Trichococcus palustris
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2699 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A143YA43|A0A143YA43_9LACT ATP-dependent helicase/nuclease subunit A OS=Trichococcus palustris OX=140314 GN=addA PE=3 SV=1
MM1 pKa = 8.1 IYY3 pKa = 10.46 EE4 pKa = 4.42 FRR6 pKa = 11.84 LSLRR10 pKa = 11.84 DD11 pKa = 2.87 IGIPVWRR18 pKa = 11.84 DD19 pKa = 3.06 IQVDD23 pKa = 4.08 CTDD26 pKa = 3.9 SFHH29 pKa = 7.93 DD30 pKa = 3.99 FNTYY34 pKa = 9.1 IQALFDD40 pKa = 3.35 WSSIFEE46 pKa = 4.05 HH47 pKa = 6.71 RR48 pKa = 11.84 FEE50 pKa = 4.08 ITRR53 pKa = 11.84 SGGVEE58 pKa = 3.88 IPPVIIEE65 pKa = 4.12 EE66 pKa = 4.31 TSMDD70 pKa = 3.61 EE71 pKa = 4.63 YY72 pKa = 11.17 EE73 pKa = 4.77 ALDD76 pKa = 3.89 EE77 pKa = 4.92 EE78 pKa = 5.77 GDD80 pKa = 3.73 FADD83 pKa = 5.45 AFSEE87 pKa = 4.71 DD88 pKa = 4.12 SFDD91 pKa = 3.88 EE92 pKa = 4.56 NYY94 pKa = 10.71 EE95 pKa = 3.91 LDD97 pKa = 3.71 YY98 pKa = 11.82 EE99 pKa = 4.37 EE100 pKa = 6.18 AFDD103 pKa = 3.92 EE104 pKa = 5.16 DD105 pKa = 4.57 EE106 pKa = 4.02 YY107 pKa = 11.64 RR108 pKa = 11.84 PIGSYY113 pKa = 10.36 FVDD116 pKa = 3.48 VGDD119 pKa = 3.77 QATYY123 pKa = 9.54 TYY125 pKa = 9.39 FTDD128 pKa = 3.49 AVLEE132 pKa = 4.01 IEE134 pKa = 5.04 IILTEE139 pKa = 4.01 IVAAQEE145 pKa = 3.87 DD146 pKa = 4.25 ATYY149 pKa = 9.75 PLCTGAEE156 pKa = 3.94 NLAPEE161 pKa = 4.38 EE162 pKa = 3.88 MDD164 pKa = 3.91 DD165 pKa = 5.81 RR166 pKa = 11.84 GDD168 pKa = 3.68 ILQKK172 pKa = 9.82 KK173 pKa = 9.57 AKK175 pKa = 9.52 LISVKK180 pKa = 10.55 KK181 pKa = 9.23 EE182 pKa = 3.61 EE183 pKa = 4.47 LVAAINKK190 pKa = 8.6 EE191 pKa = 3.82 LALLGDD197 pKa = 5.03 AISYY201 pKa = 9.96 DD202 pKa = 3.45 SEE204 pKa = 4.6 YY205 pKa = 11.3 EE206 pKa = 4.07 EE207 pKa = 4.64 FQHH210 pKa = 7.19 DD211 pKa = 3.86 LHH213 pKa = 8.31 FDD215 pKa = 3.48 DD216 pKa = 6.44 DD217 pKa = 4.74 EE218 pKa = 6.6 IYY220 pKa = 10.83 DD221 pKa = 3.9 EE222 pKa = 5.34 PYY224 pKa = 10.68 DD225 pKa = 4.29 AEE227 pKa = 4.87 SDD229 pKa = 3.97 NSDD232 pKa = 3.55 CC233 pKa = 5.85
Molecular weight: 26.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.745
DTASelect 3.948
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.77
Patrickios 0.769
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A143YKT4|A0A143YKT4_9LACT Phosphate-specific transport system accessory protein PhoU OS=Trichococcus palustris OX=140314 GN=Tpal_1395 PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.69 RR13 pKa = 11.84 FKK15 pKa = 10.09 RR16 pKa = 11.84 TGNGGLKK23 pKa = 9.55 RR24 pKa = 11.84 WSAFTSHH31 pKa = 6.95 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.4 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 QASMVSPSDD54 pKa = 3.3 MKK56 pKa = 10.83 RR57 pKa = 11.84 ISQQLSQMRR66 pKa = 3.6
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.046
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2699
0
2699
820627
26
2918
304.0
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.128 ± 0.051
0.718 ± 0.013
5.504 ± 0.034
7.185 ± 0.051
4.45 ± 0.036
7.011 ± 0.043
1.871 ± 0.02
7.6 ± 0.044
6.592 ± 0.035
9.542 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.866 ± 0.022
4.415 ± 0.034
3.509 ± 0.026
3.568 ± 0.032
3.99 ± 0.034
5.837 ± 0.038
5.69 ± 0.037
6.98 ± 0.036
0.87 ± 0.015
3.668 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here