Dishui Lake large algae virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA viruses

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XPX9|A0A6G6XPX9_9VIRU Uncharacterized protein OS=Dishui Lake large algae virus 1 OX=2704073 PE=4 SV=1
MM1 pKa = 7.65DD2 pKa = 3.5TFMPVRR8 pKa = 11.84FRR10 pKa = 11.84TQVQLAPNNISVNIDD25 pKa = 2.93EE26 pKa = 5.94RR27 pKa = 11.84ITEE30 pKa = 4.38KK31 pKa = 10.53IKK33 pKa = 11.35SNLEE37 pKa = 4.36GICSRR42 pKa = 11.84FGYY45 pKa = 9.7IRR47 pKa = 11.84PGSIEE52 pKa = 3.62IVKK55 pKa = 10.46RR56 pKa = 11.84SVGCLMKK63 pKa = 10.67SHH65 pKa = 6.92FNGYY69 pKa = 8.64IKK71 pKa = 10.88YY72 pKa = 10.55DD73 pKa = 3.68IVCKK77 pKa = 10.55ADD79 pKa = 2.88VCNPVRR85 pKa = 11.84GMIFKK90 pKa = 10.66VIVKK94 pKa = 9.82NKK96 pKa = 10.09NEE98 pKa = 4.1LGILAEE104 pKa = 4.19GSIVIDD110 pKa = 3.9GNNLSVLDD118 pKa = 3.59VLIPRR123 pKa = 11.84RR124 pKa = 11.84SAGITSDD131 pKa = 3.2IDD133 pKa = 4.1LDD135 pKa = 4.11TVDD138 pKa = 4.13VGDD141 pKa = 4.33EE142 pKa = 3.99IFTEE146 pKa = 4.48VLGKK150 pKa = 10.31RR151 pKa = 11.84YY152 pKa = 10.18SLNDD156 pKa = 3.11KK157 pKa = 10.0RR158 pKa = 11.84ISIIARR164 pKa = 11.84AVKK167 pKa = 9.89EE168 pKa = 4.07PSKK171 pKa = 10.86KK172 pKa = 9.81RR173 pKa = 11.84YY174 pKa = 8.88KK175 pKa = 10.92DD176 pKa = 3.15KK177 pKa = 11.2DD178 pKa = 3.39VEE180 pKa = 4.4KK181 pKa = 10.4PVTEE185 pKa = 3.91YY186 pKa = 11.42DD187 pKa = 3.46EE188 pKa = 5.34AVYY191 pKa = 8.65GTDD194 pKa = 3.84GRR196 pKa = 11.84IEE198 pKa = 5.0GIDD201 pKa = 3.37DD202 pKa = 3.86DD203 pKa = 5.31EE204 pKa = 5.23YY205 pKa = 11.03IGLDD209 pKa = 3.6GEE211 pKa = 4.35EE212 pKa = 5.3DD213 pKa = 3.54EE214 pKa = 5.76TDD216 pKa = 5.4DD217 pKa = 4.02EE218 pKa = 4.99DD219 pKa = 5.97KK220 pKa = 11.06SIKK223 pKa = 10.65DD224 pKa = 4.61GEE226 pKa = 4.78DD227 pKa = 3.51DD228 pKa = 5.77DD229 pKa = 6.43NDD231 pKa = 5.09DD232 pKa = 5.35EE233 pKa = 7.04DD234 pKa = 6.52DD235 pKa = 4.58EE236 pKa = 6.89DD237 pKa = 6.47DD238 pKa = 3.72EE239 pKa = 6.58DD240 pKa = 4.88EE241 pKa = 5.67EE242 pKa = 4.7EE243 pKa = 5.38DD244 pKa = 5.0HH245 pKa = 7.75DD246 pKa = 5.9DD247 pKa = 5.87DD248 pKa = 5.74EE249 pKa = 6.65NSDD252 pKa = 6.08DD253 pKa = 6.14DD254 pKa = 5.81DD255 pKa = 5.7QDD257 pKa = 5.9DD258 pKa = 5.37DD259 pKa = 5.35DD260 pKa = 6.45DD261 pKa = 4.51LVEE264 pKa = 4.42TGGDD268 pKa = 3.32QIDD271 pKa = 3.69YY272 pKa = 11.44DD273 pKa = 4.08EE274 pKa = 4.44FF275 pKa = 6.24

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XQN7|A0A6G6XQN7_9VIRU Uncharacterized protein OS=Dishui Lake large algae virus 1 OX=2704073 PE=4 SV=1
MM1 pKa = 6.82NTSSLTIQDD10 pKa = 3.32DD11 pKa = 3.68VQRR14 pKa = 11.84IKK16 pKa = 11.25NSDD19 pKa = 2.95KK20 pKa = 9.9TAYY23 pKa = 9.91RR24 pKa = 11.84SLPIMTKK31 pKa = 10.4YY32 pKa = 10.3EE33 pKa = 3.97FNQIIGLRR41 pKa = 11.84TIHH44 pKa = 7.13LARR47 pKa = 11.84GAIPFVDD54 pKa = 3.48VEE56 pKa = 4.42GGIEE60 pKa = 3.91RR61 pKa = 11.84NMNLRR66 pKa = 11.84AVALKK71 pKa = 9.84EE72 pKa = 4.0LKK74 pKa = 9.9EE75 pKa = 4.22GKK77 pKa = 9.82LPYY80 pKa = 9.45IVKK83 pKa = 10.31RR84 pKa = 11.84PMPNGKK90 pKa = 9.78AEE92 pKa = 3.89YY93 pKa = 9.1WKK95 pKa = 11.07ANNLSLISVRR105 pKa = 11.84HH106 pKa = 5.4LLRR109 pKa = 11.84PP110 pKa = 3.65

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

208

0

208

84399

63

3042

405.8

45.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.782 ± 0.185

1.616 ± 0.124

6.09 ± 0.161

5.566 ± 0.256

3.718 ± 0.105

6.215 ± 0.285

2.273 ± 0.1

7.498 ± 0.131

6.979 ± 0.338

7.761 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.128

5.818 ± 0.263

4.203 ± 0.132

3.213 ± 0.077

4.872 ± 0.195

6.888 ± 0.296

6.463 ± 0.364

6.37 ± 0.108

1.201 ± 0.048

4.581 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski