Gallaecimonas xiamenensis 3-C-1
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3798 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K2JXC7|K2JXC7_9GAMM M20_dimer domain-containing protein OS=Gallaecimonas xiamenensis 3-C-1 OX=745411 GN=B3C1_14545 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.11 KK3 pKa = 8.29 TLTLALTSAAALVLTACGGSSGGDD27 pKa = 3.37 DD28 pKa = 3.53 TPSPQTARR36 pKa = 11.84 LSISIGDD43 pKa = 4.13 APVDD47 pKa = 3.37 TADD50 pKa = 3.59 QVVVTIDD57 pKa = 3.53 NIVLKK62 pKa = 10.82 RR63 pKa = 11.84 EE64 pKa = 4.13 GEE66 pKa = 4.04 EE67 pKa = 4.41 DD68 pKa = 3.5 VVLPVRR74 pKa = 11.84 DD75 pKa = 4.13 ADD77 pKa = 4.0 NQPVTLDD84 pKa = 3.53 LLDD87 pKa = 3.9 YY88 pKa = 11.0 QGGDD92 pKa = 3.06 VFVALNGVTIPAGTYY107 pKa = 9.75 SDD109 pKa = 3.73 VRR111 pKa = 11.84 LDD113 pKa = 4.49 ILDD116 pKa = 4.15 EE117 pKa = 4.37 DD118 pKa = 4.43 TANSYY123 pKa = 10.94 VDD125 pKa = 3.51 ADD127 pKa = 4.0 GGIHH131 pKa = 5.65 EE132 pKa = 5.45 LKK134 pKa = 10.88 VPSDD138 pKa = 3.65 EE139 pKa = 4.63 LKK141 pKa = 11.19 LGGFDD146 pKa = 3.74 AAAGGQLAFTIDD158 pKa = 3.49 FNLRR162 pKa = 11.84 KK163 pKa = 10.18 AMTYY167 pKa = 10.53 NPGPDD172 pKa = 3.13 RR173 pKa = 11.84 YY174 pKa = 9.46 ILKK177 pKa = 10.03 PRR179 pKa = 11.84 GIQLLEE185 pKa = 3.85 TAILGQIGGEE195 pKa = 3.94 VDD197 pKa = 3.08 PALAEE202 pKa = 4.22 SCNTGTDD209 pKa = 3.16 NVNYY213 pKa = 10.0 GFVYY217 pKa = 10.21 LYY219 pKa = 10.47 SGHH222 pKa = 7.54 DD223 pKa = 3.89 LTTLADD229 pKa = 3.82 DD230 pKa = 4.81 HH231 pKa = 7.06 DD232 pKa = 4.33 QGAAGAPEE240 pKa = 4.56 GASIPVASMAVTLSDD255 pKa = 4.27 DD256 pKa = 4.26 ADD258 pKa = 4.02 PATTDD263 pKa = 3.05 PYY265 pKa = 10.25 TYY267 pKa = 10.95 KK268 pKa = 10.66 FGLLMPGDD276 pKa = 3.7 YY277 pKa = 10.17 TLAFSCDD284 pKa = 3.95 GINDD288 pKa = 3.78 LPEE291 pKa = 5.38 SYY293 pKa = 10.5 EE294 pKa = 3.9 GLTIPNPEE302 pKa = 4.0 GLSYY306 pKa = 11.0 EE307 pKa = 4.22 LTLTDD312 pKa = 5.74 GGDD315 pKa = 3.4 LTQDD319 pKa = 3.13 INSLSTGLL327 pKa = 3.8
Molecular weight: 34.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.126
Thurlkill 3.541
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|K2JLP1|K2JLP1_9GAMM Smr domain-containing protein OS=Gallaecimonas xiamenensis 3-C-1 OX=745411 GN=B3C1_04950 PE=4 SV=1
MM1 pKa = 7.14 QRR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 9.2 RR14 pKa = 11.84 DD15 pKa = 3.06 HH16 pKa = 6.58 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.96 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3798
0
3798
1224611
36
2797
322.4
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.088 ± 0.051
0.934 ± 0.015
5.879 ± 0.039
5.332 ± 0.038
3.703 ± 0.028
8.122 ± 0.038
2.149 ± 0.021
4.252 ± 0.031
4.154 ± 0.035
12.322 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.298 ± 0.02
3.002 ± 0.027
4.705 ± 0.029
5.137 ± 0.035
5.562 ± 0.037
5.687 ± 0.035
4.574 ± 0.031
6.851 ± 0.031
1.533 ± 0.017
2.717 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here