Brachybacterium muris UCD-AY4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium; Brachybacterium muris

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A022L0R3|A0A022L0R3_9MICO Amidophosphoribosyltransferase OS=Brachybacterium muris UCD-AY4 OX=1249481 GN=purF PE=3 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84LGSTPRR8 pKa = 11.84RR9 pKa = 11.84SPAHH13 pKa = 6.35RR14 pKa = 11.84PPTRR18 pKa = 11.84RR19 pKa = 11.84PLARR23 pKa = 11.84LSAVAAAAVLVVAGCVPQEE42 pKa = 4.01GQGPGEE48 pKa = 4.3TPSTGQSDD56 pKa = 3.44NGGAGSGDD64 pKa = 3.37GTTDD68 pKa = 3.07GTSGDD73 pKa = 3.95GQEE76 pKa = 4.43LATLGTSAAKK86 pKa = 10.16DD87 pKa = 3.83LPTDD91 pKa = 3.72PAEE94 pKa = 4.61DD95 pKa = 3.46PAYY98 pKa = 10.32AAYY101 pKa = 10.42YY102 pKa = 9.0EE103 pKa = 4.54QDD105 pKa = 3.77ITWGPCDD112 pKa = 3.77DD113 pKa = 4.12VEE115 pKa = 5.26GGSEE119 pKa = 4.37LEE121 pKa = 4.64CGTLTVPMVWNDD133 pKa = 3.65PGAGDD138 pKa = 3.82IDD140 pKa = 4.3LAVARR145 pKa = 11.84HH146 pKa = 5.36AASGNRR152 pKa = 11.84TGSLILNPGGPGGSGVDD169 pKa = 4.42FTASAPFLFSRR180 pKa = 11.84PVVDD184 pKa = 5.06AYY186 pKa = 10.93DD187 pKa = 3.8IIGFDD192 pKa = 3.47PRR194 pKa = 11.84GVARR198 pKa = 11.84SAGIQCLSDD207 pKa = 3.72EE208 pKa = 4.48EE209 pKa = 4.31TDD211 pKa = 3.48EE212 pKa = 4.23YY213 pKa = 11.54LAGTGDD219 pKa = 4.1MGTEE223 pKa = 4.06DD224 pKa = 4.18PLEE227 pKa = 4.5EE228 pKa = 4.0GLAWMKK234 pKa = 10.63RR235 pKa = 11.84IAEE238 pKa = 4.46GCQQDD243 pKa = 3.62SGEE246 pKa = 4.42LLPYY250 pKa = 10.41LDD252 pKa = 4.52TYY254 pKa = 11.15SAARR258 pKa = 11.84DD259 pKa = 3.61MDD261 pKa = 4.06VLRR264 pKa = 11.84AAVDD268 pKa = 3.88SEE270 pKa = 3.72QLDD273 pKa = 4.0YY274 pKa = 11.21FGYY277 pKa = 10.47SYY279 pKa = 10.34GTYY282 pKa = 10.46LGATYY287 pKa = 11.09ADD289 pKa = 5.2LYY291 pKa = 9.42PEE293 pKa = 4.15RR294 pKa = 11.84VGKK297 pKa = 9.92FVLDD301 pKa = 3.77AALDD305 pKa = 3.93PSSTLDD311 pKa = 3.85EE312 pKa = 4.56ISAGQAEE319 pKa = 4.79GFEE322 pKa = 4.4LATDD326 pKa = 4.52AFLEE330 pKa = 4.52DD331 pKa = 4.97CLAQGDD337 pKa = 4.18SCPFKK342 pKa = 11.21GEE344 pKa = 3.83PAEE347 pKa = 5.56ANQQLQNFFDD357 pKa = 4.47SIEE360 pKa = 4.16AQPLEE365 pKa = 4.31TSDD368 pKa = 3.49PQRR371 pKa = 11.84PLTVSLAQSAVLLLMYY387 pKa = 10.38EE388 pKa = 4.27DD389 pKa = 5.17GLWQVGRR396 pKa = 11.84DD397 pKa = 3.53ALAQGMQGDD406 pKa = 5.06GSALLQIADD415 pKa = 4.5LSSEE419 pKa = 4.09RR420 pKa = 11.84QPDD423 pKa = 3.55GSYY426 pKa = 10.45KK427 pKa = 10.23GNGMFAINAINCLDD441 pKa = 3.75HH442 pKa = 7.39PGIADD447 pKa = 3.68QEE449 pKa = 4.42WQAKK453 pKa = 7.11EE454 pKa = 4.06AEE456 pKa = 4.3RR457 pKa = 11.84LAAEE461 pKa = 4.48YY462 pKa = 7.54PTFGPSMGYY471 pKa = 9.89GATMCAQWPVPPLRR485 pKa = 11.84EE486 pKa = 3.95PAPISAEE493 pKa = 3.85GAGPIVVIGTTGDD506 pKa = 3.32PATPYY511 pKa = 10.19RR512 pKa = 11.84WSQSLAEE519 pKa = 4.14QLDD522 pKa = 4.0DD523 pKa = 4.8AVLLTFEE530 pKa = 5.2GNGHH534 pKa = 4.96TAYY537 pKa = 10.14GRR539 pKa = 11.84SGGCIEE545 pKa = 4.53EE546 pKa = 4.85AVDD549 pKa = 4.27AYY551 pKa = 10.98LLEE554 pKa = 4.65GTTPQDD560 pKa = 3.55GLTCSSGGG568 pKa = 3.46

Molecular weight: 59.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A022L020|A0A022L020_9MICO Uncharacterized protein OS=Brachybacterium muris UCD-AY4 OX=1249481 GN=D641_0109990 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIINARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84SEE42 pKa = 4.37LSAA45 pKa = 4.77

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2806

0

2806

943634

37

2201

336.3

36.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.726 ± 0.06

0.558 ± 0.011

6.393 ± 0.04

6.283 ± 0.038

2.686 ± 0.03

9.121 ± 0.043

2.27 ± 0.024

4.129 ± 0.035

1.869 ± 0.032

10.271 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.018

1.7 ± 0.024

5.799 ± 0.035

3.218 ± 0.023

7.578 ± 0.054

5.529 ± 0.028

6.22 ± 0.033

8.447 ± 0.048

1.387 ± 0.019

1.788 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski