: 2.773 ± 0.334
3.044AlaArg: 3.044 ± 0.302
3.707AlaSer: 3.707 ± 0.296
3.586AlaThr: 3.586 ± 0.459
4.28AlaVal: 4.28 ± 0.409
0.874AlaTrp: 0.874 ± 0.163
1.989AlaTyr: 1.989 ± 0.236
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.161
0.211CysCys: 0.211 ± 0.079
0.904CysAsp: 0.904 ± 0.166
0.784CysGlu: 0.784 ± 0.16
0.271CysPhe: 0.271 ± 0.087
0.512CysGly: 0.512 ± 0.145
0.332CysHis: 0.332 ± 0.099
0.693CysIle: 0.693 ± 0.131
0.633CysLys: 0.633 ± 0.123
0.482CysLeu: 0.482 ± 0.14
0.241CysMet: 0.241 ± 0.087
0.693CysAsn: 0.693 ± 0.144
0.603CysPro: 0.603 ± 0.149
0.181CysGln: 0.181 ± 0.075
0.603CysArg: 0.603 ± 0.14
0.332CysSer: 0.332 ± 0.098
0.633CysThr: 0.633 ± 0.179
0.814CysVal: 0.814 ± 0.181
0.121CysTrp: 0.121 ± 0.055
0.512CysTyr: 0.512 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
3.918AspAla: 3.918 ± 0.322
0.964AspCys: 0.964 ± 0.191
3.556AspAsp: 3.556 ± 0.413
4.581AspGlu: 4.581 ± 0.435
2.803AspPhe: 2.803 ± 0.278
5.575AspGly: 5.575 ± 0.501
1.175AspHis: 1.175 ± 0.215
4.732AspIle: 4.732 ± 0.359
4.46AspLys: 4.46 ± 0.384
4.46AspLeu: 4.46 ± 0.312
2.26AspMet: 2.26 ± 0.31
3.707AspAsn: 3.707 ± 0.308
2.351AspPro: 2.351 ± 0.274
1.989AspGln: 1.989 ± 0.233
2.23AspArg: 2.23 ± 0.236
3.647AspSer: 3.647 ± 0.295
3.436AspThr: 3.436 ± 0.305
4.4AspVal: 4.4 ± 0.398
1.296AspTrp: 1.296 ± 0.193
2.803AspTyr: 2.803 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
4.912GluAla: 4.912 ± 0.447
0.753GluCys: 0.753 ± 0.165
4.46GluAsp: 4.46 ± 0.443
5.154GluGlu: 5.154 ± 0.502
3.496GluPhe: 3.496 ± 0.375
3.888GluGly: 3.888 ± 0.404
2.019GluHis: 2.019 ± 0.214
4.943GluIle: 4.943 ± 0.377
4.943GluLys: 4.943 ± 0.445
5.786GluLeu: 5.786 ± 0.424
1.869GluMet: 1.869 ± 0.225
3.858GluAsn: 3.858 ± 0.267
2.411GluPro: 2.411 ± 0.241
3.586GluGln: 3.586 ± 0.36
3.074GluArg: 3.074 ± 0.366
4.159GluSer: 4.159 ± 0.397
4.038GluThr: 4.038 ± 0.324
3.858GluVal: 3.858 ± 0.406
1.115GluTrp: 1.115 ± 0.157
3.044GluTyr: 3.044 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
2.2PheAla: 2.2 ± 0.244
0.271PheCys: 0.271 ± 0.102
3.406PheAsp: 3.406 ± 0.314
3.586PheGlu: 3.586 ± 0.407
1.567PhePhe: 1.567 ± 0.236
2.411PheGly: 2.411 ± 0.269
1.055PheHis: 1.055 ± 0.163
2.23PheIle: 2.23 ± 0.211
3.164PheLys: 3.164 ± 0.351
2.321PheLeu: 2.321 ± 0.305
1.296PheMet: 1.296 ± 0.188
2.652PheAsn: 2.652 ± 0.319
1.115PhePro: 1.115 ± 0.148
1.537PheGln: 1.537 ± 0.246
2.11PheArg: 2.11 ± 0.249
2.17PheSer: 2.17 ± 0.254
3.044PheThr: 3.044 ± 0.325
3.014PheVal: 3.014 ± 0.272
0.603PheTrp: 0.603 ± 0.167
1.658PheTyr: 1.658 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
3.104GlyAla: 3.104 ± 0.318
0.482GlyCys: 0.482 ± 0.159
4.4GlyAsp: 4.4 ± 0.299
4.641GlyGlu: 4.641 ± 0.455
2.984GlyPhe: 2.984 ± 0.293
4.973GlyGly: 4.973 ± 0.492
1.808GlyHis: 1.808 ± 0.258
4.521GlyIle: 4.521 ± 0.413
5.093GlyLys: 5.093 ± 0.476
5.274GlyLeu: 5.274 ± 0.34
1.838GlyMet: 1.838 ± 0.269
3.586GlyAsn: 3.586 ± 0.364
1.627GlyPro: 1.627 ± 0.219
2.712GlyGln: 2.712 ± 0.282
3.617GlyArg: 3.617 ± 0.385
4.641GlySer: 4.641 ± 0.462
4.732GlyThr: 4.732 ± 0.569
5.003GlyVal: 5.003 ± 0.373
1.025GlyTrp: 1.025 ± 0.148
2.411GlyTyr: 2.411 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.236HisAla: 1.236 ± 0.202
0.241HisCys: 0.241 ± 0.085
0.995HisAsp: 0.995 ± 0.164
1.567HisGlu: 1.567 ± 0.212
1.025HisPhe: 1.025 ± 0.158
1.477HisGly: 1.477 ± 0.234
0.663HisHis: 0.663 ± 0.144
1.477HisIle: 1.477 ± 0.226
1.929HisLys: 1.929 ± 0.255
1.597HisLeu: 1.597 ± 0.25
0.633HisMet: 0.633 ± 0.148
1.025HisAsn: 1.025 ± 0.167
0.874HisPro: 0.874 ± 0.154
0.784HisGln: 0.784 ± 0.187
1.236HisArg: 1.236 ± 0.168
1.025HisSer: 1.025 ± 0.179
1.296HisThr: 1.296 ± 0.215
1.658HisVal: 1.658 ± 0.229
0.151HisTrp: 0.151 ± 0.066
0.995HisTyr: 0.995 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.43IleAla: 4.43 ± 0.343
0.633IleCys: 0.633 ± 0.167
4.491IleAsp: 4.491 ± 0.289
5.244IleGlu: 5.244 ± 0.386
2.14IlePhe: 2.14 ± 0.255
4.34IleGly: 4.34 ± 0.384
1.055IleHis: 1.055 ± 0.158
2.923IleIle: 2.923 ± 0.287
5.515IleLys: 5.515 ± 0.365
4.159IleLeu: 4.159 ± 0.377
1.748IleMet: 1.748 ± 0.235
3.647IleAsn: 3.647 ± 0.343
3.074IlePro: 3.074 ± 0.319
2.923IleGln: 2.923 ± 0.282
3.014IleArg: 3.014 ± 0.271
3.737IleSer: 3.737 ± 0.384
3.556IleThr: 3.556 ± 0.327
3.225IleVal: 3.225 ± 0.361
0.753IleTrp: 0.753 ± 0.157
2.381IleTyr: 2.381 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
5.666LysAla: 5.666 ± 0.472
0.753LysCys: 0.753 ± 0.196
4.46LysAsp: 4.46 ± 0.416
4.611LysGlu: 4.611 ± 0.397
2.743LysPhe: 2.743 ± 0.257
4.852LysGly: 4.852 ± 0.534
2.08LysHis: 2.08 ± 0.303
3.677LysIle: 3.677 ± 0.317
4.641LysLys: 4.641 ± 0.568
6.721LysLeu: 6.721 ± 0.43
2.17LysMet: 2.17 ± 0.267
3.797LysAsn: 3.797 ± 0.354
3.074LysPro: 3.074 ± 0.359
2.773LysGln: 2.773 ± 0.26
4.219LysArg: 4.219 ± 0.337
3.466LysSer: 3.466 ± 0.426
4.249LysThr: 4.249 ± 0.324
5.244LysVal: 5.244 ± 0.415
1.236LysTrp: 1.236 ± 0.166
2.984LysTyr: 2.984 ± 0.358
0.0LysXaa: 0.0 ± 0.0
Leu
5.033LeuAla: 5.033 ± 0.39
0.723LeuCys: 0.723 ± 0.179
4.882LeuAsp: 4.882 ± 0.41
6.329LeuGlu: 6.329 ± 0.405
3.225LeuPhe: 3.225 ± 0.364
4.099LeuGly: 4.099 ± 0.312
1.597LeuHis: 1.597 ± 0.191
4.551LeuIle: 4.551 ± 0.383
5.756LeuLys: 5.756 ± 0.429
5.575LeuLeu: 5.575 ± 0.495
2.351LeuMet: 2.351 ± 0.267
4.4LeuAsn: 4.4 ± 0.398
2.863LeuPro: 2.863 ± 0.311
2.411LeuGln: 2.411 ± 0.204
3.225LeuArg: 3.225 ± 0.32
4.4LeuSer: 4.4 ± 0.368
4.159LeuThr: 4.159 ± 0.329
4.852LeuVal: 4.852 ± 0.455
0.844LeuTrp: 0.844 ± 0.201
3.496LeuTyr: 3.496 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
2.743MetAla: 2.743 ± 0.324
0.362MetCys: 0.362 ± 0.102
1.627MetAsp: 1.627 ± 0.256
1.838MetGlu: 1.838 ± 0.223
1.236MetPhe: 1.236 ± 0.244
1.658MetGly: 1.658 ± 0.233
0.512MetHis: 0.512 ± 0.107
1.567MetIle: 1.567 ± 0.2
2.08MetLys: 2.08 ± 0.246
2.049MetLeu: 2.049 ± 0.276
0.995MetMet: 0.995 ± 0.191
1.658MetAsn: 1.658 ± 0.217
1.175MetPro: 1.175 ± 0.216
0.995MetGln: 0.995 ± 0.16
1.688MetArg: 1.688 ± 0.279
2.019MetSer: 2.019 ± 0.225
1.658MetThr: 1.658 ± 0.205
1.869MetVal: 1.869 ± 0.263
0.211MetTrp: 0.211 ± 0.077
1.266MetTyr: 1.266 ± 0.222
0.0MetXaa: 0.0 ± 0.0
Asn
3.345AsnAla: 3.345 ± 0.356
0.512AsnCys: 0.512 ± 0.128
3.134AsnAsp: 3.134 ± 0.3
3.586AsnGlu: 3.586 ± 0.296
2.381AsnPhe: 2.381 ± 0.287
5.003AsnGly: 5.003 ± 0.445
1.055AsnHis: 1.055 ± 0.198
3.797AsnIle: 3.797 ± 0.359
3.586AsnLys: 3.586 ± 0.327
3.888AsnLeu: 3.888 ± 0.389
1.688AsnMet: 1.688 ± 0.24
3.345AsnAsn: 3.345 ± 0.366
2.23AsnPro: 2.23 ± 0.265
1.838AsnGln: 1.838 ± 0.205
2.863AsnArg: 2.863 ± 0.29
3.195AsnSer: 3.195 ± 0.316
3.737AsnThr: 3.737 ± 0.445
3.888AsnVal: 3.888 ± 0.426
0.633AsnTrp: 0.633 ± 0.147
2.019AsnTyr: 2.019 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
1.597ProAla: 1.597 ± 0.227
0.332ProCys: 0.332 ± 0.1
2.351ProAsp: 2.351 ± 0.227
3.074ProGlu: 3.074 ± 0.309
1.537ProPhe: 1.537 ± 0.248
2.682ProGly: 2.682 ± 0.266
0.633ProHis: 0.633 ± 0.143
2.26ProIle: 2.26 ± 0.284
2.501ProLys: 2.501 ± 0.276
2.26ProLeu: 2.26 ± 0.314
0.934ProMet: 0.934 ± 0.163
2.321ProAsn: 2.321 ± 0.289
1.145ProPro: 1.145 ± 0.198
0.693ProGln: 0.693 ± 0.17
1.718ProArg: 1.718 ± 0.249
2.652ProSer: 2.652 ± 0.332
2.953ProThr: 2.953 ± 0.334
2.501ProVal: 2.501 ± 0.294
0.663ProTrp: 0.663 ± 0.15
1.567ProTyr: 1.567 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
2.532GlnAla: 2.532 ± 0.284
0.422GlnCys: 0.422 ± 0.115
2.08GlnAsp: 2.08 ± 0.227
2.682GlnGlu: 2.682 ± 0.329
1.929GlnPhe: 1.929 ± 0.226
2.23GlnGly: 2.23 ± 0.263
0.904GlnHis: 0.904 ± 0.156
2.773GlnIle: 2.773 ± 0.337
2.682GlnLys: 2.682 ± 0.261
2.984GlnLeu: 2.984 ± 0.207
1.145GlnMet: 1.145 ± 0.172
1.808GlnAsn: 1.808 ± 0.189
1.447GlnPro: 1.447 ± 0.249
1.658GlnGln: 1.658 ± 0.222
1.658GlnArg: 1.658 ± 0.193
2.11GlnSer: 2.11 ± 0.282
2.411GlnThr: 2.411 ± 0.293
2.682GlnVal: 2.682 ± 0.35
0.512GlnTrp: 0.512 ± 0.151
1.236GlnTyr: 1.236 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
2.321ArgAla: 2.321 ± 0.286
0.603ArgCys: 0.603 ± 0.151
2.863ArgAsp: 2.863 ± 0.292
3.345ArgGlu: 3.345 ± 0.427
2.019ArgPhe: 2.019 ± 0.27
3.285ArgGly: 3.285 ± 0.237
0.874ArgHis: 0.874 ± 0.192
3.586ArgIle: 3.586 ± 0.285
3.586ArgLys: 3.586 ± 0.315
3.888ArgLeu: 3.888 ± 0.334
1.236ArgMet: 1.236 ± 0.193
2.381ArgAsn: 2.381 ± 0.252
1.447ArgPro: 1.447 ± 0.181
2.11ArgGln: 2.11 ± 0.242
2.953ArgArg: 2.953 ± 0.254
2.381ArgSer: 2.381 ± 0.227
2.803ArgThr: 2.803 ± 0.398
3.285ArgVal: 3.285 ± 0.305
1.175ArgTrp: 1.175 ± 0.15
2.019ArgTyr: 2.019 ± 0.259
0.0ArgXaa: 0.0 ± 0.0
Ser
3.888SerAla: 3.888 ± 0.334
0.422SerCys: 0.422 ± 0.109
3.104SerAsp: 3.104 ± 0.321
3.466SerGlu: 3.466 ± 0.334
2.23SerPhe: 2.23 ± 0.237
4.822SerGly: 4.822 ± 0.513
1.206SerHis: 1.206 ± 0.202
3.948SerIle: 3.948 ± 0.386
4.732SerLys: 4.732 ± 0.323
4.822SerLeu: 4.822 ± 0.365
1.447SerMet: 1.447 ± 0.225
3.466SerAsn: 3.466 ± 0.313
1.989SerPro: 1.989 ± 0.233
2.08SerGln: 2.08 ± 0.304
2.411SerArg: 2.411 ± 0.281
3.436SerSer: 3.436 ± 0.296
3.797SerThr: 3.797 ± 0.354
3.948SerVal: 3.948 ± 0.335
0.723SerTrp: 0.723 ± 0.167
2.23SerTyr: 2.23 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
3.406ThrAla: 3.406 ± 0.434
0.663ThrCys: 0.663 ± 0.171
3.858ThrAsp: 3.858 ± 0.361
4.069ThrGlu: 4.069 ± 0.367
2.833ThrPhe: 2.833 ± 0.308
4.581ThrGly: 4.581 ± 0.599
1.206ThrHis: 1.206 ± 0.241
3.496ThrIle: 3.496 ± 0.355
4.43ThrLys: 4.43 ± 0.389
5.937ThrLeu: 5.937 ± 0.397
1.658ThrMet: 1.658 ± 0.216
3.255ThrAsn: 3.255 ± 0.272
2.712ThrPro: 2.712 ± 0.375
1.688ThrGln: 1.688 ± 0.227
2.321ThrArg: 2.321 ± 0.248
4.038ThrSer: 4.038 ± 0.543
3.918ThrThr: 3.918 ± 0.476
4.43ThrVal: 4.43 ± 0.424
0.964ThrTrp: 0.964 ± 0.201
1.959ThrTyr: 1.959 ± 0.25
0.0ThrXaa: 0.0 ± 0.0
Val
3.797ValAla: 3.797 ± 0.318
0.663ValCys: 0.663 ± 0.129
5.425ValAsp: 5.425 ± 0.412
5.154ValGlu: 5.154 ± 0.409
2.2ValPhe: 2.2 ± 0.248
4.099ValGly: 4.099 ± 0.399
1.326ValHis: 1.326 ± 0.211
4.43ValIle: 4.43 ± 0.363
4.762ValLys: 4.762 ± 0.366
4.37ValLeu: 4.37 ± 0.365
1.899ValMet: 1.899 ± 0.264
4.008ValAsn: 4.008 ± 0.294
2.351ValPro: 2.351 ± 0.232
2.863ValGln: 2.863 ± 0.314
3.014ValArg: 3.014 ± 0.294
4.219ValSer: 4.219 ± 0.329
4.34ValThr: 4.34 ± 0.432
4.641ValVal: 4.641 ± 0.357
0.844ValTrp: 0.844 ± 0.153
2.501ValTyr: 2.501 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
0.934TrpAla: 0.934 ± 0.172
0.271TrpCys: 0.271 ± 0.087
1.206TrpAsp: 1.206 ± 0.2
0.995TrpGlu: 0.995 ± 0.175
0.633TrpPhe: 0.633 ± 0.157
0.844TrpGly: 0.844 ± 0.147
0.422TrpHis: 0.422 ± 0.135
0.904TrpIle: 0.904 ± 0.212
1.055TrpLys: 1.055 ± 0.176
1.296TrpLeu: 1.296 ± 0.217
0.452TrpMet: 0.452 ± 0.122
0.693TrpAsn: 0.693 ± 0.143
0.362TrpPro: 0.362 ± 0.114
0.693TrpGln: 0.693 ± 0.151
0.693TrpArg: 0.693 ± 0.16
0.964TrpSer: 0.964 ± 0.176
0.542TrpThr: 0.542 ± 0.135
0.995TrpVal: 0.995 ± 0.174
0.241TrpTrp: 0.241 ± 0.096
0.362TrpTyr: 0.362 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.074TyrAla: 3.074 ± 0.308
0.392TyrCys: 0.392 ± 0.11
2.893TyrAsp: 2.893 ± 0.335
2.2TyrGlu: 2.2 ± 0.266
1.507TyrPhe: 1.507 ± 0.221
2.863TyrGly: 2.863 ± 0.282
0.904TyrHis: 0.904 ± 0.162
2.23TyrIle: 2.23 ± 0.277
2.893TyrLys: 2.893 ± 0.357
2.712TyrLeu: 2.712 ± 0.355
1.175TyrMet: 1.175 ± 0.169
2.08TyrAsn: 2.08 ± 0.246
1.145TyrPro: 1.145 ± 0.17
1.597TyrGln: 1.597 ± 0.231
2.471TyrArg: 2.471 ± 0.252
1.869TyrSer: 1.869 ± 0.268
2.532TyrThr: 2.532 ± 0.314
2.321TyrVal: 2.321 ± 0.272
0.573TyrTrp: 0.573 ± 0.166
1.507TyrTyr: 1.507 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (33182 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski