Amino acid dipepetide frequency for Arthrobacter sp. (strain FB24)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.461AlaAla: 21.461 ± 0.204	0.863AlaCys: 0.863 ± 0.025	7.25AlaAsp: 7.25 ± 0.079	8.0AlaGlu: 8.0 ± 0.093	3.743AlaPhe: 3.743 ± 0.051	13.802AlaGly: 13.802 ± 0.116	2.214AlaHis: 2.214 ± 0.037	4.938AlaIle: 4.938 ± 0.07	3.693AlaLys: 3.693 ± 0.067	12.936AlaLeu: 12.936 ± 0.123	2.832AlaMet: 2.832 ± 0.043	2.67AlaAsn: 2.67 ± 0.042	6.342AlaPro: 6.342 ± 0.101	3.675AlaGln: 3.675 ± 0.059	7.747AlaArg: 7.747 ± 0.09	7.202AlaSer: 7.202 ± 0.075	6.765AlaThr: 6.765 ± 0.07	11.568AlaVal: 11.568 ± 0.105	1.669AlaTrp: 1.669 ± 0.041	2.306AlaTyr: 2.306 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	0.754CysAla: 0.754 ± 0.024	0.065CysCys: 0.065 ± 0.007	0.356CysAsp: 0.356 ± 0.014	0.309CysGlu: 0.309 ± 0.014	0.195CysPhe: 0.195 ± 0.011	0.735CysGly: 0.735 ± 0.021	0.139CysHis: 0.139 ± 0.01	0.256CysIle: 0.256 ± 0.014	0.126CysLys: 0.126 ± 0.008	0.596CysLeu: 0.596 ± 0.021	0.107CysMet: 0.107 ± 0.008	0.143CysAsn: 0.143 ± 0.01	0.39CysPro: 0.39 ± 0.018	0.17CysGln: 0.17 ± 0.011	0.456CysArg: 0.456 ± 0.019	0.436CysSer: 0.436 ± 0.016	0.435CysThr: 0.435 ± 0.018	0.46CysVal: 0.46 ± 0.017	0.109CysTrp: 0.109 ± 0.008	0.143CysTyr: 0.143 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.262AspAla: 7.262 ± 0.076	0.321AspCys: 0.321 ± 0.015	2.982AspAsp: 2.982 ± 0.053	3.29AspGlu: 3.29 ± 0.049	1.949AspPhe: 1.949 ± 0.037	5.795AspGly: 5.795 ± 0.068	1.238AspHis: 1.238 ± 0.028	2.44AspIle: 2.44 ± 0.04	1.456AspLys: 1.456 ± 0.032	5.265AspLeu: 5.265 ± 0.062	0.944AspMet: 0.944 ± 0.026	1.155AspAsn: 1.155 ± 0.029	3.734AspPro: 3.734 ± 0.051	1.64AspGln: 1.64 ± 0.037	3.451AspArg: 3.451 ± 0.058	2.939AspSer: 2.939 ± 0.047	2.75AspThr: 2.75 ± 0.047	4.885AspVal: 4.885 ± 0.072	0.856AspTrp: 0.856 ± 0.025	1.297AspTyr: 1.297 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	7.167GluAla: 7.167 ± 0.091	0.311GluCys: 0.311 ± 0.015	2.954GluAsp: 2.954 ± 0.044	3.211GluGlu: 3.211 ± 0.059	1.718GluPhe: 1.718 ± 0.036	4.022GluGly: 4.022 ± 0.055	1.395GluHis: 1.395 ± 0.032	2.522GluIle: 2.522 ± 0.042	1.748GluLys: 1.748 ± 0.032	6.854GluLeu: 6.854 ± 0.08	0.977GluMet: 0.977 ± 0.026	1.455GluAsn: 1.455 ± 0.03	2.916GluPro: 2.916 ± 0.048	2.236GluGln: 2.236 ± 0.043	3.946GluArg: 3.946 ± 0.062	2.978GluSer: 2.978 ± 0.045	2.939GluThr: 2.939 ± 0.051	3.956GluVal: 3.956 ± 0.055	0.867GluTrp: 0.867 ± 0.025	1.128GluTyr: 1.128 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.047PheAla: 4.047 ± 0.061	0.249PheCys: 0.249 ± 0.013	2.138PheAsp: 2.138 ± 0.033	1.671PheGlu: 1.671 ± 0.032	1.201PhePhe: 1.201 ± 0.033	3.408PheGly: 3.408 ± 0.058	0.676PheHis: 0.676 ± 0.022	1.438PheIle: 1.438 ± 0.035	0.831PheLys: 0.831 ± 0.026	3.031PheLeu: 3.031 ± 0.05	0.612PheMet: 0.612 ± 0.021	0.898PheAsn: 0.898 ± 0.021	1.526PhePro: 1.526 ± 0.035	0.877PheGln: 0.877 ± 0.024	1.904PheArg: 1.904 ± 0.036	2.042PheSer: 2.042 ± 0.045	2.162PheThr: 2.162 ± 0.041	2.522PheVal: 2.522 ± 0.042	0.486PheTrp: 0.486 ± 0.019	0.747PheTyr: 0.747 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.286GlyAla: 10.286 ± 0.097	0.666GlyCys: 0.666 ± 0.023	4.256GlyAsp: 4.256 ± 0.06	4.462GlyGlu: 4.462 ± 0.057	3.308GlyPhe: 3.308 ± 0.057	7.865GlyGly: 7.865 ± 0.09	2.084GlyHis: 2.084 ± 0.042	4.751GlyIle: 4.751 ± 0.064	3.154GlyLys: 3.154 ± 0.054	9.342GlyLeu: 9.342 ± 0.098	2.158GlyMet: 2.158 ± 0.039	2.441GlyAsn: 2.441 ± 0.043	4.458GlyPro: 4.458 ± 0.062	3.148GlyGln: 3.148 ± 0.049	6.139GlyArg: 6.139 ± 0.071	6.05GlySer: 6.05 ± 0.063	6.294GlyThr: 6.294 ± 0.073	7.212GlyVal: 7.212 ± 0.078	1.669GlyTrp: 1.669 ± 0.033	2.345GlyTyr: 2.345 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.303HisAla: 2.303 ± 0.04	0.153HisCys: 0.153 ± 0.01	1.164HisAsp: 1.164 ± 0.023	1.179HisGlu: 1.179 ± 0.028	0.684HisPhe: 0.684 ± 0.02	2.07HisGly: 2.07 ± 0.041	0.632HisHis: 0.632 ± 0.025	0.825HisIle: 0.825 ± 0.023	0.445HisLys: 0.445 ± 0.014	2.022HisLeu: 2.022 ± 0.039	0.366HisMet: 0.366 ± 0.017	0.474HisAsn: 0.474 ± 0.017	1.478HisPro: 1.478 ± 0.032	0.634HisGln: 0.634 ± 0.022	1.557HisArg: 1.557 ± 0.038	1.231HisSer: 1.231 ± 0.026	1.042HisThr: 1.042 ± 0.029	1.608HisVal: 1.608 ± 0.032	0.326HisTrp: 0.326 ± 0.017	0.474HisTyr: 0.474 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.754IleAla: 5.754 ± 0.075	0.327IleCys: 0.327 ± 0.017	2.617IleAsp: 2.617 ± 0.04	2.358IleGlu: 2.358 ± 0.046	1.391IlePhe: 1.391 ± 0.036	4.128IleGly: 4.128 ± 0.063	0.844IleHis: 0.844 ± 0.023	1.858IleIle: 1.858 ± 0.037	1.134IleLys: 1.134 ± 0.031	3.902IleLeu: 3.902 ± 0.061	0.796IleMet: 0.796 ± 0.024	1.277IleAsn: 1.277 ± 0.033	2.422IlePro: 2.422 ± 0.033	1.172IleGln: 1.172 ± 0.03	2.801IleArg: 2.801 ± 0.051	2.768IleSer: 2.768 ± 0.04	2.75IleThr: 2.75 ± 0.043	3.574IleVal: 3.574 ± 0.06	0.506IleTrp: 0.506 ± 0.02	0.857IleTyr: 0.857 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.782LysAla: 3.782 ± 0.057	0.115LysCys: 0.115 ± 0.009	1.888LysAsp: 1.888 ± 0.039	1.537LysGlu: 1.537 ± 0.034	0.783LysPhe: 0.783 ± 0.02	2.14LysGly: 2.14 ± 0.044	0.618LysHis: 0.618 ± 0.018	1.349LysIle: 1.349 ± 0.034	1.065LysLys: 1.065 ± 0.033	2.696LysLeu: 2.696 ± 0.042	0.628LysMet: 0.628 ± 0.019	0.789LysAsn: 0.789 ± 0.025	1.721LysPro: 1.721 ± 0.038	0.865LysGln: 0.865 ± 0.028	1.645LysArg: 1.645 ± 0.032	1.63LysSer: 1.63 ± 0.036	1.7LysThr: 1.7 ± 0.036	2.417LysVal: 2.417 ± 0.044	0.341LysTrp: 0.341 ± 0.013	0.663LysTyr: 0.663 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.303LeuAla: 14.303 ± 0.132	0.655LeuCys: 0.655 ± 0.021	6.013LeuAsp: 6.013 ± 0.064	5.384LeuGlu: 5.384 ± 0.061	3.015LeuPhe: 3.015 ± 0.047	9.113LeuGly: 9.113 ± 0.094	1.997LeuHis: 1.997 ± 0.04	4.316LeuIle: 4.316 ± 0.067	2.787LeuLys: 2.787 ± 0.048	10.384LeuLeu: 10.384 ± 0.137	2.022LeuMet: 2.022 ± 0.042	2.555LeuAsn: 2.555 ± 0.042	5.762LeuPro: 5.762 ± 0.068	3.003LeuGln: 3.003 ± 0.044	6.807LeuArg: 6.807 ± 0.075	6.063LeuSer: 6.063 ± 0.064	6.0LeuThr: 6.0 ± 0.053	8.191LeuVal: 8.191 ± 0.087	1.263LeuTrp: 1.263 ± 0.035	1.71LeuTyr: 1.71 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.672MetAla: 2.672 ± 0.042	0.13MetCys: 0.13 ± 0.009	1.092MetAsp: 1.092 ± 0.029	0.914MetGlu: 0.914 ± 0.023	0.598MetPhe: 0.598 ± 0.019	1.674MetGly: 1.674 ± 0.035	0.346MetHis: 0.346 ± 0.017	0.896MetIle: 0.896 ± 0.025	0.667MetLys: 0.667 ± 0.02	1.925MetLeu: 1.925 ± 0.04	0.434MetMet: 0.434 ± 0.02	0.609MetAsn: 0.609 ± 0.021	1.145MetPro: 1.145 ± 0.026	0.595MetGln: 0.595 ± 0.023	1.187MetArg: 1.187 ± 0.028	1.525MetSer: 1.525 ± 0.034	1.638MetThr: 1.638 ± 0.033	1.638MetVal: 1.638 ± 0.035	0.225MetTrp: 0.225 ± 0.013	0.373MetTyr: 0.373 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.873AsnAla: 2.873 ± 0.044	0.162AsnCys: 0.162 ± 0.011	1.314AsnAsp: 1.314 ± 0.033	1.199AsnGlu: 1.199 ± 0.031	0.823AsnPhe: 0.823 ± 0.025	2.44AsnGly: 2.44 ± 0.053	0.532AsnHis: 0.532 ± 0.017	1.113AsnIle: 1.113 ± 0.024	0.66AsnLys: 0.66 ± 0.024	2.245AsnLeu: 2.245 ± 0.043	0.496AsnMet: 0.496 ± 0.017	0.738AsnAsn: 0.738 ± 0.024	1.904AsnPro: 1.904 ± 0.036	0.79AsnGln: 0.79 ± 0.022	1.625AsnArg: 1.625 ± 0.038	1.415AsnSer: 1.415 ± 0.032	1.42AsnThr: 1.42 ± 0.034	1.996AsnVal: 1.996 ± 0.039	0.399AsnTrp: 0.399 ± 0.017	0.614AsnTyr: 0.614 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	8.46ProAla: 8.46 ± 0.102	0.251ProCys: 0.251 ± 0.013	3.751ProAsp: 3.751 ± 0.059	4.029ProGlu: 4.029 ± 0.053	1.735ProPhe: 1.735 ± 0.038	5.999ProGly: 5.999 ± 0.071	1.08ProHis: 1.08 ± 0.028	1.619ProIle: 1.619 ± 0.033	1.417ProLys: 1.417 ± 0.032	4.994ProLeu: 4.994 ± 0.057	0.997ProMet: 0.997 ± 0.027	1.166ProAsn: 1.166 ± 0.032	2.47ProPro: 2.47 ± 0.052	1.668ProGln: 1.668 ± 0.039	3.0ProArg: 3.0 ± 0.051	3.255ProSer: 3.255 ± 0.055	2.666ProThr: 2.666 ± 0.046	5.043ProVal: 5.043 ± 0.065	0.852ProTrp: 0.852 ± 0.028	1.07ProTyr: 1.07 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.956GlnAla: 3.956 ± 0.064	0.16GlnCys: 0.16 ± 0.011	1.62GlnAsp: 1.62 ± 0.038	1.631GlnGlu: 1.631 ± 0.026	0.94GlnPhe: 0.94 ± 0.028	2.395GlnGly: 2.395 ± 0.04	0.725GlnHis: 0.725 ± 0.022	1.334GlnIle: 1.334 ± 0.033	0.862GlnLys: 0.862 ± 0.025	3.742GlnLeu: 3.742 ± 0.053	0.578GlnMet: 0.578 ± 0.022	0.737GlnAsn: 0.737 ± 0.023	1.829GlnPro: 1.829 ± 0.037	1.279GlnGln: 1.279 ± 0.03	2.261GlnArg: 2.261 ± 0.046	1.683GlnSer: 1.683 ± 0.037	1.517GlnThr: 1.517 ± 0.035	2.328GlnVal: 2.328 ± 0.043	0.497GlnTrp: 0.497 ± 0.021	0.706GlnTyr: 0.706 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	6.849ArgAla: 6.849 ± 0.074	0.39ArgCys: 0.39 ± 0.015	3.449ArgAsp: 3.449 ± 0.046	3.768ArgGlu: 3.768 ± 0.054	2.22ArgPhe: 2.22 ± 0.035	4.725ArgGly: 4.725 ± 0.066	1.567ArgHis: 1.567 ± 0.036	3.354ArgIle: 3.354 ± 0.051	2.045ArgLys: 2.045 ± 0.04	6.886ArgLeu: 6.886 ± 0.084	1.519ArgMet: 1.519 ± 0.03	1.821ArgAsn: 1.821 ± 0.032	3.592ArgPro: 3.592 ± 0.052	2.357ArgGln: 2.357 ± 0.044	5.462ArgArg: 5.462 ± 0.084	3.934ArgSer: 3.934 ± 0.057	4.03ArgThr: 4.03 ± 0.052	4.48ArgVal: 4.48 ± 0.067	1.063ArgTrp: 1.063 ± 0.027	1.531ArgTyr: 1.531 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	7.554SerAla: 7.554 ± 0.08	0.391SerCys: 0.391 ± 0.017	2.824SerAsp: 2.824 ± 0.046	2.862SerGlu: 2.862 ± 0.046	2.11SerPhe: 2.11 ± 0.04	6.368SerGly: 6.368 ± 0.064	1.157SerHis: 1.157 ± 0.033	2.427SerIle: 2.427 ± 0.044	1.591SerLys: 1.591 ± 0.031	5.859SerLeu: 5.859 ± 0.057	1.367SerMet: 1.367 ± 0.03	1.431SerAsn: 1.431 ± 0.034	3.463SerPro: 3.463 ± 0.051	1.7SerGln: 1.7 ± 0.035	3.939SerArg: 3.939 ± 0.054	3.714SerSer: 3.714 ± 0.05	3.641SerThr: 3.641 ± 0.053	4.782SerVal: 4.782 ± 0.06	0.96SerTrp: 0.96 ± 0.025	1.392SerTyr: 1.392 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	8.204ThrAla: 8.204 ± 0.076	0.315ThrCys: 0.315 ± 0.014	3.172ThrAsp: 3.172 ± 0.044	3.083ThrGlu: 3.083 ± 0.048	1.888ThrPhe: 1.888 ± 0.04	6.053ThrGly: 6.053 ± 0.06	1.106ThrHis: 1.106 ± 0.026	2.443ThrIle: 2.443 ± 0.038	1.515ThrLys: 1.515 ± 0.034	5.479ThrLeu: 5.479 ± 0.063	1.086ThrMet: 1.086 ± 0.025	1.305ThrAsn: 1.305 ± 0.036	3.739ThrPro: 3.739 ± 0.056	1.439ThrGln: 1.439 ± 0.033	3.155ThrArg: 3.155 ± 0.049	3.454ThrSer: 3.454 ± 0.049	3.644ThrThr: 3.644 ± 0.067	5.704ThrVal: 5.704 ± 0.068	0.787ThrTrp: 0.787 ± 0.027	1.181ThrTyr: 1.181 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.21ValAla: 10.21 ± 0.098	0.584ValCys: 0.584 ± 0.023	4.818ValAsp: 4.818 ± 0.058	4.639ValGlu: 4.639 ± 0.065	2.763ValPhe: 2.763 ± 0.045	6.204ValGly: 6.204 ± 0.065	1.639ValHis: 1.639 ± 0.035	3.902ValIle: 3.902 ± 0.059	2.138ValLys: 2.138 ± 0.042	9.205ValLeu: 9.205 ± 0.09	1.68ValMet: 1.68 ± 0.039	2.037ValAsn: 2.037 ± 0.04	4.803ValPro: 4.803 ± 0.059	2.275ValGln: 2.275 ± 0.045	5.308ValArg: 5.308 ± 0.062	5.032ValSer: 5.032 ± 0.064	5.284ValThr: 5.284 ± 0.072	7.631ValVal: 7.631 ± 0.08	1.045ValTrp: 1.045 ± 0.027	1.487ValTyr: 1.487 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.523TrpAla: 1.523 ± 0.036	0.117TrpCys: 0.117 ± 0.009	0.782TrpAsp: 0.782 ± 0.023	0.651TrpGlu: 0.651 ± 0.023	0.576TrpPhe: 0.576 ± 0.022	1.039TrpGly: 1.039 ± 0.027	0.333TrpHis: 0.333 ± 0.016	0.696TrpIle: 0.696 ± 0.021	0.475TrpLys: 0.475 ± 0.018	1.782TrpLeu: 1.782 ± 0.043	0.334TrpMet: 0.334 ± 0.014	0.488TrpAsn: 0.488 ± 0.017	0.712TrpPro: 0.712 ± 0.024	0.607TrpGln: 0.607 ± 0.018	1.025TrpArg: 1.025 ± 0.028	0.868TrpSer: 0.868 ± 0.023	0.899TrpThr: 0.899 ± 0.027	1.047TrpVal: 1.047 ± 0.029	0.327TrpTrp: 0.327 ± 0.016	0.309TrpTyr: 0.309 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.259TyrAla: 2.259 ± 0.042	0.155TyrCys: 0.155 ± 0.01	1.208TyrAsp: 1.208 ± 0.03	1.123TyrGlu: 1.123 ± 0.029	0.827TyrPhe: 0.827 ± 0.026	2.113TyrGly: 2.113 ± 0.037	0.344TyrHis: 0.344 ± 0.016	0.763TyrIle: 0.763 ± 0.022	0.561TyrLys: 0.561 ± 0.024	2.197TyrLeu: 2.197 ± 0.039	0.344TyrMet: 0.344 ± 0.015	0.568TyrAsn: 0.568 ± 0.025	1.119TyrPro: 1.119 ± 0.028	0.649TyrGln: 0.649 ± 0.02	1.627TyrArg: 1.627 ± 0.035	1.312TyrSer: 1.312 ± 0.031	1.178TyrThr: 1.178 ± 0.026	1.641TyrVal: 1.641 ± 0.031	0.344TyrTrp: 0.344 ± 0.016	0.552TyrTyr: 0.552 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4453 proteins (1486714 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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