Arthrobacter sp. (strain FB24)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4453 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0JY88|A0JY88_ARTS2 Neutral metalloproteinase OS=Arthrobacter sp. (strain FB24) OX=290399 GN=Arth_2629 PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84ALIIVDD9 pKa = 3.67VQNDD13 pKa = 3.63FCEE16 pKa = 4.89GGSLAVKK23 pKa = 10.5GGADD27 pKa = 3.23VAGAISEE34 pKa = 4.4YY35 pKa = 11.16VDD37 pKa = 3.43ACHH40 pKa = 6.47GQFDD44 pKa = 5.32HH45 pKa = 7.52IVATQDD51 pKa = 2.77WHH53 pKa = 7.24IEE55 pKa = 4.0PGAHH59 pKa = 6.58FSEE62 pKa = 5.33APDD65 pKa = 5.93FIDD68 pKa = 3.66SWPPHH73 pKa = 6.12CVAGTPGAEE82 pKa = 4.06LHH84 pKa = 7.01PDD86 pKa = 3.48LDD88 pKa = 3.77TEE90 pKa = 4.48YY91 pKa = 10.14IQAYY95 pKa = 7.9FRR97 pKa = 11.84KK98 pKa = 9.51GQFTAAYY105 pKa = 9.18SGFEE109 pKa = 4.42GILAPEE115 pKa = 4.66DD116 pKa = 3.67EE117 pKa = 4.8VPTGEE122 pKa = 4.57RR123 pKa = 11.84KK124 pKa = 9.62PGALPLPDD132 pKa = 4.48DD133 pKa = 4.57AEE135 pKa = 4.31EE136 pKa = 3.87LTAASFADD144 pKa = 3.89EE145 pKa = 4.51DD146 pKa = 5.75AIGLDD151 pKa = 4.3DD152 pKa = 4.21WLQSHH157 pKa = 6.64DD158 pKa = 3.86VEE160 pKa = 4.72DD161 pKa = 3.96VVIVGIATDD170 pKa = 3.89YY171 pKa = 11.37CVMATALDD179 pKa = 4.0AVQAGYY185 pKa = 10.75SVTVVRR191 pKa = 11.84NLTAGIAADD200 pKa = 4.3LDD202 pKa = 3.9DD203 pKa = 5.59AFAEE207 pKa = 4.31MEE209 pKa = 4.42LGGVDD214 pKa = 4.07LAA216 pKa = 5.14

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0K038|A0K038_ARTS2 Glyoxalase_6 domain-containing protein OS=Arthrobacter sp. (strain FB24) OX=290399 GN=Arth_3282 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.65KK12 pKa = 10.17IPGAQIVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 9.03KK31 pKa = 8.56NPRR34 pKa = 11.84MKK36 pKa = 10.47ARR38 pKa = 11.84QGG40 pKa = 3.24

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4453

0

4453

1486714

32

3524

333.9

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.168 ± 0.052

0.638 ± 0.009

5.565 ± 0.028

5.53 ± 0.033

3.247 ± 0.021

9.209 ± 0.032

2.067 ± 0.017

4.324 ± 0.028

2.837 ± 0.028

10.134 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.016

2.476 ± 0.023

5.552 ± 0.026

3.078 ± 0.021

6.504 ± 0.034

5.953 ± 0.026

5.875 ± 0.029

8.268 ± 0.031

1.459 ± 0.013

2.088 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski