Cupriavidus plantarum
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5711 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A316F5L5|A0A316F5L5_9BURK N-acetyl-gamma-glutamyl-phosphate reductase OS=Cupriavidus plantarum OX=942865 GN=argC PE=3 SV=1
MM1 pKa = 6.8 NTVEE5 pKa = 5.29 IPIPDD10 pKa = 4.46 PEE12 pKa = 5.2 DD13 pKa = 3.91 EE14 pKa = 4.98 PDD16 pKa = 3.15 LRR18 pKa = 11.84 SYY20 pKa = 11.06 INQFITDD27 pKa = 4.84 EE28 pKa = 4.2 IGSLYY33 pKa = 10.89 GEE35 pKa = 4.88 LVPALDD41 pKa = 4.67 LDD43 pKa = 4.21 VGDD46 pKa = 4.65 EE47 pKa = 4.14 RR48 pKa = 11.84 ATIDD52 pKa = 4.91 DD53 pKa = 4.06 IEE55 pKa = 5.2 VDD57 pKa = 3.78 DD58 pKa = 5.36 VEE60 pKa = 4.45 VHH62 pKa = 5.92 GDD64 pKa = 3.7 KK65 pKa = 10.66 IIVYY69 pKa = 7.43 YY70 pKa = 9.16 TIQYY74 pKa = 8.36 SAHH77 pKa = 6.14 YY78 pKa = 8.94 GCSDD82 pKa = 2.87 IDD84 pKa = 3.78 YY85 pKa = 11.27 AEE87 pKa = 4.98 DD88 pKa = 3.61 DD89 pKa = 3.49 QRR91 pKa = 11.84 KK92 pKa = 7.07 ITGVRR97 pKa = 11.84 DD98 pKa = 3.42 GEE100 pKa = 4.0 NWVFDD105 pKa = 3.83 WYY107 pKa = 11.21 VSPEE111 pKa = 3.49 RR112 pKa = 11.84 LAPNEE117 pKa = 3.96 EE118 pKa = 4.29 LL119 pKa = 4.43
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.808
Patrickios 0.985
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A316ES26|A0A316ES26_9BURK LysR family transcriptional regulator OS=Cupriavidus plantarum OX=942865 GN=C7419_103658 PE=3 SV=1
MM1 pKa = 7.32 RR2 pKa = 11.84 RR3 pKa = 11.84 ARR5 pKa = 11.84 RR6 pKa = 11.84 SRR8 pKa = 11.84 TTDD11 pKa = 2.46 ACIRR15 pKa = 11.84 RR16 pKa = 11.84 CRR18 pKa = 11.84 RR19 pKa = 11.84 IPHH22 pKa = 6.67 LSAPGGIRR30 pKa = 11.84 RR31 pKa = 11.84 CLPRR35 pKa = 11.84 CGEE38 pKa = 3.99 TSGRR42 pKa = 11.84 QARR45 pKa = 11.84 RR46 pKa = 11.84 ARR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 CRR52 pKa = 11.84 HH53 pKa = 4.73 HH54 pKa = 6.53 RR55 pKa = 11.84 HH56 pKa = 4.91 RR57 pKa = 11.84 HH58 pKa = 3.92 RR59 pKa = 11.84 HH60 pKa = 4.17 RR61 pKa = 11.84 HH62 pKa = 5.1 RR63 pKa = 11.84 QLQLQLQLQPQGRR76 pKa = 11.84 ARR78 pKa = 11.84 ALRR81 pKa = 11.84 PPPRR85 pKa = 11.84 PRR87 pKa = 11.84 VPVLLRR93 pKa = 11.84 GRR95 pKa = 11.84 AGRR98 pKa = 11.84 RR99 pKa = 11.84 TRR101 pKa = 11.84 RR102 pKa = 11.84 RR103 pKa = 11.84 ARR105 pKa = 11.84 VSPTTAATIATNGGHH120 pKa = 7.17 RR121 pKa = 11.84 ATIPRR126 pKa = 11.84 SPGRR130 pKa = 11.84 GASGKK135 pKa = 8.94 ISGRR139 pKa = 11.84 ARR141 pKa = 11.84 EE142 pKa = 3.92 HH143 pKa = 6.12 RR144 pKa = 11.84 RR145 pKa = 11.84 RR146 pKa = 11.84 ARR148 pKa = 11.84 RR149 pKa = 11.84 PRR151 pKa = 11.84 HH152 pKa = 4.91 RR153 pKa = 11.84 LRR155 pKa = 11.84 RR156 pKa = 11.84 RR157 pKa = 11.84 PHH159 pKa = 6.21 RR160 pKa = 11.84 RR161 pKa = 11.84 LHH163 pKa = 4.75 QLRR166 pKa = 11.84 HH167 pKa = 5.72 RR168 pKa = 11.84 RR169 pKa = 11.84 LHH171 pKa = 4.63 QLRR174 pKa = 11.84 LRR176 pKa = 11.84 LRR178 pKa = 11.84 LRR180 pKa = 11.84 LRR182 pKa = 11.84 LRR184 pKa = 11.84 RR185 pKa = 11.84 LHH187 pKa = 5.18 QRR189 pKa = 11.84 QRR191 pKa = 11.84 QRR193 pKa = 11.84 QRR195 pKa = 11.84 QHH197 pKa = 4.96 QRR199 pKa = 11.84 QHH201 pKa = 4.49 QRR203 pKa = 11.84 QHH205 pKa = 4.57 QRR207 pKa = 11.84 RR208 pKa = 11.84 HH209 pKa = 4.37 RR210 pKa = 11.84 HH211 pKa = 4.01 RR212 pKa = 11.84 HH213 pKa = 3.78 RR214 pKa = 11.84 HH215 pKa = 4.13 RR216 pKa = 11.84 HH217 pKa = 4.13 RR218 pKa = 11.84 HH219 pKa = 4.13 RR220 pKa = 11.84 HH221 pKa = 4.13 RR222 pKa = 11.84 HH223 pKa = 4.13 RR224 pKa = 11.84 HH225 pKa = 4.13 RR226 pKa = 11.84 HH227 pKa = 4.35 RR228 pKa = 11.84 HH229 pKa = 3.69 RR230 pKa = 11.84 HH231 pKa = 4.68 RR232 pKa = 11.84 PPRR235 pKa = 11.84 RR236 pKa = 11.84 TSRR239 pKa = 11.84 RR240 pKa = 11.84 SRR242 pKa = 11.84 TSPSPPAIPPHH253 pKa = 6.33 RR254 pKa = 11.84 SSPNTRR260 pKa = 11.84 RR261 pKa = 11.84 RR262 pKa = 11.84 WW263 pKa = 3.31
Molecular weight: 32.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.199
IPC_protein 12.808
Toseland 12.969
ProMoST 13.466
Dawson 12.969
Bjellqvist 12.969
Wikipedia 13.451
Rodwell 12.471
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.969
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.179
IPC_peptide 13.466
IPC2_peptide 12.457
IPC2.peptide.svr19 9.206
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5711
0
5711
1919394
27
3701
336.1
36.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.171 ± 0.047
0.852 ± 0.01
5.503 ± 0.031
5.063 ± 0.03
3.429 ± 0.02
8.453 ± 0.039
2.246 ± 0.019
4.583 ± 0.021
2.828 ± 0.028
10.157 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.531 ± 0.015
2.653 ± 0.022
5.378 ± 0.023
3.567 ± 0.024
7.301 ± 0.033
5.304 ± 0.023
5.548 ± 0.034
7.759 ± 0.027
1.335 ± 0.012
2.34 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here