Agrobacterium phage 7-7-1
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7F9G2|J7F9G2_9CAUD Putative membrane protein OS=Agrobacterium phage 7-7-1 OX=1161931 GN=7-7-1_000110 PE=4 SV=1
MM1 pKa = 6.59 VTLVCAKK8 pKa = 10.24 PPEE11 pKa = 4.73 CPNVPVPLPPSPPAPAPIGGDD32 pKa = 2.65 IVVEE36 pKa = 4.03 NSDD39 pKa = 4.09 TIILSGSGTTDD50 pKa = 2.79 SPLRR54 pKa = 11.84 AEE56 pKa = 4.24 LAFPLRR62 pKa = 11.84 IDD64 pKa = 4.21 LASTTYY70 pKa = 11.09 DD71 pKa = 3.24 EE72 pKa = 4.99 LLSGEE77 pKa = 4.19 LQMVYY82 pKa = 9.39 TAVEE86 pKa = 4.04 TFSFVNAEE94 pKa = 4.46 CEE96 pKa = 4.34 GSVNPAIVTTLTVSVNDD113 pKa = 4.22 APIGDD118 pKa = 3.82 ISIASNQFSFDD129 pKa = 3.74 LNEE132 pKa = 4.23 AAVITPGDD140 pKa = 3.71 VVKK143 pKa = 9.84 ITTTEE148 pKa = 3.75 ALIFDD153 pKa = 4.43 YY154 pKa = 11.08 LAITLVGNVTPP165 pKa = 5.0
Molecular weight: 17.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 3.579
IPC_protein 3.516
Toseland 3.325
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.452
Rodwell 3.363
Grimsley 3.236
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.821
Thurlkill 3.389
EMBOSS 3.465
Sillero 3.643
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.646
Protein with the highest isoelectric point:
>tr|J7FAQ1|J7FAQ1_9CAUD Uncharacterized protein OS=Agrobacterium phage 7-7-1 OX=1161931 GN=7-7-1_0005 PE=4 SV=1
MM1 pKa = 7.48 QINFKK6 pKa = 9.95 PWGRR10 pKa = 11.84 FSAFKK15 pKa = 10.22 DD16 pKa = 3.1 QRR18 pKa = 11.84 QIRR21 pKa = 11.84 RR22 pKa = 11.84 FLSDD26 pKa = 2.84 SADD29 pKa = 3.17 IVLKK33 pKa = 10.44 RR34 pKa = 11.84 LKK36 pKa = 10.59 DD37 pKa = 3.63 GLRR40 pKa = 11.84 NPPKK44 pKa = 10.31 SGRR47 pKa = 11.84 LYY49 pKa = 10.16 FGKK52 pKa = 10.1 SKK54 pKa = 9.76 RR55 pKa = 11.84 TIRR58 pKa = 11.84 ASVNRR63 pKa = 11.84 ALAEE67 pKa = 4.0 YY68 pKa = 8.13 PARR71 pKa = 11.84 DD72 pKa = 3.25 TGDD75 pKa = 3.43 LFRR78 pKa = 11.84 SAKK81 pKa = 9.83 KK82 pKa = 10.09 RR83 pKa = 11.84 VGRR86 pKa = 11.84 DD87 pKa = 3.12 EE88 pKa = 4.55 MEE90 pKa = 3.9 VGTNMFYY97 pKa = 11.01 SRR99 pKa = 11.84 FLRR102 pKa = 11.84 EE103 pKa = 4.33 GTSKK107 pKa = 9.63 MARR110 pKa = 11.84 RR111 pKa = 11.84 KK112 pKa = 9.62 MSDD115 pKa = 2.87 TALEE119 pKa = 4.01 EE120 pKa = 4.38 SLPKK124 pKa = 10.23 LDD126 pKa = 3.72 GKK128 pKa = 10.35 IGRR131 pKa = 11.84 FAYY134 pKa = 8.86 WKK136 pKa = 10.51 RR137 pKa = 11.84 NN138 pKa = 3.35
Molecular weight: 16.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.897
IPC_protein 10.906
Toseland 11.14
ProMoST 11.125
Dawson 11.184
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.359
Grimsley 11.228
Solomon 11.447
Lehninger 11.389
Nozaki 11.125
DTASelect 10.979
Thurlkill 11.125
EMBOSS 11.579
Sillero 11.14
Patrickios 11.082
IPC_peptide 11.447
IPC2_peptide 10.028
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
127
0
127
21814
28
781
171.8
19.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.086 ± 0.331
1.449 ± 0.162
5.808 ± 0.21
6.455 ± 0.231
4.465 ± 0.161
7.555 ± 0.302
1.692 ± 0.125
5.813 ± 0.158
5.286 ± 0.278
7.046 ± 0.162
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.113
4.548 ± 0.179
5.075 ± 0.215
3.007 ± 0.147
6.184 ± 0.247
5.996 ± 0.206
6.083 ± 0.226
7.486 ± 0.237
1.449 ± 0.095
3.058 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here