Cronobacter phage JC01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3YLN9|A0A6M3YLN9_9CAUD Uncharacterized protein OS=Cronobacter phage JC01 OX=2729575 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84AISLSEE8 pKa = 3.93SASYY12 pKa = 10.27RR13 pKa = 11.84VLYY16 pKa = 10.21TSLAHH21 pKa = 6.78VSPADD26 pKa = 3.69LANLKK31 pKa = 10.41SMCEE35 pKa = 3.39AGYY38 pKa = 10.49YY39 pKa = 9.37FVHH42 pKa = 6.35NVEE45 pKa = 4.58NDD47 pKa = 3.19FSGIICRR54 pKa = 11.84YY55 pKa = 9.08GAVTPEE61 pKa = 4.0SDD63 pKa = 3.4VGEE66 pKa = 4.3VMIGDD71 pKa = 3.87YY72 pKa = 10.94GFSEE76 pKa = 4.73SFGEE80 pKa = 4.53MIAQFKK86 pKa = 10.6DD87 pKa = 2.92AGFDD91 pKa = 3.39AVHH94 pKa = 6.73FDD96 pKa = 3.19VDD98 pKa = 3.35GDD100 pKa = 4.07LVEE103 pKa = 4.6GVTWYY108 pKa = 9.68TEE110 pKa = 3.73NNTRR114 pKa = 11.84VQPIAYY120 pKa = 9.1GCEE123 pKa = 3.45ILTWWDD129 pKa = 3.27LAVEE133 pKa = 4.34HH134 pKa = 6.28QLTATDD140 pKa = 4.21EE141 pKa = 4.3YY142 pKa = 11.33GDD144 pKa = 5.68DD145 pKa = 3.71GDD147 pKa = 5.81ADD149 pKa = 4.15LKK151 pKa = 11.35YY152 pKa = 10.65FLADD156 pKa = 3.35GVLYY160 pKa = 10.98CLDD163 pKa = 3.0NFMRR167 pKa = 11.84VTDD170 pKa = 4.29GEE172 pKa = 4.21MQEE175 pKa = 4.28MGFHH179 pKa = 6.73ASAGEE184 pKa = 4.34SNGSAICITLSDD196 pKa = 3.6NSDD199 pKa = 3.67FVDD202 pKa = 3.68VARR205 pKa = 11.84VSS207 pKa = 3.25

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3YKK5|A0A6M3YKK5_9CAUD Uncharacterized protein OS=Cronobacter phage JC01 OX=2729575 PE=4 SV=1
MM1 pKa = 7.28SVTVVTAGSKK11 pKa = 10.54EE12 pKa = 3.77LMTYY16 pKa = 10.82LEE18 pKa = 4.7TVPEE22 pKa = 4.03LAKK25 pKa = 10.38RR26 pKa = 11.84AMRR29 pKa = 11.84LAINGTAKK37 pKa = 10.53GKK39 pKa = 10.77GMTAIKK45 pKa = 10.14RR46 pKa = 11.84QMLDD50 pKa = 3.0EE51 pKa = 4.22VAFPSGYY58 pKa = 10.76LNADD62 pKa = 4.04RR63 pKa = 11.84LRR65 pKa = 11.84VTRR68 pKa = 11.84LASDD72 pKa = 3.71NNLEE76 pKa = 3.96ATITGRR82 pKa = 11.84KK83 pKa = 8.83RR84 pKa = 11.84ATSLARR90 pKa = 11.84FVTGNPVVGSDD101 pKa = 2.97KK102 pKa = 11.37GGGVSVRR109 pKa = 11.84VQRR112 pKa = 11.84GRR114 pKa = 11.84TTYY117 pKa = 10.59LKK119 pKa = 10.3QAFLVRR125 pKa = 11.84LKK127 pKa = 10.72RR128 pKa = 11.84GASLSEE134 pKa = 4.13DD135 pKa = 3.6NYY137 pKa = 11.38NLGLAVRR144 pKa = 11.84LKK146 pKa = 10.6PGEE149 pKa = 4.14SLTNKK154 pKa = 8.41RR155 pKa = 11.84TQHH158 pKa = 5.46QAWLVPNKK166 pKa = 9.82VALLYY171 pKa = 10.73GPSVDD176 pKa = 3.6QVFSGVIEE184 pKa = 4.04EE185 pKa = 4.23LRR187 pKa = 11.84PVIGQEE193 pKa = 4.06VASEE197 pKa = 4.22FHH199 pKa = 6.33RR200 pKa = 11.84QFARR204 pKa = 11.84LSKK207 pKa = 10.84

Molecular weight: 22.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

19143

34

1502

251.9

28.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.166 ± 0.463

0.993 ± 0.124

6.436 ± 0.328

6.07 ± 0.312

3.662 ± 0.159

7.141 ± 0.221

1.651 ± 0.154

4.858 ± 0.165

5.13 ± 0.303

7.825 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.144

3.949 ± 0.172

4.801 ± 0.282

4.221 ± 0.33

6.143 ± 0.215

5.762 ± 0.278

5.94 ± 0.305

7.104 ± 0.251

1.849 ± 0.157

3.333 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski