Paracoccus sp. S-4012
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3506 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I2LZG6|A0A6I2LZG6_9RHOB Rhs element Vgr protein OS=Paracoccus sp. S-4012 OX=2665648 GN=GI374_12115 PE=4 SV=1
MM1 pKa = 7.47 SKK3 pKa = 10.25 FRR5 pKa = 11.84 NAVSLLVLLAASPAAAQSDD24 pKa = 4.46 GSPVPAAPSAEE35 pKa = 4.05 AKK37 pKa = 10.51 ASAPLGIGRR46 pKa = 11.84 TALPEE51 pKa = 4.49 EE52 pKa = 3.93 IAAWDD57 pKa = 3.95 KK58 pKa = 11.31 DD59 pKa = 3.46 ISPDD63 pKa = 4.02 GLGLPEE69 pKa = 4.46 GSGDD73 pKa = 3.78 VASGEE78 pKa = 4.27 ALFTDD83 pKa = 4.78 YY84 pKa = 10.9 CASCHH89 pKa = 6.33 GDD91 pKa = 3.28 FAEE94 pKa = 6.47 GLDD97 pKa = 3.78 NWPALAGGEE106 pKa = 4.35 GTLDD110 pKa = 3.44 HH111 pKa = 7.91 DD112 pKa = 5.31 DD113 pKa = 3.91 PEE115 pKa = 4.45 KK116 pKa = 10.57 TIGSYY121 pKa = 8.64 WPYY124 pKa = 11.18 LSTVWDD130 pKa = 3.88 YY131 pKa = 9.93 VHH133 pKa = 7.42 RR134 pKa = 11.84 SMPFGNAQSLTADD147 pKa = 3.25 EE148 pKa = 5.19 TYY150 pKa = 10.83 AITAYY155 pKa = 9.63 ILYY158 pKa = 10.44 SNYY161 pKa = 10.2 LVEE164 pKa = 6.38 DD165 pKa = 4.63 DD166 pKa = 4.31 FTLSHH171 pKa = 6.35 TNFGEE176 pKa = 4.23 VEE178 pKa = 4.14 LPNAGGFVEE187 pKa = 5.03 DD188 pKa = 5.19 DD189 pKa = 3.61 RR190 pKa = 11.84 AEE192 pKa = 3.95 TEE194 pKa = 4.1 YY195 pKa = 10.35 PIFAGEE201 pKa = 4.03 PCMEE205 pKa = 4.19 NCKK208 pKa = 10.57 DD209 pKa = 3.27 AVEE212 pKa = 4.36 ITKK215 pKa = 9.97 HH216 pKa = 6.18 ASVLDD221 pKa = 3.6 VTPEE225 pKa = 4.3 DD226 pKa = 4.61 GSDD229 pKa = 3.38 APEE232 pKa = 4.36 GAEE235 pKa = 4.5 GMM237 pKa = 4.3
Molecular weight: 25.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 0.896
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.872
Protein with the highest isoelectric point:
>tr|A0A6I2M0E2|A0A6I2M0E2_9RHOB Magnesium transporter MgtE OS=Paracoccus sp. S-4012 OX=2665648 GN=mgtE PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.73 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.98 GRR39 pKa = 11.84 RR40 pKa = 11.84 VLSAA44 pKa = 3.76
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3506
0
3506
1100487
14
2855
313.9
33.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.201 ± 0.072
0.786 ± 0.012
5.616 ± 0.036
6.039 ± 0.037
3.335 ± 0.023
9.267 ± 0.044
2.015 ± 0.02
4.624 ± 0.029
2.074 ± 0.031
10.385 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.021
2.066 ± 0.023
5.77 ± 0.033
2.844 ± 0.024
8.091 ± 0.046
4.563 ± 0.028
5.101 ± 0.028
7.317 ± 0.032
1.479 ± 0.018
1.859 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here